281results about How to "Suitable for on-site testing" patented technology

An off-resonance photoacoustic spectrometric detection and analysis device consists of a photoacoustic spectrometric module (8). The photoacoustic spectrometric module (8) comprises a light source module (2-1), a photoacoustic signal generation module (2-2), an optical power measurement module (2-3) and a fault diagnostic analysis module (2-4). Optical signals generated by an infrared incoherence light source in the light source module (2-1) are focused by an ellipsoidal reflector to form a focused light beam, the focused light beam is modulated into light of specific frequency and wavelength by chopping blades of an optical chopper and optical filters and then transmitted to the photoacoustic signal generation module (2-2), and the fault diagnostic analysis module (2-4) calculates each gas concentration according to to-be-tested gas photoacoustic signals generated by irradiation, diagnoses faults and alarms. The off-resonance photoacoustic spectrometric detection and analysis device can be used for quantitative detection of gases such as SF6, CF4, SO2F2, SOF2, SO2, SF4 and H2O and is applicable to onsite online monitoring.

The invention discloses a method for rapidly detecting polybrominated diphenyl ether residue in a sample, particularly in textiles. The method is characterized by comprising the following steps: (1) the sample is crushed, dipped in base solution and ultrasonically extracted in water bath, and solid phase microextraction is employed to concentrate a target compound; and (2) polybrominated diphenyl ether is qualitatively and quantitatively determined by gas chromatography-mass spectrometry after thermal desorption. The method helps effectively solve the problem of matrix effect that fabric fibers have no solvent extraction, obtains a satisfactory result for the analysis of a selected target compound, has the advantages of low organic solvent consumption, rapid extraction speed, less interference from impurities, high sample recovery rate and the like, and can be applicable to rapidly detecting polybrominated diphenyl ether flame retardant residue in textiles.

The invention belongs to the field of nucleic acid detection and discloses a method for semi-quantitatively detecting pathogenic nucleic acid by adding internal control nucleic acid. Corresponding internal control is added in the whole process of extracting and amplifying target nucleic acid and testing by using a test paper, so that the internal control and a target segment are parallelly operated, and the semi-quantitative detection is performed finally through color development and intensity contrast of three strips, namely a detection line, an internal control line and a quality control line on the test paper. In the method, in the whole process of processing the target nucleic acid, the corresponding internal control is taken as a positive contrast, and false negative results due to links such as extraction, amplification or sample application errors are avoided in the processing of detecting by using the test paper. Meanwhile, by comparing color development intensity of the internal control line and a sample line and introducing the semi-quantitative function on the basis of the qualitative function of the immunochromatographic test paper to estimate the copy number of tested samples, the detection results are more detailed, accurate and reliable. The method has the advantages of convenient and quick operation and capacity of meeting the actual clinical requirement.

A combined device for rapidly and quantitatively detecting multiple tumor markers is characterized by being composed of 3 to 8 pieces of independent tumor marker test paper such as AFP, CEA, PSA, CA125, CA153, CA199, CA242 and NSE, a clamping shell special for the 3 to 8 pieces of test paper, and an immune layer result analysis and judgment recorder with built-in corresponding standard curves and the judgment method. The test paper can be combined to form the detection device with the 3 to 8 pieces of test paper according to the specific requirements. The standard curves and the judgment method for the 8 pieces of test paper are built in the immune layer result analysis and judgment recorder and used for conducting quantitative judgment on test results, and forecasting of various tumor occurrence conditions can be conducted on different types of combination of the test results through the judgment method. Detection is completed within 15 minutes to 20 minutes. The combined device for rapidly and quantitatively detecting the multiple tumor markers has the advantages of being combined in detection, easy and convenient to operate, rapid in response, accurate in result, suitable for on-site test and the like, and the requirements for more accurate, easier and move convenient clinical detection are met.

The invention relates to an undetached measurement device and method for transmission efficiency of an automotive transmission system. The device has the advantages of accurate measurement, high efficiency, easy manufacture, and high practical value. The technical scheme of the invention is that: the device consists of a first rotating drum, a second rotating drum, an eddy current dynamometer arm, an eddy current dynamometer, a rotating drum center shaft, and a coupler, wherein the undetached measurement device for the transmission efficiency of the automotive transmission system also comprises an eddy current machine arm dynamometer, an automotive driving force dynamometer, and a speed regulating motor; an automotive rear hook is connected with the automotive driving force dynamometer through a pull rod; and the automotive driving force dynamometer is fixed on a fixed pile. The device and the method have the advantages of solving the problems of undetached measurement of automobile engine dynamic property and economical efficiency, and realizing the rapid detection of the automobile engine dynamic property and economical efficiency detection station. By combining modern computer technology, the device and the method can realize rapid display of the automobile engine dynamic property and economical efficiency detection, and print the results on the spot.

