273 results about "Surface plasmonic resonance" patented technology

The invention relates to the field of super-hydrophobic materials, in particular to a basalt fiber composite material as well as a preparation method and application thereof. According to the composite fabric material provided by the invention, a multistage micro-nano rough surface of the MoS2 nanoflowers and the silver nanoparticles is constructed on the fiber substrate, so that not only is super-hydrophobic self-cleaning realized, but also the anti-icing performance is relatively high, and particularly, rapid heating is realized by combining broadband absorption of the MoS2 nanoflowers and a local surface plasma resonance effect of the silver nanoparticles; and meanwhile, the silver nanoparticles accelerate heat conduction, so that excellent ice melting capability is obtained. The high conductivity of the silver nanoparticles constructs a conductive path, and the layered structure of MoS2 triggers interface polarization, so that the electromagnetic shielding effectiveness of the X wave band is improved. The dual antibacterial mechanism of the MoS2 nanoflowers and the silver nanoparticles also realizes broad-spectrum efficient antibiosis, the preparation method is green and environment-friendly, the cost is low, and the requirements of complex scenes such as wearable electronic equipment, medical protection, polar region protection and national defense can be met.

The invention belongs to the technical field of terahertz detection, and discloses a terahertz metamaterial enhanced spectrum detection system. Comprising the following steps: constructing a metamaterial enhancement factor tensor matrix according to the interaction between terahertz waves and a metamaterial; performing local field enhancement and surface plasma resonance optimization on the target sample based on the metamaterial enhancement factor tensor matrix to obtain a feature enhancement spectroscopy mapping function; constructing a multi-dimensional detection feature space based on a feature enhancement spectroscopy mapping function; a spectral signal enhancement topology network is formed based on the multi-dimensional detection feature space; enhancing the topological network based on the spectral signal, and generating an enhanced spectral feature discrimination function; obtaining a high-precision terahertz spectrum detection result based on the enhanced spectrum characteristic discrimination function; according to the invention, internal correlation between spectral characteristics can be mined, and comprehensive description of sample information is enhanced, so that high-precision terahertz spectrum detection is realized.

The invention discloses a single-groove type photonic crystal fiber sensor based on surface plasma resonance. The single-groove type photonic crystal fiber sensor structurally comprises a fiber core and a perfect matching layer wrapping the fiber core, and circular air holes which are arranged periodically and are different in size are formed in the fiber core; the fiber core is provided with a groove channel, and the lower surface of the groove channel is coated with a gold film and a titanium dioxide film. A loss spectrum is obtained through calculation, and then the wavelength of the surface plasma formant and the sensitivity of the sensor are calculated. The refractive indexes of the to-be-detected analysis liquid sample are different, and the wavelengths of the resonance peaks of the transmission loss spectrum are also different. The refractive index range of a to-be-detected analysis liquid sample of the sensor is 1.39-1.4, the sensitivity of 11000 nm / RIU and the resolution of 9.09 * 10 <-6 > RIU can be obtained, the sensitivity and the resolution of the sensor are superior to those of an existing sensor, and the sensor is simple in structure and easy to operate and has great application prospects in the fields of biosensing, environmental monitoring and the like.

Disclosed herein is an anti-transferrin receptor antibody that specifically binds to the human transferrin receptor and the rhesus monkey transferrin receptor, comprising i) a humanized heavy chain variable domain derived from the heavy chain variable domain of SEQ ID NO:01, and ii) a humanized light chain variable domain derived from the light chain variable domain of SEQ ID NO:26, wherein the dissociation rate of the antibody for the human transferrin receptor is equal to or less than (i.e., at most) the dissociation rate of anti-transferrin receptor antibody 128.1 for the rhesus monkey transferrin receptor, wherein the dissociation rate is determined by surface plasmon resonance, and anti-transferrin receptor antibody 128.1 has a heavy chain variable domain of SEQ ID NO:64 and a light chain variable domain of SEQ ID NO:65.

