266 results about "Dialysis method" patented technology

Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stable.

The invention relates to a method for removing heavy metal ions in water by using a graphene oxide sheet, which belongs to the technical fields of water purification and environmental protection. In the invention, the graphene oxide sheet is used as an adsorbent of the heavy metal ions, wherein the graphene oxide sheet is of a monatomic layer two-dimensional structure and has a huge specific surface while ensuring no obvious toxicity. In the purification process of the method, the operations are simple without pollution, and the copper ion adsorption efficiency is 10 times that of the active carbon. In the invention, the graphene oxide sheet adsorbing the metal ions can be recovered to the single sheet structure by a centrifugal separation or dialysis method and can be recycled.

A method and apparatus for replacement of renal function by periodically removing a substantial volume of patient's blood and simultaneously replacing it with the reconstituted blood. Reconstituted blood consists of the patient's own condensed blood cells and proteins diluted with the sterile physiologic solution. As a result, small molecules and excess water are periodically removed and discarded. Blood cells and proteins are safely stored to be used at the next therapy session.

Cartridges useful in regenerating or purifying dialysis solutions are described as well as methods to regenerate or purify spent dialysis solutions. Dialysis methods using the sorbent cartridges of the present invention are further described.

The invention discloses a star polymer nano-medicament carrier preparation used for intracellular medicament delivery. The nano-medicament carrier preparation is characterized by comprising the following components in part by weight: 1 to 20 parts of medicament, 10 to 200 parts of amphiphilic star segmented copolymer, and 20 to 500 parts of organic solvent, wherein the amphiphilic star segmented copolymer in the carrier is formed from hydroxyl-terminated star aliphatic polyester by connecting a hydrophilic segment through a disulfide bond. By using a self-assembling behavior of the copolymer in aqueous solution, nanoparticles of which internal hydrophobic layers coat the medicament can be prepared by a dialysis method. After entering cells, the nano-medicament carrier can prompt a hydrophilic shell thereof to drop selectively by using a reducing environment in tumor cells so as to realize localized release of the medicament in the tumor cells.

The invention discloses preparation and application of an insoluble drug-entrapped poloxamer/amphiphilic polysaccharide mixed micelle. The insoluble drug-entrapped poloxamer/amphiphilic polysaccharide mixed micelle is prepared through a dialysis method or a solvent evaporation method. The mixed micelle is low in critical micelle concentration, is high in drug-loading rate, is capable of obviously prolonging the stabilization time and has the long-circulation function of a nanomicelle and has dual functions of restraining the metabolism of P-glycoprotein and cytochrome P450 enzyme and is capable of increasing the bioavailability of oral administration. The mixed micelle is simple in preparation method, is mature in process and is high in yield and can be prepared into preparations for the oral administration, such as tablets, capsules, pills and syrups.

The invention relates to pH-triggering-to-release multilevel targeting polymer micelle in tumor cells and a preparation method of the multilevel targeting polymer micelle. The micelle is formed by self assembly of hydrophobic modified polysaccharide polymer with an endosome pH sensitive characteristic in a water medium. A hyaluronic acid-deoxycholate-histidine polymer is used as a carrier, and caner initiatively-targeting endosome pH sensitive polymer micelle is prepared by virtue of an ultrasonic method or a dialysis method to wrap an antitumor drug difficult to dissolve. By using the synergistic mechanism of EPR-mediated passive targeting, CD44 receptor initiative targeting and pH sensitive targeting strategies, the 'whole-course targeting' guidance is performed at four medicine delivery key steps, i.e., blood long circulation, tumor tissue accumulation, cell digestion and intracellular drug release, so that multilevel targeting drug delivery is realized, the intracellular drug concentration is effectively increased, and a novel carrier and a preparation strategy are provided for improving the antitumor effect of the difficultly-soluble antitumor drug.

