145results about How to "Shorten the breeding process" patented technology

The invention discloses a method used for quickly breaking dormancy of a peony seed epicotyl. With the method, the dormancy of the peony seed epicotyl supposed to naturally germinate in two years can be quickly broken, and the peony seed can germinate smoothly after being processed for three months; under the isolated culture condition, the dormancy characteristic of the peony seed epicotyl can be broken after the processing period of two weeks. With the adoption of the two methods disclosed by the invention, high-quality peony seedlings can be obtained; the peony nursery stock germinating quickly after processing is highly consistent with the phenotype of peony seedlings obtained by the traditional method, in other words, the parental genetic phenotype is maintained, and the seed germination, seedling and flow formation processes are greatly shortened; besides, the method can be used for creating heterogeneous parental new germplasm, so that the efficiency of traditional breeding and cross breeding of peony is improved, and the breeding and genetic improvement processes of peony are shortened.

The invention discloses a co-separation molecular marker TCR540 of a clubroot resistant CRb gene of Chinese cabbages. A nucleotide sequence of the co-separation molecular marker TCR540 is represented as SEQ ID NO.1, TCR540 and the CRb gene are co-separated, and TCR540 can be applied to molecular marker assisted selection of the clubroot resistant CRb genes of Brassica including Chinese heading cabbages and green pakchoi. The markers are used simultaneously in an assisted selection process, the theoretical accuracy of the selection can be up to 100%, and the co-separation molecular marker TCR540 is good in repeatability, high in reliability and low in detection cost and saves time and labor.

The invention discloses an SNP marker related to growth traits of black-bone chickens and an application thereof. The invention firstly provides an application of a substance for detecting polymorphisms or genotypes of an SNP marker rs317682933 in identification or auxiliary identification of chicken body weight. The SNP marker rs317682933 is the 73209189th nucleotide of 4th chromosome located ingalGalGal6-edition chicken genome, and the alleles of the marker are C and T. The invention further provides a method for identifying or assisting in identifying chicken body weight. The invention discovers that; when the genotype of the SNP marker rs317682933 in the chicken genome is CC, the body weight is larger than that of chickens whose genotype is CT, and the correlation between the marker and the body weight is gradually enhanced along with the increase of the age of the chickens. Therefore, the application of the SNP marker rs317682933 in the growth performance breeding of the Lueyangblack-bone chickens can improve the selection accuracy, increase the selection reaction and shorten the breeding process.

The invention discloses a method for breeding sexual triploids of colored zantedeschia by using 2n pollen, and belongs to the technical field of plant ploidy breeding. The method comprises the following steps of: (1) determining a 2n pollen induced appropriate development stage; (2) preparing styles to be treated; (3) inducing the 2n pollen by colchicine treatment; (4) identifying the 2n pollen; (5) collecting the 2n pollen; (6) performing sexual polyploidy hybridization; and (7) identifying sexual triploid descendants. By the method, the sexual triploids of the colored zantedeschia can be successfully obtained in one step.

The invention discloses a molecular marker of a high erucic acid gene in Brassica napus L. and a breeding method thereof and relates to the biological breeding field. The method comprises the following steps of: S1, constructing a segregating population; 2, extract that genomic DNA of the material to be tested by the CTAB method; 3, carry out PCR amplification; S4, using marker primer pairs Pb1, Pb2, Fa1, Fa2 to detect whether a single plant in the segregated population contains root swelling resistance site PbBa8.1 or low erucic acid gene fae1 and classify; S5, a new rapeseed variety with high erucic acid and root swelling resistance locus PbBa 8.1 and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and low erucic acid and high erucic acid and low erucic acid were selected. The method for selecting and breeding rapeseedwith root swelling disease resistance and low erucic acid in grain can be used for auxiliary selection of root swelling disease resistance site PbBa8.1 and low erucic acid gene fae1 with genetic linkage at seedling stage, The theoretical accuracy of gene-specific markers reached 100%, which improved the breeding efficiency of rapeseed resistant to root swelling and low erucic acid, and shortenedthe breeding process.

