189 results about "Non specificity" patented technology

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

The invention provides a real-time quantitative detection reagent and a method of time-resolved fluorescence immune chromatography. The invention provides emulsion particles containing lanthanide elements coated by a gel, an antibody compound formed by the emulsion particles and an antibody coupled to the emulsion particles, a detection strip containing the antibody compound and a kit containing the detection strip, and applications thereof. The emulsion particles, the antibody compound and the kit can greatly eliminate non-specificity combination, and enables signal-to-noise ratio of detection signals to be increased significantly. The stability can be improved by coating the emulsion particles with the gel. The detection method has the advantages of high speed, high sensitivity, high specificity, etc.

Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.

The invention discloses a test strip for fluorescence immunochromatography of myeloperoxidase. The test strip comprises a base plate, a sample pad, a binding pad, a nitrocellulose membrane and an absorbent pad, and the sample pad, the binding pad, the nitrocellulose membrane and the absorbent pad are assembled on the base plate in a sequential overlapping manner, wherein the absorbent pad and thebinding pad are respectively pressed on two ends of the nitrocellulose membrane in an overlapping manner, and a detection area is formed on the surface of the nitrocellulose membrane; the sample pad is pressed on the binding pad in an overlapping manner, and a myeloperoxidase antibody-fluorescent microsphere compound is immobilized on the binding pad; and the nitrocellulose membrane in the detection area is coated with a detection line formed by a monoclonal antibody for recognizing another epitope of myeloperoxidase and a control line formed by a goat anti-mouse IgG polyclonal antibody. The test strip has the advantages of high sensitivity, high stability, realization of the detection linearity being 3.125-600 ng/ml, no non-specificity, short detection time of 5 min, realization of bedside quick test, and great improvement of the clinical diagnosis efficiency.

The invention discloses a fluorescence immunochromatographic assay method for quantitatively detecting hFABP (heart fatty acid binding protein) and a kit for quantitatively detecting the same. The fluorescence immunochromatographic assay method for quantitatively detecting the hFABP realizes quantitative fluorescence detection on the basis of optimizing components of a test strip by the aid of excellent fluorescent characteristics of quantum dots and by means of combining bicolor labeling technique and immunochromatographic assay. Compared with a conventional colloidal gold immunochromatographic assay method, the fluorescence immunochromatographic assay method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The kit is used for quantitatively detecting the hFABP, can be used for simultaneously detecting whole blood, blood serum and plasma samples, serves as a simple, accurate, specific and inexpensive detecting tool for early screening and prognosis evaluation of acute myocardial infarction, is applicable to hospitals at all levels, and is particularly beneficial to wide popularization in primary hospitals and clinics.

The invention discloses a fluorescence immunochromatographic assay and kit for quantitatively detecting acute myocardial infarction marker, namely, N-terminal pro brain natriuretic peptide (NT-proBNP). The fluorescence immunochromatographic assay for quantitatively detecting the NT-proBNP realizes fluorescence quantitative detection on the basis of optimizing various constituent parts of test paper by using excellent fluorescence characteristic of quantum dots and combining a bicolor marking technology and an immunochromatographic technology. Compared with the conventional colloidal gold immunochromatographic assay, the fluorescence immunochromatographic assay disclosed by the invention has the advantages of good marking stability, low non-specificity, high sensitivity, wide linear range and quantifying accuracy. The fluorescence immunochromatographic kit disclosed by the invention is used for carrying out quantitative detection on the NT-proBNP and detecting whole blood, blood serum and blood plasma samples, can provide reference for diagnosis of cardiovascular and cerebrovascular diseases and can be widely applied to primary hospitals and clinics.

The invention discloses a personalized medication gene detection kit and application. The personalized medication gene detection kit is suitable for multiple kinds of sample types including dried blood spots, whole blood and buccal swabs, information of all medicine gene polymorphic sites can be acquired at once, the sequencing effective rate is 50% or above, the sequencing cover degree is 100%, the average sequencing depth exceeds 3000 X, the library building success rate is larger than or equal to 98.5%, and the medicine gene polymorphic site detection accuracy is larger than or equal to 99.9%; a primer group is high in specificity and little in nonspecific amplification, interference among primers can be well overcome, under the situation of up to 186 primer sequences, one-tube multiplePCR reaction is achieved, moreover, amplicon is high in uniformity, and under the situation of large amplification area GC content difference, overall effective amplification can be conducted. The detection kit, the primer group and a library building method can be applied to personalized medication gene detection, and guidance is provided for personalized medication.

