Apolipoprotein C3 detecting kit and detecting method for apolipoprotein C3 by adopting same

A technology of apolipoprotein and kit, which is applied in the field of medical immunological in vitro diagnosis, can solve the problems of cumbersome enzyme-linked immunoassay assay process and low degree of automation, and achieve high accuracy, improved detection efficiency, and easy industrialization

Active Publication Date: 2013-05-01
潍坊三维生物工程集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The enzyme-linked immunoassay assay process is relatively cumbersome, and the degree of automation is not high. It needs to be equipped with a variety of special equipment, and batch detection and analysis cannot be directly performed on an automatic biochemical analyzer.

Method used

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  • Apolipoprotein C3 detecting kit and detecting method for apolipoprotein C3 by adopting same
  • Apolipoprotein C3 detecting kit and detecting method for apolipoprotein C3 by adopting same
  • Apolipoprotein C3 detecting kit and detecting method for apolipoprotein C3 by adopting same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Reagent R1:

[0043]

[0044] Reagent R2:

[0045]

[0046]

[0047] Add the corresponding amount of apolipoprotein C3 antigen to the above calibrator solution according to the required concentration of the calibrator to prepare the apolipoprotein C3 calibrator.

[0048] In this example, five reference calibration products with different concentrations were prepared, namely: 0 mg / L, 45 mg / L, 90 mg / L, 120 mg / L, and 180 mg / L. Then use a 0.22 μm filter membrane to filter and sterilize, and store at 2-8°C.

[0049] The Hitachi 7080 automatic biochemical analyzer was used for detection and analysis. Determination method such as figure 1 shown. Use the apolipoprotein C3 calibrator to fit the standard curve through the built-in logit-log (4p) curve fitting mode on the automatic biochemical analyzer, the standard curve is as follows figure 2 As shown; add reagent R1 to the sample to be tested, the sample and reagent R1 volumes are 6 μL and 210 μL respectively, in...

Embodiment 2

[0051] Reagent R1:

[0052]

[0053] Reagent R2:

[0054]

[0055]

[0056] Calibrator:

[0057]

[0058] In this example, a high-concentration single-point calibrator was selected, and the concentration of apolipoprotein C3 antigen was 180 mg / L, and then sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C. When in use, it is diluted with normal saline to form 5 reference calibration products with different concentrations: 0mg / L, 45mg / L, 90mg / L, 120mg / L, 180mg / L.

[0059] The Hitachi 7080 automatic biochemical analyzer was used for detection and analysis. Assay method is the same as in Example 1.

Embodiment 3

[0061] Reagent R1:

[0062]

[0063] Reagent R2:

[0064]

[0065]

[0066] Calibrator:

[0067]

[0068] Add the corresponding amount of apolipoprotein C3 antigen to the above calibrator solution according to the required concentration of the calibrator to prepare the apolipoprotein C3 calibrator.

[0069] In this example, five reference calibration products with different concentrations were prepared, namely: 0 mg / L, 45 mg / L, 90 mg / L, 120 mg / L, and 180 mg / L. Then use a 0.22 μm filter membrane to filter and sterilize, and store at 2-8°C.

[0070] The Hitachi 7080 automatic biochemical analyzer was used for detection and analysis. Assay method is the same as in Example 1.

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Abstract

The invention discloses an apolipoprotein C3 detecting kit, comprising a reagent R1 consisting of a buffer solution, a surfactant, a stabilizer, electrolyte, a macromolecular promoter and a preservative, with the pH of 6-9, a reagent R2 consisting of a buffer solution, an anti-human apolipoprotein C3, a stabilizer, electrolyte and a preservative, with the pH of 6-9, and a calibrator consisting of a buffer solution, an apolipoprotein C3 antigen, a stabilizer, a preservative and antioxidant. The invention further discloses method for detecting an apolipoprotein C3 by using the apolipoprotein C3 detecting kit. The apolipoprotein C3 detecting kit is easy to operate, high in detection speed and wide in detection range; requirements on quick and high-throughput sample detection in clinic can be met; and the detection efficiency is obviously improved.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis of medical immunity, in particular to a kit for detecting apolipoprotein C3 and a detection method realized by using the kit. Background technique [0002] Apolipoprotein C3 mainly exists in very low density lipoprotein, high density lipoprotein and chylomicrons. The full length of the apolipoprotein C3 gene is about 3.1kb, located in the long arm of chromosome 11 within the apolipoprotein A I / C III / A IV gene cluster, and expressed in the liver and small intestine. [0003] Apolipoprotein C3 has the function of inhibiting lipoprotein lipase and liver cell membrane apolipoprotein E receptor, and plays an important role in regulating the catabolism of very low-density lipoprotein and chylomicrons. Severe hypertriglyceridemia is often accompanied by a significant increase in the content of apolipoprotein C3. Therefore, the determination of serum apolipoprotein C3 content is of great significance t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 宿明明王庆国王爱龙
Owner 潍坊三维生物工程集团有限公司
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