The invention discloses a KASP labeled primer for detecting a Wx-D1 gene in waxy wheat K107Wx1 and application of the KASP labeled primer, and belongs to the technical field of molecular genetic breeding; and three groups of KASP primers provided by the invention, when used for detecting the Wx-D1 genotype in the waxy wheat K107Wx1, are low in human error and high in analysis throughput, and are suitable for detecting a great amount of samples. A codominant molecular marker provided by the invention, when applied to transfer breeding of the Wx-D1 gene in the waxy wheat K107Wx1, has an important practical significance for accelerating genetic improvement by virtue of the Wx-D1 gene in the waxy wheat K107Wx1 and f improving a breeding efficiency.

The invention discloses a protein related to pollen grain germination and/or pollen tube growth, the encoding genes and the application thereof. The protein is the following protein shown in (1) or (2): (1) the protein being composed of amino acid sequences shown in sequence two of a sequence table; (2) the protein comprises the amino acid in the sequence two of the sequence table substituted and/or lacked and/or added and is related to the stress tolerance of plant. The protein and the encoding gene thereof related to the pollen grain germination and/ or pollen tube growth can be used for the genetic transformation research of the plant, increases the pollen germination rate of plant and/or the growth speed of the pollen tube and the reproductive efficiency of the plant, shortens the breeding process, has important theoretical and practical actual meaning for adjusting and controlling the reproductive development and gene engineering breeding of woody plants with slow growth development and accelerating the genetic improvement of the woody plants.

The invention provides a method for identifying a self-sterile S-gene type SSR molecular marker of a sweet cherry variety. The method uses PTCR4SSR to mark, wherein the SSR primer sequence is upstream primer 5'-CATAGGTTCAAACCATACCCGTG-3' and downstream primer 5'-CTCATCTTTGTAGGGTATAATACC-3' to amplify the DNA of sweet cherry of different varieties (systems), if the amplified fragment of 255bp can be amplified, pollen S-gene exists, and a electrophoretogram can be obtained by agar gel electrophoresis; according to the agarose elelctrophoretogram, the self-sterile gene type of each variety (system) can be determined, wherein the same bands are the same S-gene type, otherwise being different S-gene types. The SAM method is used to develop SSR marked PTCR4 in sweet cherry for identifying the S-gene type of different sweet cherry self-sterile gene type varieties (systems) and guiding selection of breeding parents and configuration of pollination varieties thereof, so that the detection efficiency of the self-sterile gene type can be improved, the breeding process of the sweet cherry is shortened, and the production cost is saved.

The invention relates to a method of a molecular authenticating technique for the indica-japonica of old species of Yunnan Rice, which belongs to the breeding field in the technical field of agricultural biotechnology. The technical proposal is as follows: five grains of the material seeds to be authenticated are taken for germinating under the temperature of 20 to 35 DEG C; after the seeds geminate for five days, the leaves are taken for extracting the total DNA of the rice; the concentration of the DNA is detected and the DNA is quantificationally diluted to 25ng/ul; a specific primer JI designed according to the sequence of the specific long-arm section of the 10th chromosome of the rice is used for carrying out PCR reaction; gel electrophoresis is carried out on the 2 percent agarose (containing EtBr0.1ug/ml); the PCR product is detected on a UV transilluminator; and the indica-japonica characteristics of the material are authenticated according to the electrophoresis result. The indica type rice has two PCR products with the molecular weights being respectively 280bp and 380bp; and the japonica rice has one PCR product with the molecular weight of 280bp, thus authenticating the indica-japonica characteristics of the old species of Yunnan Rice. The method has the effects of being quickly and effectively authenticating the indica-japonica characteristics of the old of Yunnan Rice.

