15 results about "Gallus gallus gallus" patented technology

The invention discloses a structural variation marker based on an ARL13B gene and applied to Wenchang chicken breeding and a detection method of the structural variation marker, and relates to agricultural bioengineering, the sequence of the structural variation molecular marker is SEQ ID No: 01, and the structural variation molecular marker is located on a Wenchang chicken gene reference genome ASM4043665v1, Gallus gallus chick; the molecular marker is characterized in that the position of Genbank: GCA040436655.1 is CM080655.1: 108497195-108497244, and the molecular marker with the structural variation is a deletion site; at the same week age, the weight of an individual with the deletion structure variation marker sequence is obviously higher than that of a non-deletion individual, and the molecular marker obtained by the invention can be applied to Wenchang chicken breeding.

The invention relates to the technical field of mastopathy medicines, and discloses a traditional Chinese medicine patch for treating breast nodules and a preparation process thereof. The traditional Chinese medicine patch comprises the following raw materials: safflower, pollen typhae, caulis spatholobi, angelica sinensis, trogopterus dung, lumbricus, gastrodia elata, pseudo-ginseng, cassia twig, thunberg fritillary bulb, frankincense, endothelium corneum gigeriae galli, rhizoma atractylodis, platycodon grandiflorum, curcuma zedoary, radix semiaquilegiae, radix bupleuri and salvia miltiorrhiza. Through reasonable compatibility of the raw materials and effective extraction of the active ingredients of the traditional Chinese medicines, the effects of the raw materials can be maximized, all the components have a synergistic effect, the permeability is high, the efficacy is lasting, and the traditional Chinese medicine preparation has the effects of soothing liver and regulating qi, promoting blood circulation to remove meridian obstruction, eliminating stasis and dissipating binds, clearing heat and dispelling wind, and nourishing yin and relieving pain; the traditional Chinese medicine composition has obvious curative effects on various breast diseases such as breast nodules, hyperplasia of mammary glands, pains, lumps and the like, the nodules disappear, and the mammary glands basically have no pains and uncomfortable symptoms.

The invention belongs to the technical field of medicine, and discloses a traditional Chinese medicine composition for treating chronic rhinitis and a preparation method thereof.The composition is prepared from, by weight, 10-20 parts of astragalus membranaceus, 10-20 parts of radix saposhnikoviae, 10-20 parts of rhizoma atractylodis macrocephalae, 5-10 parts of schizonepeta, 5-10 parts of rhizoma acori graminei, 5-10 parts of cicada slough, 5-10 parts of endothelium corneum gigeriae galli and 5-10 parts of flos magnoliae. The preparation method of the composition comprises the following steps: S1, cleaning the traditional Chinese medicines with water, and soaking for 0.5-1 hour; s2, decocting the traditional Chinese medicinal materials treated in the step S1 with big fire for 20 minutes, then turning to soft fire, and stewing out medicine juice; s3, pouring out the liquid medicine obtained in the step S2, adding water again to submerge the medicinal materials, boiling with big fire, turning to soft fire, and decocting for 15 minutes; s4, mixing the liquid medicine obtained in the step S2 and the liquid medicine obtained in the step S3 to obtain the traditional Chinese medicine composition for treating rhinitis. The composition has the effects of invigorating the spleen, benefiting the lung, tonifying qi and opening orifices, and is stable in curative effect and short in treatment cycle.

The present invention relates to a method for preparing a fermented extract of Gallus gallus var. domesticus, which, through fermentation and enzyme treatment of Gallus gallus var. domesticus, is convenient to consume , has an increased nutrient content, is easily absorbed, and has excellent palatability. The method for preparing a fermented extract of Gallus gallus var. domesticus according to the present invention comprises the steps of: preparing a concentrate by mixing Gallus gallus var. domesticus with medicinal herbs including Cirsium japonicum, jujube, Kalopanax septemlobus, Cudrania tricuspidata (Carr.) Bureau ex Lavallée, Achyranthes japonica, Atractylodes macrocephala Koidzumi, and Pueraria thunbergiana and then heating the mixture; freeze-drying the concentrate to prepare a powder; adding lactic acid bacteria to a mixed solution in which the powder is mixed with purified water, and then fermenting same; adding protease to the fermented product to hydrolyze same; and freeze-drying the hydrolysate to prepare a fermented extract of Gallus gallus var. domesticus.