The invention discloses a hybrid Ag / MXene / Fe-Co MOF surface enhanced Raman scattering sensor and an application of the hybrid Ag / MXene / Fe-Co MOF surface enhanced Raman scattering sensor in rapid detection of bacteria. According to the sensor, silver nanoparticles are prepared through a sodium citrate reduction method, a two-dimensional material MXene and a bimetal organic framework are combined, and a surface enhanced Raman scattering spectrum detection platform with high sensitivity, stability and anti-interference performance is constructed. The conductive network of MXene and the local surface plasmon resonance effect of silver nanoparticles synergistically enhance an electromagnetic field, and the porous structure of Fe-Co MOF enriches target molecules and forms dense hot spots, so that the Raman signal intensity is remarkably improved. Experiments show that the detection limit of the sensor to 4-mercaptobenzoic acid is as low as 10 <-12 > M, and the signal stability can last for 7 days; the detection limit on common pathogenic bacteria such as escherichia coli and staphylococcus aureus reaches 10 CFU / mL, and the linear correlation coefficient is superior to 0.96. The sensor has hydrophobicity and biocompatibility, can efficiently capture bacteria through capillary force, is suitable for trace detection of complex samples, and provides a new strategy for rapid diagnosis of clinical drug-resistant bacteria.

The invention discloses a surface plasmon resonance imaging system for expanding a dynamic range through a multi-mode optical path, and relates to the technical field of microscopic imaging, a laser outputs a monochromatic and linearly polarized continuous wave beam, a first dynamic beam deflection device performs dynamic angle deflection adjustment on the continuous wave beam, a prism is used for providing a total reflection interface, and a second dynamic beam deflection device is used for performing dynamic angle deflection adjustment on the continuous wave beam. Exciting an evanescent wave; a surface plasma resonance effect is generated through interaction of the SPR detection chip and evanescent waves, and sample characteristics are detected; the flow cell provides a sample flow channel, so that a sample is in full contact with the surface of the SPR detection chip; the second dynamic light beam deflection device cooperatively adjusts the propagation direction of reflected light on the surface of the SPR detection chip, and the imaging detection device captures a high-spatial-resolution SPR image formed by the reflected light and transmits the image to the upper computer in real time for processing and analysis. According to the invention, the change of the resonance angle can be tracked in real time, and high-sensitivity and high-resolution monitoring of the dynamic process of biomolecule interaction is realized.

The invention relates to the technical field of biosensing, and discloses an ultra-sensitive biosensor as well as a preparation method and application thereof. The ultra-sensitive biosensor comprises a substrate, a two-dimensional material loaded on the substrate, DNA origami loaded on the two-dimensional material and quantum dots modified on the DNA origami, wherein the DNA origami contains an exposed single-stranded DNA fragment, and the single-stranded DNA fragment can be cut by an activated Cas protein; the quantum dots are connected with the tail ends of the single-stranded DNA fragments. The ultrasensitive biosensor is excellent in performance, and compared with a conventional pure optical biosensor combining surface plasma resonance and DNA origami, the sensitivity of the ultrasensitive biosensor is improved by four orders of magnitude and can reach the aM level.

The invention discloses a high-sensitivity SPR refractive index sensor with a sawtooth structure and a graphene-silver layer, and belongs to the technical field of sensors. A smooth prism surface is etched into a sawtooth shape, metal silver-two-dimensional nano material graphene is used as a plasma material to form silver layer-graphene, the plasma material contains silver, a silver film is covered with graphene, and the prism is etched into a sawtooth shape by optimizing the thickness of a sawtooth silver layer and the number of layers of black phosphorus. The thickness of a silver layer, the height of sawteeth and the number of layers of graphene are adjusted through the composite film plated with silver-graphene, and the prism with the sawteeth structure and the metal and two-dimensional nanometer material are provided. The average angle sensitivity of the sensor disclosed by the invention can reach 860.6 degrees / RIU in a refractive index range from 1.330 RIU to 1.335 RIU. Compared with a prism type surface plasmon resonance refractive index sensor reported in the past, the surface plasmon resonance refractive index sensor has higher angle sensitivity.

