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A kind of immunochromatographic reagent composition and detection method for canine coronavirus

An immunochromatographic detection and immunochromatography technology, applied in measurement devices, analytical materials, biological material analysis, etc., can solve the problem that EDTA hemoglobin does not have sufficient effect, shorten the detection time, inhibit non-specific reactions, and judge the results. accurate effect

Active Publication Date: 2019-01-04
江苏雷森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, EDTA does not have a sufficient effect on the stabilization of hemoglobin contained in samples such as stool, and the stabilization effect obtained by using a water-soluble metal complex of a transition metal ion is higher than that obtained by using only EDTA

Method used

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  • A kind of immunochromatographic reagent composition and detection method for canine coronavirus
  • A kind of immunochromatographic reagent composition and detection method for canine coronavirus
  • A kind of immunochromatographic reagent composition and detection method for canine coronavirus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Reagent composition for immunochromatography:

[0038]1.1 There is no special requirement for the buffer in the reagent composition for immunochromatography. The buffer types include: acetate buffer, phosphate buffer, boric acid buffer, Tris hydrochloric acid buffer, etc., preferably Tris hydrochloric acid buffer. The concentration is in the range of 0.01-250 mM, preferably in the range of 10-200 mM, more preferably in the range of 30-180 mM. If the concentration is lower than 0.01 mM, the buffering effect is insufficient. When the concentration is above 250mM, the concentration is higher than necessary, resulting in waste.

[0039] 1.2 The chelating agent which is one of the components of the reagent composition for immunochromatography of the present invention is not particularly limited as long as it can be used as a ligand having a plurality of ligands. As a chelating agent in the present invention, a compound containing amino and carboxylic acid functional groups...

Embodiment 2

[0042] Immunoassay test strip device:

[0043] 2.1 Marking pads and sample pads are usually made of glass fiber membrane. The marker pad contains the combination of colloidal gold particles and monoclonal antibody or polyclonal antibody fragments thereof.

[0044] 2.2 Chromatography medium mainly adopts nitrocellulose membrane. The chromatographic medium is marked with monoclonal or polyclonal antibodies for identifying antigens, and secondary antibodies for identifying markers.

[0045] 2.3 The absorbent pad can be made of filter paper.

[0046] 2.4 One side of the back plate is coated with adhesive or adhesive tape to make it sticky, and the sample pad part, marker part, chromatographic medium part and water absorption part are attached to the adhesive surface.

[0047] 2.5 Brief description of judgment principle

[0048] After dipping the end of the sample pad (treated with the immunoreagent composition) into the sample, the sample solution swims from bottom to top alon...

Embodiment 3

[0050] The preparation method of canine coronavirus colloidal gold immunochromatographic detection test strip is as follows:

[0051] 3.1 Preparation of markers

[0052] Add 0.1ml of canine coronavirus antigen monoclonal antibody [phosphate buffer (pH7.4 diluted to 1mg / ml)] in 1ml of colloidal gold solution, let stand at room temperature for 1 minute, then add 100μl of 10% bovine serum For protein (BSA), centrifuge at 8000rpm for 15 minutes after stirring well. After removing the supernatant, the above-mentioned phosphate buffer solution was added to prepare a labeling reagent solution.

[0053] 3.2 Preparation of the judgment part on the chromatographic membrane

[0054] Dilute the monoclonal antibody to canine coronavirus antigen to 1.0mg / ml with phosphate buffer (pH7.4), dilute the secondary antibody to 1.0mg / ml, and draw the two to the chromatographic membrane at the same time by spraying a gold-spraying membrane device Serve, dry and set aside.

[0055] 3.3 Assembly o...

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Abstract

The invention provides a reagent composition or to-be-tested substance treating fluid capable of inhibiting non-specific reactions compared with conventional immunochromatographic detection technology for a canine coronavirus. The invention also provides a high-performance high-sensitivity immunochromatographic detection apparatus and detection method using the reagent composition. When an immunochromatographic test strip is used for detecting the canine coronavirus in a sample, the sensitivity of immunoreactions can be improved and non-specificity of the immunoreactions can be lowered by using the reagent composition containing weak a diluent, a chelating agent, a surfactant and the like or a diluted solution of a to-be-tested substance for immunochromatography.

Description

technical field [0001] The invention relates to a reagent composition for immunochromatography or a sample processing solution for detecting canine coronavirus, which improves sensitivity and can suppress non-specific reactions. The present invention further relates to an immunochromatographic detection method capable of suppressing non-specific reactions and measuring analytes quickly, easily and with high precision. Background technique [0002] The causative agent of canine coronavirus disease is canine coronavirus (Canine Corona Virus, CCV). It is clinically characterized by gastroenteritis of varying severity and clinically fatal watery diarrhea. Sudden onset, depression, loss of appetite, vomiting, discharge of foul-smelling soft and mucous feces. Vomiting often lasts for several days and does not subside until diarrhea develops. Afterwards, the feces range from semen-like, semi-pasty to watery, orange or green, and contain mucus and varying amounts of blood. Rapid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/56983G01N2333/165
Inventor 秦雷陈芳张旭东谭影影秦允震黄蓓蓓
Owner 江苏雷森生物科技有限公司
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