61results about How to "Conservation of Germplasm Resources" patented technology

The invention relates to a method for promoting the propagation of tricholoma matsutake artificially and belongs to the technical field of edible fungus germplasm resource protection and utilization, in particular to innovative technology which is used for realizing stable and high yield of the tricholoma matsutake by using 'tricholoma matsutake mountain' forestry management and adopting manual propagation promoting measures in a special environment needed for growing the tricholoma matsutake in order to improve the fact that the artificial culture of the tricholoma matsutake by using purely cultured stains is a goal difficult to fulfill for a long time. The technical scheme of the invention mainly comprises propagation promoting technology using tricholoma matsutake spores, mycorrhizae and hyphae as propagation materials, forestry management tricholoma matsutake propagation promoting technology and fungus shiro artificial tricholoma matsutake propagation promoting technology. The method not only is a tricholoma matsutake native area conservation and propagation promoting measure, but also can protect the shiro, promote the sound growth of the shiro and promote the yield and quality of tricholoma matsutake.

The invention discloses a tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima. According to the method, the improved MS, 6-benayl aminopurine (6-BA) and 3-indoleacetic acid (IAA) are used as an inductive culture medium for the cluster buds of Chongzuo camellia nitidissima; and the combination of the two growth hormones can be used for activating acting enzymes in the body cells of Chongzuo camellia nitidissima, and promoting the germination and the growth of the explants of Chongzuo camellia nitidissima in a proper concentration and a weakly acidic environment; the thick and strong cluster buds can be formed after the culturing for 55 days, and the propagation rate is increased by 20 times. According to the method disclosed by the invention, 1/2 improved MS and indolebutyric acid (IBA) or 1/2 improved MS and napthaleneacetic acid (NAA) are further used as an inductive culture medium for rooting, and capable of effectively promoting the single seedlings of Chongzuo camellia nitidissima to root to form new plants. Via the tissue culture and propagation method applying the three inductive culture mediums for Chongzuo camellia nitidissima, Chongzuo camellia nitidissima can be rapidly and effectively propagated, thus solving the problems that wild resources are diminished gradually and the natural propagation rate of Chongzuo camellia nitidissima is low, and effectively protecting the precious plant genetic resource which is rare in the world.

The invention relates to a breeding technology for large-size parents of Eriocheir sinensis. The breeding technology comprises the step of selecting the athletic parents of the Eriocheir sinensis, wherein the size of the female crab is above 150g and the size of the male crab is above 200g; the breeding technology is characterized by comprising the following steps: from the last ten-day of October to the first ten-day of November per year, soaking the selected parents of the Eriocheir sinensis into fresh water with tiny running water and sufficient oxygen for 2-3 minutes, and then taking out; after 3-5 minutes, disinfecting by using potassium permanganate; temporarily breeding in an indoor glass reinforced plastic over-wintering pool; starting the over-wintering breeding for the parents of the Eriocheir sinensis in the middle ten days of December; at the beginning of March per year, at a mating moment, controlling the water temperature at 9-12 DEG C, wherein the male and female ratio is (1.5-2):1 and the mating density is 7-13 pieces/m<2>; after oogenesis, continuously aerating and increasing the inflating volume, wherein the breeding temperature for the larva of the Eriocheir sinensis is at 24-25 DEG C and the breeding seawater salinity is 1.8%; and feeding rotifera, fairy shrimp larva and fresh water cladocerans biological baits.

The invention belongs to the technical field of rice planting, in particular to a rice yield increasing planting method which increases the yield through intervarietal mixed cultivation. According to the rice yield increasing planting method, rice varieties with different resistance properties are fully combined to form a stable ecological system suitable for rice to survive, and the high yield of rice is realized through potential maximization. According to the rice yield increasing planting method, rice varieties with similar growth periods, similar agronomic traits and different resistance properties are selected for raise rice seedlings, and then the rice seedlings are mechanically transplanted at the same period. The different resistance properties of the rice varieties interact to give full play to the production potential of all the rice varieties; meanwhile, a three-dimensional community is built, so that stability of a field ecological system is improved, common diseases, pests and weeds are effectively controlled without applying pesticides or applying a small number of pesticides, and green production of rice is realized.