The invention discloses a preparation method and application of a peptide microtube / SnO2 / Au composite gas-sensitive material. The three-dimensional network structure of the peptide microtube is utilized to effectively adsorb SnO2 and Au nanoparticles, so that the SnO2 and Au nanoparticles form a complete connection path on the surface of the peptide microtube, and electron transfer is facilitated; and gas diffusion is also facilitated. The localized surface plasmon resonance (LSPR) effect of Au nanoparticles can enhance visible light absorption and surface reaction activity, so that the gas-sensitive property is improved, and rapid adsorption and desorption reaction can be carried out at room temperature. The prepared sensor exerts the synergistic effect of the three materials, realizes efficient detection of NO2 at room temperature, has very high selectivity to NO2, and has relatively high response to low-concentration NO2, so that trace detection can be carried out.

The invention belongs to the technical field of SERS (Surface Enhanced Raman Scattering) detection, and relates to a preparation method of an Ag-Fe3O4 composite SERS substrate and application of the Ag-Fe3O4 composite SERS substrate in monitoring trace pollutants in water. Based on a plasma-semiconductor synergistic enhancement mechanism, cubic Fe3O4 nano particles with exposed {100} crystal faces are synthesized through a hydrothermal method, and the cubic Fe3O4 nano particles and Ag nano particles are subjected to ultrasonic-stirring to construct the Schottky heterojunction. The substrate creatively realizes dual-channel enhancement: the local surface plasmon resonance effect of Ag nanoparticles generates electromagnetic enhancement, the narrow band gap of Fe3O4 promotes photo-induced electrons to be transferred to Ag, an interface electric field is formed, and the polarizability of adsorption molecules is remarkably increased. When the probe is applied to detection of trace pollutants in a water body, the probe molecule RB is anchored on the surface of Fe3O4 through carboxyl, and the HOMO energy level of the probe molecule RB and the valence band top of Fe3O4 generate resonance charge transfer, so that the Raman cross section is improved. The detection limit of RB under 0.25 mW laser is increased by several orders of magnitude compared with that of a single Ag substrate.

The invention provides a heterogeneous dual-core optical fiber SPR (Surface Plasmon Resonance) microfluidic chip system for biochemical detection. The system is characterized by comprising a heterogeneous dual-core optical fiber 1, a dual-core optical fiber movable connector 2, a dual-core optical fiber SPR probe 3, a microfluidic chip 4, a dual-core optical fiber fan-in and fan-out device 5, a double-clad optical fiber 6, a large-core-diameter optical fiber 7, a spectrograph 8, a light source 9 and the like, wherein the double-core optical fiber 1 comprises a middle single-mode fiber core and a biased multi-mode fiber core, the end face of the single-mode fiber core is subjected to inclined grinding and plated with a gold film for exciting the surface plasma resonance effect, the end face of the multi-mode fiber core collects SPR reflected light signals through a secondary reflection inclined plane, and the double-core optical fiber SPR probe 3 is embedded into the micro-flow chip 4. The device has the advantages of high sensitivity, low loss and multi-channel detection, and can be widely applied to the fields of biochemical detection, environmental monitoring, precision medical treatment and the like.

The invention provides an optical fiber biosensor, a preparation method thereof and an optical fiber sensing system.The optical fiber biosensor comprises an optical fiber microstructure with an optical fiber sensing part and a surface plasma resonance layer, and the outer surface of the optical fiber sensing part is coated with the surface plasma resonance layer; the surface plasma resonance layer is used for exciting the optical fiber sensing part to generate surface plasma waves and combining or adsorbing a sensing target, and the surface plasma resonance layer is an AuNPs (at) MOF composite film; the AuNPs (at) MOF composite film is combined on the outer surface of the optical fiber sensing part through a chemical modification method, the optical fiber sensing part can be excited to generate surface plasma waves, and a sensing target is combined or adsorbed, so that a sensing film corresponding to a target detection object does not need to be coated during follow-up biological sensing, and the cost is reduced. And the manufacturing cost of the optical fiber biosensor based on the LSPR effect is further reduced.

