213 results about "Gallus gallus gallus" patented technology

The invention relates to a method for ecologically breeding black-bone chickens in a medlar garden, comprising the following steps: 1. medlar garden construction: the growth of garden nursery stocks takes more than one year, strong stocks have no pest infection, row spacings of planted plants are 1 m*2 m, 333 plants are planted in per mu, forage grass or/and vegetables, i.e. lucernes, small rapes, small Chinese cabbage, and the like are interplanted among rows of medlar plants, a net-shaped iron-wire protective guard with the height of 1.5 m is constructed around the medlar garden, and movable water tanks and forage tanks are placed in the medlar garden; 2. simple chicken house construction: a finished chicken house is in a steel bar pipe frame and asbestos tile structure, a roof structure is in a monoclinic way, the height of circumferential asbestos tiles is 1.5 meters, the upper part of the chicken house is provided with a steel plate net, and the chicken house is internally provided with the forage tanks and drinking bowls in a matching way; 3. brooding, grazing and manual forage supplement: chicks are collected to be brooded for 4-5 weeks and then bred in the medlar garden for 8-10 weeks without original temperature, and two batches of the black-bone chickens are grazed and bred every year; 4. breeding density: the number of each batch of the black-bone chickens which are averagely bred on the earth per mu is properly controlled between 100 and 150; and 5. a breeding way: an all-in and all-out breeding way is carried out for the same batch of the black-bone chickens.

The invention discloses a method for breeding blue-lower-leg spotted-brown-feather laying hens with high egg yields. The method comprises the following steps of: artificially inseminating and mating a large quantity of Huainan spotted-brown cocks serving as male parents and Loman laying hens serving as female parents, collecting hatching eggs and hatching an F1 generation; identifying cocks and hens according to the colors of feathers and lower legs, eliminating white-feather yellow-lower-leg chicken which are cocks, reserving spotted-brown-feather blue-lower-leg chicken which are hens for serving as breeding hens, and performing back crossing by taking Huainan spotted-brown cocks as male parents to produce an F2 spotted-brown-feather generation; crossly mating and fixing the F2 generation and performing family breeding to produce an F3 generation; and performing gradual pure breeding on the F3 generation and later generations and obtaining the blue-lower-leg spotted-brown-feather laying hens with high egg yields according to egg yield, protein, lower leg color, feather color and bodily form, wherein the feathers are spotted-brown feathers; and the bodily form refers to delicate and pretty bodily form, blue lower leg and blue beak. According to the method, an appropriate high-egg-yield gene is introduced into a local chicken breed, and the proteins and macroscopic features of the local chicken breed are kept simultaneously.

The first breeding process of black-shin yellow hybrid chicken adopts pure-blood black-shin yellow chicken or synthetic black-shin yellow chicken as male parent and pure or synthetic three-yellow chicken or recessive white-feather chicken as female parent in producing the hybrid chicken. The second breeding process adopts pure-blood black-shin yellow chicken or synthetic black-shin yellow chicken as male parent and the F1 generation hybrid produced in the first process as female parent in producing the F2 generation hybrid chicken. The hybrid chicken has the outer appearance of black-shin yellow chicken as well as powerful disease resistance, fast growth speed, high egg yield, high feed converting rate and other heterosis.

The invention belongs to the technical field of chicken molecule mark preparation, and in particular relates to and builds a method for identifying molecules of recessive white feather genes of chicken. In the method for identifying the molecules of the recessive white feather genes of the chicken, nucleotide sequences of recessive white feather control genes of the chicken (TYR genes inserted with retroviruses) and colored feather control genes TYR genes are utilized to design a primer pair 1 and a primer pair 2 respectively, wherein the primer pair 1 is used for detecting recessive white feather allelic genes; and the primer pair 2 is used for detecting colored feather allelic genes. The method comprises the following steps: extracting genomic DNA of the chicken to be identified and performing PCR amplification by using the primer pairs 1 and 2; performing electrophoresis detection on amplification products; judging the type of the feather color genes of the chicken according to electrophoresis results; if the amplification products are produced by only the primer pair 1, determining the feather color genes are recessive white feather homozygote (the phenotype is a white feather); if the amplification products are produced by only the primer pair 2, determining the feather color genes are non-recessive white feather homozygote (the phenotype is either a colored feather or the white feather); and if the amplification products are produced by both of the primer pairs 1 and 2, determining the feather color genes are recessive white feather heterozygote (the phenotype is either a colored feather or the white feather). The invention provides the quick and convenient method for identifying the molecules of the recessive white feather genes of the chicken.