The invention belongs to the technical field of cultivation of Gymnocypris przewalskii, and in particular relates to a cultivating method of Gymnocypris przewalskii spring fingerlings. The scheme of the method comprises the four steps of changing feeding points, managing water quality, using a feeding method and cultivating spring fingerlings. Until June, the cultivated spring fingerlings can reach 9-11 cm in size and 7.0-8.0 g in weight, are 1+ age Gymnocypris przewalskii spring fingerlings, can be purchased or proliferated for releasing and can also enter the 2+ age fingerling cultivation stage. The method provides powerful support for the cultivation of Gymnocypris przewalskii, the germ plasm resource protection of Gymnocypris przewalskii, satisfaction of the market demand of Gymnocypris przewalskii, and the cultivation of Gymnocypris przewalskii in fresh water of cold areas. The method has the advantages that: being different from the cultivation modes of spring fingerling breeding of other cultured fishes, cultivation in the original pond is adopted; the strongly-cultivated fingerlings have high survival rate and less diseases; and simpleness and convenience for operation and labor saving are achieved.

The invention belongs to a new plant or a method for obtaining a new plant and the technical field of plant regeneration by tissue culture technology, and discloses an in-vitro propagation method of plant Hydrocera triflora. The in-vitro propagation method of the plant Hydrocera triflora comprises seed collection and disinfection; seed germination and bud induction culture; bud proliferation subculture; rooting and strong seedling culture. The method has great advantages: 1. a small amount of seeds are needed; 2. the proliferation coefficient is large, and the bud proliferation coefficient reaches 8.00 in 30 days of culture; 3. rooting and strong seedlings are good, the rooting rate is 100%, and the number of roots is as high as 6.42; 4. a developed medium has a simple formula and a wide range of ingredients, and is easy to operate and formulate; 5. the breeding of seedlings can be produced annually without being limited by time and seasons.

The invention discloses a three-grade branch pruning technology of fructus evodiae. The three-grade branch pruning technology comprises the following steps: carrying out tip pruning on branches near the ground of fructus evodiae young trees with the plant spacing of 3*4m and planted for 1-2 years; controlling the branches to be dwarfed so that primary braches are gradually divided into stem branches; carrying out tip pruning on the stem branches after autumn or spring to form secondary branches; finally, carrying out tip pruning to form tertiary branches; pruning the secondary branches and the tertiary branches; pruning dense branches, overlapped branches, downward branches, insect branches and the like; and continuously pruning and managing and enabling whole plants to form tree shapes which have short stems and low crowns, circular outsides and hollow insides and wide tree crowns, are ventilated and transparent, and have the tree heights controlled to be lower than 1.5m and the tree crown coverage of being more than 10 square meters within 3-4 years. According to the three-grade branch pruning technology, the fructus evodiae can be subjected to three grades of branch pruning so that the quality and yield of cultivation of the fructus evodiae are effectively improved and the labor cost is reduced; the germplasm resources of the fructus evodiae are effectively protected; the three-grade branch pruning technology has remarkable social and economic benefits and has a wide application prospect.

The invention discloses a quick red bean local variety purifying and rejuvenating method and relates to the technical field of crop variety resource protection and utilization. The method comprises the steps: performing large group selection to select single plants and performing plant laboratory test and forming plant rows; identifying and comparing multiple plant rows and forming plant lines through the plant laboratory test; comparing multiple plant lines to predict yield and selecting high-yield plant systems through the plant laboratory test; performing mixed line breeding and producing qualified seeds. The method disclosed by the invention widens selected groups, can perform plant laboratory test according to red bean representations, ensures population genetic stability, reduces gene loss generation, quickly increases population quantity and increases output quantity of breeder seeds; seed propagation generations are reduced, a mixing opportunity is reduced, and a natural selection effect is reduced; meanwhile, a red bean local variety purifying and rejuvenating process is quickened, excellent variety characteristics are kept, germplasm resources with local characteristics are effectively protected, and the breeder seeds can be directly applied to large field production or the breeder seeds can be utilized to directly produce seeds.