The present invention belongs to the technical field of molecular biology, and particularly relates to a whole-genome chip for germplasm traceability of yellow-feathered broilers and its application. It is prepared from probes composed of 9,737 germplasm traceability sites of yellow-feathered broilers and 261 disease resistance-related sites, with a total of 9,998 SNP molecular markers. The physical position information on the chicken reference genome is shown in Table 1. The chip sites cover all chromosomes, are evenly distributed on each chromosome, with an average distance of 95 Kb, having the advantage of low marker density. The cost of the liquid-phase chip directly depends on the marker density, enabling genomic breeding related to chicken disease resistance at a relatively low cost. Moreover, the chip product has good stability, with a genotype consistency rate of >99% for repeated samples, and can reliably achieve germplasm traceability for local chicken breeds, commercial chicken breeds, and wild red jungle fowl populations at home and abroad, as well as commercial broilers of yellow-feathered broilers with highly similar population genetic backgrounds.

The present invention belongs to the technical field of nutritious food and protein hydrolysate, and specifically relates to a millet protein hydrolysate, a nutritional composition and its application in improving mental depression, anorexia, nausea and vomiting, gastrointestinal damage, leukopenia and erythropenia caused by tumors and radiotherapy and chemotherapy. The bioactive peptides contained in the millet protein hydrolysate of the present invention all have potential immunomodulatory effects. On this basis, the present invention also provides a nutritional composition containing the above-mentioned millet protein hydrolysate, and the millet protein hydrolysate is combined and used with Poria cocos, donkey-hide gelatin, Euryale ferox, roasted malt, chicken gizzard lining and tangerine peel, which can effectively improve mental depression, anorexia, nausea and vomiting, gastrointestinal damage, leukopenia and erythropenia caused by tumors and / or radiotherapy and chemotherapy, and improve the body's white blood cell count and hematopoietic ability.

The invention discloses a structural variation molecular marker related to Wenchang chicken feather color variation and application thereof, and relates to the field of gene detection, the structural variation marker sequence of the molecular marker is SEQ ID No: 01, the deletion site is on a Wenchang chicken reference genome ASM4043665v1, and the deletion site is Gallus gallus chick; the position of the probe in Genbank: GCA040436655.1 is CM080655.1: 9712333-9712763, and the probe in Genbank: GCA040436655.1 is CM080655.1 According to the present invention, the feather color containing the SEQ ID No: 01 structure variation marker sequence in the Wenchang chicken genome is deeper than the feather color of the individual without the SEQ ID No: 01 structure variation marker sequence, such molecular marker can be applied to the breeding detection kit of Wenchang chicken, and the powerful reference is provided for the directional breeding of the feather color.

The invention discloses a spleen-tonifying, appetite-promoting and dampness-removing therapy for mild chronic hepatitis, which comprises the following Chinese herbal medicine components in parts by weight: 15-20 parts of radix bupleuri, 10-15 parts of scutellaria baicalensis, 10-15 parts of codonopsis pilosula, 5-10 parts of liquorice, 8-12 parts of hawthorn, 5-10 parts of medicated leaven, 5-10 parts of malt, 3-6 parts of endothelium corneum gigeriae galli, 5-10 parts of dandelion, 2-5 parts of honeysuckle, 3-6 parts of ginger and 5-10 parts of jujube. The use method of the spleen-tonifying, appetite-promoting and dampness-removing therapy comprises the following steps: uniformly mixing the Chinese herbal medicines according to the formula proportion, putting the mixed Chinese herbal medicines into a decoction pot, adding 1200-1500ml of clear water, and soaking for 30 minutes to 1 hour; decocting with fire, braising with soft fire for 20 minutes when the water is decocted to about 1 / 3, turning off the fire, and filtering with gauze after the liquid medicine is cooled, so as to obtain the liquid medicine; the liquid medicine is added into a proper amount of warm water to be diluted before being taken every time, and 100-150 ml of the liquid medicine is taken with warm water in the morning and at night every time.

The present invention discloses a structural variation molecular marker related to the plumage color variation of Wenchang chickens and its application, which relates to the field of gene detection. The structural variation marker sequence of the molecular marker is whether there is a deletion mutation of the sequence shown in SEQ ID No: 01 in Wenchang chickens. The deletion site is on the Wenchang chicken reference genome ASM4043665v1, Gallus gallus chicken; the position in Genbank: GCA_040436655.1 is CM080655.1: 9712333-9712763. The present invention finds that the plumage color of Wenchang chickens containing the structural variation marker sequence of SEQ ID No: 01 is darker than that of individuals without the structural variation marker sequence of SEQ ID No: 01. Such a molecular marker can be applied to the breeding detection kit of Wenchang chickens, providing a strong reference for the directional breeding of plumage color.