An automatic dialyzer for purifying blood brought into contact with a dialysate via a semi-impermeable membrane. The dialyzer includes at least a blood circulating system and a dialysate supply/discharge system, wherein the blood line of the blood circulating system is connected to an overflow line having a reversibly-rotatable blood pump, a blood chamber, and an open/close device for overflowing of liquid from the blood chamber to the outside of the blood line. Liquid supply means is provided to a first bypass line and/or a second bypass line for supplying liquid in both forward and reverse directions to enable regulation of the supply amount of the dialysate for water removing/liquid replenishing. The supply speed of the liquid supply device and the supply speed of the blood pump are regulatable in conjunction with each other. Also provided is a dialyzing method using the automatic dialyzer.

The invention relates to a dry preparation method of transition metal doped carbon fluorescent quantum dots. According to the method, a metal chelator and transition metal salt are subjected to a thermal reaction in the absence of a solvent, and the transition metal doped carbon fluorescent quantum dots are prepared with extraction, centrifugation and dialysis methods after the reaction. The method is convenient to operate, doping of carbon fluorescent quantum dots with metal ions can be realized without harsh reaction conditions or large instruments, and the obtained carbon dots have good water solubility and wider fluorescence emission ranges. The obtained carbon dots have multiple different characteristics according to different types of doping metal ions and different solvents. The prepared carbon dots have great application value in preparation of bio-labeling sensing and medical imaging, photoelectric and light-emitting devices and the like due to these characteristics.

The invention discloses a carboxymethyl cellulose grafted polylactic acid amphiphilic polymer, as well as a preparation method and an application thereof. The method comprises the following steps: dissolving carboxymethyl cellulose in an ionic liquid to form a homogeneous solution; adding grafting monomers, namely L-lactide monomers and a catalyst, namely stannous octoate into the homogeneous solution; controlling the temperature at 100 DEG C-130 DEG C, performing magnetic stirring, reacting for 18-24 hours in the presence of nitrogen, stopping the reaction and reducing the temperature of a system to room temperature; pouring the reaction system into ethanol to produce a precipitate, filtering and separating the precipitate, and washing with anhydrous ethanol; performing extraction on an obtained product in acetone, and performing vacuum drying to get the purified carboxymethyl cellulose grafted polylactic acid amphiphilic polymer. A dialysis method is utilized for forming self-assembled nanosphere-like micelles of the carboxymethyl cellulose grafted polylactic acid amphiphilic polymer, the particle size is 30-150nm, the critical micelle concentration is 0.01-0.1g/L and the anti-dilution stability is good.

The invention discloses a focused ultrasound-polymeric micelle controllable drug release device and a release method thereof. The device is characterized by consisting of a focused ultrasound system and a drug loaded polymeric micelle system, wherein the focused ultrasound system is connected with a focused ultrasound energy converter (3) by a signal generator (1) through a power amplifier (2); the drug loaded polymeric micelle system consists of a complex micelle (5) formed by amphiphilic block copolymers and drugs and a medium (4); and a drug loaded polymeric micelle is internally provided with a sensor (6) which is connected with a computer (8) through a signal processor (7). The focused ultrasound-polymeric micelle controllable drug release method comprises the step that the drug carried polymeric micelle is prepared through a dialysis method or a solvent evaporation method, and under ultrasonic irradiation, the polymeric micelle responds to ultrasound through a physical or chemical change so as to ensure that loaded hydrophobic drugs are released from the micelle and enter a focal part.

The invention relates to a drug delivery system of nanometer liver targeting amphiphilic block copolymer and a preparation method thereof. The nanometer liver targeting drug delivery system is obtained by adopting liver targeting amphiphilic block copolymer which can be biologically degraded and which has good biocompatibility as the carrier material and embedding drugs for treating liver diseases by a solvent dialysis method or a solvent evaporation method; the weight ratio of the carrier material to the drug is 1:0.1 to 1.2. The drug delivery system has the advantages of simple operation, mild condition and cheap raw material; the prepared drug delivery system has good biocompatibility and both effects of active targeting and passive targeting, and can realize high targeting and sustained release drug delivery to the liver by means of injection, thus solving the defects of bad targeting and low bioavailability of present anticancer drugs and improving the life quality of patients, with broad application prospects.