The invention relates to the field of optical fiber sensing, in particular to a multichannel optical fiber surface plasma resonance sensing probe as well as a preparation method and application thereof. According to the multichannel optical fiber surface plasma resonance sensing probe, the sensing probe adopts an end face reflection type optical fiber sensing structure, and the end face reflection type structure is formed by welding a multimode optical fiber and a coreless optical fiber. A multimode-coreless optical fiber welded end face reflection type structure is adopted, SPR signals are excited through end face total reflection, the defect that a traditional lateral coupling type optical fiber SPR sensor is difficult to carry out directional detection in narrow spaces (such as blood vessels and tissue gaps) is overcome, miniaturized insertion type diagnosis with the minimum diameter being 300 micrometers is achieved, and the SPR sensor is suitable for in-situ monitoring of living tissue; according to the invention, through the nanocluster modification layers (0 / 2 / 4 layers) which are axially distributed at intervals, multi-channel functionalization can be completed only by a pulling method, traditional micro-nano processing and a complex film layer structure are not needed, and the preparation time of the multi-channel sensor is shortened from gt to gt; and the time is shortened from 6 hours to 1 hour.

A surface plasmon resonance sensor system includes a sensor plate having a quartz glass substrate and a copper film sputtered onto the substrate and annealed for about two hours. A quartz prism is attached to a surface of the sensor plate, and a microfluidics unit containing carrier fluid is attached to the opposite surface. A target analyte reservoir is connected through a pump to inject target analytes into the microfluidics unit. A binder is attached to the copper film and binds to target analytes. A laser transmits a beam into the quartz prism at an incident angle that generates surface plasmons resonating with the copper film and reflects a phase shifted laser beam. Receive optics and a charge coupled device convert the phase shifted beam to electrical signals. A computing unit processes the electrical signals to compute refractive index changes based on phase shifts to indicate target analyte detection.

The invention provides a multicolor visual semi-quantitative detection method for miRNA in skin interstitial fluid, which comprises the following steps: extracting a skin interstitial fluid sample in a minimally invasive manner by combining a microneedle patch with a vacuum negative pressure technology; the method comprises the following steps: incubating a skin interstitial fluid sample and a magnetic nanoprobe of which the surface is immobilized with a hairpin probe H1, opening H1 in the presence of target miRNA, triggering a subsequent hybridization chain reaction of a hairpin probe H2 and H3, and constructing a biotin-rich DNA compound on the surface of a magnetic bead; the method comprises the following steps: carrying out enzyme labeling by using an alkaline phosphatase-streptavidin conjugate, catalyzing ascorbic acid phosphate to generate ascorbic acid to reduce potassium iodate, and generating an iodine elementary substance to etch a gold nano bipyramid, so that the color of a solution is changed, and high-sensitivity detection on target miRNA is realized. The multi-color visual detection method provided by the invention is combined with the gold nano bipyramid localized surface plasmon resonance property change, so that near-full spectrum color change can be generated, and the portability of the sensor and the accuracy of visual analysis can be improved.

The invention discloses a preparation method of an MF (at) NH2 (at) Ag SERS (Surface Enhanced Raman Scattering) sensor for detecting paraquat and diquat, belongs to the field of nano material synthesis and spectral analysis, and solves the problems that a traditional detection method is complicated in process and cannot detect pesticides and free radicals thereof at the same time, and a traditional SERS substrate is poor in enhancement effect and low in stability. The preparation method comprises the following steps: firstly segmenting melamine foam, cleaning and drying, soaking in hydrochloric acid, soaking in a polyethyleneimine aqueous solution to modify amino, drying, soaking in a silver nitrate solution for ultrasonic treatment, adding a sodium borohydride solution under an ice bath, stirring and reducing, and finally cleaning and drying to obtain the sensor. The sensor takes three-dimensional melamine foam as a support, silver nanoparticles are uniformly loaded on the surface of the three-dimensional melamine foam, paraquat, aquacide and free radicals thereof are enriched through electrostatic interaction based on a localized surface plasma resonance principle, and Raman signal enhancement is realized. The detection limit is 10 <-9 > mol / L, the signal stability and repeatability are excellent, the applicability to actual water sample detection is good, the detection of other pesticides can be expanded, and the method is suitable for on-site rapid trace detection.

