96 results about "Optical biosensor" patented technology

Methods are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also relates to optical devices.

The instant invention provides compositions and methods for determining cell interactions that are faster than conventional methods and that require the use of fewer reagents than conventional methods.

An optical biometric sensor includes an array of light sensing elements, an array of diverging optical elements, and an array of apertures disposed between the array of light sensing elements and the array of diverging optical elements. Light incident on the diverging optical elements within a limited acceptance angle passes through the apertures and towards the light sensing elements and light incident on the diverging optical elements outside of the limited acceptance angle diverges away from the apertures.

An optical biosensor has a first enclosure with a pathogen recognition surface, including a planar optical waveguide and grating located in the first enclosure. An aperture is in the first enclosure for insertion of sample to be investigated to a position in close proximity to the pathogen recognition surface. A laser in the first enclosure includes means for aligning and means for modulating the laser, the laser having its light output directed toward said grating. Detection means are located in the first enclosure and in optical communication with the pathogen recognition surface for detecting pathogens after interrogation by the laser light and outputting the detection. Electronic means is located in the first enclosure and receives the detection for processing the detection and outputting information on the detection, and an electrical power supply is located in the first enclosure for supplying power to the laser, the detection means and the electronic means.

Methods are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also relates to optical devices.

The invention relates to compositions and methods for detecting biomolecular interactions. The detection can occur without the use of labels and can be done in a high-throughput manner. The invention further relates to self-referencing colorimetric resonant optical biosensors and optical devices.

The invention relates to compositions and methods for detecting biomolecular interactions. The detection can occur without the use of labels and can be done in a high-throughput manner. The invention further relates to self-referencing colorimetric resonant optical biosensors and optical devices.

An evanescent-wave optical biosensor includes a hollow optical waveguide, preferably in the form of a light-conductive capillary, surrounding a central waveguide preferably in the form of an optical fiber to create a sealed cavity. A source of optical energy as from a laser is directed into one or both of the light-input ends of the capillary and fiber, such that an evanescent field extends into the cavity from one or both of the inner surface of the capillary and the outer surface of the fiber. A first biomolecular constituent is attached to one or both of the inner wall of the hollow optical waveguide and the outer surface of the second optical waveguide, such that the first biomolecular binding partner is substantially within the evanescent field if present. A first optoelectric detector is supported to receive light from the light-output end of the capillary and convert the light received into a first electrical signal, and a second optoelectric detector is supported to receive light from the light-output end of the fiber and convert the light received into a second electrical signal. A fluid within the cavity which may contain a second biomolecular constituent having a binding affinity to the first biomolecular constituent, such that if binding occurs between the biomolecular constituents, a representative change occurs in the light emerging from one or both of the output ends of the hollow and second optical waveguides and the electrical signals from the optoelectric detectors.

Ear-mounted apparatus are provided for secure and comfortable load bearing in support of optical, biosensor, and mobile communication functionalities. In particular, a load-bearing ear unit is provided which includes an anchor for stable attachment in an ear and a member extending from the anchor. The member has therein a rotational stabiliser adapted to provide stability in relevant planes for secure ear mounting. Also provided is an ear-mounted optical device, comprising the load-bearing ear unit and one of a camera, a light source, a virtual reality device, a IrDA-device, and a photo diode. Further provided is an ear-mounted wearable device, comprising the load-bearing ear unit and one or more biosensors. A particular such wearable device is an ear-mounted EAG device.

The invention provides an optical biosensor used for detecting surface cleanliness rapidly, and a testing method thereof. The sensor comprises a sampling stick, a detection cavity, a liquid storage tank and a reaction chamber. The sampling stick comprises a handle, a spacing ring, a sampling swab and a pointed casing; the liquid storage tank is provided with a plurality of uniform sealing pieces which are in fixed connection through screw threads, and an ATP extractant and a neutralization reagent used for neutralizing the remaining extractant are sealed therein; and the lateral wall of the reaction chamber allows light to pass through, and a sensitive reagent is fixed at the bottom part in advance. When in detection, firstly, the sampling swab of the sampling stick is used for daubing and sampling the surface of an object to be detected; then, the pointed casing is screwed downwards; the sampling stick is inserted into the sampling detection cavity; and with the consideration of the fluorescence strength during the detection of a sample by an optical device, the surface cleanliness of the object to be tested can be determined by comparing the light intensity value with the standard sanitary threshold value. The sensor provided by the invention has the advantages of simple operation, convenient use, rapid response and disposability, and can be widely applied to the object surface cleanliness real-time detection and the field hygiene supervision in industries, such as food processing, cosmetics production, medical service and the like.