The present invention provides a hawthorn and tuckahoe zinc oral liquid and a preparation method thereof, and belongs to the field of medical technology. The hawthorn and tuckahoe zinc oral liquid of the present invention is composed of the following raw materials in parts by weight: 0.5-5 parts of hawthorn, 0.5-5 parts of malt, 0.5-5 parts of tuckahoe, 0.5-5 parts of radish seeds, 0.1-3 parts of chicken gizzard lining, 0.0008-0.002 parts of zinc gluconate, 0.1-5 parts of sugar, 0.1-5 parts of honey, and 0.001-0.01 parts of stevioside. The hawthorn and tuckahoe zinc oral liquid prepared by the present invention can effectively improve the symptoms of decreased appetite, partial eating, malnutrition and the like caused by indigestion, improve the body's immunity, and ensure the body's nutritional needs.

The present invention discloses a method based on ARL13B The invention relates to a structural variation marker for Wenchang chicken breeding and a detection method thereof, and relates to agricultural bioengineering. The sequence of the structural variation molecular marker is SEQ ID No: 01, and the structural variation molecular marker is located on the Wenchang chicken gene reference genome ASM4043665v1, Gallus gallus chicken; the position of the structural variation molecular marker in Genbank: GCA_040436655.1 is CM080655.1: 108497195-108497244, and the structural variation molecular marker is a deletion site. At the same age, the weight of individuals lacking the structural variation marker sequence is significantly higher than that of individuals without the deletion. The molecular marker obtained by the invention can be applied to Wenchang chicken breeding.

This invention belongs to the field of animal cell culture technology, specifically relating to chicken embryo fibroblast cell lines, their culture media, acclimatization methods, and applications. This application provides a chicken embryo fibroblast cell line derived from… Gallus gallus The chicken embryo fibroblast suspension cell line CEF-P17 3D is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: C202661 and deposit date of March 31, 2026. This application also provides a culture medium for culturing the chicken embryo fibroblast cell line and a method for acclimatizing the chicken embryo fibroblast cell line, which shortens the acclimatization period, simplifies the operation, and is suitable for large-scale culture. This application also provides the application of the culture medium for the chicken embryo fibroblast cell line. Furthermore, this application also provides the application of the chicken embryo fibroblast cell line in the preparation of cultured meat.

The present invention belongs to the technical field of skin dressings. The present invention provides a skin repair and whitening dressing based on a black-skinned spruce extract and a preparation method. The preparation method comprises the steps of: preparing a modified sodium hyaluronate solution by mixing citrine pectin with sodium hyaluronate; encapsulating a black-skinned spruce water extract dry powder, a black-skinned spruce ethanol aqueous solution extract dry powder and a citrine peel water extract in liposomes; and then placing the liposomes in a modified sodium hyaluronate solution to prepare a hydrogel colloid to obtain a dressing. The skin dressing obtained by the present invention can significantly inhibit tyrosinase and has a good scavenging rate for DPPH free radicals, and has a positive effect on skin whitening; at the same time, it also has excellent long-term antibacterial properties and can be used repeatedly for a long time; and it is also conducive to the repair and healing of burned skin.

The present application belongs to the field of bioengineering, and particularly relates to a kind of recombinant yeast and its construction method, the supernatant protein factor derived from southern Peromyscus, Homo sapiens and / or Gallus gallus is expressed in the recombinant yeast, squalene monooxygenase, phospholipid synthesis transcription factor, and / or product synthase (especially beta-amyrin synthase, ergosterol synthase and / or lanosterol synthase), the recombinant yeast can efficiently synthesize triterpenoid compounds (especially squalene, beta-amyrin) and / or sterol compounds (especially ergosterol and / or lanosterol).

The invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating unstable carotid plaque and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following active pharmaceutical ingredients in parts by mass: 5-30 parts of salvia miltiorrhiza, 5-30 parts of endothelium corneum gigeriae galli, 10-50 parts of sun-dried ginseng, 3-30 parts of pseudo-ginseng, 3-15 parts of leech and 20-60 parts of pine needle. Clinical tests show that the traditional Chinese medicine composition has an anti-atherosclerosis effect in the aspect of reducing carotid plaque integral, and can effectively reduce total cholesterol and low-density lipoprotein level.