The invention relates to a detecting method for selenium speciation analysis in selenium-rich rice and particularly relates to a detecting method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-rich rice. The measuring method comprises the following steps of: (1) preparing a sample, namely sealing and storing selenium-rich rice flour to be detected for future use, wherein organic selenium is extracted through a dialysis method, and protein selenium, polysaccharide selenium and RNA selenium are respectively extracted; (2) measuring the selenium content through hydride atomic fluorescence spectrometry under measuring conditions that the negative high pressure is 340V, the lamp current is 100mA, the atomization temperature is 800 DEG C, the furnace height is 8mm, the flow rate of carrier gas is 500mL/min, the flow rate of shielding gas is 1000mL/min, the delay time is 1 second, the reading time is 10 seconds, the liquid adding time is 8 seconds, and the sample feeding volume is 2mL; and (3) data processing. The detecting method disclosed by the invention has the advantages of high sensitivity and accuracy and simplicity in operation.

The invention relates to medicine preparation which is characterized by having a special physical shape, and further relates to a pro-medicine of curcumin micelle with high medicine loading and a preparation method of the pro-medicine. The preparation method comprises the following steps of: bonding the curcumin modified by flutaric anhydride and MPEG-PLA (Methoxy poly(ethylene glycol)-poly(lactic acid)) with polyhydroxy distal end in an ester bond manner to form a curcumin micelle monomer with high grafting percent; and preparing medicine-loading micelle of the curcumin through a dialysis method. The pro-medicine of the curcumin micelle with the high medicine loading, obtained by the method disclosed by the invention breaks through a medicine administration mode of a traditional carrier-coating medicine so that a medicine is a part of a carrier; in addition, by controlling the usage of inert carrier materials, the medicine loading, the grain diameter and the stability of a micelle system are remarkably modified, and an EPR (Electron Paramagnetic Resonance) effect is reinforced.

The invention relates to a detection method based on selenium form analysis in marine products, in particular to a method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS) way. The method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-ICP-MS way comprises steps as follows: 1, preparing a sample: removing shell of the sample, storing into a sample bag; (1) separating and purifying organic selenium: carrying out a continuous dialysis method to separate and purify the organic selenium; (2) separating and purifying protein selenium; and (3) separating and purifying polysaccharide selenium; and 2, detecting the content of selenium: (1) performing microwave digestion; (2) implementing blank test: processing an agent without being contacted with a sample to be detected according to the method in sub-step (1) of step 2; and (3) detecting by inductively coupled plasma mass spectrometry. The method has the advantages that the organic selenium, the protein selenium or the polysaccharide selenium in the marine products can be efficiently, simply, conveniently and quickly detected.

The invention discloses a solar energy and osmotic energy (reverse electro dialysis method) combined power generation device which comprises a solar energy distillation device and an osmotic energy reverse electro dialysis device. The bottom of a pool body of the osmotic energy reverse electro dialysis device is black, light absorption ability of the black is good, the block and an obliquely-arranged light-transmitting plate located at the top of the pool body jointly play the effect to absorb sunlight, and water vapor evaporated from a seawater pool and a fresh water pool is condensed on the light-transmitting plate to flow into the fresh water pool. The solar energy and osmotic energy reverse electro dialysis method combined power generation device has the advantages that due to the fact that fresh water evaporated from the seawater pool flows into the fresh water pool, the power generating process implementation can be retarded in the process, the fresh water salinity of the fresh water pool is improved, the seawater of the seawater pool is reduced, and accordingly osmotic energy generating efficiency is improved.

The present invention is the preparation process of hollow microspheric polyorganosilane molecular box, and features that on O/W type microemulsion polydialkyl siloxane template kernel, organosilicon monomer with D-type functional group and T-type functional group is added for condensation around the surface of the template kernel to form polyorganosilane microsphere in core-shell structure, and then solvent swelling dialysis method is adopted to eliminate the template kernel and to obtain hollow microspheric molecular box. The hollow microspheric polyorganosilane molecular box thus prepared has average diameter of 80-180 nm, good swelling and coating performance, and may be surface modified to reach required hydrophilic and lipophilic property. The hollow microspheric polyorganosilane molecular box has good biocompatibility, biodegradability and physiological inertia, and may be used widely.