The invention provides a pesticide residue detection micro-fluidic chip with a pre-stored reaction reagent. The pesticide residue detection micro-fluidic chip is characterized by comprising a micro-fluidic channel, a liquid sac pool, an extracting solution sac, a sample pool, a sample pool cover, a plurality of reagent pools and bio-reagents stored in the reagent pools. During detection, a to-be-detected sample just needs to be put into the sample pool of the chip, a reagent solution such as an extracting solution, an enzyme liquid, a color developing agent solution or a primer solution does not need to be prepared. The chip is concise to operate and suitable for field detection of pesticide residues and has an extremely important application value.

The invention relates to a method of applying Rayleigh waves in non-linear ultrasonic evaluation of surface damage of a metal material. The method comprises the following steps: 1) excitation and reception of Rayleigh waves; 2) detection of reliability of a testing system; 3) measurement of non-linear coefficients when the surface of a test piece has damage of different degrees; 4) repeatable operation of test. Compared to the method of using body waves for ultrasonic nondestructive test, the non-linear ultrasonic evaluation method provided in the invention has the characteristic that Rayleigh surface waves are especially suitable for measurement of non-linear coefficients of large-scale complex plate structures. According to the invention, it is only needed to carry out excitation and measurement of supersonic waves at one side of a structure during measuring, which enables measuring to be simple and easy; the Rayleigh surface waves have advantages favorable for measuring, e.g., concentration of energy on the surface of a structure, a long propagation distance, etc.; operation is simple and easy, so the method is especially suitable for on-site detection of structural elements; effective evaluation of early damage and degeneration of mechanical properties of a material structure can be realized by using the non-linear ultrasonic method.

The invention is applicable to the field of analytical chemistry and in particular relates to an identification method of ganoderma lucidum spore oil. The method is used for distinguishing the ganoderma lucidum spore oil from rapeseed oil, soybean oil, corn oil, sunflower seed oil, peanut oil and olive oil, and comprises the following steps: carrying out laser spectrum scanning on a sample to be detected to obtain a characteristic Raman spectrogram of the sample; comparing the Raman spectrogram with standard conditions, wherein the standard conditions are as follows: a displacement value at a first part is 1300+/-3cm<1> and a displacement value at a second part is 1262+/-3cm<1>, the Raman spectrum intensity values of the first part and the second part are more than 200, and the ratio of the Raman spectrum intensity values of the first part and the second part, and the ratio of signal peak areas of the first part and the second part are more than 2; and determining the content of ergosterol on a sample meeting the standards, wherein the sample with the content of the ergosterol of 0.10-2.79mg/g is the ganoderma lucidum spore oil. The identification method provided by the invention is simple and rapid, and has a wide application prospect in the aspect of rapid identification of the ganoderma lucidum spore oil and other common vegetable oil.

The invention relates to a device and a method for fruit and vegetable pesticide residues based on a laser Raman spectrometer. With the development of agricultural technology, various pesticides are used for prevention of fruit and vegetable pests in order to meet people's requirements in daily life by efficiently producing the fruits and vegetables with high yield, thus pesticide residues are formed on the surfaces of the fruits and vegetables unavoidably. The device for fruit and vegetable pesticide residues based on the laser Raman spectrometer comprises a display screen, a RS232 cable, a processor, a laser device, optical fibers, clamp nuts, a lens, a sample support, a probe housing, a reflective mirror, an optical fiber coupler, a pinhole, a focus lens, a collimating lens, optical gratings, a detector, a cut-off optical filter and a data line which are all fixed on the casing of the device. The device for fruit and vegetable pesticide residues based on the laser Raman spectrometer has the advantages of being compact in structure, low in power dissipation, high in stability, convenient to carry, and suitable for field detections. During detection, no movement of the device is needed, detection results are not affected by external vibrations, and the detection precision and speed are high.

The invention provides ketamine-collaurum test paper for detection of saliva. The ketamine-collaurum test paper comprises a reaction film and a gold mark cushion, wherein according to the reaction film, a reaction line and a quality control line are formed by respectively coating ketamine and quality-control secondary antibodies IgG (immunoglobulin G) connected with carrier protein on a nitrocellulose film (NC film); the gold mark cushion is coated with ketamine monoclonal antibodies marked with collaurum; and a film chromatography competitive inhibition method is adopted to detect the ketamine in a saliva sample. The test paper can be used for effectively monitoring the ketamine in saliva, the detection specificity is strong, the repeatability is good, the sensitivity is high, and the lowest detection quantity reaches 50ng/ml. The test paper is simple to operate, does not need special instruments and equipment, does not need professional training and has clear and obvious results, thus being suitable for field detection.

