Lead ion colorimetric detection probes and application method thereof

A technology for detecting probes and colorimetric probes, which is applied in the field of detection, can solve the problems of high cost of DNA and enzymes and cannot be the choice of general application, and achieve the effect of simple method, easy operation and good selectivity

Inactive Publication Date: 2010-05-19
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, nucleic acid aptamers (adapters) or DNA enzymes have been used as functional reagents to prepare probes with gold nanoparticles for the detection of Pb 2+ and other heavy metal ions [Wang Z D, Lee J H, Lu Y, Adv. Mater.2008, 20, 3263-3267; Liu J W, Lu Y, 2004, J. Am. Chem. So

Method used

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  • Lead ion colorimetric detection probes and application method thereof
  • Lead ion colorimetric detection probes and application method thereof
  • Lead ion colorimetric detection probes and application method thereof

Examples

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Embodiment 1

[0026] At room temperature, the aqueous solution of chloroauric acid and glutathione are mixed and stirred to form an aqueous solution according to the mass ratio of 6:1, and the pH value of the mixed solution is adjusted to 7.0 with 1M sodium hydroxide solution, and then in the mixed solution Add the newly prepared sodium borohydride ice water solution (2mg / 1mL) and stir vigorously. The mixed solution is continuously reacted for 12 hours under stirring conditions, centrifuged to remove unreacted substances and redispersed to obtain glutathione functionalized gold Nanoparticles, that is, colorimetric probes, measure their UV-Vis absorption spectrum, and have characteristic absorption at 520nm.

Embodiment 2

[0028] When using a colorimetric probe to detect lead ions, dilute the prepared colorimetric probe 4 times, take 100 μl of the diluted colorimetric probe solution and add it to the same amount of biological tissue or environmental sewage sample to be tested, and then add 20 μl , 1M NaCl solution, judge whether there are lead ions by observing the color change of the solution. When lead ions exist, the solution changes from the red color of gold particles to purple or blue-gray; or test the ultraviolet-visible absorption spectrum of the solution, and the presence of lead ions , the ultraviolet-visible characteristic absorption spectrum of the solution is red-shifted, and the absorption spectrum has a good linear relationship with the concentration of lead ions, so as to judge whether there are lead ions in the sample to be tested.

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Abstract

The invention belongs to the technical field of detection and particularly relates to lead ion colorimetric detection probes and an application method thereof. In the method, glutathione modified gold nanoparticles are used as colorimetric probes, glutathione combines with gold nanoparticles by utilizing sulfydryl groups of the glutathione for coordination to form a stable structure of which the surface contains two free carboxyl radicals. In the process of detection, the carboxyl radicals can coordinate with heavy metal ions; and by utilizing the property of surface plasma resonance of the gold nanoparticles, the existence of the heavy metal ion Pb2+ in aqueous solution can be indicated by the color change of the solution. The lead ion colorimetric detection probes have the advantages that: the sensitivity and selectivity are high; in-situ quick detection can be realized without large-size instruments; the detection results are visual and can be observed by naked eyes; the operation is simple and the cost is low; and the used solvents and the operation processes have no toxic or side effects.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a lead ion colorimetric detection probe and an application method thereof. Background technique [0002] Heavy metal ions (mercury Hg 2+ ; lead Pb 2+ ; Cadmium Cd 2+ ; Copper Cu 2+ ), especially mercury, cadmium, lead, copper, etc. have significant biological toxicity. They cannot be degraded by microorganisms in the water body, but can only undergo the process of mutual transformation, dispersion and enrichment of various forms (ie migration). Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and use of heavy metal products. The degree of harm depends on the concentration and chemical form of heavy metals in the environment, food and organisms. [0003] Heavy metals generally exist in nature in natural concentrations,...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/33
Inventor 关威苏忠民柴芳代起君王春刚李鹿赵亮高洪泽
Owner NORTHEAST NORMAL UNIVERSITY
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