The invention belongs to the field of detection of genetically modified plants, and particularly discloses 2 molecular markers for identifying the specificity of bivalent herbicide resistance gene cotton strains GGK2 and derived lines. Each molecular marker consists of nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention further discloses specific primers for amplifying the molecular markers, and purposes of the molecular markers and the specific primers. Accurate and reliable molecular markers and specific primers are provided for detection and identification of the bivalent herbicide resistance gene cotton strains GGK2 or derived materials thereof, and transgenic safety is guaranteed; then the detection method is simple, the detection speed is high, large-scale identification and screening are appropriately performed on homozygosis/heterozygote breeding materials in bivalent herbicide resistance genes in filial generations in heredity and breeding of genetically modified crops, shortening of a breeding process is facilitated, and the breeding cost is reduced.

The invention discloses a rapid breeding method of potatoes. Screening is conducted by methods of screening in Shandong and Inner Mongolia as well as screening spring, summer and autumn, varieties suitable for a central plain two-season crop area and a northern one-season crop area simultaneously can be selected, and potato new varieties only suitable for the central plain two-season crop area toplant or only suitable for the northern one-season crop area to plant also can be selected, so compared with the traditional breeding method, the rapid breeding method of the potatoes has the following advantages: the prepared hybridized combination is completely utilized and waste of hybridized materials is avoided; and through generation adding planting of two places, the operation from preparation of hybridized combination in the first year to variety registration totally needs 11 years, the breeding year limit is shortened and the breeding flow is accelerated, so that the pace of pushing the new varieties to the market is accelerated.

Provided is a method for breeding seeds of polygonatum cyrtonema Hua. The method comprises the following steps of: (1) putting the seeds of polygonatum cyrtonema Hua in a water-containing bucket for immersion and fermentation, wherein the fermentation time is 25-35 days, and the water temperature is 25-29 DEG C; (2) cleaning the fermented seeds of polygonatum cyrtonema Hua with clear water, layingthe cleaned seeds of polygonatum cyrtonema Hua on a culture medium, and then performing cold storage on the culture medium in an aerobic-environment refrigerating room, wherein the time of cold storage is 25-35 days, the temperature of the refrigerating room is 2-6 DEG C, the moisture content of the air in the refrigerating room is 11-18%, and the refrigerating room is internally provided with acold-light lamp; and (3) sowing the refrigerated seeds obtained in the cold environment to loose soil for seedling cultivation. According to the method for breeding the seeds of polygonatum cyrtonemaHua, not only can a high germination rate of the seeds of polygonatum cyrtonema Hua be ensured, but also the hibernation period of the seeds of polygonatum cyrtonema Hua is shortened, so that the seedbreeding process is shortened, the breeding process is simple, and the method for breeding the seeds of polygonatum cyrtonema Hua is easy to popularize and apply.

The invention discloses a selection breeding method of a new homozygous sterile line of brassica napus; according to the method, a F2 fertile plant to a transfer material is used for inbreeding; as for F3, a part of the F2 inbred single plants is hybridized with a temporary maintainer line for seed production so as to identify the F2 inbred single plant material with a homozygous sterile genotype; then the homozygous sterile line is separated and selected by the reserved single plant material; and productivity identification is performed on hybrids prepared by a restorer with seeds obtained in hybrid seed production by the selected material and the temporary maintainer line during fertility identification. The method saves a lot of labor force used in plantation and hybridization of the selected colony during selection, can predict the combining ability of the selected material at an early stage, and thus improves the breeding efficiency.

The invention discloses a breeding method of a new high-yield lodging-resistant disease-resistant wheat variety. The method comprises the following steps: hybridizing and backcrossing a dwarf, large-ear and hard-stalk lodging-resistant wheat intermediate material serving as a female parent and a disease-resistant variety Yangmai 18 serving as a male parent for one time, adding generations in a greenhouse, selecting single plants for inducing powdery mildew and resisting powdery mildew in the generation adding process, then performing molecular marker selection by combining synergistic sites QGns-YY-5B/QGbs-YY-5B of the number of grains per spike and the number of fruiting grains per spike base, planting selected strains into plant rows in a field, performing artificial induction selection on powdery mildew and gibberellic disease of advanced generations, and selecting comprehensive agronomic traits, stalk toughness lodging resistance and disease resistance. The new wheat variety bred by the method disclosed by the invention stably inherits the gibberellic disease and powdery mildew resistance of Yangmai 18, the excellent characters of good stem toughness and good lodging resistance of a non-recurrent parent are added, and the average yield is remarkably higher than that of the Yangmai 18.

