The invention belongs to the technical field of immunological detection, and more specifically relates to a kit used for quantitative determination anti-nucleosome antibody Ig G via magnetic micro particle chemiluminiscence, and a preparation method and a detection method thereof. The kit comprises an anti-nucleosome antibody Ig G calibration material, an anti-nucleosome antibody Ig G reagent 1, an anti-nucleosome antibody Ig G reagent 2, an anti-nucleosome antibody Ig G magnetic separation reagent, an anti-nucleosome antibody Ig G quality control product, and a cleaning solution. The invention also discloses the preparation method and the detection method of the kit. The detection method is invented based on conventional membrane strip immunization and enzyme linked immunosorbent assay, sensitivity and linearity range are increased 10<3> to 10<5>, quantitative determination is realized, sensitivity, specificity, and accuracy are high, cost is low, operation is simple and convenient, result judgment is objective, fully automatic application is realized via combination with an automatic chemicaluminescence immunity analyzer, and application prospect is promising.