287results about How to "Detection is simple and fast" patented technology

Nucleic acids are fixed or immobilized to non-porous solid supports (substrates), and include systems containing such supports and arrays with fixed or immobilized nucleic acids. These compositions are useful for nucleic acid analyses and a host of applications, including, for example, detection, mutational analysis and quantification. The non-porous solid supports can be transparent or translucent, and the surfaces can be treated with agents to fix or immobilize the nucleic acids. Such agents include, for example, amine providing compounds, epoxy compounds and acid solutions. The fixed or immobilized nucleic acids can be unlabeled, or labeled with at least one non-radioactive signaling moiety, such as the case when the nucleic acids are double-stranded.

The present invention relates to an arrangement comprising an Ethernet carrier network, managed by a management system (100) and comprising a number of levels of management domains, each comprising a number of edge nodes (10, 20, 30, 40) and a number of intermediate nodes (1, 2, 3, 4, 5, 6, 7, 8). Each edge node (10, 20, 30, 40) comprises fault detection of connectivity check messages and fault locating means with holding means (11, 21, 31, 41) adapted to hold path information for the paths to all other edge nodes. A fault detection means of an edge node having detected a fault is adapted to activate edge node fault locating means to locate the fault using the patch information in the edge node holding means to find a first target intermediate node substantially in the middle of the path containing the fault, and to, directly, send a first loop back message to said intermediate node. A response from said intermediate node indicates that the fault is located at the distant portion of the path, no response indicates that the fault is located at the proximate portion of the path. Further loop back messages are sent to consecutively identified intermediate nodes of the respective path portion identified to contain the fault by the respective preceding loop back message until the fault is localized.

A method and means for delivery of drugs to the chorio-retina and the optic nerve head which comprises contacting the surface of the eye with an effective amount of drug for treatment of chorio-retina and optic nerve head and a physiologically acceptable adrenergic agent for enhancing delivery of the drug to these tissues in an ophtalmologically acceptable carrier, said adrenergic agent being selected from the group consisting of alpha adrenergic agonist agents, derivatives of the alpha adrenergic agonist agents, beta-blocking agents, derivatives of the beta-blocking agents and mixtures thereof.

The invention relates to a steering engine tester and a testing method thereof, which belong to the technical field of guided bombs. The tester comprises a control cabinet and a simulation load table, wherein the control cabinet comprises a steering engine controller, a regulated power supply, an industrial control computer, a display, a keyboard and a main power supply switch; the simulation load table comprises a photoelectric encoder, a torque sensor and a magnetic powder brake; a steering engine shaft of a steering engine to be tested is mechanically connected with a rotating shaft of the photoelectric encoder through a shaft coupling; the steering engine to be tested is in digital signal communication with the steering engine controller through a data line; and steering engine testing software is installed in the industrial control computer. The tester provided by the invention has the advantages of more plentiful testing contents, more complete testing equipment, more intelligent testing software, prompting of operating information on a software interface, shielding of keys which are required to be operated, change of parameter input items according to selected testing items, information prompt for improper operation, and great reduction in the probability of error operation.

The invention discloses a method for recognizing a zebra crossing and measuring the zebra crossing distance based on line-by-line scanning. The method for recognizing a zebra crossing comprises the following steps: constructing a wavelet function template containing an odd wavelet function and an even wavelet function; carrying out line-by-line scanning on an image to be detected, and respectively carrying out convolution operation on grey scale value vectors of all pixel points in each scanning line with the odd wavelet function and the even wavelet function; defining a response function formula, and calculating response function vectors corresponding to all the pixel points in each scanning line in accordance with the formula; and recognizing the zebra crossing in accordance with response function values and a preset threshold. After the zebra crossing is recognized, by calibrating the distance between an imaging device and the image formed by the imaging device in advance, the distance from the scanning line first detecting the zebra crossing to the imaging device can be obtained, thereby realizing the measurement of the zebra crossing distance. The method recognizes the zebra crossing based on the line-by-line scanning of the image by using the wavelet template, and has the advantages of good anti-jamming ability, high recognition accuracy and high recognition speed duringzebra crossing recognition.

The invention discloses a kit for detecting the content of a serum amyloid protein A. The kit comprises a reagent R1, a reagent R2 and a serum amyloid protein A calibrator, wherein the reagent R1 comprises a first buffer solution, a first electrolyte, a surfactant, a first stabilizer, a high-molecular accelerant and a first preservative; the reagent R2 comprises a second buffer solution, anti-human serum amyloid protein A antibody coated latex particles, a second electrolyte, a second stabilizer and a second preservative; the serum amyloid protein A calibrator comprises a third buffer solution, a third stabilizer, a third preservative, an antioxidant and an anti-human serum amyloid protein A antigen. The invention further discloses an application of the kit for detecting the content of the serum amyloid protein A to the detection of the content of the serum amyloid protein A and a method for detecting the content of the serum amyloid protein A by adopting the kit for detecting the content of the serum amyloid protein A. By adopting the kit and the method disclosed by the invention, the content of the serum amyloid protein A can be detected easily and rapidly.

