Technique for processing plant under low temperature by polygonum cuspidatum callus

A callus and low temperature treatment technology, applied in the field of Polygonum cuspidatum plants, can solve the problems of restricting the preservation of Polygonum cuspidatum germplasm resources, etc., and achieve the effects of protecting germplasm resources and reducing the economic cost of preservation.

Inactive Publication Date: 2017-01-25
李志勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on cryopreservation of Polygonum cuspidatum callus, which seriously restricts the preservation of Polygonum cuspidatum germplasm resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] (1) Treatment of explants: select Polygonum cuspidatum seeds harvested in the same year, gently scrub with detergent water, rinse with tap water for 12 minutes, soak in clean water for 9 hours, then transfer to 52 mg / mL gibberellin solution and soak for 12 hours, Then soak in 75% ethanol solution for 12 seconds in the ultra-clean workbench, rinse with sterile water for 5 times, then disinfect with 0.1% mercuric acid solution containing 0.01% Tween-20 for 6 minutes, rinse with sterile water for 4 times, and then use sterile filter paper Blot the moisture from the surface and set aside.

[0025] (2) Callus induction: Use a sterile scalpel to cut a knife on the seeds treated in step (1), inoculate them on the induction medium for callus induction culture, and carry out at 26°C after inoculation Culture in total darkness until callus formation. The induction medium is: MS+ 5mg / L 2,4-D+ 2mg / L 6-BA+ 0.6mg / L KT+3.0% sucrose+0.6% agar+0.06% activated carbon, the pH value is 5....

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PUM

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Abstract

The invention discloses a technique for processing plant under low temperature by polygonum cuspidatum callus. A callus obtained by induction of a polygonum cuspidatum seed is used as a material, an in-vitro ultra-low temperature storage technique system of polygonum cuspidatum is set up, thus high-quality germplasm resource of polygonum cuspidatum is effectively protected. Through callus induction, pre-cultivation, loading, dehydration, cooling, washing, re-cultivation and regeneration, and other processes, the ultra-low temperature storage of the polygonum cuspidatum callus is realized; the technique can be used as a method for stably storing germplasm resources for a long term, so as to reduce the economic cost for storage, and effectively protect rare germplasm resources.

Description

technical field [0001] The invention relates to the technical field of Polygonum cuspidatum plants, in particular to a technique for treating plants of Polygonum cuspidatum callus at low temperature. Background technique [0002] Polygonum cuspidatum is a Polygonaceae plant, and the dried rhizomes and roots of Polygonum cuspidatum (PoLygonum cuspidatum Sieb.et zuce). It is mainly produced in Jiangsu, Zhejiang, Guangdong, Guangxi, Sichuan, and Guizhou. Excavated in spring and autumn, remove residual stems and fibrous roots, wash the soil, cut into slices, and dry in the sun. Rhizomes or roots are in the form of round grain-shaped segments, branched, slightly curved, and of different lengths. The outer surface is brown, with obvious longitudinal wrinkles, fibrous roots and racemose fibrous root scars. The texture is hard, the broken surface is brownish yellow, fibrous. Section skin is thin, brown. Main ingredients: Contains anthracene derivatives, mainly in free form, inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N3/00
CPCA01H4/008A01H4/001A01N3/00
Inventor 李志勇
Owner 李志勇
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