Process for simultaneously extracting pigments, saponins and polysaccharides of sapindus, and preparation and application of saponins of sapindus

Abstract

The invention relates to a process for simultaneously extracting pigments, saponins and polysaccharides of sapindus, and preparation and application of the saponins of the sapindus, and belongs to the technical field of medicine and chemical engineering. The process includes extracting total extracts of the sapindus from dry fruit peel of the sapindus by the aid of a decocting method, dissolving the total extracts in water to obtain samples, feeding the samples to macroporous adsorption resin, eluting the macroporous adsorption resin by distilled water at first, collecting eluent, removing proteins by the aid of a dialysis method, and concentrating and drying the eluent to obtain polysaccharide extracts of the sapindus; eluting the macroporous adsorption resin with 4-5 retention volumes by the aid of 40% ethanol, then eluting the macroporous adsorption resin by the aid of 80% ethanol, collecting eluent and concentrating and drying the eluent to obtain saponins extracts of the sapindus; eluting the macroporous adsorption resin by the aid of 95% ethanol, collecting eluent and concentrating and drying the eluent to obtain pigment extracts of the sapindus. Active dry yeast is inoculated in the saponins extracts of the sapindus after the saponins extracts of the sapindus are dissolved in water, then the saponins extracts of the sapindus are fermented and are concentrated and dried to obtain the saponins of the sapindus. The process, the preparation and the application have the advantages of simplicity in preparation method, low cost, one-step reaction, environmental protection, high efficiency and easiness in popularization and application.

Description

technical field [0001] The invention belongs to the technical field of medicine and chemical industry, and specifically relates to a process for simultaneously extracting sapindus pigment, saponin and polysaccharide, a method for extracting sapindus saponin by fermentation, and its application in food and daily necessities. Background technique [0002] Sapindus delavayi is a plant of the genus Sapindus in the family Sapindaceae, also known as Sapindus delavayi and Sapindus yunnanensis. Mainly distributed in Yunnan, Sichuan and other provinces. The seeds are used for medicine and can repel insects; the seed oil can be used to make hardened oil; the peel can be used as a substitute for soap, which can produce rich foam and strong detergency. It is an excellent natural surfactant, especially suitable for daily necessities such as washing and bathing toothpaste of high-grade silk, pure wool fabrics and baby products. In addition, sapindus saponin itself has strong antibacteria...

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction methods of natural saponins are still limited to traditional water extraction and alcohol precipitation methods, organic solvent extraction methods, etc., which cause large losses, high energy consumption, and increase costs, which is not conducive to the competition between natural saponins and chemical surfactants.

Saponins are easily soluble in water. Use water to extract total saponins, and then use ethanol to remove impurities such as starch, protein, and polysaccharides, but ethanol will also destroy the polymer compounds that form the saponin colloidal stable system, and the product will appear precipitation, agglomeration, etc.; Using solvent extraction, the yield is low. For example, using n-butanol to extract Sapindus sapindus saponins, the yield is only about 27%, and there are still solvent residues, which is not conducive to environmental protection.

Images