Disclosed is a nucleic acid amplification method which is based on a new principle and enables to amplify a nucleic acid having a specific nucleotide sequence in a simple manner, within a short time and with efficiency. The nucleic acid amplification method comprises the steps of: (a) conducting a DNA polymerase chain reaction by using, as a template, DNA comprising a nucleotide sequence to be amplified and using a primer pair having a nucleotide sequence complementary to the nucleotide sequence to be amplified, thereby producing a linear DNA fragment; and (b) conducting a chain-substituting DNA polymerase chain reaction in a chaining manner by using cyclic single-stranded DNA comprising the same nucleotide sequence as that of at least one of the primer pair as a template and employing the 3′-terminus of the linear DNA fragment produced in step (a) as the replication origin.

The invention relates to a method and device for detecting the stack overflow position. The method and device are used for rapidly and conveniently detecting the stack overflow position. The method comprises the steps that before a function to be detected is executed, the initial value of a randomly initialized figure in a program is determined, wherein the randomly initialized figure is located at the bite alignment address which is closest to and in front of the last element of an array buffering area of the program; the function to be detected is executed, and after the function to be detected is executed, the current value of the randomly initialized figure is obtained; whether the current value and the initial value are equal or not is judged; if the current value is equal to the initial value, it is determined that stack overflow does not happen to the function to be detected; if the current value is not equal to the initial value, it is determined that stack overflow happens to the function to be detected. By the adoption of the method, the stack overflow position can be rapidly and conveniently detected out.

The invention discloses a method for testing the content of dendrobe polysaccharide content and application thereof. The method comprises the following steps of: extracting dendrobe crude polysaccharide in a hot water extraction method; obtaining the dendrobe essence polysaccharide by virtue of a Sevag method and a dialysis method; and testing conversion factors of the dendrobe and testing contents of the dendrobe polysaccharide from different sources according to conversion factors. The crude polysaccharide and the essence polysaccharide are not needed to renewedly prepare, so that the using cost of a material can be effectively reduced, and the polysaccharide content of the dendrobe can be tested in batch to allow the detection of the dendrobe polysaccharide to be convenient, simple and scientific. The obtained dendrobe essence polysaccharide is high in purity and less in impurities; and compared with the existing methods of ultrasonic extraction, acid-base extraction and the like, the testing method can be used for accurately detecting the content of the dendrobe polysaccharide, providing a reference for identifying the variety of the dendrobe, and also provides effective guidance for breeding, production and market consumption of the dendrobe.

The invention discloses an ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof used for the problem of fast detection for pregnancy, comprising a piece of detecting test paper and urine sample treatment solution; wherein, the detecting test paper consists of a base plate, a cellulose nitrate membrane, a gold label anti-Beta HCG monoclone antibody layer, an Alpha-HCG monoclone antibody line, a sheep anti-rat polyclonal antibody line, an absorbing pad and a sample pad; the matching composition of the urine sample treatment solution is that: 12.5 to 18.0 portions of disodium hydrogen phosphate dodecahydrate, 1.2 to 1.7 portions of sodium dihydrogen phosphate dihydrate, 40.0 to 48.0 portions of sodium chloride and 4600 to 5300 portions of distilled water; the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method of the invention are characterized by fast and simple detection, the operation of blood-drawing detection for 2 to 6 hours can be replaced by 2 to 3 minutes with time-saving, manual-saving and cost-saving; besides, the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof have the advantages of detecting ectopic pregnancy and pregnant days in a semiquantitative way, etc., and can be used for external detection of the pregnant condition and artificial abortion and assisted diagnosis.

The invention aims at providing a fluorescent nano-immunochromatography kit for quickly, sensitively and simply detecting vibrio parahaemolyticus and a preparation method of the kit. In the invention, the separation and enrichment of vibrio parahaemolyticus are realized through specific combination of an immune functionalized time-resolved magnetic fluorescent nanomaterial and the vibrio parahaemolyticus; the captured to-be-detected bacteria are combined with a vibrio parahaemolyticus-specific polyclonal antibody on an immunochromatography test strip; and the detection of the to-be-detected bacteria in the sample is realized by detecting the fluorescence signals on the test strip under an ultraviolet lamp. The kit provided by the invention can be applied to the field of detection on microbial diarrhea-causing pathogenic bacteria.