The invention discloses a method for culturing a high-quality green-shank pockmarked-feather poulard. The method comprises the following steps: (1) mating a green-shank pockmarked-feather cock with a yellow-shank pockmarked-feather hen which grows fast to produce an F1 generation; (2) back-crossing the green-plank pockmarked-feather hen of the F1 generation with the green-shank pockmarked-feather cock of the parental generation to produce an F2 generation; (3) selecting a cock and a hen which grow fast from the green-shank pockmarked-feather chickens of the F2 generation for crossing to obtain an F3 generation; and (4) selecting a cock and a hen which grow fast from green-shank pockmarked-feather chickens of the F3 generation for self-reproduction and flock enlargement to obtain an F4 generation, i.e. the high-quality green-shank pockmarked-feather poulard of the new species. The high-quality green-shank pockmarked-feather poulard cultivated by the method has the advantages of beautiful appearance, specific figure, favorable meat quality, delicious meat taste, strong disease resistance and fast growth, and is applicable to the commercial production.

The invention provides a method for producing breed of a black-skin dorking, which comprises the following steps: (1) building a line taking a Tianfu black-bone chicken with black feathers and black skin as a maternal line, adopting a family method of breeding to perform pure line breeding to obtain a Tianfu balck-bone chicken with black skin; (2) conducting hybridization and backcross by taking a white plymouth rock as a male parent and taking the Tianfu black-bone chicken with black skin a female parent to obtain a group containing more than 75% of Tianfu black-bone chicken (B1 line); (3) conducting hybridization and backcross by taking the Tianfu black-bone chicken with black skin as a male parent and taking a Sichuan mountain black-bone chicken with back feathers and black skin as a female parent and introducing 25% of Sichuan mountain black-bone chicken into the Tianfu black-bone chickens (B2 line); and (4) performing hybridization by taking the B1 line as a male parent and taking the B2 line as a female parent, carrying out crossing fixation, closing and breeding, and adopting the family method of breeding and a positive and negative recurrent selection method to perform the pure line breeding to obtain a new black-skin dorking line. The new line has the advantages of unified appearance, black (dark) carcass skin, high production performance and good mouthfeel, flavor and nourishing effect.

The invention discloses a primer composition for discriminating a green-shelled-egg chicken genotype and a use thereof. The primer composition comprises two primer pairs, one primer pair comprises SLCO1B3 gene inserted EAV-HP sequence primers respectively shown in the formulas of SEQ ID NO. 1 and 2, and the other primer pair comprises SLCO1B3 gene non-inserted sequence primers respectively shown in the formulas of SEQ ID NO. 1 and 3. A DNA genome of a chicken to be detected is used as a template and is subjected to PCR amplification under the action of the primer pairs so that a PCR amplification product is obtained. Through detection of the amplified 209bp and 312bp bands, it is determined if the chicken to be detected can produce green-shelled eggs. The primer composition can discriminate the green-shelled-egg chicken genotype.

