Efficient quintuplet primer for detecting shrimp diseases and kit

A kit and high-efficiency technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of lack of hot-start enzymes, insufficient specificity, high cost, etc., and achieve rapid on-site The effect of detection, avoiding non-specific reactions, and easy operation
CN112981006APending Publication Date: 2021-06-18宁波爱基因科技有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
宁波爱基因科技有限公司
Publication Date
2021-06-18

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Abstract

The invention discloses an efficient quintuplet primer for detecting shrimp diseases and a kit. According to the primer and the kit, an improved LAMP technology serves as a gene amplification reaction principle, and gold nanoparticles are added into a reaction system to adsorb ssDNA and protease and inhibit nonspecific reactions during heating, thus, the aim of warm start is achieved, and nonspecific reactions during heating-up are avoided; and by combining the improved LAMP technology and a microfluidic chip technology, an accurate detection result can be presented rapidly, and the aim of joint detection on the same sample with a plurality of different indexes is achieved; meanwhile, reaction reagents are pre-buried into a microfluidic chip, five-index detection on one same is achieved, a user only needs to add a sample, and operations are simple and convenient; and the instrument is equipped with lithium batteries, so that on-site rapid detection is facilitated.
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Description

technical field

[0001] The invention belongs to the technical field of gene detection, and in particular relates to a highly efficient five-way detection method for shrimp diseases (prawn infectious subcutaneous and hematopoietic necrosis virus (infectious type), prawn infectious subcutaneous and hematopoietic tissue necrosis virus (general type), yellow head virus , Stealing wild Tamura virus, Taura virus) primers and kits. Background technique

[0002] Infectious hypodermal and hematopoietic necrosis virus (IHHNV), also known as Litopenaeus stylirostris densovirus and decapod densovirus, is the smallest virus among known prawn viruses, with a particle size of 22nm. Non-encapsulated icosahedron, linear single-stranded DNA, about 3.9kb in length, containing 3 open reading frames. IHHNV genotypes are divided into infectious and non-infectious types, of which infectious types include types 1 and 2; non-infectious types include types 3A and 3B. IHHNV is widely prevalent in th...

Claims

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