The invention relates to an amplification primer of a psammobiidae shellfish mitochondria COI (Cytochrome Oxidase I) gene. 64 psammobiidae sequences obtained by amplification of universal primers are subjected to comparative analysis by using CLUSTALW software; a plurality of pairs of primers are designed by using primer design software Primer Premier 5 in a determined conserved region; PCR (Polymerase Chain Reaction) reaction experiments are repeatedly performed by using 200 psammobiidae individuals under a 10 mu system; and finally an efficient amplification primer of a psammobiidae shellfish mitochondria COI gene sequence is sifted through detection of agarose gel electrophoresis. The amplification primer is characterized in that COIZGF is 5'-ACAAATCATTAAGGATATTGG-3'; and COIZGR is 5'-GTCCGAAGAATCAAAACAAATG-3'. With the adoption of the primer of the psammobiidae shellfish mitochondria COI, provided by the invention, target segments of different species of psammobiidae shellfishes can be effectively amplified, the amplification efficiency is more than 90%, the primer can satisfy the demands of genetic diversity research on psammobiidae, and plays an important role in the aspects of protection and utilization of psammobiidae resources, diversity assessment, genetic breeding and the like.

The invention discloses a lepista nuda strain and a sporocarp cultivation method thereof. The invention provides Lepista nuda(Lepista nuda DCH 560), and the deposit number is CGMCC No. 15376. The Lepista nuda DCH 560 (CGMCC No. 15376) is collected from a specimen isolated from the specimen of Dandong Kuandian Manchu Autonomous County, Liaoning Province, China. The strain can be obtained by artificial domestication. The sporocarp cap obtained by cultivation is purple, the flesh is thicker, the pleats are purple, and it has a strong aroma, which is consistent with the wild sporophore. The invention has obtained thesporophore of the lepista nuda and developed a new rare edible fungus, which is of great significance for protecting the germplasm resources of the species, enriching the edible mushroom variety resources, enriching the vegetable basket and the like. At the same time, the bacterium is a typical grass rot fungus and the crop stalks can be utilized, which has ecological significance.

The invention provides a novel method for breeding kelp seedlings with a gametophyte cloning method, comprising the following steps of: respectively separating a female cell on a female gametophyte and a male cell on a male gametophyte under a microscope by using a capillary tube separation technology; then respectively cloning the female cell and the male cell by using a cloning vegetative propagation technology to respectively grow the female cell and the male cell into female and male gametophytes; continually breeding the gametophytes; when the gametophytes are bred to a fixed amount, damping a formed cell cluster by a cell stamping machine; mixing the female and male gametophytes and respectively releasing ova and sperms by the female and male gametophytes to spontaneously fertilize;putting fertilized zygotes into nutritive salt to be cultured; and after 10 weeks, obtaining seedlings with the height of about 1.5 cm. According to the invention, a fertilization process and the control of temperature and illumination are realized by manual operation, so that the growth period of offspring seeds is greatly shortened; meanwhile, the female cell and the male cell are separated from the gametophytes by using the capillary tube separation technology to clone and culture, so that the quality and the yield of the seedlings are improved, the cultivation of the kelp can be satisfied; and by utilizing the novel method, the cost for breeding the kelp seedlings is low.