The present invention relates to an excellent-quality chicken maternal strain, in particular to a method for the strain breeding of the dark-shank spotty plumage chicken with dwarf gene, which belongs to the technical field of the dark-shank spotty plumage chicken with dwarf gene. The breeding procedures of the present invention are (1) selecting a cock of Chongren chicken and a hen having the dark-shank partridge in the grandparent hen group of the dwarf yellow-feather chicken as the breeding materials and selecting a normal-shaped cock having the dark-shank partridge character in the F1 generation after the hybridization of the cock and the hen; (2) Selecting the dark-shank partridge normal-shaped cock to be mated with the hen of the dark-shank partridge dwarf yellow-feather chicken and selecting the dark-shank partridge dwarf cock and the dark-shank partridge dwarf hen in the F2 generation; (3) cross-breeding and locking the selected dark-shank partridge dwarf cock and the dark-shank partridge dwarf hen so as to get the present invention after the breeding from generation to generation. Since the body of the maternal-strain chicken is short cultivated through the present invention, the chicken has the outstanding advantages of precocious puberty, high-yield of eggs and less feed consumption and cultivated space saving, which greatly reduces the production cost of the commodity generation chicken and improves the comprehensive benefit of the cultivation.

A method for culturing the new variety of reed-catkins chicken includes such steps as choosing mutant cock and hen from local reed-catkins chicken, self-reproducing or transverse crossing, Choosing the cock and hen with transverse spots and light reed-catkins color, lateral crossing, choosing homozygotes, selective culturing, and repeating the previous two steps until stable heredity.

The breeding process of high-yield variety with green (black or yellow) shank and recessive white feather includes the following steps: hybridization between recessive white feather chicken or green (black or yellow) shank and recessive white feather chicken and high-yield brown-shell egg or white-shell egg chicken to obtain F1 generation hybrid individuals; selfing reproduction of F2 generation with the F1 generation and selecting green (black or yellow) shank and white feather chicken W; test crossing green (black or yellow) shank yellow chicken with W, selecting green (black or yellow) shank and pure white feather hem and cock and self-reproduction to produce Y; repeated test cross and selection to obtain recessive white feather chicken individuals; and self-reproduction to expand colony, constitute genealogy and obtain new chicken variety of high-yield variety with green (black or yellow) shank and recessive white feather.

The invention relates to a method for producing chicken flavor collagen concentrated soup by utilizing chicken skeletons and chicken claws. The method comprises the following steps of: adding spices of chives, ginger, and the like to chicken skeletons and chicken claws; steaming, boiling and extracting under high pressure at low temperature; separating; concentrating at low temperature in vacuum; blending; homogenizing under high pressure; and encapsulating to obtain a chicken flavor collagen concentrated soup product. The product produced by adopting the method has the advantages of low cost and easy obtaining of raw materials, is convenient for industrial production and can be applied to scaled production. In the processing process of the product, the processes of extracting under high pressure at low temperature, concentrating at low temperature in vacuum, and the like, are adopted, which better stores the fragrance and the flavor of the product, avoids the loss of the fragrance and the flavor and also avoids the breakage of collagen; and because a large quantity of collagen contained in the chicken claws is sufficiently extracted, the collagen content of the product is high. Moreover, the collagen is a major constituent in the skin and occupies more than 71 percent of the protein in skin cells, and the collagen can ensure that the cells become plump so as to ensure that the skin can be engorged and keep the elasticity and the moisture, thus the collagen has important effect.

The invention discloses a selective breeding method of dark-shank partridge chickens. The method comprises the following steps: (1) selecting hens and cocks of 105 days or older from Guangxi Nandan-yao chickens to establish a family, cultivating a first male parental N line, and selecting cocks having characters meeting selection requirements as a first male parent; (2) selecting cocks and hens from Liuzhou partridge chickens to establish a family, cultivating a first female parental L line, and selecting hens having characters meeting selection requirements as a first female parent; (3) selecting cocks from Jiangxi Chongren partridge chickens and hens from Shaobo chickens, performing hybridization to obtain male parental hens, then performing hybridization on the mal parental hens and thecocks from the Nandan-yao chickens to obtain a dark-shank partridge chicken line group, establishing a family cultivation terminal male parent Q line, and selecting cocks having characters meeting selection requirements as a parent generation male parent; (4) performing mating on the first female parent L line hens and the first male parent N line cocks to obtain a parent generation NL line group, and selecting parent generation female parent hens according to requirements; and (5) performing mating on the parent generation cocks and hens to obtain commercial generation chicks namely a new line of dark-shank partridge chickens.

