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Method for recurrently and selectively breeding non-glutinous rice by using recessive cytoblast sterile material

A technology of recessive sterility and recurrent selection, applied in the field of crop breeding, can solve the problems of narrow genetic basis and expand the genetic basis of breeding materials, and achieve the effects of enriching the genetic basis, shortening the breeding process and time, and high combining ability

Active Publication Date: 2010-07-14
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of narrow genetic basis existing in the current rice breeding materials, and to provide a method for recurrent selection breeding of indica rice using recessive genic male sterile materials. Males can be crossed in a large number at the same time, thereby expanding the genetic basis of breeding materials; at the same time, combined with molecular marker-assisted selection to identify and eliminate sterile genes, improve breeding efficiency, and thus breed high-yield, high-quality, strong disease resistance and good adaptability. variety of

Method used

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  • Method for recurrently and selectively breeding non-glutinous rice by using recessive cytoblast sterile material
  • Method for recurrently and selectively breeding non-glutinous rice by using recessive cytoblast sterile material

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1: Breeding of three-line restorer lines using recessive genital sterile material H2S

[0043] Proceed as follows:

[0044] (1) In Wenjiang, Sichuan in 1996, the pollen-free recessive genital sterile material H2S (preservation unit: China Center for Type Culture Collection, accession number: P200905) was used as the female parent, and the restorer line Shuhui 527, Ming Hui 63, Miryang 46, Shu Hui 881, Shu Hui 885, Ming Hui 86, Ming Hui 78, Mian Hui 725, CDR22, Cheng Hui 047, Yi Hui 1577, Duoxi No. 1, Fu Hui 802, Fu Hui 838, More than 20 parents including 6078, 9308, IRBB60, Jianghui 151, Mianhui 501 and Gui 630 were used as male parents for artificial crossing, and the seeds on the sterile plants were harvested respectively to obtain F 1 substitute seeds;

[0045] (2) In the winter of 1996, F was planted in combination in Lingshui, Hainan 1 Substitute, with F 1 The plant is the female parent, and the restorer material described in (1) is used as the male par...

Embodiment 2

[0062] Example 2: Breeding of three-line maintainer lines using recessive genital sterile material H2S

[0063] (1), in Wenjiang, Sichuan in 1996, using the pollen-free Sichuan recessive genital sterile material H2S (preserved in the China Center for Type Culture Collection on September 17, 2009, the preservation number is: P200905) as the female parent, respectively. Maintained lines G46B, II-32B, Jin 23B, D62B, Zhong 9B, Zhenshan 97B, K17B, Digu B, D702B, Xianggai B, You 1B, Hongtu 31, Xinlu B, Longtepu B, IRBB23, D90B, D43B, D44B, D207B or D738B were used as the male parent for artificial hybridization, and the seeds on the sterile plants were harvested according to the combination to obtain the hybrid F 1 generation;

[0064](2) In the winter of 1996, F was planted according to the combination in Lingshui, Hainan 1 Substitute, with F 1 The generation plant was used as the female parent, and the maintainer line in (1) was used as the male parent for artificial backcrossi...

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Abstract

The invention discloses a method for recurrently and selectively breeding non-glutinous rice by using a recessive cytoblast sterile material, which mainly comprises the following steps: hybridizing a sterile strain with recessive cytoblast sterile gene ms-np serving as a female parent and 20 to 30 rice materials serving as male parents, and backcrossing one generation; then, selecting seeds on excellent sterile strains in the later generations, and equivalently mixing the seeds to form a first recurrent selective population; and repeating the recurrent selection process, selecting the excellent fertile strains to a pedigree method at any time, combining molecular marker-assisted selection, and breeding a new variety. The method has the advantages that: recessive cytoblast sterility is used for performing a large amount of hybridization so as to enrich the hereditary basis of the breeding material; new rice materials can be added at any time so as to continually improve the population; moreover, the method avoids manual castration and pollination so as to save a large amount of manpower; and by utilizing a DNA molecular marker for assisting in selection, the method shortens the breeding progress and time, and reduces the breeding cost.

Description

technical field [0001] The invention belongs to crop breeding methods, and in particular relates to a method for recurrent selective breeding of indica rice using recessive nuclear sterile materials. Background technique [0002] Rice is a self-pollinated crop. For a long time, the improvement of rice varieties has mainly relied on hybrid breeding methods. However, due to the limitations of artificial castration and hybridization techniques, usually only a small number of parents can be used for hybridization. Difficult to breed breakthrough breeds. The main agronomic traits of rice are mostly quantitative traits controlled by micro-effect multi-genes. Recurrent selection breeding method can break the linkage of unfavorable genes and increase the accumulation of good genes. Therefore, it is an important means to expand the genetic base and breed breakthrough varieties. Restrictions such as difficulty in emasculation limit the application of recurrent selective breeding meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02C12Q1/68C12N15/11
Inventor 李仕贵王玉平高克铭马炳田钦鹏吴先军
Owner SICHUAN AGRI UNIV
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