The invention discloses a magnetic carboxylation hollow microsphere soil restoration agent and a preparation method thereof. The method comprises the following steps of using improved Stober method for preparing single dispersion SiO2 microspheres; using a hydrothermal method for synthesizing Fe3O4@C hollow microspheres; using a dialysis method for synthesizing Fe3O4@C-COOH hollow microspheres. The invention also provides application of the magnetic carboxylation hollow microsphere soil restoration agent to lead pollution soil restoration. The improved Stober method, the hydrothermal method and the dialysis method are integrated; the synthesized carboxylation hollow microspheres have the unique core-sell structures; a great number of -COOH and -OH groups are coated on the surface; the advantages of great adsorption volume, high specificity and the like are realized; the adsorption volume on heavy metal lead in the soil is much higher than the adsorption volume of synthesis materials prepared a coprecipitation method, a micro emulsion method and a thermolysis method. The carboxylation hollow microsphere soil restoration agent prepared by the method also has a certain recovery performance and can be reused.

The invention discloses a method for improving the hydrophilicity of a polypeptide membrane by using polylactic acid and polyacrylic acid. The method comprises the following steps: (1) adding a polypeptide homopolymer, diisocyanate, a catalyst and a solvent into a dry reactor, reacting for 40-50 minutes at the temperature of 40-50 DEG C, and then, removing superfluous diisocyanate by a dialysis method, so as to obtain a polypeptide homopolymer containing terminal -NCO groups; (2) adding the polypeptide homopolymer containing terminal -NCO groups, a catalyst and a solvent into a dry reactor, then, adding polylactic-acid mono tetradecyl ether, and reacting for 50-60 minutes at the temperature of 40-50 DEG C, so as to obtain a polypeptide-polylactic acid diblock copolymer; (3) adding the polypeptide-polylactic acid diblock copolymer, polyacrylic acid and a solvent into a dry reactor, stirring and mixing for 60-70 minutes at the temperature of 40-50 DEG C, then, carrying out membrane forming by a tape casting method, and drying, thereby obtaining a target product of the method. According to the method, the preparation process is simple, and the hydrophilicity of the obtained modified membrane is improved greatly.

The invention discloses a pH response/ hydrophobic group random copolymerization polymer, and a preparation method and application thereof. A hydrophobic group and pH response group random copolymer is obtained through electron transfer activator regeneration-atom transfer radical polymerization and ring-opening polymerization, and a pH response amphiphilic polymer brush is prepared by triggeringthe activators regenerated by electron transfer (ARGET) atom transfer radical polymerization (ATRP) of hydrophilic macromolecule monomers by the hydrophobic group and pH response group random copolymer used as macroinitiator, and performing selective hydrolysis reaction. The proportion of functional groups in the polymer molecule is easy to adjust and control; and a synthesis process is simple and the yield is high. Polymer micelle with an inner core formed by hydrophobic groups and a pH response groups, and an outer shell formed by hydrophilic groups can be obtained by a dialysis method; anda water insoluble medicament can be entrapped in the inner core of the micelle, the medicament can be subjected to sustained release in gastric juice, and can be released quickly and controllably under intestinal canal conditions.

The invention discloses a preparing method for dialdehyde nano-crystalline cellulose. The preparing method comprises the steps that firstly, nano-crystalline cellulose is prepared; secondly, the dialdehyde nano-crystalline cellulose is prepared; thirdly, the dialdehyde nano-crystalline cellulose suspension liquid prepared in the second step is washed through a dialysis method, and the dialdehyde nano-crystalline cellulose is obtained. The preparing method has the following advantages that preparation is simple, the obtained dialdehyde nano-crystalline cellulose is high in aldehyde group content and is a multifunctional product which has wide application prospects in the biomedical field and chemical engineering field.