33results about How to "Fast breeding" patented technology

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The invention belongs to a cultivation method for the seabuckthorn fruit asexual propagation, especially relating to a method for breeding the seabuckthorn fruit with no lunge and big fruit, comprising: employing the sprig growing in the parent tree of 2-3 years old of Liaofu 1 and Liaofu 2 which grows no lunge and big fruit as cutting branch for the asexual propagation, building seed bed and irrigating facilities, proceeding cuttage and management after the cuttage, etc. The seabuckthorn fruit with no lunge and big fruit is the strain with high quality among the seabuckthorn fruit strains, the breeding speed of seedling is high by applying sprig for cuttage, the breeding speed can be increased by employing the water from square pool in the spray irrigation with the average plant height 11 cm higher, ground diameter 0.09 cm wider than that of employing water from deep well in the same year, the plant percent is 95%, 15% higher than former, the survival rater of nursery transplant is 80%, 8% higher than former.

The invention discloses a damask rose twig cutting seedling-raising method which includes the steps: S1 performing laying and disinfecting of seedling beds; S2 performing preparation and disinfection of culture media and layout of plugs; S3 cutting semi-lignified twigs without diseases and insect pests on stock plants, performing water spraying and moisturizing on the cut semi-lignified twigs, cutting cuttings at shade places, and performing disinfection on the cut cuttings; S4 plunging deep holes in the culture media, treating the disinfected cuttings by the aid of rooting agents, performing cutting on the treated cuttings, lightly pushing sealing openings with fingers after cutting, and enabling the culture media and the cuttings to be closely connected; S5 performing reasonable atomizing and light supplement management after cutting. 2-3 bud joints are reserved on each cutting, the length of each cutting is about 8cm-10cm, 4 leaflets of the upper portion of each cutting are reserved, the position 5 millimeters away from the uppermost bud joint of each cutting is horizontally cut, the position 3-5 millimeters away from lowermost bud joint of each cutting is cut into a 45-degree inclined plane, and the cutting depth does not exceed 3cm. The method is rapid in rooting, high in transplanting survival rate, simple and easy to operate, and a seedling-raising period can be greatly shortened.

The invention relates to a treatment method for inducing maize parthenogenesis with colchicine. The method is characterized in that when female ear filaments are completely exposed, a paper bag is removed; the upper-portion of a flower bud is cut transversely from a female ear flower bud at a place from the top and close to the female ear young ear downward; a part of filaments is cut at the section with a curved graver, such that a cavity with a depth of 5-15mm is formed; an inducing agent which is colchicine treatment liquid is injected into the cavity with a pipette or a syringe, such that the cavity is filled with the liquid and the entire cut surface is wetted by the liquid; and the female ear is sleeved with the paper bag. During the treatment process, no pollen contamination prevention measure such as sealing, blocking, spatial and temporal isolation, and the like is needed. The method has the following advantages: harm to human health caused by treatment liquid dropping, spilling and scratching are avoided; a hard-to-solve pollen contamination problem is thoroughly solved; all filaments can contact the treatment liquid, and treatment liquid retention time is long, such that an induction rate is improved; the treatment liquid is saved by approximately 50%; and compared to a conventional inbred line breeding method, a breeding period is shortened by more than 5 generations.

The invention discloses a needle mushroom fruiting and breeding method. By adopting the method to breed needle mushroom strains, the invention has the advantages of rapid breeding speed, short period, less energy consumption, high efficiency, stable inheritance, high controllability and the like.

The invention provides a treating method for chemically inducing parthenogenesis of corn. The method is characterized by comprising the following steps: adding roll paper into an inductive agent and carrying out stirring so as to prepare paper paste; sleeving a female ear with a paper bag before filament emergence; picking off the paper bag when filaments come out and transversely cutting off a basal part at the top of the bud of the female ear with a blade so as to form a circular cross section which has filaments at the center and husk leaves at the periphery; picking up the paper paste with dressing pliers, placing the paper paste on the cross section and allowing the paper paste to closely contact with the cross section and fill in space formed after the cutting; restoring pulled-open husk leaves to original positions so as to allow the paper paste to be packed in the husk leaves; and finally, sleeving the treated ear with the paper bag again. The invention has the following beneficial effects: the problem of pollen pollution difficult to overcome in a conventional chemical-induction treatment method is thoroughly overcome; threat to human health is avoided; induction operation is carried out under completely open conditions; an induction rate is improved; loss of the liquid of the inductive agent is small; and compared with a conventional selfing line breeding method, the breeding period in the method provided by the invention is shortened by more than 5 generations.

