This invention belongs to the field of animal
cell culture technology, specifically relating to chicken
embryo fibroblast cell lines, their culture media,
acclimatization methods, and applications. This application provides a chicken
embryo fibroblast cell line derived from… Gallus gallus The chicken
embryo fibroblast suspension cell line CEF-P17 3D is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: C202661 and deposit date of March 31, 2026. This application also provides a culture medium for culturing the chicken embryo
fibroblast cell line and a method for acclimatizing the chicken embryo
fibroblast cell line, which shortens the
acclimatization period, simplifies the operation, and is suitable for large-scale culture. This application also provides the application of the culture medium for the chicken embryo
fibroblast cell line. Furthermore, this application also provides the application of the chicken embryo fibroblast cell line in the preparation of
cultured meat.