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Electricity-optics joint urine analysis biochemical system and production method thereof

A technology of urine analysis and urine, which is applied in the field of functional materials for biosensors, can solve the problems of poor detection range and limit and other characteristics, and achieve the effects of strong anti-interference ability, good biocompatibility, and improved accuracy

Active Publication Date: 2012-07-18
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Optical biosensors have advantages that electrochemical sensors do not have, such as no reference electrode, good biocompatibility, strong anti-interference ability and high sensitivity, but their detection range and limit are not as good as electrochemical biosensors.

Method used

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  • Electricity-optics joint urine analysis biochemical system and production method thereof
  • Electricity-optics joint urine analysis biochemical system and production method thereof
  • Electricity-optics joint urine analysis biochemical system and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of Porous Alumina Foil Electrode Modified Based on Polypyrrole / Pt Nano-Villi

[0030] 1) Magnetron sputtering a layer of nano-gold on the surface of aluminum oxide foil, and then immersing in 0.1mol / L sulfuric acid solution containing 1mmol / L platinum chloric acid. After the electrode surface is completely black, take it out and repeatedly rinse it with deionized water and acetic acid. Wash to obtain the platinum nano-velvet modified electrode A.

[0031] 2) Add 10 U of enzyme-containing glucose oxidase solution added with appropriate amount of glutaraldehyde and bovine serum albumin dropwise on the surface of platinum nano-hair modified electrode A, and wait for it to dry naturally to prepare an electrical biosensor electrode.

[0032](2) Preparation of optical biosensing film based on fluorescence quenching reaction of bipyridyl ruthenium

[0033] 1) Mix ethyl orthosilicate and deionized water at a molar ratio of 1:2, add an appropriate amount of hydr...

Embodiment 2

[0042] (1) Preparation of Porous Alumina Foil Electrode Modified Based on Polypyrrole / Pt Nano-Villi

[0043] 1) Magnetron sputtering a layer of nano-gold on the surface of aluminum oxide foil, and then immersing it in a 0.8mol / L sulfuric acid solution containing 1mmol / L platinum chloric acid. After the electrode surface is completely black, take it out and repeatedly rinse it with deionized water and acetic acid. Wash to obtain the platinum nano-hair modified electrode A.

[0044] 2) Add 20 U of enzyme-containing glucose oxidase solution added with proper amount of glutaraldehyde and bovine serum albumin dropwise on the surface of platinum nano-hair modified electrode A, and wait for it to dry naturally to prepare an electrical biosensor electrode.

[0045] (2) Preparation of optical biosensing film based on fluorescence quenching reaction of bipyridyl ruthenium

[0046] 1) Mix ethyl orthosilicate and deionized water at a molar ratio of 1:4, add an appropriate amount of hydro...

Embodiment 3

[0051] (1) Preparation of Porous Alumina Foil Electrode Modified Based on Polypyrrole / Pt Nano-Villi

[0052] 1) Magnetron sputtering a layer of nano-gold on the surface of aluminum oxide foil, and then immersing it in a 0.8mol / L sulfuric acid solution containing 1mmol / L platinum chloric acid. After the electrode surface is completely black, take it out and repeatedly rinse it with deionized water and acetic acid. Wash to obtain the platinum nano-hair modified electrode A.

[0053] 2) Add 50 U of enzyme-containing glucose oxidase solution added with proper amount of glutaraldehyde and bovine serum albumin dropwise on the surface of platinum nano-hair modified electrode A, and wait for it to dry naturally to prepare an electrical biosensor electrode.

[0054] (2) Preparation of optical biosensing film based on fluorescence quenching reaction of bipyridyl ruthenium

[0055] 1) Mix ethyl orthosilicate and deionized water at a molar ratio of 1:5, add an appropriate amount of hydro...

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Abstract

An electricity-optics joint urine analysis biochemical system and a production method thereof relate to functional materials for biosensors. The system is provided with a urine reaction cell, an LED (light-emitting diode) light source, an optical fiber spectrometer, a singlechip and a display circuit; an optical biosensor, an electrochemical biosensor and a pH (Potential Of Hydrogen) meter are arranged in the reaction cell, the upper end of the reaction cell is provided with a urine inlet, the bottom of the reaction cell is provided with a urine outlet, and a modified electrode and a reference electrode of the electrochemical biosensor are arranged on a lower protective jacket end of the urine reaction cell; the output end of the optical biosensor is connected with the input end of the optical fiber spectrometer, the output end of the optical fiber spectrometer is connected with the singlechip, the output end of the electrochemical biosensor is connected with the singlechip, and the output end of the pH meter is connected with the singlechip; and the singlechip is connected with the display circuit. The polypyrrole / platinum nano-hair-based modified porous alumina foil electrode is first prepared, the bipyridyl ruthenium fluorescence quenching reaction-based optical biosensor is then prepared, and finally, the electricity-optics joint urine analysis biochemical system is constructed.

Description

technical field [0001] The invention relates to functional materials for biosensors, in particular to an electrical and optical urine analysis biochemical device and a manufacturing method thereof. Background technique [0002] Biosensor technology is a very active field of engineering technology research developed in recent years. Its characteristics of high automation, miniaturization and integration reduce the requirements for user environment and technology. Provides a fast and simple new method, which can be widely used in the detection of various biochemical indicators such as trace proteins in body fluids, small molecular organic substances (such as glucose, antibiotics, amino acids, cholesterol and lactic acid, etc. in the body), nucleic acids, etc. , has become a research hotspot. In 1967, Updik and Hicks first developed the glucose oxidase (GOD) electrode with a platinum electrode as the substrate for quantitative detection of glucose content in serum (S.J.Updike,...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N27/26G01N27/327
Inventor 熊兆贤陈淑娴林丞薛昊邱虹
Owner XIAMEN UNIV
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