The invention discloses a cultivation method of black-feather pink-egg layer chickens. The cultivation method comprises the following steps of: (1) selecting a brown-egg layer chicken great-grandparent A series cock, a brown-egg layer chicken great-grandparent B series cock, a brown-egg layer chicken grandparent A series cock, a brown-egg layer chicken grandparent B series cock or a brown-egg layer chicken parent AB series cock as male parents and extracting seminal fluid by using a manual mode; (2) selecting a highest ranking imperial concubine hen as a female parent to carry out artificial fertilization; (3) collecting hatching eggs laid by the highest ranking imperial concubine hen in two days after the fertilization and hatching the eggs; and (4) obtaining hatched hens namely the black-feather pink-egg layer chickens. The body shape, the weight and the appearance of the cultivated black-feather pink-egg layer chicken are similar to that of a native chicken, the color of the feathers of the cultivated layer chicken is black, and laid eggs have pink shells. The egg laying property of the cultivated layer chicken is good, the annual egg yield can reach 210-230, and the later egg weight is about 50g. The black-feather pink-egg layer chickens can be raised in cages or stocking, have high spent chicken price and are suitable for large-scale cultivation.

The invention relates to a breeding method of high-yield new-breed of silver-feather chickens laying green-shell eggs. The method includes the following steps that firstly, a hen laying eggs with green shells and a parent-generation female parent cock of a chicken, hatched in an automatic sexing mode, having gold and silver feathers and laying high-yield eggs with red shells are selected from local chicken breeds and are subjected to hybridization to obtain F1 generations; secondly, the F1 generations are subjected to crossing, purely-white-feather cocks and purely-white-feather hens laying eggs with green shells are selected from F2 generations, and self-breeding and breed enlarging are carried out; thirdly, test mating and identification are carried out on hens having purely-white feathers and laying eggs with green shells and cocks with purely-white feathers in later self-breeding generations by a cock having yellow-red feathers and laying eggs with brown shells and a hen having yellow-red feathers and laying eggs with brown shells respectively, individuals of which cock and hen green shell and silver feature genes are homozygotes are selected out, and self-breeding and breed enlarging are carried out; fourthly, the third step is repeated until stable inheritance of sliver feather and green shell characters is achieved; fifthly, the fourth step is repeated until a high-yield new breed of chickens having silver feathers and laying eggs with green shells is obtained. By means of the method, the egg yield can be substantially increased, two-way crossing can be carried out between the high-yield new breed as a female parent and other breeds, and the automatic sexing rate, green shell rate and the like of later generations can reach 100%.

The invention belongs to the field of molecular genetics and particularly relates to a molecular marking method of a mutant site related to a 5' control region of a chicken miR-1b-3p gene and chicken sexual maturity and laying number traits and an application thereof in chicken breeding. The mutant site is a -734(G is greater than A) site. Genetic typing among three different groups is performed and the laying trait associated analysis is performed by means of a KASP technology, and the result shows that the site is extremely related to the laying start age and egg numbers in 32 weeks and 48 weeks (p is smaller than 0.01), and the laying start ages of genes AA and AG are extremely obviously earlier than that of GG gene, the egg numbers of the genes AA and AG in 32 weeks is extremely obviously greater than that of the gene GG but the egg number of the gene AA in 48 weeks is extremely obviously greater than those of the genes AG and GG. By detecting the molecular marker related to the laying trait, egg varieties/strains which are early to mature and high in yield are bred favorably, and favorable help is brought to local chicken breeding work.

