29 results about "Optical biosensor" patented technology

The invention relates to the technical field of biosensing, and discloses an ultra-sensitive biosensor as well as a preparation method and application thereof. The ultra-sensitive biosensor comprises a substrate, a two-dimensional material loaded on the substrate, DNA origami loaded on the two-dimensional material and quantum dots modified on the DNA origami, wherein the DNA origami contains an exposed single-stranded DNA fragment, and the single-stranded DNA fragment can be cut by an activated Cas protein; the quantum dots are connected with the tail ends of the single-stranded DNA fragments. The ultrasensitive biosensor is excellent in performance, and compared with a conventional pure optical biosensor combining surface plasma resonance and DNA origami, the sensitivity of the ultrasensitive biosensor is improved by four orders of magnitude and can reach the aM level.

The invention discloses a high-sensitivity optical biochip based on an AAO and ZIF-8 composite structure and a preparation and detection method thereof, and relates to the technical field of optical biochip detection. The preparation method comprises the following steps: coating the surface of nanopore aluminum oxide with ZIF-8 to obtain a ZIF-8 layer on the surface of the nanopore aluminum oxide, then carrying out carboxyl or amino modification to obtain a functionalized ZIF-8 layer, and then fixing a specific antibody on the surface of the functionalized ZIF-8 layer by using a cross-linking agent to obtain the optical biosensor. According to the sensor, an AAO nanopore array serves as a structural substrate, a high-activity MOF functional layer is integrated, ultra-sensitive detection is achieved through the precise pore channel confinement effect and the molecular recognition technology, and the prepared optical biosensor has the advantages of being low in cost, high in stability, short in detection time, high in detection sensitivity and specificity and the like.

The invention discloses a laccase gene expression cassette, a laccase optical biosensor platform and a construction method and application of the laccase optical biosensor platform. The expression cassette comprises the following expression elements which are connected in sequence: (a) a Trichoderma reesei-derived Pcbh1 promoter; (b) an exogenous laccase coding gene; and (c) a Tcbh1 terminator derived from Trichoderma reesei. The invention has the following beneficial effects: the AALB platform realizes sensitive, economical and efficient screening of transcription factor variants by constructing a laccase gene expression cassette which is derived from oyster mushroom and is regulated by a Pcbh1 promoter; according to the invention, the high-activity exogenous laccase gene is integrated at the ACE1 site of the Trichoderma reesei genome, so that the regulation effect of the transcription factor is amplified, and the expression level of laccase can intuitively reflect the transcriptional activity of the transcription factor.

The present disclosure relates to an integrated chip including a semiconductor layer and a photodetector disposed along the semiconductor layer. A color filter is over the photodetector. A micro-lens is over the color filter. A dielectric structure comprising one or more dielectric layers is over the micro-lens. A receptor layer is over the dielectric structure. An optical signal enhancement structure is disposed along the dielectric structure and between the receptor layer and the micro-lens.

The invention is applicable to the technical field of optical biosensors, and provides an optical biosensor with a single ring-double ring mode switching function, which comprises a broadband light source, an optical signal is transmitted to an optical switch I, an optical sensing chip is respectively connected with the optical switch I and an optical switch II through an optical fiber array, and the optical switch II is connected with a spectrum analyzer. The optical fiber array is connected with the optical sensing chip and the optical switch and used for controlling switching and detection of a single-ring mode or a double-ring mode, when liquid to be detected causes high absorption of the sensing ring, the single-ring sensing mode is adopted, otherwise, the double-ring sensing mode is adopted, and when double-ring sensing is adopted, the single-ring sensing mode is adopted. The reference ring is tuned at the two ends of the thermode of the reference ring, the effective refractive index of the reference ring is changed, when the change of the refractive index is small, characterization of a to-be-measured substance is achieved through the spectral envelope drift distance, when the change of the refractive index is large, characterization of the to-be-measured substance is conducted through the change of FSR, and high-sensitivity and large-range sensing is achieved.