The invention provides a sewage plant and recycled water plant in-situ sampling and on-line test device and an operation method of the sewage plant and recycled water plant in-situ sampling and on-line test device, and belongs to the technical field of in-situ sampling and test devices. The bottom surface of a conical drill is fixedly connected with a cylindrical sampling rod in a sealing mode, a sampling opening is formed in the lateral side of the bottom end of the sampling rod, a baffle serves as a switch of the sampling opening, and the top of the baffle is connected with a metal rod connected with a handle; a hole plate filter screen is arranged above the sampling opening and is communicated with a silicone tube, and the silicone tube is connected with a water collecting device through a peristaltic pump; the water collecting device is provided with a probe in a sealed mode, and the probe is connected with an instrument. The drill is extended to an appointed depth under a liquid surface, and then the handle is pulled upwards to make the baffle and the sampling opening staggered, a water sample in an in-situ point to be tested flows into the sampling rod under the action of pressure, is filtered through the hole plate filter screen in the rod and then enters the silicone tube, and then the peristaltic pump is opened, and all kinds of indexes of the water sample entering the water collecting device are measured through the probe in real time. The method is flexible, simple, accurate, safe and reliable.

The invention discloses a method and a system for determining sulfide content in water body. The method for determining the sulfide content in the water body comprises the following steps: sampling the sulfide-containing water body, collecting the hydrogen sulfide gas through a chemical reaction; performing spectrum detection on the collected hydrogen sulfide gas, obtaining a hydrogen sulfide gas absorbance spectrum feedback optical signal; transiting the obtained hydrogen sulfide gas spectrum absorbance optical signal to a data signal; analyzing the hydrogen sulfide gas absorbance spectrum feedback data signal, drafting a work curve by taking the concentration of a standard sample as abscissa and taking the absorbance of the standard sample as ordinate, and calculating the sulfide content by determining the absorbance of the sample to be measured. By employing the system for determining the sulfide content in the water body, detection process is simple and fast, the detection result is accurate and reliable, the detection cost is low, analysis operation is simplified, analysis speed is increased, and analysis error is reduced.

The invention relates to a tunnel geological advance forecasting method based on mechanical energy nondestructive detection. The method is characterized in that seismic wave receiving points are distributed on a tunnel face and within range of two-meter-deep non-excavated surrounding rocks around the tunnel face, seismic wave excitation points are distributed on the tunnel axis of certain distance within an excavated range behind the tunnel face and the surrounding range or on a bottom plate parallel to the tunnel axis in turn, and a gasket plate is distributed on the position of each excitation point; and after drilling at the seismic wave receiving points, detectors are distributed in the hole bottoms, and the detectors are connected with a seismic wave acquisition instrument via signal transmission lines so that stable, controllable and safe mechanism energy is utilized to excite seismic wave under the premise of no damage to tunnel lining, seismic reflection wave is enabled to reach receiving holes in "first time" by reasonable distribution of the positions of the excitation points and receiving points and thus tunnel geological advance forecasting can be realized.

The invention discloses a state detection device for a high-potential thyristor. The device comprises a detection circuit board and a detection resistor circuit, wherein the detection circuit board is connected with the detection resistor circuit and connected with a detected thyristor through the detection resistor circuit; the detection resistor circuit comprises detection resistors; the detection circuit board comprises two parallel branches, a first parallel branch comprises a first diode, and a second parallel branch comprises an optical fiber transmitter; when the detected thyristor withstands a forward voltage, current flows through the first diode in the detection circuit board to protect the optical fiber transmitter from withstanding over-high backward voltage; and when the detected thyristor withstands the backward voltage, the current flows through the optical fiber transmitter, the optical fiber transmitter transmits a signal through an optical fiber, and withstanding of the backward voltage by the detected thyristor is detected. The state detection device for the high-potential thyristor is applicable to a high-potential system, a special power circuit is not required to be provided for the detection circuit board, the state of the thyristor can be easily detected, and the optical fiber transmitter can reliably operate in the high-potential system.

A laser beam detection device and method based on an ENZ theory, and belongs to the technical field of laser. The laser beam detection device and method in the prior art has the defects of being cumbersome in operating steps, low in detection precision, hard to improve the resolution ratio, low in signal-to-noise ratio and high in cost. The device charge coupled device (CCD) detector is connected with a data processing computer. An imaging lens is arranged ahead of the CCD detector. The light-sensitive surface of the CCD detector and the focal plane c of the imaging lens are in parallel. The CCD detector is fixed on a slider. A guide slider mechanism is composed of the slider and a guide. The method takes the detected laser beam flare intensity image in the imaging lens focal plane c and the symmetrical front out-of focal plane a and the back out-of focal plane b and adopts an ENZ polynomial structure wave-front fitting coefficient to get an equation set. The multiple wave-front coefficient value correlation of the wave-front of the fitting pupil flat surface laser beam is obtained by solving the equation set, and then the high order error is eliminated by iterative algorithm and the accurate wave-front fitting coefficient value is got, thereby obtaining the phase position and the amplitude of the pupil flat surface laser beam. The characteristic value is calculated through moment method according to phase position and intensity information.