The invention discloses a method for improving the moisture resistance of cabbage type rape. The method comprises the following steps of: (A), constructing a glufosinate-ammonium resistant expression vector containing vitreoscilla hemoglobin (vgb), namely, (1) obtaining a target fragment, (2) recovering a polymerase chain reaction (PCR) product, transforming and sequencing, (3) preparing transferred-deoxyribonucleic acid (T-DNA), (4) preparing Vector, (5) preparing the expression vector, and (6) preparing bacterial liquid of agrobacterium tumefaciens; (B), breeding a recovery line of the moisture-resistant cabbage type rape, namely transforming the vgb into R2 by an agrobacterium tumefaciens mediated method to obtain a T0 recovery line, and performing continuous selfing to obtain a T2 recovery line; (C), breeding a maintainer line of the moisture-resistant cabbage type rape, namely (a) transforming the vgb into 6098B by the agrobacterium mediated method to obtain a T0 maintainer line, and performing continuous selfing to obtain a T2 maintainer line, and (b) hybridizing a T1 recovery line single plant serving as a male parent with the 6098B, performing continuous backcrossing by taking the 6098B as a recurrent parent to obtain BC3, and performing selfing to obtain the T2 maintainer line; and (D), obtaining a sterile line of the moisture-resistant cabbage type rape. The method is easy and convenient to operate and obvious in effect, the cost can be reduced, and the breeding progress can be shortened.

The invention provides two microsatellite markers related to rapid growth of pseudosciaena crocea, and preparation methods thereof, and relates to molecular breeding of the pseudosciaena crocea. The two microsatellite markers related to the rapid growth of the pseudosciaena crocea are LYC0088 and LYC0143. Primer sequences of the LYC0088 are shown as SEQUENCE LISTING 1 and SEQUENCE LISTING 2; and primer sequences of the LYC0143 are shown as SEQUENCE LISTING 3 and SEQUENCE LISTING 4. The preparation methods comprise the steps of 1) screening microsatellite markers related to the rapid growth; 2) preliminary verifying the microsatellite markers related to the rapid growth; and 3) verifying again. The two microsatellite markers related to the rapid growth of the pseudosciaena crocea can be applied in selective breeding of body length, body height and body mass of the pseudosciaena crocea and in screening of the microsatellite markers related to the rapid growth of the pseudosciaena crocea.

The invention relates to the technical field of plant molecular biology, in particular to a corn waxy gene mutant as well as a molecular marker and application thereof. The invention provides a corn waxy protein mutant, and compared with a wild WAXY protein, the protein mutant comprises mutation that an amino acid sequence represented by SEQ ID NO.1 is inserted behind the 125th amino acid resulting in early termination of protein translation, so that protein translation terminates early. The invention also provides a corn waxy gene mutant, and compared with a wild waxy gene, the gene mutant comprises mutation that a nucleotide sequence shown as SEQ ID NO.4 is inserted behind the 43rd nucleotide of the 3rd exon. According to the WAXY protein mutant and the waxy gene mutant provided by the invention, the amylopectin content of corn grains can be remarkably improved, and the grains have waxy characters. The invention further provides the molecular marker of the waxy gene mutant, seedlingstage selection of waxy corn can be achieved, and the breeding period is greatly shortened.

The invention provides a method for rapidly stabilizing rice backcross introgression population trait. The method comprises the following steps: establishing selective introgression lines; screening the selective introgression lines; performing genotype identification on the selective introgression lines; analyzing the descendant donor introgression frequency and heterozygous genotype of the selective introgression lines; and breeding new variety of the selective introgression lines, performing regional tests on the selective introgression lines with obvious target performances under different ecological conditions at home and abroad, identifying the general performance of the variety, and approving or registering the bred new variety.