A process for preparing the hybrid seeds of rape and the vegetables in the mustard family by using the sterile cytoplasm of charlock includes such steps as separating the protoplast from leaf pulp, purifying it, pre-treating, fusing, culturing, discriminating the hybrid, reproduction, selectively culturing the male sterility recovery line of charlock cytoplasm, transferring the male sterility and recovery characters from the charlock cytoplasm to rape or the vegetables in mustard family, and hybridizing between the charlock cytoplasm male sterility line of rape or the said vegetables and the male fetile rape or the said vegetables.

The invention discloses a cutting breeding method of Passiflora edulis Sims. The method comprises the following steps: choosing land; preparing a cutting medium; determining a proper cutting time; choosing scions; scissoring the scions; coating the scions with a pulp; performing cuttage; performing watering; controlling the temperature of a greenhouse; controlling the humidity of the greenhouse; and performing fertilization. The method provided by the invention has advantages of fast breeding speed, high survival rate, large breeding quantity, and less female parent viruses. According to the invention, the survival rate is increased by 15-25% compared with that of the prior art, and not only is the seedling breeding cost reduced, but also the Passiflora edulis Sims breeding progress is accelerated. The method provided by the invention provides a scientific and technological base for the growth of Passiflora edulis Sims.

The invention discloses a method for cultivating bulb tissues of alliums. The method comprises the following steps: selecting the underground bulbs of the alliums as explants and performing disinfection treatment; inoculating the explants to an MS culture medium containing 2,4-dichlorphenoxyacetic acid with the concentration of 0.1 to 0.5 mg/L, thidiazuron with the concentration of 0.1 to 1.0 mg/Land vitamin B6 with the concentration of 0.5 to 1.5 mg/L, and sequentially performing heat shock treatment, lucifugal cultivation and illumination cultivation; and transplanting the explants to a White culture medium containing indolebutyric acid with the concentration of 0.1 to 0.5 mg/L, thidiazuron with the concentration of 0.1 to 1.0 mg/L and vitamin B1 with the concentration of 8 to 10 mg/L and performing rooting cultivation. Different culture mediums are used according to the nutritional requirements in different cultivation stages, the corresponding cultivation method is assisted, and synergistic effect is achieved, so that the adventitious bud induction efficiency and rooting rate are effectively improved, the proliferation rate is increased, the proliferation time is short and thebreeding speed is high.

The invention relates to the field of flower planting, in particular to a seedling breeding method of Rhododendron fortunei. According to the method, seedlings of the Rhododendron fortune are bred with a tissue culture technique, so that the breeding speed is high, obtained seedlings are improved varieties, the plant shape is attractive, leaf color is invisible green and flowers are flourishing after the seedlings grow up, the flowering stage is 15-20 days longer than that of existing varieties, and ornamental value is improved; due to addition of Chinese herbal medicine extract to a culture medium, the disease resistance of Rhododendron fortunei plants is enhanced, and the occurrence rate of pest and disease damage of the Rhododendron fortunei is reduced.

The invention discloses a method for rapid breeding of the sterile line of Shaanxi 5201. The breeding method includes the following steps: the implantation effect of the self-incompatible line 5201 is the best, and can achieve 100% infertility. Fertility rate and 100% sterility; in the process of male sterile line transfer of Shaanxi 5201, by selecting a single plant similar to the maintainer line and with normal flower bud development from the second-generation segregation population to continue the transfer, it can be quickly obtained Purpose of transfer. At the same time, selecting a single plant with a high affinity index for sterile line transfer will greatly reduce the cost of breeding parents and increase the yield of seed production.

The invention relates to a method for adopting oryza eichingeri young embryos for breeding regeneration plants, and belongs to the technical field of plant tissue culture. The method comprises the steps of explant selection, explant sterilization, callus induction and enrichment culture, callus differentiation culture, differentiated seedling rooting culture, seedling hardening and transplanting.By applying different plant hormone combinations and culture condition combinations, the oryza eichingeri callus is successfully induced, the callus is transferred to an enrichment medium for enrichment culture, the callus quality is improved, and the callus number is increased. The method has the advantages that oryza eichingeri regeneration plants can be efficiently obtained, the breeding speedis high, the period is short, the problem that oryza eichingeri resources are not sufficient is solved, and the method is of great significance in protecting endangered oryza eichingeri resources.