An optical biosensor that is capable of reading a fingerprint or a vein pattern with high efficiency of light reception. A louver is provided between a backlight unit and a substrate in a sensor for identifying a fingerprint by a process in which light emitted from the backlight unit passes through the substrate and is radiated to a fingerprint that is in contact with the upper surface of a protective layer, and light that is reflected according to the surface irregularity of the fingerprint is received by a semiconductor layer that is a light-receiving element. The emitted light is thereby provided with sharp directivity, and the efficiency of light reception by the sensor is therefore increased.

The invention discloses a fabrication method of an optical micro-nano biosensor integrated with a microfluidic system. The method comprises the steps that a substrate is cleaned; a micro-nano slab-guide optical device is fabricated through photoetching, developing, etching, and the like on the top layer of the substrate; the surface of the device is coated with SU-8 thick glue in a spinning manner; an SU-8 glue microfluidic channel is obtained through alignment exposure, postbaking, developing, and the like; an SU-8 layer is coated and permanently bonded with a PDMS (polydimethylsiloxane) sheet, so that the integration of the micro-nano slab-guide optical device with a microfluidic is achieved; a relevant reagent is injected from a liquid inlet; chemical modification of a micro-ring region is completed; a reagent solution to be tested is injected; and a function of on-chip biological detection is achieved by observing variations of optical power or resonance. The method has the advantages of simplicity in operation and high reliability, and is suitable for fabricating the microfluidic systems of the most slab-guide micro-nano optical biosensors.

A method for performing a blood assay includes the steps of positioning an optical biosensor in fluid communication with a blood vessel whereby blood from the blood vessel contacts the biosensor. The biosensor includes at least one material adapted to bind to an analyte. The method also includes the steps of detecting a change in at least one optical property of the biosensor resulting from binding of the at least one material with the analyte and transmitting a continuous signal representative of the change in at least one optical property of the biosensor to a display module to provide real time analysis by a clinician.

An optical biosensor pixel for detecting the amount of light that is generated by the biosensing process and a biosensor array architecture that includes such biosensor pixels. The optical biosensor pixel includes a photodiode configured to convert an incident photon flux into a current. Additionally, the optical biosensor pixel includes an optical filter configured to select specific wavelengths and / or photon flux angles to reach the photodiode from a biological sample. The biosensor pixel further includes a trans-impedance amplifier coupled to the photodiode, where the trans-impedance amplifier is configured to convert the current into a voltage signal. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

The invention discloses an optical biosensor for detecting brain natriuretic peptide (BNP) and a preparation method of a reagent thereof, relating to the biosensing technology. A sensor reaction zone is a capillary channel filled with magnetic nano probe solution, the inner surface of the channel is assembled with a layer of hydrophilic nano material, the upper surface of the channel is an euphotic insulating encapsulation layer with an injection hole, the side surface of the channel is encapsulated by plastics, the two ends thereof encapsulated with plastics are respectively provided with a controllable electromagnet. The preparation method of the reagent is as follows: preparing magnetic nano probes, fully reacting the magnetic nano probes, a brain natriuretic peptide (BNP) sample to bedetected and specificity BNP antigen marked by a marker under the united action of the electromagnet and the capillary channel, forming a compound of magnetic nano probes, BNP antigen to be detected and marked antibody, and adding substrate for catalytic luminescence or exciting luminescent quantum dots for luminescence. By the sensor detection system, the BNP concentration can be rapidly detected. The sensor in the invention can rapidly detect the BNP concentration in a sample, and has the characteristics of high specificity, high sensitivity, a great quantity of saved biological reagent andthe like.

The invention relates to focusing optics (100) for a biosensor (10) which allow with simple means to accurately image an extended investigation region (13) onto a detector plane (P). To this end, the focusing optics (100) comprises at least two focusing lenslets (LL) that are arranged adjacent to each other such that they image an incident parallel light beam (L2) that is directed along a main optical axis (MOA) onto a common plane (P). The output light beam (L2) that is received by the focusing optics (100) may preferably originate from total internal reflection of a parallel input light beam (L1) at the investigation region (13) of a transparent carrier.