The invention discloses a method for improving overwintering survival rate and qualities of oreochromis niloticus fries. Before overwintering, feeds with 12% to 14% fat level are fed to the fries, and soybean oil and fish oil are blent in a proportion of 4:1 to form fat sources. Fatty acids--linoleic acids and linolenic acids which are necessary to growth of oreochromis niloticus are mainly increased so as to improve energy reserve. Low-temperature stress resistance of the oreochromis niloticus can be improved by low temperature acclimatization and the low temperature adaptability is improved. During overwintering, feeds with 1.5% to 2% compound Chinese herbal medicine are fed to the fries, so that damages of the low-temperature stress to fish bodies can be effectively relieved, the non-specificity immunity of the fish bodies is improved, and the overwintering survival rate and fry qualities are improved. Meanwhile, according to the above three steps, material selection and facility configuration are easy, the price is relatively low, processes are simple, traditional problems that overwintering costs are high, the survival rate of the fries is low, and qualities of the fries are poor are excellently solved, and environment-friendly and physically beneficial breeding requirements can be met.

The invention relates to an artificial particle feed for juvenile turbot. The artificial particle feed is characterized by being prepared from whitefish powder, euphausiid powder, scallop powder, beer yeast, starch, cod liver oil, yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride, soybean lecithin, schizochytrium limacinum powder, haematococcus pluvialis powder, compound vitamins, compound mineral substances, Chinese herbal medicines and sodium alginate. As the various raw materials are added into the feed, the comprehensiveness of nutrition is guaranteed. An immunopotentiator is added into the feed, so that the non-specificity immunity of fish bodies is enhanced. The feed is green and pollution-free. The schizochytrium limacinum powder and the haematococcus pluvialis powder are added into the feed, and the content of vitamin E is improved, so that the whitening rate of the juvenile fish is effectively reduced. The Chinese herbal medicine components are added, so that the immunity of the juvenile fish can be improved and parasite diseases in a juvenile fish growing phase are prevented from happening.

The invention relates to a water-soluble polymer adsorption material of a coupling cyclodextrin in the field of biomedical materials and an application thereof. The adsorption material comprises a coupling ligand which takes natural polysaccharide with amino or hydroxyl and derivatives thereof or chemosynthesis polymer as carriers and is characterized in that the molecule of the polymer adsorption material has the structure of formula (I). In the formula, R is chosen from natural polysaccharide with amino or hydroxyl and derivatives thereof or chemosynthesis polymer with the viscosity average molecular weight between 6KD and 500KD; X is chosen from C0-C6 alkane chain with O atom, N atom or OH group; CD is chosen from Beta-cyclodextrin or substitutional Beta-cyclodextrin. The adsorption material dissolved into a medical dialyzate can be applied to blood purification treatment for grave hepatitis patients. The adsorption material of the invention has the advantages of removing small molecular protein bound toxin and water soluble toxin molecular at the same time, low treatment cost which is only about one tenth of that of a molecular adsorption recycling system and low non-specificity adsorption of plasma protein.

The invention provides a pig IFN (interferon) gamma-Fc fusion protein optimized according to a silkworm reaction system and a coding gene of the pig IFNgamma-Fc fusion protein and a method of expressing pig IFNgamma-Fc fusion protein by using a silkworm bioreactor, and belongs to the field of a biological genetic engineering. The pig interferon gamma serving as a non-special broad spectrum antiviral biological agent has a wide medicinal prospect in a veterinary drug field; but like most of genetic engineering veterinary drugs, the pig interferon gamma also has the problems such as insufficient output, expensive price, irregular quality, short acting time and the like. A target protein with a high expression efficiency and strong activity is obtained by expressing the pig IFNgamma-Fc fusion protein optimized by using a silkworm expression system. In addition, the acting time of the pig interferon gamma is prolonged by addition of an Fc segment, so that the overall immune regulation effect is enhanced, and purification at a later stage is facilitated, thus a pig IFNgamma-Fc fusion protein preparation produced by using a genetic engineering method is possible, and a condition is also created for developing a novel feed containing the fusion protein.