The invention provides a device and method for detecting the performance of functional material activated water, which relate to water treatment. The device mainly comprises six parts including a raw water storage tank, a water treatment bed, an activated water storage tank, a detection and control unit, a water quality detection and control unit and a data processing unit, wherein the potential of hydrogen (pH) values and the oxidation reduction potential (OPR) of raw water and activated water are detected in real time in an on-line way by a raw water pH electrode and a raw water ORP electrode as well as an activated water pH electrode and an activated water ORP electrode; detected data is transmitted into the data processing unit by the water quality detection and control unit; and a real-time data curve and a history data curve can be displayed after data analysis and operation, and test results can be obtained. The device and the method can simply and rapidly detect the performance of the functional material activated water, so that materials with excellent performance for activating animal drinking water can be screened out.

The invention provides a connecting rod cap semi-finished product true position detecting tool. The detection is simple and rapid and therefore the detection efficiency is improved. The connecting rod cap semi-finished product true position detecting tool is suitable for the rapid detection of the connecting rod cap semi-finished product. Meanwhile, the operation is simple and therefore the operating requirement for the testing personnel is reduced and the testing cost is further reduced. The connecting rod cap semi-finished product true position detecting tool comprises a base plate. Footings are installed on the bottom corners of the base plate through bolts. The connecting rod cap semi-finished product true position detecting tool is characterized by comprising a quick clamping, a quick clamping seat, a reference seat, a taper pin and a mechanical watch. One side of the reference seat is fixed on the base plate through bolts, and the other side of the reference seat is located on the base plate in a plug-in mounting mode through two taper pins distributed vertically and uniformly. The two quick clamping seats are longitudinally arranged on two ends of the left side of the reference seat. The quick clamping is fixed on the quick clamping seat through a flange face. The bottom screw holes of the connecting rod cap semi-finished product waiting to be detected are positioned through cone points of taper pins, and the two longitudinal ends of the upper surface is clamped and fixed through the quick clamping.

The invention discloses a pole piece coating thickness online detecting system, which is applied to a coating machine. The pole piece coating thickness online detecting system comprises thickness detecting equipment and a control system; the control system comprises a host computer, a signal processing unit, an operating interface and a control box; two sets of the thickness detecting equipment are respectively arranged in front of and behind the coating machine; the thickness detecting equipment comprises a beta-ray emitting device, a beta-ray receiving device and driving devices; the driving devices are in vertical symmetrical arrangement and respectively drive the beta-ray emitting device and the beta-ray receiving device; the signal processing unit acquires data of the thickness detecting equipment and transmits the data to the host computer for performing analysis processing, judging whether to regulate a pump speed and a blade, and performing pole piece coating thickness cycle control. According to the pole piece coating thickness online detecting system, online measurement of pole piece coating thickness is realized; process parameters can be regulated in time; the stability and the consistency of the coating thickness during a coating process are effectively ensured; the production efficiency and the continuity of a production line are improved.

The invention provides a device and a method for detecting whether any irregular factor such as flaw and adhered dirt existed on each stick-type lens end surfaces forming a lens array simply and quickly with high precision. The device comprises a first light source part (2) for irradiating the detection lights on the normal line of the first stick-type lens end surface forming the stick-type lens array (1) with an angle which is larger than the light-receiving angle of the stick-type lens array; a first light-receiving part (3) for detecting the irradiated lights from the second end surface of the stick-type lens array; and a judging part (6) for judging the stick-type lens array (1) as a poor product when the light intensity detected by the light-receiving part (3) exceeds the preset threshold value.

The invention discloses a method for determining aflatoxin. The method is characterized by comprising the following steps: (1) preparing a solution 1 for later use; (2) preparing a solution 2 for later use; (3) preparing R1 modified by HRP (horseradish peroxidase); (4) preparing R2 modified by HRP; and (5) detecting aflatoxin. Red rhodamine B can neither cause concentration quenching, nor cause insufficient light intensity. By adding mica powder, the luminescence lifetime of an oxalate luminescence system is significantly prolonged. Ruthenium terpyridine and chemiluminescence have the advantages of high detection sensitivity, wide linear range and the like, and aflatoxin can be detected more effectively. The method disclosed by the invention has the excellent properties of lower toxicity, higher flash point and lower vapor pressure. By detecting the content of aflatoxin in liquid milk and using a standard addition recovery method, the recovery yield of 98.4%-100.4% is obtained, indicating that the method has good accuracy in determination of aflatoxin.