The invention discloses a recessive white feather Beijing fatty chicken breeding method. The recessive white feather Beijing fatty chicken breeding method comprises the following steps: firstly, hybridizing purebred Beijing fatty female chickens with recessive white feather male chickens to obtain an F1 generation, secondly, crossing the F1 generation to obtain an F2 generation, thirdly, selecting fine quality white feather female chickens from the F2 generation to backcross the fine quality white feather female chickens with the purebred Beijing fatty male chickens to obtain an F3 generation, fourthly, crossing the F3 generation lou to obtain an F4 generation lou, fifthly, selecting a white feather crested F4 sub lou from the F4 generation lou and conducting crossing to obtain an F5 generation crested white feather Beijing fatty chicken sub lou, meanwhile, selecting a white feather bearded F'4 sub lou from the F4 generation lou and conducting crossing to obtain an F'5 generation bearded white feather Beijing fatty chicken sub lou, sixthly, conducting reciprocal hybridization on the F5 generation sub lou with the F'5 generation sub lou to obtain an F6 generation lou, seventhly, picking white feather chickens with all crested, breaded, feather-legged and five-toed features, persevering breeds of the picked chickens and conducting crossing to obtain an F7 generation, eighthly, close-breeding the F7 generation to obtain recessive white feather Beijing fatty chickens. The recessive white feather Beijing fatty chickens are high in growth speed and good in meat quality.

A method for culturing the high-quality meat chicken with green (or yellow) shin and dominant white feather includes hybridizing between local meat chicken and fast-growing white-feather meat chicken to obtain generation F1, selfing to obtain generation F2, choosing the green cyellow-spine white-feather chicken, test crossing with color-feather chicken, choosing deominant white-feather chicken, reproduction, enlarging clony, and crossing with moderm layer.

The invention provides unconventional protein compound chicken feed special for young broilers, belonging to the feed special for particular animals. The feed comprises the following components according to the parts by weight: 0.1-0.2 part of enzyme preparation, 30-40 parts of corns, 30-38 parts of wheat, 3-5 parts of peanut meal, 1-5 parts of albumen powder, 0-2 parts of hydrolyzed feather meal, 2-4 parts of cottonseed meal, 0-3 parts of waste starch size, 0-4 parts of alcohol lees, 0-3 parts of cottonseed protein, 1-3 parts of rapeseed meal, 0-3 parts of germ meal, 1.8-2.0 parts of fat, 1.48-1.56 parts of stone meal, 1.0-1.3 parts of dicalcium phosphate, 0.3-0.32 part of salt, 1.7-2.0 parts of lysine, 0.12-0.15 part of threonine, 1.3-1.8 parts of methionine, 0.1 part of choline chloride and 0.5 part of premix. The feed solves the problem of shortage of conventional protein chicken feedstuff resources and uses the industrial wastes as raw materials formulas, thus saving the energy and protecting the environment. The feed is suitable for the daily ration of the 0-21-day old broilers.

The invention relates to a breeding method of red Yao ma chickens. The breeding method is characterized by comprising the following steps of step 1, selecting parent breeding chickens; step 2, establishing a pure line and a family for base group breeding chickens; step 3, identifying the pure line of base group breeding chickens and the family of base group breeding chickens; step 4, establishing a core group and a family for the selected and remaining breeding chickens and producing the new generation of family; step 5, producing ancestral breeding chickens through the new generation of family of breeding chickens in the step 4, producing parent breeding chickens through the ancestral breeding chickens, producing goods generation of chickens through the parent breeding chickens and obtaining the new strain of red Yao ma chickens. According to the breeding method of the red Yao ma chickens, the feather colors of the bred red Yao ma chickens are basically as same as each other, the feather colors of the appearance of cocks are red, black and yellow and the purity is 100%, and the ma feather colors of the appearance of hens are dark brown and red and the purity is more than 95%; the egg laying number of 66 week age of hen breeding is more than 140 every day and the hatching egg fertilization rate is more than 90%; 120 day age of cocks are more than 2100 g, 120 day age of hens are more than 1600 g, and the weight coefficient of variation is less than 10%.