The invention relates to a soft grain feed for a porket. In the invention, according to the technology explanation book editable fat is adopted as additive raw material and added with mineral matters, premix compound of vitamins, dairy products and expansion grains and solid raw materials are crushed into fine powders; then liquid raw materials such as hot water, lard, bean oil, lactic acid and emulsifier are mixed and blended evenly in sequence; the mixed liquid raw materials are added into the solid raw materials by high pressure injection and mixed evenly with the solid raw material; finally the mixed materials are made into grains in the steamless low temperature. The lard and the emulsifier are added as additives and promote softness and crisp and have good taste of the feeds and adapt the feeds to digestion physiological characteristics of the porket; the adding of dairy products not only guarantees high nutritional concentration but also greatly improves appetite and digestion ability of the porket. The feeds of the invention can improve taste of feeds, appetite of the porket, help quick growth of the porket, prevent atrophy of intestinal villi of the porket when ablactation, reduce stress caused by ablactation, improve non-specificity immunity and guarantee health of the porket.

The invention discloses a lactic dehydrogenase detection kit and a preparation method of the kit. The kit disclosed by the invention consists of a reagent I and a reagent II which are independent each other, wherein the components of the reagent I comprise a biobuffer, a metal ion complex, a lactic dehydrogenase reactive substrate, a surfactant, a lactic dehydrogenase activity activating agent, a preservative and water; and the components of the reagent II comprise a biobuffer, a nicotinamide adenine dinucleotide oxidation state, a surfactant, a preservative and water. The detection kit disclosed by the invention adopts a dual reagent mode to effectively avoid the interference of the nonspecific reaction and synchronously furthest reduce the interference of the sample turbidity, thereby guaranteeing the stable and reliable measurement result; and the detection kit has the advantages of good stability, high precision, wide linear testing range, good repeatability, and strong anti-interference performance and the like. In addition, the detection kit disclosed by the invention does not need to pre-dilute the sample in the detecting process, thereby being convenient for clinical use, simple and fast to operate, and suitable for an automatic biochemical analyzer.

The invention provides a preparation method and application of a new therapeutic vaccine for dendritic tumor cells. The therapeutic tumor vaccine is chemotherapeutic drug induced tumor antigen, wherein, the dendritic cells are loaded and activated. The chemotherapeutic medicament induced tumor antigen contains a plurality of immunostimulation molecules as well as tumor-associated and specific antigen, which can remarkably carry out chemotaxis and activation on immunocytes such as the dendritic cells, T-cells (thymus-dependent lymphocytes) and the like, stimulate the dendritic cells to be mature and express a plurality of cell factors and chemotactic factors, effectively induce immune response reaction with antigenic specificity and non-specificity and strengthen body immune function. The therapeutic vaccine provided by the invention can be used for preventing and treating tumors and has the characteristics of simple preparation process, low cost, strong specificity, obvious curative effect and the like.

The invention discloses a hydrophobic modified choline phosphorylated chitosan self-assembled nano microparticle and a preparation method thereof, belonging to the technical field of nano pharmaceutical preparations. The preparation method of the hydrophobic modified choline phosphorylated chitosan self-assembled nano microparticle comprises the steps of (1) making 6-O-triphenyl methylated chitosan obtained from chitosan modification react with disubstituted choline phosphonate to obtain a choline phosphorylated chitosan derivative; (2) introducing a hydrophobic group to the choline phosphorylated chitosan derivative through an N-acylation reaction to obtain a hydrophobic modified choline phosphorylated chitosan derivative; and (3) dispersing the hydrophobic modified choline phosphorylated chitosan derivative in a water solution, stirring and implementing ultrasonic treatment to obtain the hydrophobic modified choline phosphorylated chitosan self-assembled nano microparticle. The prepared hydrophobic modified choline phosphorylated chitosan self-assembled nano microparticle can inhibit non-specificity protein adsorption, and is easy to enter into cells, thus benefiting for drug delivery.

