A kind of breeding method of high temperature resistant sea cucumber strain
A technology of high temperature resistance and sea cucumber, applied in application, climate change adaptation, fish farming and other directions, can solve the problems of sea cucumber impact, long duration, and high water temperature in sea cucumber ponds, achieve stable high temperature resistance properties, and improve economic benefits. , the effect of improving the selection efficiency
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[0019] The present invention is specifically introduced below in conjunction with specific embodiment:
[0020] 1) Apostichopus japonicus parents are surviving individuals collected from ponds in Yantai, Qingdao, and Dongying after high temperature elimination in summer. The pond water temperature is above 32°C and the high temperature lasts for 5 days, and the survival rate of elimination is <0.05%. 100 heads of each of the three groups were used as parents, and were bred to sexual maturity. The method of artificial cold water drainage was used to breed ginseng. Based on the principle of 1 female ginseng and 1-3 male ginseng, more than 60 full-sib families and half-sib families were established. 72 families were established, and the breeding tanks of each family were isolated and cultivated;
[0021] 2) Each family of sea cucumbers develops to the stage of ear-shaped larvae. Take 1 L of sea cucumber larvae (density 0.5 inds / mL) from each family in a glass beaker, place them i...
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