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Kit and method for detecting content of serum amyloid protein A and application

A technology for amyloid protein and serum starch, which is applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of inaccurate quantification, high reagent cost, slow test speed, etc., and achieves high detection efficiency, high sensitivity, and high detection efficiency. Efficiency improvement effect

Inactive Publication Date: 2016-05-04
潍坊三维生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the above methods, the enzyme-linked immunoassay has an enzymatic amplification effect, but the quantification is not accurate enough and the sensitivity is low; the immunoscattering turbidimetry requires a special special protein instrument, the reagent cost is high, and the test speed is slow

Method used

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  • Kit and method for detecting content of serum amyloid protein A and application
  • Kit and method for detecting content of serum amyloid protein A and application
  • Kit and method for detecting content of serum amyloid protein A and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The kit for detecting serum amyloid A content includes: reagent R1, reagent R2 and serum amyloid A calibrator.

[0066] Wherein, reagent R1 comprises the following components:

[0067]

[0068] Reagent R2 includes the following components:

[0069]

[0070] Serum Amyloid A calibrator includes the following components:

[0071]

[0072]

[0073] Add the corresponding amount of serum amyloid A antigen to the above-mentioned other reagent mixture according to the required concentration of the calibrator, and mix to obtain the serum amyloid A calibrator. In this example, 5 serum amyloid A calibrators with different concentrations were prepared, and the concentrations of the serum amyloid A antigens were: 10 mg / L, 45 mg / L, 90 mg / L, 120 mg / L and 180 mg / L. The serum amyloid A calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C.

[0074] Using the above kit, the samples to be tested were detected and analyzed by a Hitachi 7...

Embodiment 2

[0076] The kit for detecting serum amyloid A content includes: reagent R1, reagent R2 and serum amyloid A calibrator.

[0077] Wherein, reagent R1 comprises the following components:

[0078]

[0079] Reagent R2 includes the following components:

[0080]

[0081] Serum Amyloid A calibrator includes the following components:

[0082]

[0083] In this embodiment, a high-concentration single-point calibrator is selected, so the serum amyloid A antigen concentration is 180 mg / L. Then the serum amyloid A calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C. Serum amyloid A calibrator diluted with normal saline to 5 different concentrations when used, the concentrations of serum amyloid A antigen are: 10mg / L, 45mg / L, 90mg / L, 120mg / L and 180mg / L.

[0084] Using the above kit, detection and analysis were carried out by Hitachi 7080 automatic biochemical analyzer. The detection method is the same as in Example 1.

Embodiment 3

[0086] The kit for detecting serum amyloid A content includes: reagent R1, reagent R2 and serum amyloid A calibrator.

[0087] Wherein, reagent R1 comprises the following components:

[0088]

[0089]

[0090] Reagent R2 includes the following components:

[0091]

[0092] Serum Amyloid A calibrator includes the following components:

[0093]

[0094]

[0095] Add the corresponding amount of serum amyloid A antigen to the above-mentioned other reagent mixture according to the required concentration of the calibrator, and mix to obtain the serum amyloid A calibrator. In this example, 5 serum amyloid A calibrators with different concentrations were prepared, and the concentrations of the serum amyloid A antigens were: 10 mg / L, 45 mg / L, 90 mg / L, 120 mg / L and 180 mg / L. The immunoglobulin E was sterilized by filtration through a 0.22 μm filter membrane, and stored at 2-8°C.

[0096] Using the above kit, detection and analysis were carried out by Hitachi 7080 autom...

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Abstract

The invention discloses a kit for detecting the content of a serum amyloid protein A. The kit comprises a reagent R1, a reagent R2 and a serum amyloid protein A calibrator, wherein the reagent R1 comprises a first buffer solution, a first electrolyte, a surfactant, a first stabilizer, a high-molecular accelerant and a first preservative; the reagent R2 comprises a second buffer solution, anti-human serum amyloid protein A antibody coated latex particles, a second electrolyte, a second stabilizer and a second preservative; the serum amyloid protein A calibrator comprises a third buffer solution, a third stabilizer, a third preservative, an antioxidant and an anti-human serum amyloid protein A antigen. The invention further discloses an application of the kit for detecting the content of the serum amyloid protein A to the detection of the content of the serum amyloid protein A and a method for detecting the content of the serum amyloid protein A by adopting the kit for detecting the content of the serum amyloid protein A. By adopting the kit and the method disclosed by the invention, the content of the serum amyloid protein A can be detected easily and rapidly.

Description

technical field [0001] The invention belongs to the technical field of detecting serum amyloid A content, and in particular relates to a kit, method and application for detecting serum amyloid A content. Background technique [0002] Serum amyloid A (SAA) is a highly heterogeneous protein representing a family with a relative molecular mass of 12,000. The SAA gene is located on chromosome 11, and the complete amino acid sequence of human SAA has been obtained. The expression of its mRNAs and proteins in vertebrates are highly conserved, indicating that it has important biological functions. SAA is mainly produced by hepatocytes, but extrahepatic cells have constitutional expression of SAA. The SAA family includes different expression forms of apolipoproteins, acute phase proteins A (A-SAAs) and structural SAAs (C-SAAs). A-SAAs are the main acute-response proteins, and their concentrations can increase as much as 1000-fold during inflammatory responses. In contrast, C-SAAs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/546
CPCG01N33/68G01N33/54313G01N2333/47
Inventor 曹慈崔晓辰夏德习王庆国宿明明
Owner 潍坊三维生物工程集团有限公司
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