Molecular marker for identifying bivalent herbicide resistance gene cotton GGK2 and application of molecular marker
A technology of herbicide-resistant genes and molecular markers, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems such as limiting the application range of glyphosate
Active Publication Date: 2016-07-13
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
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Problems solved by technology
However, as a non-selective herbicide, it also has a killing effect on crops, which greatly limits the application range of glyphosate in agricultural production
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Embodiment 1
[0054] Example 1 Comparison test of PCR identification of cotton GGK2 containing bivalent herbicide-resistant gene and non-transgenic receptor material R18
[0055] (1) Test materials
[0056] (1) Plant material: bivalent herbicide-resistant genetic cotton line GGK2 (bred by Institute of Biology, Chinese Academy of Agricultural Sciences), non-transgenic receptor material R18 (obtained from Coker312), provided by Institute of Biotechnology, Chinese Academy of Agricultural Sciences ).
[0057] (2) Primers: Primers are synthesized by Shanghai Bioengineering Technology Co., Ltd.
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The invention belongs to the field of detection of genetically modified plants, and particularly discloses 2 molecular markers for identifying the specificity of bivalent herbicide resistance gene cotton strains GGK2 and derived lines. Each molecular marker consists of nucleotide sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention further discloses specific primers for amplifying the molecular markers, and purposes of the molecular markers and the specific primers. Accurate and reliable molecular markers and specific primers are provided for detection and identification of the bivalent herbicide resistance gene cotton strains GGK2 or derived materials thereof, and transgenic safety is guaranteed; then the detection method is simple, the detection speed is high, large-scale identification and screening are appropriately performed on homozygosis / heterozygote breeding materials in bivalent herbicide resistance genes in filial generations in heredity and breeding of genetically modified crops, shortening of a breeding process is facilitated, and the breeding cost is reduced.
Description
Technical field [0001] The invention belongs to the field of identification of transgenic plants, and specifically relates to a molecular marker for identifying the bivalent herbicide-resistant gene cotton GGK2, and the use of the molecular marker. Background technique [0002] Weeds seriously threaten the yield and quality of cotton. On the one hand, it competes with cotton for light, water and nutrients, and on the other hand, it serves as an intermediate host for crop diseases and insect pests, spreading diseases and insect pests. The cost of artificial weeding in cotton cultivation in my country is 3,000 yuan per hectare. By introducing herbicide-resistant genes into cotton, cotton varieties can obtain herbicide-resistant traits, and then using herbicides for weeding, not only can reduce the input of cotton farmers by more than 100 million yuan per year, but also It is of great significance to increase cotton yield and quality, reduce cotton production costs, and increase cott...
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Login to View More IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 郭三堆林敏张锐孟志刚孙豹王远孙国清陆伟张维梁成真朱涛
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI