Quick quantitative detecting device and method for simultaneously detecting human brain natriuretic peptides and N-terminal pro-brain natriuretic peptides

A detection device and quantitative detection technology, which is applied in the field of medical clinical diagnosis, can solve the problems of simultaneous detection of rapid detection reagents/devices, etc., and achieve the effects of fast response, accurate results, and easy operation

Active Publication Date: 2014-01-22
天津中新科炬生物制药股份有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] At present, both BNP and NT-ProBNP detection reagents are used clinically, but ther

Method used

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  • Quick quantitative detecting device and method for simultaneously detecting human brain natriuretic peptides and N-terminal pro-brain natriuretic peptides
  • Quick quantitative detecting device and method for simultaneously detecting human brain natriuretic peptides and N-terminal pro-brain natriuretic peptides

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Experimental program
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Embodiment

[0019] Example: Preparation and application of a rapid quantitative detection device for human BNP and N-terminal BNP precursor

[0020] 1 main material

[0021] 1.1 Biological material BNP and NT-ProBNP paired antibody, purchased from Finland HYTEST company; mouse anti-human IgG antibody, goat anti-mouse IgG: self-made; chloroauric acid: product of Sigma company; NC membrane: product of Sartorius company; hydrolyzed casein, poly Ethylene glycol PEG20000: a product of Sigma. Other commonly used reagents are analytical reagents.

[0022] 1.2 The company obtained 120 clinical samples in related hospitals, including 60 serum samples from patients diagnosed with heart failure. The detection value of BNP was from 0.2-8ng / mL, and the detection value of NT-ProBNP was from 0.2-20ng / mL. 60 normal human serum samples, BNP and NT-ProBNP values ​​were not detected.

[0023] 1.3 Duplex clamping shell: designed by our company, and related companies produce and provide according to requir...

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Abstract

A quick quantitative detecting device and method for simultaneously detecting human brain natriuretic peptides and N-terminal pro-brain natriuretic peptides are characterized in that the device is composed of human brain natriuretic peptide (BNP) test paper, N-terminal pro-brain natriuretic peptide (NT-ProBNP) test paper, a double link clamping shell and an immune chromatography result interpretation recorder, the BNP test paper and the NT-ProBNP test paper are mutually independent, and corresponding standard curves and judging methods are arranged in the immune chromatography result interpretation recorder. The immune chromatography result interpretation recorder is an optical detection system and is used for quantitative judging of detection results. The detection range for the BNP and the detection range for the NT-ProBNP are 0.1-10ng/mL and 0.2-20ng/mL respectively. The detection is finished in 15-20 minutes. The quick quantitative detecting device and method have the advantages of detecting in a combined mode, and being simple and convenient to operate, quick in response, accurate in result, suitable for on-site detection and the like, and suitable for requirements of clinic for more accurate, simpler and more convenient detection.

Description

technical field [0001] The patent of the present invention relates to the technical field of medical clinical diagnosis, in particular to a rapid quantitative detection device and detection method for simultaneous detection of human brain natriuretic peptide and N-terminal brain natriuretic peptide precursor prepared by an immunocolloidal gold rapid detection method. Background technique [0002] Brain natriuretic peptide (BNP) was first isolated from pig brain in 1988 by Japanese scholar Sudoh et al. Human brain natriuretic peptide (BNP) is widely distributed in brain, heart, lung, digestive tract, genitourinary tract and other tissues, among which the heart has the highest level. Cardiomyocytes do not directly synthesize BNP, but synthesize pro-BNP first. Pro-BNP has 108 amino acids, which will be decomposed in the blood. The decomposed fragments are 76 amino acids at the N-terminal, namely NT-ProBNP, and the remaining 32 amino acids, namely BNP. [0003] Studies have fo...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/532G01N33/551G01N33/558G01N33/68G01N2800/325
Inventor 李洲杨发青周鸿锐
Owner 天津中新科炬生物制药股份有限公司
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