The invention discloses a fluorescence immunochromatographic assay and kit for quantitatively detecting cardiac troponin T (cTnT). The fluorescence immunochromatographic assay for quantitatively detecting cTnT realizes fluorescence quantitative detection on the basis of optimizing various constituent parts of test paper by using excellent fluorescence characteristic of quantum dots and combining a bicolor marking technology and an immunochromatographic technology. Compared with the conventional colloidal gold immunochromatographic assay, the fluorescence immunochromatographic assay disclosed by the invention has the advantages of good marking stability, low non-specificity, high sensitivity, wide linear range and quantifying accuracy. The fluorescence immunochromatographic kit disclosed by the invention is used for carrying out quantitative detection on the cTnT and detecting whole blood, blood serum and blood plasma samples and is suitable for different levels of hospitals and particularly good for wide popularization in primary hospitals and clinics.

The invention discloses an antihuman-globulin blood matching detection card with different formulas at the primary side and the secondary side. The antihuman-globulin blood matching detection card comprises 6 micro-column pipes or 8 micro-column pipes for labeling the primary side and the secondary side, wherein the 6 micro-column pipes are pipes 1-6 in sequence from left to right, i.e., the pipe 1, the pipe 3 and the pipe 5 are the primary-side pipes, and the pipe 2, the pipe 4 and the pipe 6 are secondary-side pipes; the primary-side pipes are used for detecting irregular antibodies and complement component C3d; the secondary-side pipes are used for detecting the irregular antibodies; the primary-side pipes contain anti-IgG, anti-C3d and 0.1%-0.5% of NaCl; and the secondary-side pipes contain anti-IgG and 0.2-0.6% of NaCl. The antihuman-globulin blood matching detection card disclosed by the invention has the advantages that the primary-side pipes and the secondary-side pipes adopt different formulas, so that the specificity and the sensitivity of blood-matching detection are improved and the problems of non-specificity, false positive and false negative generated in blood matching of the primary side and the secondary side in the existing antihuman-globulin detection technology are solved.

The present invention is internal standard gene suitable for quantitative and qualitative PCR detection of foreign gene of transgenic cotton, tomato, rice and rape and copy number analysis and its application. Based on the intraspecific non-specificity, interspecific specificity, monocopy or low copy number, and through bioinformatical analysis and biological experiment, four internal standard genes, including cotton SADI, tomato LAT52, rice SPS and rape HMGI/Y gene are found out separately in cotton, tomato, rice and rape. The detection sensitivity and precision of these internal standard genes are experiment proved in PCR method. At the same time, the quantitative and qualitative PCR detection systems for foreign gene of transgenic cotton, tomato, rice and rape are established separately based on these internal standard genes, and corresponding PCR detection and analysis platforms are established.

The invention discloses an oriented-polymerization fluorescent probe PCR which is characterized in that conventional primer and probe are specifically combined while respective oriented polymerizationis added. A reverse base sequence at the 5' end of the opposite-side former primer is added to the front of a primer pair along the 5'-3' direction to form a '5' reverse complementary sequence' chimeric primer pair for oriented polymerization; through the 5' end complementation, the 3' tails of the amplification products serve as a template of each other and form mutual primer amplification; based on PCR hypersensitivity, the sensitivity 3-5Ct is further improved while the non-specificity is reduced, or the amplification of 5 cycles can be reduced at the same sensitivity; the 3' lengthened tail end of the fluorescent probe and the middle sequence of the probe are reversely complementary and form self-hybridization in pairs, then the quencher approaches the fluorophore group through mutualpolymerization at the tail part and the middle part so as to reduce the base line fluorescence and probe non-specificity; once a long-sequence target molecular chain is specifically hybridized with the primer and the probe chain, the pairwise polymer pair and probe with low relative competitive ability are molten and specifically combined with target molecule for amplification, and the probe is hydrolyzed by polymerase to generate fluorescence in direct proportion to the target molecule.