Provided are compounds, formulations comprising compounds, and devices that utilize compounds, where the devices include a substrate, a first electrode, an organic emissive layer comprising an organic emissive material disposed over the first electrode. The device includes an enhancement layer, comprising a plasmonic material exhibiting surface plasmon resonance that non-radiatively couples to the organic emissive material and transfers excited state energy from the organic emissive material to the non-radiative mode of surface plasmon polaritons. The enhancement layer is provided no more than a threshold distance away from the organic emissive layer, where the organic emissive material has a total non-radiative decay rate constant and a total radiative decay rate constant due to the presence of the enhancement layer. At least one of the organic emissive material and the organic emissive layer has a vertical dipole ratio (VDR) value of equal or greater than 0.33.

A method for manufacturing a surface-enhanced Raman scattering substrate for detecting virus macromolecules, in which a high-refractive spherical open cavity array with uniform noble metal nanoparticles on the inner surface is manufactured by using a dielectric nanoparticle monolayer close-packed array as a template, the coupling effect of surface plasmon resonance of noble metal particles and high-order magnetic resonance of high-refractive spherical cavities is utilized to form a uniform electric field enhanced hot zone on the inner surface of the cavity; after virus molecules enter the high-refractive spherical cavity with adsorbed noble metal particles, the virus molecule surface directly contacts the noble metal particles adsorbed on the inner surface of the high-refractive spherical cavity in a large area, that is, the virus molecules, the noble metal particles and the inner surface of the spherical cavity are coupled to overlap the electric field hot zone, which greatly improves the Raman signal intensity of the virus molecules and realizes high-sensitivity detection of the virus molecules.

To provide a hand-held or portable SPR device.SOLUTION: A hand-held or portable SPR device focuses light at two or more positions on a noble metal sensor surface, functionalized to enable detection of different SPR curves and their respective minima. The detection is accomplished substantially simultaneously by obtaining SPR signals contemporaneously from the different locations on the sensor surface, on the same sensor. The SPR device may incorporate multiple light sources or a single light source with a beam that is shifted by deflectors in seriatim to focus on different positions on along the functionalized sensor surface. To control wettability of the sensor surface (to augment multiplexing), the sensor surface may include i) the addition of surface assembled monolayers (SAMs) and ii) surface bombardment by gaseous plasmas, most notably O2.SELECTED DRAWING: Figure 1A

This invention discloses a copper-tungsten oxide / zinc oxide heterojunction photocatalyst, its preparation method, and its application, belonging to the field of nanomaterials and photocatalysis technology. Firstly, a single-atom copper-modified plasma W-type photocatalyst is synthesized using a solution-thermal method combined with an impregnation-thermal reduction process. 18 O 49 Nanowires were first prepared; then porous ZnO nanosheets were prepared via a hydrothermal method combined with calcination; finally, single-atom copper-modified plasma W was constructed via electrostatic self-assembly. 18 O 49 Nanowire / porous ZnO nanosheet heterojunction composite material. This invention utilizes the above-described preparation method to prepare Cu-W nanowires with matched band structures. 18 O 49 / ZnO heterojunction can realize the photogenerated electrons of ZnO into the plasma Cu-W 18 O 49 Nanowire implantation stabilizes and increases the surface oxygen vacancy concentration and free electron density, thereby enhancing the surface plasmon resonance effect and promoting the continuous generation of high-energy hot electrons. Single-atom copper active sites and W 18 O 49 The synergistic effect of nanowires enables the enriched hot carriers to efficiently and selectively photocatalytically reduce CO2 to CH4.