The invention is a method for training a system adapted for classifying biosensor signal corresponding to cell, wherein o the system comprises a machine learning-based classification module, and o sensor-surface time-dependent biosensor signals (26) obtained by contacting a sample with cell(s) (32) with functionalised surface of an optical biosensor (24) for detecting refractive index change, representing activation kinetics and defined on pixels (30) of the biosensor (24) are applied, the method comprising, for the sensor-surface time-dependent biosensor signals (26), - a training time-dependent biosensor signal portion comprising a pixel of the given sensor-surface time-dependent biosensor signal (26) corresponding to a cell (32) is determined, and - the classification module is trained with the training time-dependent biosensor signal portion, classification of activation kinetics of the cell (32) is specified. Furthermore, the invention is a system and an evaluation method for classifying biosensor signal corresponding to cell.

MXenes are nanostructures with unique characteristics for bioanalytical applications, such as hydrophilicity, large surface area, strong metallic conductivity, strong ion transport capabilities, biocompatibility, minimal diffusion barrier, and easy functionalization. These materials are formed of transition metallic nitrides, carbides, or carbonitrides. This invention is the innovative of using MXenes in electrochemical and optical biosensors, identifying future requirements and potential in this sector, particularly in the development of wearable sensors and platforms with integrated biomolecule detection.

The utility model belongs to the technical field of optical biosensors, and particularly relates to an enhanced Fabry-Perot microcavity sensor for ultralow-concentration biological detection. The sensing microcavity comprises a square capillary quartz tube, wherein high-reflectivity films are plated on the upper surface and the lower surface of the square capillary quartz tube; a liquid core area of the square tube is a micro-flow channel, and a polyethylene glycol polymerization promoting biomolecule solution is introduced into the micro-flow channel, so that the Fabry-Perot microcavity biosensor is obtained. According to the utility model, the square capillary quartz tube provides high-precision parallelism between the two reflectors, so that the diffraction and walk-off loss can be reduced, the mode volume in the cavity can be reduced, the light energy density can be increased, the strong interaction between light and substances can be realized, and the quality factor can be improved; polyethylene glycol can promote the polymerization of biomolecular protein, enhance the reaction of an antigen-antibody compound, and further promote the aggregation of an immune compound. The biosensor is simple in structure, convenient to prepare, good in repeatability and stability, high in response speed and free of surface modification and chemical labeling.

The invention is directed to a surface-functionalized optical element comprising an IR-transparent optical element, wherein at least a part of the surface of the IR-transparent optical element is covalently bound to a molecular probe by a linker construct. The linker construct comprises a surface attachment moiety, and a spacer moiety which comprises a poly(alkylene oxide). The invention further relates to a process for preparing said surface-functionalized optical element, to an optical biosensor comprising said surface-functionalized optical element, and to a method for detecting a target analyte which uses said surface-functionalized optical element.

The invention discloses a singularity enhancement effect-based multi-detection optical biosensor, which comprises a microfluidic structure and a sensing chip, the sensing chip comprises a sensing layer, the sensing layer is modified with a specific antibody, the microfluidic structure introduces a to-be-detected liquid into the sensing layer, and the to-be-detected liquid is combined with the specific antibody at the sensing layer, so that the to-be-detected liquid is detected. The sensing layer is realized based on a micro-ring structure of a non-Hermite singular point, when liquid to be detected is not introduced into the sensing layer, the sensing layer is in a second-order singular point state, and when the liquid to be detected is introduced into the sensing layer and is combined with a specific antibody, the second-order singular point state of the sensing layer is broken, a resonance peak in a spectrum is split, and the specific antibody is detected. The relationship between the splitting degree and the concentration of the target substance in the to-be-detected liquid is established through splitting of the harmonic peak, and quantitative detection of the target substance in the to-be-detected liquid is achieved; the method has the advantages that the method does not depend on a high Q value, can also have high sensitivity and low detection limit, can simultaneously cover ultralow concentration detection requirements of a ng / ml level, a pg / ml level and an fg / ml level, and is wider in application scene.

