The present invention belongs to the technical field of
molecular biology, and particularly relates to a whole-
genome chip for
germplasm traceability of yellow-feathered broilers and its application. It is prepared from probes composed of 9,737
germplasm traceability sites of yellow-feathered broilers and 261
disease resistance-related sites, with a total of 9,998 SNP molecular markers. The physical position information on the chicken
reference genome is shown in Table 1. The
chip sites cover all chromosomes, are evenly distributed on each
chromosome, with an average distance of 95 Kb, having the
advantage of low marker density. The cost of the liquid-phase
chip directly depends on the marker density, enabling genomic breeding related to chicken
disease resistance at a relatively low cost. Moreover, the chip product has good stability, with a
genotype consistency rate of >99% for repeated samples, and can reliably achieve
germplasm traceability for local chicken breeds, commercial chicken breeds, and wild red
jungle fowl populations at home and abroad, as well as commercial broilers of yellow-feathered broilers with highly similar
population genetic backgrounds.