Treatment method for inducing maize parthenogenesis with colchicine

A technology of colchicine and parthenogenesis, which is applied in the field of corn breeding, can solve the problems of many false seeds and high pollen pollution rate, and achieves the effects of simple and easy operation, simple procedure and solving the problem of pollen pollution.

Active Publication Date: 2015-05-13
SHENYANG TEYIJIA CORN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is necessary to take advantage of the advantages of the high induction rate of colchicine, and to solve the safety problems in the existing methods in the operation method; (2) to solve the common problems of high pollen pollution rate and many false seeds in the existing methods of chemical induction ; (3) Simplify the processing procedure, make the operation simple and easy for large-scale application; (4) Simultaneously improve the induction rate and reduce the dosage of the drug

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of inducer

[0049] Colchicine (COL) was diluted with water to a concentration of 0.1% inducer.

[0050] (2) Select processing test material

[0051] The object of treatment is the maize hybrid "Liaodan 565".

[0052] (3) Ear treatment before induction

[0053] Ears are bagged before silking. Take off the paper bag after the filaments are fully spit out, and cut off the upper flower buds horizontally at the position where the ear buds are close to the young ears of the ear from the top, and cut off a section of filaments on the section with an arc-shaped carving knife, and the depth of cutting is 5- 15mm, do not cut out the surrounding bracts, forming a cavity with a depth of 5-15mm.

[0054] (4) Apply inducer

[0055] Inject the inducer into the cavity with a pipette to fill and cover or wet the entire cut surface. Finally put back into the paper bag. 60 panicles were processed.

[0056] Operating conditions: The operating process is carried out...

Embodiment 2

[0062] (1) Preparation of inducer

[0063] Colchicine and dimethyl sulfoxide (DMSO) sold in the market are selected, and the inducer is compounded according to the following weight ratio: 0.2% colchicine, 2% dimethyl sulfoxide, and the balance is water.

[0064] (2) Select processing test material

[0065] The object of treatment is sweet corn hybrid "Shentian 6".

[0066] (3) Ear treatment before induction

[0067] Ears are strictly bagged before silking. When the filaments are fully spit out, take off the paper bag, cut off the upper flower bud horizontally at the position where the ear bud is close to the young ear of the ear from the top, and then cut off a section of filament on the section with an arc-shaped carving knife. The cutting depth is 5-5. 15mm, do not cut out the surrounding bracts, forming a cavity with a depth of 5-15mm.

[0068] (4) Apply inducer

[0069] Inject the inducer into the cavity with a pipette to fill and cover or wet the entire cut surface. ...

Embodiment 3

[0076] (1) Preparation of inducer

[0077] Select colchicine, dimethyl sulfoxide and green fresh element (MH) sold on the market for use, carry out compound inducer according to the following weight ratio: colchicine 0.2%, dimethyl sulfoxide 2%, 40mg·kg -1 cyanin, the balance is water.

[0078] (2) Select processing test material

[0079] The object of treatment is the maize hybrid "Xianyu 335".

[0080] (3) Ear treatment before induction

[0081] Strictly bag the ears before spinning. When the filaments are completely spit out, take off the paper bag, and cut off the upper flower bud horizontally at the position where the ear bud is close to the ear young ear from the top to form a circular cross section, and then dig out a section of the filament on the section with an arc-shaped carving knife. The depth of cutting is 5-15 mm, and the surrounding bracts are not cut to form a cavity with a depth of 5-15 mm.

[0082] (4) Apply inducer

[0083] Inject the inducer into the...

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PUM

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Abstract

The invention relates to a treatment method for inducing maize parthenogenesis with colchicine. The method is characterized in that when female ear filaments are completely exposed, a paper bag is removed; the upper-portion of a flower bud is cut transversely from a female ear flower bud at a place from the top and close to the female ear young ear downward; a part of filaments is cut at the section with a curved graver, such that a cavity with a depth of 5-15mm is formed; an inducing agent which is colchicine treatment liquid is injected into the cavity with a pipette or a syringe, such that the cavity is filled with the liquid and the entire cut surface is wetted by the liquid; and the female ear is sleeved with the paper bag. During the treatment process, no pollen contamination prevention measure such as sealing, blocking, spatial and temporal isolation, and the like is needed. The method has the following advantages: harm to human health caused by treatment liquid dropping, spilling and scratching are avoided; a hard-to-solve pollen contamination problem is thoroughly solved; all filaments can contact the treatment liquid, and treatment liquid retention time is long, such that an induction rate is improved; the treatment liquid is saved by approximately 50%; and compared to a conventional inbred line breeding method, a breeding period is shortened by more than 5 generations.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, in particular to a method for breeding corn inbred lines. Background technique [0002] The conventional method of breeding maize inbred lines requires at least 7 generations of continuous selfing to breed an inbred line, and even more than 10 generations for materials with complex genetic backgrounds. In order to speed up the breeding speed of inbred lines, shorten the breeding cycle and improve the purity, the methods commonly used at home and abroad mainly include: [0003] 1. Colchicine induction method [0004] At the silking stage of maize, colchicine was used as a chromosome doubling agent to treat the filaments so that they could directly produce homozygous diploid seeds through parthenogenesis without double fertilization. [0005] Colchicine is a commonly used chromosome doubling treatment agent, and its outstanding advantage is its high induction rate, so it is widely used in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 史振声张喜华
Owner SHENYANG TEYIJIA CORN TECH
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