10 results about "Optical biosensor" patented technology

The invention is directed to a surface-functionalized optical element comprising an IR-transparent optical element, wherein at least a part of the surface of the IR-transparent optical element is covalently bound to a molecular probe by a linker construct. The linker construct comprises a surface attachment moiety, and a spacer moiety which comprises a poly(alkylene oxide). The invention further relates to a process for preparing said surface-functionalized optical element, to an optical biosensor comprising said surface-functionalized optical element, and to a method for detecting a target analyte which uses said surface-functionalized optical element.

The invention discloses a singularity enhancement effect-based multi-detection optical biosensor, which comprises a microfluidic structure and a sensing chip, the sensing chip comprises a sensing layer, the sensing layer is modified with a specific antibody, the microfluidic structure introduces a to-be-detected liquid into the sensing layer, and the to-be-detected liquid is combined with the specific antibody at the sensing layer, so that the to-be-detected liquid is detected. The sensing layer is realized based on a micro-ring structure of a non-Hermite singular point, when liquid to be detected is not introduced into the sensing layer, the sensing layer is in a second-order singular point state, and when the liquid to be detected is introduced into the sensing layer and is combined with a specific antibody, the second-order singular point state of the sensing layer is broken, a resonance peak in a spectrum is split, and the specific antibody is detected. The relationship between the splitting degree and the concentration of the target substance in the to-be-detected liquid is established through splitting of the harmonic peak, and quantitative detection of the target substance in the to-be-detected liquid is achieved; the method has the advantages that the method does not depend on a high Q value, can also have high sensitivity and low detection limit, can simultaneously cover ultralow concentration detection requirements of a ng / ml level, a pg / ml level and an fg / ml level, and is wider in application scene.

The invention discloses a blood pressure change recognition method and device, wearable equipment and a storage medium. The blood pressure change identification method comprises the following steps: acquiring target sign data acquired by an optical biosensor in a detection time period; performing spectrum analysis on the target sign data to obtain an optical spectrum feature sequence corresponding to the target sign data; according to the target feature mapping relation and the optical spectrum feature sequence, blood pressure change information corresponding to the detection time period is determined; the target feature mapping relation is used for representing a mapping relation between a first optical spectrum feature corresponding to the first sign data collected by the optical biosensor and a pressure spectrum feature corresponding to the pressure data collected by the pressure sensor under the condition that the pressure applying piece applies pressure to the human body. According to the blood pressure change recognition method and device, the wearable equipment and the storage medium, the accuracy of the blood pressure change information determined by the wearable equipment can be improved.

The invention relates to a Bloch surface wave-based high-sensitivity optical biosensor and a detection method thereof. The Bloch surface wave-based high-sensitivity optical biosensor comprises an optical substrate, a multilayer film formed by periodic photonic crystals, a surface functionalized layer and a microfluidic module from bottom to top, by controlling the thickness and refractive index distribution of the multilayer film, the sensor supports a BSW resonance mode at a specific wave band. According to the invention, by constructing a multilayer film formed by periodic photonic crystals, high-Q-value resonance excitation of BSW at 1550nm is realized, and a surface functionalized layer is constructed on the top layer and is used for capturing cancer-related target genes. According to the invention, fine regulation and control of BSW excitation conditions and controllable matching of the interface refractive index are realized.

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

The invention discloses a blood pressure assessment method and device, wearable equipment and a storage medium, and is applied to the wearable equipment which comprises an air pump, an air bag, a pressure sensor and an optical biosensor. The method comprises the steps that an air pump is controlled to adjust the pressure of an air bag, a first physical sign signal of a target user is collected through an optical biosensor, and a pressure signal is collected through a pressure sensor; determining physical sign data according to a first physical sign signal collected by the optical biosensor; determining air bag pressure data according to a pressure signal collected by a pressure sensor; fusing the air bag pressure data and the physical sign data to obtain fused data; a target pressure curve is generated according to the fusion data, the first blood pressure evaluation value is determined according to the target pressure curve, and the reliability and accuracy of blood pressure evaluation can be improved.

Various implementations of a system and method for analyzing extracellular vesicles (EVs) are disclosed having a number of innovative features. In one implementation, a method for analyzing EVs includes binding EVs to an optical waveguide biosensor and phenotyping the bound EVs. Phenotyping can include binding a labeled ligand to the EVs and / or rupturing the EVs and analyzing their cargo. In another implementation, a system for analyzing EVs includes EVs bound to an optical waveguide biosensor and a labeled ligand bound to the EVs. In another implementation, a kit for analyzing EVs includes a micro plate having wells containing optical waveguide biosensors functionalized with a binding agent configured to bind to EVs and at least one of: (a) labeled ligands configured to bind to the extracellular vesicles or (b) a reagent configured to rupture the extracellular vesicles.

The application discloses a modification strategy for inorganic nanoparticles with controllable grafting proportion of multiple ligands on the surface of the inorganic nanoparticles, which can realize the modification of two or more different polymer ligands on the surface of the inorganic nanoparticles according to a specified grafting proportion. The method comprises the following steps: a) synthesizing an initiator with a structure capable of realizing mutual connection, wherein the initiator contains at least one initiating group and at least two mutually reactive groups; b) preparing different kinds of polymers with different molecular weights through atom transfer radical polymerization; c) connecting the different kinds of polymers with different molecular weights according to the designed proportion through the mutually reactive sites on the initiator; and d) modifying the prepared multi-component ligands on the surface of the nanoparticles through ligand exchange or chemical reaction. The modification strategy is suitable for nanoparticles with different sizes and materials and polymer ligands with different kinds and molecular weights. The obtained multi-ligand modified nanoparticles can realize different functionalization according to the physicochemical properties of the surface polymers, and can be used as functionalized nanocomposites, for example, emulsion fixing stabilizers, hydrophilic-hydrophobic conversion materials, catalysts, drug targeting hydrophobic materials and optical biosensors.

The present application relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disrupting activity in sewage and application. The present application creatively designs a Y-shaped polypeptide containing an anchoring region, a hinge region and an anti-fouling region as a structural framework, and performs directional modification at the ends of the main chain and the branch chain to prepare a multifunctional ligand-polypeptide-dye conjugate; a covalent coupling method is used to construct a high-response-efficiency, strong-anti-pollution-characteristic and stable-regeneration optical biosensor chip, i.e. a ligand-polypeptide-dye conjugate functionalized biosensor chip, then a quantum dot-nuclear receptor fluorescence probe with high sensitivity and adjustable emission spectrum is prepared, a ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is proposed, and rapid quantitative detection of endocrine disruptors in sewage is realized, so that the present application has broad-spectrum specific recognition ability for certain endocrine disruptors.