3results about How to "Excellent germplasm resources" patented technology

The application discloses a kind of AbGLA1 The application discloses application of a gene in improving protein content of Agaricus bisporus, and belongs to the technical field of edible mushroom breeding. AbGLA1 The nucleotide sequence of the gene is shown as SEQ ID NO.1 or SEQ ID NO.2, and by overexpressing the gene, the protein content of fruiting bodies of Agaricus bisporus can be significantly improved. AbGLA1 The key role of the gene in protein content regulation is verified. Not only excellent germplasm resources for high-protein Agaricus bisporus breeding are provided, but also a key target for genetic improvement of protein content traits is determined.

The application discloses a KASP molecular marker for identifying pepper leaf occurrence and application thereof. Through construction of a genetic map linked with a pepper leaf trait and development of a molecular marker, the strongest candidate gene for controlling the pepper leaf trait is determined, a KASP marker and a primer for identifying pepper leaf occurrence are obtained through research, and the KASP marker and the primer can be used for detecting or screening different pepper leaf types, identifying pepper leaves, and performing pepper leaf type typing. Meanwhile, the application provides a method for typing pepper leaves or breeding pepper normal leaf plants, without needing to perform a large amount of genotype sequencing analysis, and different leaf type plants can be identified and distinguished through routine detection amplification in a laboratory, so that the method is convenient and easy to operate, the screening and identification of pepper leaf occurrence can be performed in advance, the breeding screening time is shortened, more excellent pepper germplasm resources are provided, and technical support is provided for pepper germplasm identification and strain breeding.

This invention belongs to the field of tissue culture technology and provides a method for rapid propagation of Tibetan Saussurea involucrata via tissue culture, comprising the following steps: From May to July, Tibetan Saussurea involucrata explants are subjected to stepwise sterilization to obtain sterile explants; these explants are inoculated into a starter culture medium for seed germination to obtain callus tissue; the callus tissue is transferred to MS proliferation medium containing 2.0-3.0 mg / L 6-BA + 0.05-0.1 mg / L IAA, and cultured for 21-25 days. TDZ is then added to promote shoot differentiation, while simultaneously reducing IAA to 0.01-0.02 mg / L, and cultured for another 21-25 days to obtain rootless seedlings; these seedlings are then transferred to rooting medium and cultured for 21-25 days to obtain tissue culture seedlings; after hardening off the seedlings for 2-4 days, they are transplanted into a sterile substrate. This method significantly improves the propagation stability and seedling quality of Tibetan Saussurea involucrata via tissue culture by quantifying key process parameters and establishing a multi-dimensional evaluation system.