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This invention belongs to the field of tissue culture technology and provides a method for rapid propagation of Tibetan Saussurea involucrata via tissue culture, comprising the following steps: From May to July, Tibetan Saussurea involucrata explants are subjected to stepwise sterilization to obtain sterile explants; these explants are inoculated into a starter culture medium for seed germination to obtain callus tissue; the callus tissue is transferred to MS proliferation medium containing 2.0-3.0 mg / L 6-BA + 0.05-0.1 mg / L IAA, and cultured for 21-25 days. TDZ is then added to promote shoot differentiation, while simultaneously reducing IAA to 0.01-0.02 mg / L, and cultured for another 21-25 days to obtain rootless seedlings; these seedlings are then transferred to rooting medium and cultured for 21-25 days to obtain tissue culture seedlings; after hardening off the seedlings for 2-4 days, they are transplanted into a sterile substrate. This method significantly improves the propagation stability and seedling quality of Tibetan Saussurea involucrata via tissue culture by quantifying key process parameters and establishing a multi-dimensional evaluation system.