The present invention discloses a method for preparing sunscreen fibers with aluminum and cerium co-doped zinc oxide quantum dots. By utilizing the local surface plasmon resonance of metal oxides to enhance the emissivity of the polymer substrate, a relatively high radiation cooling power is obtained. At the same time, the co-doped zinc oxide quantum dots have a relatively high ultraviolet absorption rate. Through a special shear oscillation melt extrusion process, the uniform dispersion of zinc oxide in nylon fibers is achieved. When the prepared fibers are irradiated by sunlight, the measured value of UPF is greater than 50, and the transmittance of UVA is less than 5%, meeting the requirements of ultraviolet protection products. In addition, when irradiated by the sun, the maximum temperature difference between the inside and outside of the zinc oxide nylon fibers reaches 4°C, showing an obvious cooling effect. The present invention has wide applications in sunscreen clothing.

The application provides a skin interstitial fluid miRNA multicolor visualization semi-quantitative detection method, which comprises the following steps: incubating a skin interstitial fluid sample with magnetic nano probes of surface immobilized hairpin probes H1, opening H1 and triggering a subsequent hybridization chain reaction of hairpin probes H2 and H3 in the presence of target miRNA, and constructing a biotin-rich DNA complex on the surface of the magnetic beads; performing enzyme labeling by using an alkaline phosphatase-streptavidin coupling compound, and catalyzing ascorbic acid phosphate to generate ascorbic acid potassium iodate, so that the color of the solution changes, and high-sensitivity detection of the target miRNA is realized. The multicolor visualization detection method can produce near-full-spectrum color changes by combining the gold nano double-cone local surface plasmon resonance property change, and is beneficial to improving the portability of the sensor and the accuracy of the visual analysis.

The invention relates to a photochromic lens with a photochromic dye grafted nanoparticle composite film layer and a preparation method of the photochromic lens, and belongs to the technical field of optical lenses. The photochromic lens comprises a lens base material, a priming coat, a photochromic dye grafted nanoparticle composite film layer and a protective layer which are sequentially stacked from bottom to top, wherein chemical bond grafting is formed between the photochromic dye and the surfaces of the gold / silver nanoparticles through sulfydryl or carboxyl at the tail end of a molecule of the photochromic dye. The localized surface plasmon resonance effect of the covalent grafting structure and the nanoparticles synergistically solves the bottlenecks of easy agglomeration, low loading capacity and slow response of the dye in the traditional technology. The lens disclosed by the invention has the characteristics of extremely fast color-changing response, high color fading efficiency, high mechanical strength, good environmental stability and the like, and the comprehensive performance and the service life of the color-changing lens are remarkably improved and prolonged.

The invention belongs to the technical field of energy materials, and discloses a photo-assisted rechargeable lithium ion battery based on a Schottky heterostructure positive electrode, the photo-assisted rechargeable lithium ion battery comprises a lithium negative electrode, a solid electrolyte, a Schottky heterostructure photo-assisted positive electrode and FTO conductive glass with a transparent substrate, and a titanium dioxide semiconductor material and metal gold are compounded to serve as the battery positive electrode. On one hand, gold is nanoparticles, a plasma effect exists, and the light absorption capacity is remarkably improved, on the other hand, a built-in electric field is generated at a phase interface, the material has a lower ion migration energy barrier in the electrochemical process, and the ion diffusion capacity is higher and faster; the positive electrode material not only can collect solar energy similar to a solar cell, but also can store solar energy like energy storage equipment, so that the complexity and energy loss of a solar system are reduced; meanwhile, through the plasma effect, surface plasma resonance is shown, the optical path is increased, and a built-in electric field of the heterostructure achieves effective separation of photo-induced electrons and holes, so that the battery interface resistance is effectively reduced, the conductivity of an electrode material is increased, and the battery capacity is improved.

The invention relates to the technical field of optical sensing detection, in particular to an intensity modulation sensing method with an optional excitation wavelength and aluminum as a surface plasma resonance material. According to the linear relation between the excitation wavelength and the optimal thickness of the aluminum film, the optimal thickness of the aluminum film with the selected excitation wavelength is obtained; preparing an aluminum film with the corresponding optimal thickness according to a thermal evaporation technology; determining a reflection angle spectrum of the aluminum film through a surface plasma resonance detection system, and calibrating a fixed incident angle of intensity modulation; the medium environment contacted with the aluminum film is changed, the reflection intensity is detected in real time, and the refractive index of the medium environment is obtained according to the linear relation between the refractive index and the reflection light intensity. According to the invention, aluminum is used as a surface plasma resonance material, surface plasma resonance from deep ultraviolet to near infrared is realized, and random selection of excitation light wavelength in the wavelength range is realized by utilizing the approximate linear characteristics of a real part and an imaginary part of the refractive index in the range of 400-700nm.