The invention discloses an LAMP primer group and an LAMP detection kit for staphylococcus aureus and a use method of the detection kit. The LAMP primer group for staphylococcus aureus comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. According to the LAMP primer group, the LAMP detection kit and the use method, loop-mediated isothermal amplification primers are designed according to the sequences of the conservative regions of the heat-resistant nuclease genes (nuc) of staphylococcus aureus, the specific regions of target genes are amplified by adopting the LAMP technology, thus the rapid detection for staphylococcus aureus is realized from the molecular level, and an effective and rapid nucleic acid screening detection method is provided for detecting staphylococcus aureus.

The invention provides a direct determination method for the mercury content in natural gas. The determination method comprises the following steps of: (1) connecting determination devices, wherein the determination devices comprise a gasbag filled with the natural gas, a first three-way valve, a second three-way valve, a three-way pipe, a mercury filter and a mercury detector; and (2) firstly introducing the natural gas into the mercury detector and detecting to obtain a detected value C1, filtering out the mercury from the natural gas by using the mercury filter, then, feeding the filtered natural gas into the mercury detector and detecting to obtain a detected value C2, and obtaining the mercury content C in the natural gas according to an equation, i.e. C= C1-C2. The determination method provided by the invention has the advantages of high accuracy in obtained detection result, good repeatability, strong applicability, and no influence arising from oil content and moisture content, and is applicable to natural gases of various types, such as wellhead natural gas and the feed gas and product gas of a treatment plant.

The invention relates to a detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on a micro-fluidic chip and application of the detection method. The method is characterized by taking the micro-fluidic chip with a plurality of pipelines as a reaction platform and detecting by using a chemical luminescent signal so as to independently or simultaneously detect the serum specific antibodies IgM of mycoplasma pneumonia and influenza viruses. As the micro-fluidic chip is combined with chemiluminiscence, the detection method integrates the advantages of rapidness, high efficiency and small sample amount of the micro-fluidic chip and high specificity of chemiluminiscence, also has the advantages of simplicity in operation, low cost, rapidness and the like, is a multi-target, real-time and high-sensitivity detection method and can be applied to rapid diagnosis of pneumonia and influenza.

The invention discloses a LAMP detection primer, kit and detection method for staphylococcus aureus containing an internal standard. The primer is shown as SEQ ID NO 1-SEQ ID NO 12, and comprises a detection primer group and an internal standard primer group; and the detection kit comprises a primer group, a reaction solution, a DNA polymerase, positive control and an internal standard. According to the detection method, a sample to be detected is extracted, and a sample template is subjected to amplification for 45-60 minutes at the temperature of 63-65 DEG C, so that whether the sample to be detected contains the staphylococcus aureus can be detected, and whether a false negative detection result exists can be judged according to the fact that whether the internal standard primer group is subjected to amplification. The LAMP detection primer has the advantages of high rapidness, high efficiency, easy and convenient operation, high specificity, high sensitivity, suitableness for field detection and the like; and moreover, false negative detection can be effectively avoided, and the LAMP detection primer is suitable for popularization and application.

The invention dislcoses an RAA (recombinase-aid amplification) constant-temperature fluorescence detection method and a detection kit for EHP (enterocytozoon hepatopenaei). The detection kit comprisesa forward primer SEQ ID NO. 1, a reverse primer SEQ ID NO. 2, a specific fluorescence probe SEQ ID NO. 3, reaction liquid, recombinant polymerase and a reference substance. The kit has the advantagesthat the specificity is strong; the detection sensitivity is high, and can reach 100fg/mu L; high accuracy and reliability are achieved; the operation is simple and rapid, and the kit is suitable forfield detection and has wide application scenarios.

The invention provides application of a cholesterol molecule imprinted membrane sensing electrode in detecting cholesterol content in blood. Specific steps in the method are as follows: (1) treating a blood sample, that is, standing the blood sample at room temperature and carrying out centrifugation to extract serum if the blood sample is obtained by drawing blood from a person who is on an empty stomach, or carrying out anticoagulation by using heparin or EDTA (ethylene diamine tetraacetic acid) if the blood sample is blood plasma; (2) dropwise adding the blood sample obtained in step (1) onto the cholesterol molecule imprinted membrane sensing electrode, inserting the cholesterol molecule imprinted membrane sensing electrode into an amperometric sensor for determination, and recording readings. When used for detecting cholesterol content in blood, the molecule imprinted membrane sensing electrode has the advantages of low cost, high sensitivity, fast analysis speed, capacity of reducing sampling amount and avoiding tedious pretreatment of blood samples, etc.