The invention relates to a diarrhea-related pathogen detection kit combining multiple RT-PCR with a gene chip. According to the kit, a multiple-specificity conserved degenerate primer composition and a probe composition are adopted to detect one or more of 26 diarrhea-related pathogens, and at the same time, endogenous control, positive control and negative control are arranged. By the adoption of the diarrhea-related pathogen detection kit combining the multiple RT-PCR with the gene chip, the coverage rate of target sequences of high-mutation pathogens is increased, the problem of non-specificity cross reaction between multiple primers and probes is avoided, the purpose can be achieved that a single reaction system synchronously detects more than 20 kinds of diarrhea-related pathogens, and a detection tool which is simple, sensitive, rapid, large in throughput and capable of multi-index parallel detection is provided for diarrhea-related pathogen detection.

The invention relates to the field of biology and especially relates to an ATP7B (ATPase Cu2+transporting beta polypeptide) gene mutation detection primer set and kit, an ATP7B gene mutation detection method and uses of the ATP7B gene mutation detection primer kit. The primer set relates to the most area of the ATP7B gene, comprises an exon and an intron, acquires complete ATP7B gene nucleotide sequence cases and determines a mutation site. The primer set aims at long fragment amplification, a single stripe can be obtained through amplification under appropriate conditions, and a nonspecific stripe is not produced. The amplified fragment is long so that compared with the short fragment amplification method, the long fragment amplification method produces a uniform sequencing result and data analysis is simpler.

The invention belongs to the field of aquatic product fermented feeds and particularly discloses an efficient feed capable of promoting the growth rate and non-specificity immunity of penaeus vannamei boone. The efficient feed is characterized by being prepared from the following components in parts by weight: malt powder, spiral seaweed powder, fermented soybean meal, clam powder, probiotics freeze-dried powder, xylanase, cellulase, glucoamylase, beta-amylase, loofah sponge, active yeast powder, activated zeolite modified low-glue kelp powder and the like. The columnar compact fermented pellet feed which has a spiral groove on the surface is prepared by fermenting main materials including the malt powder and the like with nutrient substances; the main materials are matched with the nutrient substances so that nutrients are comprehensive; the fermentation is carried out by two phases; in the second phase, the fermentation time is prolonged by an added compound enzyme and a slow-release yeast compound block mass; fermented nutritional ingredients are enriched and the growth of the penaeus vannamei boone is promoted; and compound enzyme residual components can further improve the non-specificity immunity of the penaeus vannamei boone and the efficient cultivation of the penaeus vannamei boone can be commonly promoted.

A contrast agent based on graphene oxide material and preparation method therefor. The contrast agent has a structure represented by formula I: A-(B-R)n, wherein A is graphene oxide, R is gadolinium metal complex, B is bridging molecule, and A, B and R are bonded to each other via chemical bond. The contrast agent has characteristics of high relaxation, good stability, non-specificity and the like.

The invention relates to the technical field of livestock vaccine, and in particular relates to a method for detecting a hemagglutination inhibition antibody of chicken infectious bronchitis. The method comprises the following steps: treating serum to be detected, namely, by taking kaoline suspension as a serum treatment liquid for treating serum to be detected, adding the supernate into chicken erythrocyte blood corpuscle mud so as to obtain serum which is diluted in a ratio of 1:4 and is to be detected; performing hemagglutination inhibition test, namely, adding 25mu l of the serum which is diluted in a ratio of 1:4 and is to be detected into 25mu l of antigen with 4HA units, and performing hemagglutination judgment. By adopting the negative serum treated by using a method for removing non-specific reaction of the serum, the infectious bronchitis hemagglutination inhibition evaluation titer is less than 1:4, and by adopting the infectious bronchitis positive serum treated by using the method, the infectious bronchitis hemagglutination inhibition evaluation titer is reduced by 0.5-1 titer, the non-specificity reaction is removed, and the accuracy of the infectious bronchitis hemagglutination inhibition experiment result is improved.