Method for breeding maize inbred line

A technology of inbred lines and corn, applied in the direction of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding cycle and narrow genetic base, and achieve the effect of short breeding cycle and rich genetic base

Inactive Publication Date: 2018-11-20
JILIN ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the breeding of maize inbred lines is mainly the application of the two-ring line breeding technology, that is, the combination of two inbred lines into single-cross varieties is used as the basic material for line selection, and a stable inbred line is obtained after multiple self-crosses. This method has a long breeding cycle and a narrow genetic basis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for breeding corn inbred lines, characterized in that it comprises the steps of:

[0015] 1) Select 4 inbred lines of maize with outstanding target traits in the same heterosis group to cross pairwise 6 single-cross maize A×B, A×C, A×D, B×C, B×D, C× D;

[0016] 2) Take 200 grains of each of the six single-cross varieties A×B, A×C, A×D, B×C, B×D, and C×D and plant them in the same isolated area, and plant one row for each single-cross variety. Free loose powder, each row is mixed and harvested for threshing to obtain E1, E2, E3, E4, E5, E6;

[0017] 3) Take 500 grains of corn E1, E2, E3, E4, E5, and E6 that are mixed and harvested in the 6 rows in step 2), plant them in the same isolation area, loose powder freely, select several individual plants that meet the target requirements, and harvest separately , threshing separately to obtain F1, F2...Fn;

[0018] 4) In step 3), the maize F1, F2...Fn obtained after harvesting and threshing each selected individual ...

Embodiment 2

[0020] A method for breeding corn inbred lines, characterized in that it comprises the steps of:

[0021] 1) Select 4 inbred lines of maize with outstanding target traits in the same heterosis group to cross pairwise 6 single-cross maize A×B, A×C, A×D, B×C, B×D, C× D;

[0022] 2) Take 200 grains of each of the six single-cross varieties A×B, A×C, A×D, B×C, B×D, and C×D and plant them in the same isolated area, and plant one row for each single-cross variety. Free loose powder, each row is mixed and harvested for threshing to obtain E1, E2, E3, E4, E5, E6;

[0023] 3) Take 400 grains of corn E1, E2, E3, E4, E5, and E6 that are mixed and harvested in the 6 rows in step 2), plant them in the same isolation area, loose powder freely, select several individual plants that meet the target requirements, and harvest separately , threshing separately to obtain F1, F2...Fn;

[0024] 4) In step 3), the maize F1, F2...Fn obtained after harvesting and threshing each selected individual ...

Embodiment 3

[0026] 1) Select 4 inbred lines of maize with outstanding target traits in the same heterosis group to cross pairwise 6 single-cross maize A×B, A×C, A×D, B×C, B×D, C× D;

[0027] 2) Take 100 grains of each of the six single-cross varieties A×B, A×C, A×D, B×C, B×D, and C×D and plant them in the same isolation area, and plant one row for each single-cross variety. Free loose powder, each row is mixed and harvested for threshing to obtain E1, E2, E3, E4, E5, E6;

[0028] 3) Mix and harvest 300 grains of corn E1, E2, E3, E4, E5, and E6 in the 6 rows of step 2), plant them in the same isolation area, loose powder freely, select several individual plants that meet the target requirements, and harvest separately , threshing separately to obtain F1, F2...Fn;

[0029] 4) In step 3), the maize F1, F2...Fn obtained after harvesting and threshing each selected individual plant separately are each planted into a population, and it is used as the female parent, and the corn parthenogenesi...

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Abstract

The invention relates to a method for breeding a maize inbred line, which is characterized by comprising the following steps: selecting four inbred lines with prominent target traits of a same heterotic group, performing pairwise hybridization to match six single cross hybrids, planting the six single cross hybrids in the same isolation area, planting one row per single cross hybrid, allowing freepollening, performing mixed harvest and threshing in each row, mixing in equal amount in the next season, planting in the same isolation zone, allowing free pollening, selecting several single plantsthat meet target requirements, then planting each of the selected single plants into a single group in the next season, taking the group as a female parent, taking a maize parthenogenetic induction line as male parent for induction, and breeding the inbred line by doubling. The method has the advantages that: for deficiencies of long cycle and narrow genetic basis of inbred lines of a second cycle line, the six single cross hybrids generated by pairwise hybridization of four inbred lines of the same group are utilized, pollening is performed by two times of isolation, a homozygous inbred lineis bred by selecting the single plants and utilizing a haploid breeding technique, only four generations are required, a breeding cycle is short, and the genetic basis is rich.

Description

technical field [0001] The invention belongs to the technical field of new agricultural species breeding, and relates to a method for breeding corn inbred lines. Background technique [0002] At present, the breeding of maize inbred lines is mainly the application of the two-ring line breeding technology, that is, the combination of two inbred lines into single-cross varieties is used as the basic material for line selection, and a stable inbred line is obtained after multiple self-crosses. This method has a long breeding cycle and a narrow genetic basis. Contents of the invention [0003] The object of the present invention is to provide a method for breeding maize inbred lines to solve the problems raised in the above-mentioned background technology. [0004] In order to solve the above-mentioned technical problems, the technical solution provided by the invention is: a method for breeding corn inbred lines, comprising the following steps: [0005] 1) Select 4 maize in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02
CPCA01H1/02A01H1/04
Inventor 焦仁海刘兴二代秀云徐艳荣仲义苏义臣侯宗运
Owner JILIN ACAD OF AGRI SCI
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