The invention relates to an industrialized production method for edible fungi, belongs to the field of agricultural biotechnologies, and particularly relates to an industrialized production method fortricholoma matsutake edible fungi. The method comprises the following steps: strain production, material preparation, material steaming, cooling, inoculation, bagging, management in a fungus growth period, management in the process of producing the edible fungi, and performing harvesting. The method provided by the invention realizes artificial domestication of tricholoma matsutake mother strains, completely changes a current situation that tricholoma matsutake production completely depends on nature, industrially produces the edible fungi, and realizes stable production, a high yield and excellent quality of tricholoma matsutake products; the method effectively protects the tricholoma matsutake germplasm resources and a living ecological environment on which the tricholoma matsutake germplasm resources depend, and provides an effective way for sustainable development of tricholoma matsutake production; and the method has outstanding economic and social benefits.

Provided is an accelerated breeding method for licorice seeds. The licorice fruit bearing period is shortened, and enough seeds are provided for artificially planting licorice. The accelerated breeding method for licorice seeds comprises the steps that triennial licorice principle roots are taken as licorice seedlings; a land parcel with irrigation arable soil or sandy soil is selected; the licorice seedlings are planted in a wide and narrow row spacing planting method; after the licorice seedlings are planted, a drip irrigation zone is laid beside a licorice seedling zone, and covered with a black mulching film; in the key breeding period of the licorice seedlings, drip irrigation water is applied once every 20 days, the drip irrigation amount is 20 m<3> each mu each time, and the number of total drip irrigation times in a year is 8-10; after 80% licorice pods in the licorice seed land are mature, a hay mower is used for harvesting at a time, and threshing and airing are carried out in time. By means of the breeding method, the breeding period of licorice can be 2-3 years earlier, the seed yield is increased by 2 times or above, the worm damage rate of the seeds is controlled to be within 5% and is reduced by 15% or above compared with that of wild seeds, the seed germination rate is greatly increased, and the seed germination potential is greatly improved. Meanwhile, the pressure that licorice seeds rely on wild seeds for a long time can be relieved, the wild licorice genetic resource can be protected, and artificial destruction on the environment is controlled.

The invention discloses a fish breeding and catching method for ecological circulation type fishery, and belongs to the field of fishery; the fish breeding and catching method for ecological circulation type fishery comprises the steps, for example: selecting water areas, arranging fish blocking facilities, cultivating fingerlings, discharging the fingerlings out of ponds, breeding the fingerlingson water and recapturing the bred fingerlings. The survival rate of the fingerlings can be effectively increased after the fingerlings leave the ponds and enter the field environment; the fishery resources can be restored, the fish germplasm resources can be protected, and stable income is created for fishermen; the method has the advantage of simultaneous protection and utilization. According tothe method, the degraded fish resources of the lakes can be updated, the fish population structures are enriched, the total amount of the fish resources is increased, the sustainable utilization of the commodity fish resources is guaranteed, the eutrophication level of the lakes is reduced, and the ecological circular development of fishery is facilitated.

The invention provides a cymbidium sinense 'labellum and petal sepalody' character-related functional molecular marker. The functional molecular marker is M4wt or M4m, the nucleotide sequence of the M4wt is shown in SEQ ID NO: 1, and the nucleotide sequence of the M4m is shown in SEQ ID NO: 2. By separating and comparing related gene sequences of multiple varieties of cymbidium sinense, a functional specificity site, which is different from other varieties of cymbidium sinense, in 'labellum and petal sepalody' varieties 'Lvyun' and 'Taiyanghua' is obtained. Based on difference of the site, three specific primers M4F1, M4F2 and M4R are designed. A primer pair of M4F1 and M4R and a primer pair of M4F2 and M4R are utilized to amplify the total DNA of the genome of the cymbidium sinense, then agarose gel electrophoresis detection is performed, and thus the 'labellum and petal sepalody' varieties can be quickly identified. The method provided by the invention has great value and meaning in identification and protection of germplasm resources of cymbidium sinense, molecular marker-assisted breeding and molecular design breeding.