The invention relates to a fully-integrated microfluidic optical biosensing test system, which comprises a computer module, a fully-integrated optical biosensing test module and a microfluidic sensing chip module, and is characterized in that the fully-integrated optical biosensing test module comprises a broadband light source, a micro spectrometer, a microfluidic pump and a stepping motor control module; the computer module is connected with the broadband light source and the micro spectrograph through communication interface connecting lines, and the stepping motor control module is connected with the micro-flow pump; and the micro-fluidic sensing chip module is connected with the broadband light source and the micro spectrograph through the output optical fiber. According to the invention, the volume limitation of the traditional optical equipment is reduced on the hardware architecture, the high-precision micro-flow pump carried by the system realizes the precise transportation control of the micro-upgrading fluid, and the detection repeatability and the result reliability of the complex biological sample are obviously improved.

The invention discloses a blood pressure change recognition method and device, wearable equipment and a storage medium. The blood pressure change identification method comprises the following steps: acquiring target sign data acquired by an optical biosensor in a detection time period; performing spectrum analysis on the target sign data to obtain an optical spectrum feature sequence corresponding to the target sign data; according to the target feature mapping relation and the optical spectrum feature sequence, blood pressure change information corresponding to the detection time period is determined; the target feature mapping relation is used for representing a mapping relation between a first optical spectrum feature corresponding to the first sign data collected by the optical biosensor and a pressure spectrum feature corresponding to the pressure data collected by the pressure sensor under the condition that the pressure applying piece applies pressure to the human body. According to the blood pressure change recognition method and device, the wearable equipment and the storage medium, the accuracy of the blood pressure change information determined by the wearable equipment can be improved.

A multi-sensor optical biosensor system to determine one or more health parameters of a user, such as the user's blood pressure. The system includes a multi-sensor wearable device communicatively connected to a user compute device, which may be in communication with a remote compute device. The multi-sensor wearable device could include optical devices to generate a photoplethysmogram ("PPG") signal that is transmitted to the user compute device. In some cases, the multi-sensor wearable device may be configured to select a PPG channel to transmit to the user's compute device from a plurality of PPG channels based on one or more selection parameters.

MXenes are nanostructures with unique characteristics for bioanalytical applications, such as hydrophilicity, large surface area, strong metallic conductivity, strong ion transport capabilities, biocompatibility, minimal diffusion barrier, and easy functionalization. These materials are formed of transition metallic nitrides, carbides, or carbonitrides. This invention is the innovative of using MXenes in electrochemical and optical biosensors, identifying future requirements and potential in this sector, particularly in the development of wearable sensors and platforms with integrated biomolecule detection.

The invention relates to a Bloch surface wave-based high-sensitivity optical biosensor and a detection method thereof. The Bloch surface wave-based high-sensitivity optical biosensor comprises an optical substrate, a multilayer film formed by periodic photonic crystals, a surface functionalized layer and a microfluidic module from bottom to top, by controlling the thickness and refractive index distribution of the multilayer film, the sensor supports a BSW resonance mode at a specific wave band. According to the invention, by constructing a multilayer film formed by periodic photonic crystals, high-Q-value resonance excitation of BSW at 1550nm is realized, and a surface functionalized layer is constructed on the top layer and is used for capturing cancer-related target genes. According to the invention, fine regulation and control of BSW excitation conditions and controllable matching of the interface refractive index are realized.

The present invention provides a molecular probe-functionalized element comprising a solid substrate element, a first layer comprising linker A, a second layer comprising a peptide compound, a third layer comprising linker B, and a fourth layer comprising a molecular probe, wherein the peptide compound is covalently bonded to the solid substrate element via linker A and the molecular probe is covalently bonded to the peptide compound via linker B. The present invention also provides a process for preparing a molecular probe-functionalized element and an apparatus comprising the molecular probe-functionalized element such as an optical biosensor. Further, the present invention provides a method for detecting a biomarker, comprising: a) contacting the molecular probe-functionalized element with a sample suspected of containing a target analyte; and b) detecting the biomarker based on an interaction between the molecular probe and the target analyte. The present invention also provides the use of the molecular probe-functionalized element or apparatus for one or more of a companion diagnostic test; diagnosis of Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, diabetes, Huntington's, prion disease or tumors in a patient; monitoring the therapy of a patient having Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, diabetes, Huntington's, prion disease or tumors; and screening for drugs for the treatment of Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, diabetes, Huntington's, prion disease or tumors.

