Method for breeding sexual triploids of colored zantedeschia by using 2n pollen

A calla lily and triploid technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of few excellent germplasm opportunities, long time for triploid breeding, and inability to reflect heterosis, etc. The effect of increasing the induction rate

Inactive Publication Date: 2011-10-19
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects and deficiencies in the prior art that the color calla lily triploid breeding time is long, can not reflect heterosis, and has few chances of further creating excellent germplasm, and its purpose is to provide a 2n pollen for breeding color Method of Sexual Triploidy of Calla Lily

Method used

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Examples

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Embodiment Construction

[0038] The present invention will be further described below in conjunction with the examples, but this does not constitute a limitation to the protection scope of the present invention.

[0039] The chemical reagents used in the examples are all commercially available products, and the microscope is an optical microscope with a magnification of 100 times.

[0040] The formula of lactophenolic acid fuchsin solution belongs to the existing conventional formula, and the formula of lactophenolic acid fuchsin solution is: 20g phenol + 20ml 90% lactic acid + 40ml 98% glycerin + 20ml water + 80mg acid fuchsin.

[0041]The formula of the improved phenol fuchsin staining solution is: stock solution A: take 3g of basic fuchsin and dissolve it in 100ml of 70% alcohol (can be stored for a long time); stock solution B: take 10ml of solution A and add 90ml of 5% phenol (that is, carbolic acid) Use within 2 weeks); stock solution C: take 55ml of solution B, add 6ml of glacial acetic acid an...

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Abstract

The invention discloses a method for breeding sexual triploids of colored zantedeschia by using 2n pollen, and belongs to the technical field of plant ploidy breeding. The method comprises the following steps of: (1) determining a 2n pollen induced appropriate development stage; (2) preparing styles to be treated; (3) inducing the 2n pollen by colchicine treatment; (4) identifying the 2n pollen; (5) collecting the 2n pollen; (6) performing sexual polyploidy hybridization; and (7) identifying sexual triploid descendants. By the method, the sexual triploids of the colored zantedeschia can be successfully obtained in one step.

Description

technical field [0001] The invention belongs to the technical field of plant ploidy breeding, and in particular relates to a method for cultivating triploids through induced hybridization of plant 2n pollen. Background technique [0002] Colored calla lily (Coloured Zantedeschia spp) is a member of the Araceae family (Araceae), the genus Calla ( Zantedeschia ) 6 original species in the Aestivae group, and their hybrids ( Z. antedeschia hybrida ) collectively. The 6 original species are: Z. rehmnnii , Z. jucunda , Z. elliotiana , Z. pentlndii , Z. albomaculata ,and Z. valida . Cultivated species are mainly from Calla lily ( Z. elliottiana ), Silver Star Calla Lily ( Z. albomaculata ) and Calla Lily ( Z. rehmannii ) are cultivated. Colorful calla lilies are gorgeous in color and elegant in shape. In addition to being used as cut flowers and potted flowers, they can also be tied into bouquets and flower baskets. They are also unique materials for flower ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08
Inventor 吴红芝周涤李洁筠石景峰杨辉
Owner YUNNAN AGRICULTURAL UNIVERSITY
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