5results about How to "Excellent Germplasm Resources" patented technology

The application discloses a kind of AbGLA1 The application discloses application of a gene in improving protein content of Agaricus bisporus, and belongs to the technical field of edible mushroom breeding. AbGLA1 The nucleotide sequence of the gene is shown as SEQ ID NO.1 or SEQ ID NO.2, and by overexpressing the gene, the protein content of fruiting bodies of Agaricus bisporus can be significantly improved. AbGLA1 The key role of the gene in protein content regulation is verified. Not only excellent germplasm resources for high-protein Agaricus bisporus breeding are provided, but also a key target for genetic improvement of protein content traits is determined.

The invention discloses a cultivation method and a cultivation evaluation method of Amomum villosum stem tiller seedlings, and relates to the field of cultivation evaluation methods. The cultivation method comprises the following steps: adding 6-benzyladenine into a culture medium to obtain an induced budding culture medium; a tissue culture rapid propagation technology is adopted, leaves and sheaths are cut off, and stems with roots are left; and vertically inserting the stem segments into an induction budding culture medium, and culturing to obtain stem tillering seedlings. The rapid tissue propagation technology is adopted, pathogens and other viruses in the plant body can be effectively removed, the 6-benzyladenine with the high concentration is adopted to induce budding of stem segments, meanwhile, the vertical inoculation mode is combined, budding and proliferation of stem tillering seedlings are effectively improved, the seedling growing period is short, high-speed and high-yield high-quality seedlings can be achieved, and the method is suitable for large-scale popularization and application. And excellent germplasm resources required by industrial development are ensured.

The invention relates to a corn internode length regulation gene ZmIL1, and an expression product, an excellent haplotype and application thereof in corn breeding, and belongs to the technical field of genetic engineering breeding. The nucleotide sequence of the gene ZmIL1 is as shown in SEQ ID NO. 1. According to the expression product of the corn internode length regulation gene ZmIL1, the expression product of the gene ZmIL1 is a protein coded by the gene ZmIL1, and the amino acid sequence of the protein is as shown in SEQ ID NO. 2. According to the four haplotypes of the corn internode length regulation gene ZmIL1, the nucleotide sequences of the four haplotypes are as shown in SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6, and the SEQ ID NO.5 is an excellent haplotype. The invention further provides application of the gene ZmIL1 for regulating and controlling the internode length of the corn in genetic breeding of the corn and application of the gene ZmIL1 in corn production in Huang-Huai-Hai regions of China. The application method of the corn internode length regulation gene ZmIL1 in corn breeding practice can be popularized and applied to other related genes.

The application discloses a KASP molecular marker for identifying pepper leaf occurrence and application thereof. Through construction of a genetic map linked with a pepper leaf trait and development of a molecular marker, the strongest candidate gene for controlling the pepper leaf trait is determined, a KASP marker and a primer for identifying pepper leaf occurrence are obtained through research, and the KASP marker and the primer can be used for detecting or screening different pepper leaf types, identifying pepper leaves, and performing pepper leaf type typing. Meanwhile, the application provides a method for typing pepper leaves or breeding pepper normal leaf plants, without needing to perform a large amount of genotype sequencing analysis, and different leaf type plants can be identified and distinguished through routine detection amplification in a laboratory, so that the method is convenient and easy to operate, the screening and identification of pepper leaf occurrence can be performed in advance, the breeding screening time is shortened, more excellent pepper germplasm resources are provided, and technical support is provided for pepper germplasm identification and strain breeding.

This invention belongs to the field of tissue culture technology and provides a method for rapid propagation of Tibetan Saussurea involucrata via tissue culture, comprising the following steps: From May to July, Tibetan Saussurea involucrata explants are subjected to stepwise sterilization to obtain sterile explants; these explants are inoculated into a starter culture medium for seed germination to obtain callus tissue; the callus tissue is transferred to MS proliferation medium containing 2.0-3.0 mg / L 6-BA + 0.05-0.1 mg / L IAA, and cultured for 21-25 days. TDZ is then added to promote shoot differentiation, while simultaneously reducing IAA to 0.01-0.02 mg / L, and cultured for another 21-25 days to obtain rootless seedlings; these seedlings are then transferred to rooting medium and cultured for 21-25 days to obtain tissue culture seedlings; after hardening off the seedlings for 2-4 days, they are transplanted into a sterile substrate. This method significantly improves the propagation stability and seedling quality of Tibetan Saussurea involucrata via tissue culture by quantifying key process parameters and establishing a multi-dimensional evaluation system.