The present invention belongs to the field of biological detection technology, and specifically relates to a biosensor for detecting exosomes based on double rolling circle separation, comprising: template CT1 with nucleotide sequences shown in SEQ NO: 1-10, template CT2, Connector, DNA-chol, CP1, CP2, hairpin probe H1, hairpin probe H2, Reporter, crRNA, and Cas12a, T4 DNA ligase; the 3'-end of the DNA-chol is modified with cholesterol; the 5'-end and 3'-end of the Reporter are respectively connected with a fluorescent reporter group and a fluorescent quenching group. This sensor has the advantages of fast detection speed, simple operation, low price, low detection limit, high specificity, etc.

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

The invention discloses a blood pressure assessment method and device, wearable equipment and a storage medium, and is applied to the wearable equipment which comprises an air pump, an air bag, a pressure sensor and an optical biosensor. The method comprises the steps that an air pump is controlled to adjust the pressure of an air bag, a first physical sign signal of a target user is collected through an optical biosensor, and a pressure signal is collected through a pressure sensor; determining physical sign data according to a first physical sign signal collected by the optical biosensor; determining air bag pressure data according to a pressure signal collected by a pressure sensor; fusing the air bag pressure data and the physical sign data to obtain fused data; a target pressure curve is generated according to the fusion data, the first blood pressure evaluation value is determined according to the target pressure curve, and the reliability and accuracy of blood pressure evaluation can be improved.

This invention discloses a highly sensitive optical biochip based on an AAO and ZIF-8 composite structure, and its preparation and detection method, belonging to the field of optical biochip detection technology. In this invention, ZIF-8 is coated onto the surface of nanoporous alumina to obtain a ZIF-8 layer. Then, carboxyl or amino group modification is performed to obtain a functionalized ZIF-8 layer. Subsequently, a crosslinking agent is used to immobilize specific antibodies on the surface of the functionalized ZIF-8 layer, resulting in the optical biosensor. This sensor uses an AAO nanopore array as the structural substrate and integrates a highly active MOF functional layer. It achieves ultrasensitive detection through precise "pore confinement effect" and "molecular recognition technology." The optical biosensor prepared by this invention has advantages such as low cost, high stability, short detection time, and high detection sensitivity and specificity.

Various implementations of a system and method for analyzing extracellular vesicles (EVs) are disclosed having a number of innovative features. In one implementation, a method for analyzing EVs includes binding EVs to an optical waveguide biosensor and phenotyping the bound EVs. Phenotyping can include binding a labeled ligand to the EVs and / or rupturing the EVs and analyzing their cargo. In another implementation, a system for analyzing EVs includes EVs bound to an optical waveguide biosensor and a labeled ligand bound to the EVs. In another implementation, a kit for analyzing EVs includes a micro plate having wells containing optical waveguide biosensors functionalized with a binding agent configured to bind to EVs and at least one of: (a) labeled ligands configured to bind to the extracellular vesicles or (b) a reagent configured to rupture the extracellular vesicles.

The invention discloses a CMOS compatible biosensor based on a two-dimensional semiconductor and a preparation method and application of the CMOS compatible biosensor, specifically, an aluminum nitride layer compatible with a CMOS process is deposited on a silicon substrate, a two-dimensional semiconductor layer is formed on the surface of the aluminum nitride layer to serve as a sensing layer, and meanwhile a biological recognition element is fixed. The two-dimensional semiconductor layer is used for being combined with target biomolecules to generate a reaction product with electron donating and receiving capability, so that the reaction product is subjected to efficient charge transfer on the two-dimensional semiconductor layer, and the conductive detection layer can output sensitive and controllable signal change; the band gap characteristic and the high surface area of the two-dimensional semiconductor are utilized, the aluminum nitride and the silicon substrate are combined for use to achieve CMOS compatibility of the biosensing device, and due to the fact that the two-dimensional semiconductor has the excellent optical absorption and emission characteristics, the biosensing device can also be expanded to optical biosensing, optical biosensing and the like. Coupling detection of photoelectric signals and chemical or biological events is achieved, and the application range and multifunctionality are further expanded.

