Content measuring method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-rich rice

A technology for selenium-enriched rice and its determination method, which is applied to the detection of protein selenium, polysaccharide selenium or RNA selenium, and the field of organic selenium in selenium-enriched rice, which can solve the problems that beneficial selenium forms are rarely involved, and achieve convenient implementation and high accuracy , the effect of low detection cost

Inactive Publication Date: 2013-07-24
QUZHOU QUALITY & TECH SUPERVISION & INSPECTION CENT
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Problems solved by technology

[0003] At present, domestic and foreign studies on selenium-enriched agricultural products and foods are rare, and the detection and analysis of benefici

Method used

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  • Content measuring method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-rich rice
  • Content measuring method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-rich rice
  • Content measuring method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-rich rice

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Embodiment

[0033] A method for assaying organic selenium, protein selenium, polysaccharide selenium or RNA selenium content in selenium-enriched rice, said assay method comprising the following steps:

[0034] (1) Preparation of samples: crush the selenium-enriched rice to be tested to obtain dry selenium-enriched rice powder with a particle size of 100 mesh, and seal and store it for later use;

[0035] ① Extraction of organic selenium: Weigh 1.00g of the above-mentioned selenium-enriched rice dry powder, add 10ml of ultrapure water, further grind to fully break the selenium-enriched rice dry powder, and quantitatively transfer it to a dialysis bag. The specification of the dialysis bag is 8000Da. Dialyze for 24 hours, replace the ultrapure water by centrifugation every 6 hours, take out the sample after dialysis, and centrifuge at 12,000rpm for 15 minutes to obtain a precipitate, which is dried under the condition of vacuum degree of -0.085Mpa and temperature of 60°C to obtain an organi...

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Abstract

The invention relates to a detecting method for selenium speciation analysis in selenium-rich rice and particularly relates to a detecting method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-rich rice. The measuring method comprises the following steps of: (1) preparing a sample, namely sealing and storing selenium-rich rice flour to be detected for future use, wherein organic selenium is extracted through a dialysis method, and protein selenium, polysaccharide selenium and RNA selenium are respectively extracted; (2) measuring the selenium content through hydride atomic fluorescence spectrometry under measuring conditions that the negative high pressure is 340V, the lamp current is 100mA, the atomization temperature is 800 DEG C, the furnace height is 8mm, the flow rate of carrier gas is 500mL/min, the flow rate of shielding gas is 1000mL/min, the delay time is 1 second, the reading time is 10 seconds, the liquid adding time is 8 seconds, and the sample feeding volume is 2mL; and (3) data processing. The detecting method disclosed by the invention has the advantages of high sensitivity and accuracy and simplicity in operation.

Description

technical field [0001] The invention relates to a detection method for selenium form analysis in selenium-enriched rice, in particular to a detection method for organic selenium, protein selenium, polysaccharide selenium or RNA selenium in selenium-enriched rice. Background technique [0002] Selenium is one of the essential trace elements in the life activities of the human body, and has received widespread attention in recent years. It has a variety of physiological functions such as anti-aging, preventing cancer, protecting and repairing vegetative cells, detoxifying and detoxifying, and improving immunity. It is known as one of the elements that dominate life. The importance of selenium to the human body is very clear, but authoritative data show that the function of selenium varies with its form. The existence of inorganic selenium in food is harmful to the human body. The inorganic form of selenium, especially hexavalent selenium, is very toxic and has The human body ...

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
Inventor 祝华明方建军张旭郑睿行鲍利锋龚鸿萍方芳夏爱萍
Owner QUZHOU QUALITY & TECH SUPERVISION & INSPECTION CENT
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