Quick quantitative detecting device and method for simultaneously detecting human brain natriuretic peptides and N-terminal pro-brain natriuretic peptides
A detection device and quantitative detection technology, which is applied in the field of medical clinical diagnosis, can solve the problems of simultaneous detection of rapid detection reagents/devices, etc., and achieve the effects of fast response, accurate results, and easy operation
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[0019] Example: Preparation and application of a rapid quantitative detection device for human BNP and N-terminal BNP precursor
[0020] 1 main material
[0021] 1.1 Biological material BNP and NT-ProBNP paired antibody, purchased from Finland HYTEST company; mouse anti-human IgG antibody, goat anti-mouse IgG: self-made; chloroauric acid: product of Sigma company; NC membrane: product of Sartorius company; hydrolyzed casein, poly Ethylene glycol PEG20000: a product of Sigma. Other commonly used reagents are analytical reagents.
[0022] 1.2 The company obtained 120 clinical samples in related hospitals, including 60 serum samples from patients diagnosed with heart failure. The detection value of BNP was from 0.2-8ng / mL, and the detection value of NT-ProBNP was from 0.2-20ng / mL. 60 normal human serum samples, BNP and NT-ProBNP values were not detected.
[0023] 1.3 Duplex clamping shell: designed by our company, and related companies produce and provide according to requir...
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