The application discloses a modification strategy for inorganic nanoparticles with controllable grafting proportion of multiple ligands on the surface of the inorganic nanoparticles, which can realize the modification of two or more different polymer ligands on the surface of the inorganic nanoparticles according to a specified grafting proportion. The method comprises the following steps: a) synthesizing an initiator with a structure capable of realizing mutual connection, wherein the initiator contains at least one initiating group and at least two mutually reactive groups; b) preparing different kinds of polymers with different molecular weights through atom transfer radical polymerization; c) connecting the different kinds of polymers with different molecular weights according to the designed proportion through the mutually reactive sites on the initiator; and d) modifying the prepared multi-component ligands on the surface of the nanoparticles through ligand exchange or chemical reaction. The modification strategy is suitable for nanoparticles with different sizes and materials and polymer ligands with different kinds and molecular weights. The obtained multi-ligand modified nanoparticles can realize different functionalization according to the physicochemical properties of the surface polymers, and can be used as functionalized nanocomposites, for example, emulsion fixing stabilizers, hydrophilic-hydrophobic conversion materials, catalysts, drug targeting hydrophobic materials and optical biosensors.

The present disclosure relates to an integrated chip including a semiconductor layer and a photodetector disposed along the semiconductor layer. A color filter is over the photodetector. A micro-lens is over the color filter. A dielectric structure comprising one or more dielectric layers is over the micro-lens. A receptor layer is over the dielectric structure. An optical signal enhancement structure is disposed along the dielectric structure and between the receptor layer and the micro-lens.

To solve the problem related to detection limit, specificity and the like of an interference-based optical biosensor.SOLUTION: An optical method for detecting a target molecule (MO) includes: a) forming a sample including MO, in a liquid, into (NP-BR), NP-BR-MO in which the surface of nano particles (NPs) is functionalized with a specific bioreceptor (BR) of the target molecule; b) separating NP-BR-MO and / or NP-BR; c) bringing NP-BR-MO and / or NP-BR into contact with a sensor surface of an optical transducer through reflection and / or transmission, wherein the sensor surface is immobilized and functionalized on (i) MO or (ii) BR or BR1; and d) determining an optical reading value from a change in the interference response of the optical transducer caused by a change of a real portion of the refractive index of the NP conjugate and / or a change in the interference response caused by a result of dispersion or a variation in the complex portion of the refractive index, or both effects.SELECTED DRAWING: Figure 1

The present application relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disrupting activity in sewage and application. The present application creatively designs a Y-shaped polypeptide containing an anchoring region, a hinge region and an anti-fouling region as a structural framework, and performs directional modification at the ends of the main chain and the branch chain to prepare a multifunctional ligand-polypeptide-dye conjugate; a covalent coupling method is used to construct a high-response-efficiency, strong-anti-pollution-characteristic and stable-regeneration optical biosensor chip, i.e. a ligand-polypeptide-dye conjugate functionalized biosensor chip, then a quantum dot-nuclear receptor fluorescence probe with high sensitivity and adjustable emission spectrum is prepared, a ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is proposed, and rapid quantitative detection of endocrine disruptors in sewage is realized, so that the present application has broad-spectrum specific recognition ability for certain endocrine disruptors.

A multi-sensor optical biosensor system to determine one or more health parameters of a user, such as the user's blood pressure. The system includes a multi-sensor wearable device communicatively connected to a user compute device, which may be in communication with a remote compute device. The multi-sensor wearable device could include optical devices to generate a photoplethysmogram (“PPG”) signal that is transmitted to the user compute device. In some cases, the multi-sensor wearable device may be configured to select a PPG channel to transmit to the user's compute device from a plurality of PPG channels based on one or more selection parameters.