Major QTL (quantitative trait loci) of thousand seed weight trait of Brassica napus L., and SNP molecular markers and application thereof

Abstract

The invention provides a major QTL (quantitative trait loci) of thousand seed weight trait of Brassica napus L., which is between the 27906310th base and the 28240646th base of the chromosome A09 of Brassica napus L.. Preferably, the rate of contribution of the major QTL to the thousand seed weight trait of Brassica napus L. is 37.4%; the the major QTL to the thousand seed weight trait of Brassicanapus L. is in close linkage with a first SNP molecular marker, the first SNP molecular marker is located at the 27906310th base which refers to T or G, and the mutation causes polymorphism; the major QTL to the thousand seed weight trait of Brassica napus L. is in close linkage with a second SNP molecular marker, the second NP molecular marker is located at the 28240646th base which refers to Tor G, and the mutation causes polymorphism; the major QTL to the thousand seed weight trait of Brassica napus L. is in close linkage with a peak SNP molecular marker, the peak SNP molecular marker islocated at the 28151624th base which refers to T or G, and the mutation causes polymorphism. The invention further provides related SNP molecular markers and application thereof. The major QTL of thousand seed weight trait of Brassica napus L. has high rate of contribution to the thousand seed weight trait of Brassica napus L., plays a key role in regulating and controlling the thousand seed weight trait of Brassica napus L., can be used for map-based cloning and molecular marker assistant selection, and is suitable to be promoted and applied in scale.

Description

technical field [0001] The invention relates to the technical field of molecular biology and rapeseed breeding, in particular to the technical field of thousand-grain weight traits of Brassica napus, specifically refers to a main effect QTL site, SNP molecular marker and application of the thousand-grain weight trait of Brassica napus. Background technique [0002] Rapeseed belongs to Brassica (Cruciferae) and is an important excellent oil crop. According to botanical and biological characteristics, rapeseed can be divided into three main types: Brassica campestdris, L.; 2n=20, aa, Brassica juncea, Coss.; 2n=36, aabb. and Brassica napus (Brassica napus, L.; 2n=38, aacc). Brassica napus is now one of the most important oil crops in the world, especially in Asia, Europe and North America. It is also the largest oil crop in my country. Rapeseed oil accounts for more than 55% of the domestic edible vegetable oil. In recent years, with rising labor prices and production costs, ...

AI Technical Summary

Problems solved by technology

The research on thousand-grain weight of rapeseed is still in its infancy. Therefore, one of the major problems to be solved in high-yield breeding of rapeseed in my country is to discover more thousand-grain weight genes and improve rapeseed varieties.

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