To achieve high spectral performance [Solution] The infrared spectrometer according to the embodiment comprises a diffraction grating structure, a metal thin film, and a current detection unit. The diffraction grating structure includes continuous convex and concave portions. A metal thin film is formed on the surface of the diffraction grating structure so that surface plasmon resonance can be excited when infrared light is incident at a specific incident angle. The current detection unit detects the current generated by the surface plasmon resonance. The pitch of the uneven structure in the diffraction grating structure is 1.52 times or more and 2.17 times or less the width of the convex portion.

An aerosol-generating device is disclosed. The aerosol-generating device includes a storage chamber storing an aerosol-generating substance, a wick that is in communication with the storage chamber, a heating element that is in contact with the wick and contains nanoparticles generating heat through surface plasmon resonance, and a light source configured to emit light toward the heating element. The heating element includes a first part receiving light emitted from the light source and a second part disposed outside the first part and having a plurality of holes formed therein.

An surface plasmon microscope includes a light source, a polarization element, a beam splitter, an objective lens, a photodetector, an operation unit, and the like. An illumination optical system including lenses, the polarization element, the beam splitter, and the objective lens guides light output from the light source to converge the light by the objective lens, and illuminates a metal thin film with the light from a lower surface side of a transparent substrate and focuses the light on the metal thin film to generate a surface plasmon resonance. A detection optical system including the objective lens, the beam splitter, and a lens guides reflected light generated by the focused illumination on the metal thin film to the photodetector. The operation unit acquires refractive index information of a sample disposed in contact with the metal thin film based on the reflected light intensity detected by the photodetector.

The application discloses a wavelength modulation surface plasmon resonance imaging sensing method, which generates multiple groups of illumination spectra by controlling a light-emitting diode, processes the spectra into p-polarized light, excites a sensing chip to generate surface plasmon resonance through prism coupling, collects corresponding reflected light intensity images by using a CMOS camera, and reconstructs surface plasmon resonance reflection spectra of each pixel on the sensing chip by using a smooth constraint reconstruction algorithm according to intensity observation values of the pixel in all images and corresponding illumination spectrum data, so as to further extract a phase signal and generate a spatially resolved phase image. The phase signal and the imaging scheme realize high-sensitivity and high-throughput sensing performance. The method realizes low-cost, high-signal-to-noise ratio and high-throughput sensing imaging.

The invention provides a photoelectrochemical aptamer sensor for detecting alpha fetoprotein and a preparation method of the photoelectrochemical aptamer sensor, and relates to the technical field of sensors. According to the invention, a solvothermal method and a chemical reduction method are combined to gradually load BiPO4 nanosheets and Ag NPs onto TiO2NRA, so that the Ag / BiPO4 / TiO2NRA ternary composite material is obtained. Through the synergistic effect of a Type II type n-n heterojunction formed by TiO2NRA and BiPO4 and the surface plasma resonance (SPR) effect of Ag, the utilization rate of a photoelectric material to visible light is increased, and the photoelectric property of the photoelectric material is improved, so that the sensitivity of a photoelectrochemical biosensor is improved, and the detection range of alpha fetoprotein to be detected is expanded. In addition, a Type II type n-n heterojunction constructed by BiPO4 and TiO2NRA improves the transfer rate of electrons, the SPR effect of metal Ag is combined with the Type II type n-n heterojunction structure, a visible light source is utilized to a great extent, the photoelectric property of the photoelectrochemical aptamer sensor is improved, the sensitivity of the alpha fetoprotein photoelectrochemical aptamer sensor is further improved, and the detection limit is lower.