A distal tip, and associated method of producing, of a biosensor ion sensing transducer for use in detecting an analyte comprising an halogenated organic compound in an environment such as soil or an aqueous environment. Features include: a biocomponent comprising at least one enzyme for carrying out a dehalogenation of the compound; the biocomponent is immobilized to a surface of the tip; a treatment of the biocomponent for maintaining a period of enzymatic efficacy; and the biocomponent stabilized by means preferably selected from the group consisting of crosslinking a surface of the immobilized biocomponent, crosslinking a polymer layer to the biocomponent, adding a gel-hardening agent to the biocomponent, adding a stabilizing agent to the biocomponent, and modifying a component of the immobilizing means. Immobilization can be carried out by: (a) entrapment within a hydrogel; (b) entrapment within a polymeric network; (c) (micro)encapsulation; (d) covalent-bonding; and (e) adsorption. The dehalogenase of the biocomponent may be selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5 as listed in Table 2A.

The invention relates to the field of surface waves based optical devices particularly tuneable optical filter, optical biosensors and spatial light modulators. An optical sensor and tuneable filter is disclosed based on high contrast periodic structures deposited on a substrate and using a compact reading method for low detection limit using a one dimensionally diverging quasi-monochromatic beamand a camera.

The invention discloses a dual-ring resonant cavity-based Mach-Zehnder interferometer optical biosensor, comprising a laser light source, a Mach-Zehnder interferometer, two ring-shaped waveguide resonant cavities and two detectors; the surface of a core layer of the first ring-shaped waveguide resonant cavity is modified with a biological antibody with a specific adsorption function; the surface of a core layer of the first ring-shaped waveguide resonant cavity is modified with a competition antigen for adsorbing the biological antibody; after a to be detected antigen in to be detected liquid is combined with the biological antibody, the effective refractive index of the first ring-shaped waveguide resonant cavity is caused to be increased, while the effective refractive index of the second ring-shaped waveguide resonant cavity is reduced, so that phase difference of inside light in the two ring-shaped waveguide resonant cavities is increased, the change of the phase difference is converted into the change of output power through the Mach-Zehnder interferometer, and the content of the to be detected antigen in the to be detected liquid is obtained by measuring the ratio of output powers of two ports of the Mach-Zehnder interferometer, thus greatly reducing the requirement on light source power stability.

The present provides optical sensor based sensitive, label-free binding assay methods and kits for isothermal real-time detection of the binding of specific analytes (such as nucleic acids, proteins and low molecular weight antigenic or receptor binding ligands) present in low amount in different biological samples. In the binding assays of the invention, the analyte is captured at the specifically pretreated solid surface of optical biosensors in specific recognition reactions (such as hybridization, specific protein-protein interactions and receptor-ligand interactions). The specificity of the methods of the invention is further enhanced by a second specific recognition step using a padlock probe comprising an indicator sequence designed to keep the products of a subsequently performed isothermal nucleic acid amplification method (e.g. rolling circle amplification: RCA) anchored on the sensor surface, enhancing thereby the sensitivity of the detection of the specific binding of the analyte occurred on the sensor surface.

The present invention relates to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing corneal dystrophy, and more particularly, to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays which uses the optical characteristics of localized surface plasmon resonance (LSPR), to a method for producing the chip, and to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and various types of corneal dystrophies. According to the present invention, the metal-deposited nucleic acid chip with nanostructure arrays, and an analysis device including a light source, a detector, a spectrophotometer and a computer are combined to be used as a label-free biosensor based on the optical characteristics of LSPR. The multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and corneal dystrophy according to the present invention diagnoses various types of corneal dystrophies, which are hereditary eye diseases, at the same time.

An electricity-optics joint urine analysis biochemical system and a production method thereof relate to functional materials for biosensors. The system is provided with a urine reaction cell, an LED (light-emitting diode) light source, an optical fiber spectrometer, a singlechip and a display circuit; an optical biosensor, an electrochemical biosensor and a pH (Potential Of Hydrogen) meter are arranged in the reaction cell, the upper end of the reaction cell is provided with a urine inlet, the bottom of the reaction cell is provided with a urine outlet, and a modified electrode and a reference electrode of the electrochemical biosensor are arranged on a lower protective jacket end of the urine reaction cell; the output end of the optical biosensor is connected with the input end of the optical fiber spectrometer, the output end of the optical fiber spectrometer is connected with the singlechip, the output end of the electrochemical biosensor is connected with the singlechip, and the output end of the pH meter is connected with the singlechip; and the singlechip is connected with the display circuit. The polypyrrole / platinum nano-hair-based modified porous alumina foil electrode is first prepared, the bipyridyl ruthenium fluorescence quenching reaction-based optical biosensor is then prepared, and finally, the electricity-optics joint urine analysis biochemical system is constructed.

The instant invention provides compositions and methods for determining cell interactions that are faster than conventional methods and that require the use of fewer reagents than conventional methods.