Method for testing content of dendrobe polysaccharide and application thereof

A technology of dendrobium polysaccharide and determination method, applied in the field of determination of dendrobium polysaccharide content, can solve problems such as time-consuming, consumables, labor consumption, etc., and achieve the effects of reduced use cost, convenient detection and high purity

Active Publication Date: 2013-02-13
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acquisition of dendrobium polysaccharides is a very complicated process, which requires steps such as hot water extraction, crude polysaccharide extraction, and protein removal. It takes about 500 g of fresh dendrobium polysaccharides to prepare 1 g of refined polysaccharides. The traditional preparation and determination methods are time-consuming and expensive. Disadvantages of power consumption and consumables

Method used

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  • Method for testing content of dendrobe polysaccharide and application thereof
  • Method for testing content of dendrobe polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of assay method of dendrobium officinale polysaccharide content comprises the following steps:

[0036] (1) Preparation of Dendrobium officinale powder: select mature plants of Dendrobium officinale Kimura et Migo from Yunnan, kill green at 105°C for 15 minutes, dry at 60°C to constant weight, grind into powder, pass through a 60-mesh sieve to obtain iron sheet Dendrobium dry powder;

[0037] (2) Preparation of crude polysaccharide: Weigh 50g of dry powder of Dendrobium candidum, add 750ml of petroleum ether (boiling point 60-90°C), reflux extraction at 80°C for 2 times, each time for 1h, obtain filter residue A after suction filtration; add to filter residue A 750ml 80wt% ethanol solution, reflux extraction at 80°C for 2 times, each time for 1h, obtain filter residue B after suction filtration, dry filter residue B at 60°C to obtain degreasing dry powder; add 750ml distilled water to the degreasing dry powder, reflux extraction twice at 80°C , 1 hour each time,...

Embodiment 2

[0046] A method for measuring the polysaccharide content of hybrid dendrobium, comprising the following steps:

[0047] (1) Preparation of Dendrobium powder: select 2-year-old mature stem segments of wild Yunnan Dendrobium nobile Lindl (Dendrobium nobile Lindl) that have been domesticated for two years, kill at 105°C for 15 minutes, dry at 60°C to constant weight, grind into powder, and pass through 60 mesh Sieve to obtain the dry powder of wild Dendrobium nobile;

[0048] The mature stems of 36 kinds of hybrid Dendrobium (Nobile-type Dendrobium) introduced from Japan and the mature stems of tissue-cultured Nobile (both 2 years old) were taken respectively, killed at 105°C for 15 minutes, dried at 60°C to constant weight, and ground into Powder, cross 60 mesh sieves to get the dry powder of hybrid dendrobium;

[0049] (2) Preparation of crude polysaccharide: same as step (2) of Example 1, the only difference is that the powder used is dry powder of wild Dendrobium nobile;

...

Embodiment 3

[0064] A method for assaying polysaccharide content of Dendrobium drumsticks, comprising the following steps:

[0065] (1) Preparation of Dendrobium powder: select 2-year-old mature stem segments of Yunnan wild Dendrobium chysotoxum Lindle that have been domesticated for two years, kill at 105°C for 15 minutes, dry at 60°C to constant weight, grind into powder, and pass through 60 mesh Sieve to obtain the dry powder of wild Dendrobium chrysanthemum;

[0066] (2) Preparation of crude polysaccharide: same as step (2) of Example 1; the only difference is that the powder used is the dry powder of wild Dendrobium drumstick;

[0067] (3) Preparation of refined polysaccharide: the operation is the same as step (3) of Example 1;

[0068] (4) Preparation of sample solution: the operation is the same as step (4) of Example 1;

[0069] (5) Determination of Dendrobium polysaccharide content by improved phenol-sulfuric acid method:

[0070] Preparation of standard curve: absorb 0.05mg·m...

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Abstract

The invention discloses a method for testing the content of dendrobe polysaccharide content and application thereof. The method comprises the following steps of: extracting dendrobe crude polysaccharide in a hot water extraction method; obtaining the dendrobe essence polysaccharide by virtue of a Sevag method and a dialysis method; and testing conversion factors of the dendrobe and testing contents of the dendrobe polysaccharide from different sources according to conversion factors. The crude polysaccharide and the essence polysaccharide are not needed to renewedly prepare, so that the using cost of a material can be effectively reduced, and the polysaccharide content of the dendrobe can be tested in batch to allow the detection of the dendrobe polysaccharide to be convenient, simple and scientific. The obtained dendrobe essence polysaccharide is high in purity and less in impurities; and compared with the existing methods of ultrasonic extraction, acid-base extraction and the like, the testing method can be used for accurately detecting the content of the dendrobe polysaccharide, providing a reference for identifying the variety of the dendrobe, and also provides effective guidance for breeding, production and market consumption of the dendrobe.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis of traditional Chinese medicines and extracts thereof, and in particular relates to a method for determining polysaccharide content of Dendrobium dendrobii and its application. Background technique [0002] Polysaccharides are formed by the condensation and dehydration of multiple monosaccharide molecules. They are a type of carbohydrates with complex molecular structures and large molecular weights, with relative molecular masses ranging from tens of thousands to tens of millions. Polysaccharides are widely distributed in nature and are also very important. Some are components that constitute the skeleton structure of animals and plants, such as cellulose; some are stored as nutrients for animals and plants, such as glycogen and starch; Heparin has anticoagulant effect, and polysaccharides in pneumococcal cell wall have antigenic effect. In recent years, polysaccharide research has bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 王再花朱根发叶庆生章金辉操君喜孙延东
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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