Method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-ICP-MS (Inductively Coupled Plasma Mass Spectrometry) way

A technology of ICP-MS and microwave digestion, applied in the detection field of selenium speciation analysis in seafood

Inactive Publication Date: 2013-02-13
中华人民共和国浙江出入境检验检疫局
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Problems solved by technology

There are no literature reports on the direct determination of organic selenium, protein selenium and polysaccharide selenium in seafood by microwave digestion-ICP-MS at home and abroad

Method used

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  • Method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-ICP-MS (Inductively Coupled Plasma Mass Spectrometry) way

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Embodiment Construction

[0048] A detailed description will be given below of specific embodiments of the present invention.

[0049] The microwave digestion-inductively coupled plasma mass spectrometry method of the present invention directly measures the detection method of organic selenium, protein selenium and polysaccharide selenium in seafood, and the method comprises the following steps:

[0050] 1. Sample preparation

[0051] The samples were shelled, and all the edible parts were fully crushed and mixed with a beater, put into sample bags, and stored at -18°C for later use (as

[0052] for the sample).

[0053] (1) Separation and purification of organic selenium

[0054] Using the continuous dialysis method, accurately weigh 20.0g (accurate to four decimal places) of the sample and put it into a dialysis bag.

[0055] Dialyze for 96 hours (to remove small molecules), and change the water every 12 hours. The samples after dialysis were vacuum freeze-dried and stored at -18°C for future us...

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Abstract

The invention relates to a detection method based on selenium form analysis in marine products, in particular to a method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS) way. The method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-ICP-MS way comprises steps as follows: 1, preparing a sample: removing shell of the sample, storing into a sample bag; (1) separating and purifying organic selenium: carrying out a continuous dialysis method to separate and purify the organic selenium; (2) separating and purifying protein selenium; and (3) separating and purifying polysaccharide selenium; and 2, detecting the content of selenium: (1) performing microwave digestion; (2) implementing blank test: processing an agent without being contacted with a sample to be detected according to the method in sub-step (1) of step 2; and (3) detecting by inductively coupled plasma mass spectrometry. The method has the advantages that the organic selenium, the protein selenium or the polysaccharide selenium in the marine products can be efficiently, simply, conveniently and quickly detected.

Description

technical field [0001] The invention relates to a detection method for the speciation analysis of selenium in seafood, in particular to a detection method for directly determining organic selenium, protein selenium or polysaccharide selenium in seafood by using a microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS) method. Background technique [0002] The trace element selenium is an essential nutrient element for organisms, but excessive selenium can cause poisoning. Therefore, selenium is an element with both beneficial and toxic properties. Excessive or insufficient intake will cause damage to the body and cause various disease. Due to the narrow concentration boundary between beneficial and toxic selenium, the recommended daily intake of selenium is limited to 50-200 μg / d. In addition, the dual nature of selenium is related to the form in which it exists and its content. [0003] The existing forms of selenium are mainly divided into inorganic sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/34
Inventor 鲁丹张虹徐庆兵俞琰垒郭利攀
Owner 中华人民共和国浙江出入境检验检疫局
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