The invention relates to a breeding method of black Yao ma chickens. The breeding method is characterized by comprising the following steps of step 1, selecting parent breeding chickens; step 2, establishing a pure line and a family for base group breeding chickens; step 3, identifying the pure line of base group breeding chickens and the family of base group breeding chickens; step 4, establishing a core group and a family for the selected and remaining breeding chickens and producing the new generation of family; step 5, producing ancestral breeding chickens through the new generation of family of breeding chickens in the step 4, producing parent breeding chickens through the ancestral breeding chickens, producing goods generation of chickens through the parent breeding chickens and obtaining the new strain of black Yao ma chickens. According to the breeding method of the black Yao ma chickens, the feather colors of the bred black Yao ma chickens are basically as same as each other, the feather colors of the appearance of cocks are red, black and yellow and the purity is 100%, and the ma feather colors of the appearance of hens are dark brown and red and the purity is more than 95%; the egg laying number of 66 week age of hen breeding is more than 140 every day and the hatching egg fertilization rate is more than 90%; 120 day age of cocks are more than 2100 g, 120 day age of hens are more than 1600 g, and the weight coefficient of variation is less than 10%.

The invention discloses a kit used for detecting body size traits of chicken and a molecular breeding method of the chicken and belongs to the field of molecular genetics. MassARRAY system matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and a single-primer extension reaction are combined, miRNA (micro ribonucleic acid)-1658 (rs16681031 C/G) single-SNP (single nucleotide polymorphism) site genotyping detection on F2 generation resource population samples of Gushi chicken and Anak chicken is performed, and correlation analysis with the size traits of F2 generation resource populations is performed. Results show that the chicken miRNA-1658 gene SNP (single nucleotide polymorphism) is obviously correlated with the body size traits of the chicken; a GG gene facilitates obvious increasing of chest breadth, crista breadth and body slanting length of a four-week-year-old chick; chicken flocks with excellent genetic resources can be quickly built by taking the GG gene as assistant selection of the body size trait of the four-week-year-old chick and as a molecular genetic breeding marker of the four-week-year-old chick.

A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.

The invention discloses an ecological breeding method for chickens. The method comprises the following steps of (1) culture environment selection: a chicken house is built in a forest in a mountainous area, and the chickens are bred in the forest in a free-ranging way; (2) seed selection and egg laying: high-quality breeds with the small body size after the mature are selected; (3) hatching: hatching eggs are hatched for 21 days in a hatching machine with the temperature being 37 to 37.8 DEG C and the relative humidity being 50 percent to 60 percent; (4) chickling management: chicklings are fed for 5 to 7 days in a chickling room with the temperature being 28 to 30 DEG C to obtain heating-free chicklings; (5) heating-free chickling management: 35-38-day-old heating-free chicklings are subjected to artificial immunization; (6) feed feeding: artificially prepared compound feed is used as major feed, and natural ingestion supplementation is matched; (7) daily management: an ultraviolet lamp is arranged outside the chicken house, a music player is hung outside the chicken house, and the music is played for 3 to 4 hours respectively in the morning and in the afternoon. The ecological breeding method has the advantages that the adaptability of the chickens is enhanced through the music playing, the health growth of the chickens is promoted, meanwhile, the production cost is reduced, the protein content of the chickens is increased, and the egg quality is improved.

The invention relates to a method of utilizing yolk antibody instead of serum antibody to evaluate infection state of breeder flock. The method of the invention comprises the following steps: collecting chicken serum and eggs, respectively; respectively detecting antibody levels of serum and yolk by REV, REOV, ALV-J, ALV-AB, CAV and IBDV enzyme linked immunosorbent assay (ELISA) kits from IDEXX company; comparatively analyzing serum and yolk antibody OD value correlation coefficient by SPSS Statistics 17.0 to obtain correlation coefficient of an optimum dilution factor of yolk antibody and a serum antibody OD value according to ELISA detection. The detecting result further indicates that the positive and negative result consistent rate of yolk sample and the serum sample at the optimum yolk dilution factor is 96%-100%. The result of the invention indicates that the serum samples can be replaced by yolk when various infection states of breeder flock are detected by ELISA kits provided by IDEXX for detection with serum samples, so that the infection state of breeder flock can be detected by detecting yolk antibody level.