Method for detecting hemagglutination inhibition antibody of chicken infectious bronchitis

A technology of hemagglutination inhibition and bronchitis, which is applied in the field of chicken infectious bronchitis hemagglutination inhibition antibody detection, can solve the problems of difficult non-specific reactions and poor processing results, and achieve the effect of improving accuracy

Inactive Publication Date: 2015-06-24
YEBIO BIOENG OF QINGDAO
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Problems solved by technology

[0003] At present, there are many methods for removing non-specific reactions in serum studied at home and abroad, such as adding chicken red blood cells, heating at 56°C, and adding polypropylene glycol, etc., but the results of these methods are not good.
In addition, the non-specific reaction of serum in infectious bronchitis hemagglutination inhibition test is relatively high, and it is relatively more difficult to remove its non-specific reaction

Method used

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  • Method for detecting hemagglutination inhibition antibody of chicken infectious bronchitis
  • Method for detecting hemagglutination inhibition antibody of chicken infectious bronchitis

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Embodiment 1

[0023] 1. Antigen preparation

[0024] 1. Dilute the respiratory type IBV-M41 strain, glandular stomach type IBV-YBX strain and kidney type IBV-T strain 100 times with sterile saline respectively, and inoculate 10-day-old SPF chicken embryos into the allantoic cavity. 0.1ml, incubate in a chicken embryo incubator at 37°C, illuminate the embryos once every 24 hours, and discard the dead embryos. Up to 48 hours, regardless of whether they died or not, they were all taken out and cooled in a refrigerator at 4°C. According to this method, 3 batches of each antigen were prepared.

[0025] Among them, respiratory type IBV-M41 strain and renal type IBV-T strain virus seeds were purchased from China Veterinary Drug Control Institute. The glandular stomach type IBV-YBX strain was collected by the applicant himself.

[0026] 2. Collect the allantoic fluid, put it into a 500ml centrifuge bottle, centrifuge at 3000rpm for 30 minutes, and take the supernatant.

[0027] 3. Put the super...

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Abstract

The invention relates to the technical field of livestock vaccine, and in particular relates to a method for detecting a hemagglutination inhibition antibody of chicken infectious bronchitis. The method comprises the following steps: treating serum to be detected, namely, by taking kaoline suspension as a serum treatment liquid for treating serum to be detected, adding the supernate into chicken erythrocyte blood corpuscle mud so as to obtain serum which is diluted in a ratio of 1:4 and is to be detected; performing hemagglutination inhibition test, namely, adding 25mu l of the serum which is diluted in a ratio of 1:4 and is to be detected into 25mu l of antigen with 4HA units, and performing hemagglutination judgment. By adopting the negative serum treated by using a method for removing non-specific reaction of the serum, the infectious bronchitis hemagglutination inhibition evaluation titer is less than 1:4, and by adopting the infectious bronchitis positive serum treated by using the method, the infectious bronchitis hemagglutination inhibition evaluation titer is reduced by 0.5-1 titer, the non-specificity reaction is removed, and the accuracy of the infectious bronchitis hemagglutination inhibition experiment result is improved.

Description

technical field [0001] The invention belongs to the technical field of poultry vaccines, and in particular relates to a detection method for chicken infectious bronchitis hemagglutination inhibitory antibodies. Background technique [0002] Chicken infectious bronchitis (IB) is an acute and highly contagious respiratory disease caused by infectious bronchitis virus (IBV), characterized by tracheal rales, coughing, sneezing; runny nose in young chickens, egg production of laying hens It is characterized by a reduction in egg quality and decreased egg quality; sick chickens often die due to respiratory, kidney or digestive tract infections. Inherited antigen characteristics, the virus itself has hemagglutination, and it must be specially treated to release the receptors for agglutinating red blood cells, and the hemagglutination appears. At present, the efficacy test of infectious bronchial vaccine mainly adopts the method of challenge and protection. There is a risk of deto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 宋新宇王红窦小龙李陆梅张恒邹敏史淑艳程增青
Owner YEBIO BIOENG OF QINGDAO
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