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Method for cultivating bulb tissues of alliums

A technology for allium plants and tissue culture, applied in the field of plant tissue culture, can solve the problems of low induction efficiency and low proliferation rate of Allium plants, achieve accelerated callus and culture growth, high proliferation rate, and improve induction efficiency Effect

Inactive Publication Date: 2018-05-15
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome the low induction efficiency and low multiplication rate defects of using plant tissue culture technology to cultivate Allium plants in the prior art, thereby providing a high-efficiency Allium plant bulb tissue culture method

Method used

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  • Method for cultivating bulb tissues of alliums
  • Method for cultivating bulb tissues of alliums
  • Method for cultivating bulb tissues of alliums

Examples

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preparation example Construction

[0025] The preparation method of MS culture medium used in the present invention is as follows:

[0026] 1) Mother liquor configuration

[0027] Contrast Table 1-4, weigh each component respectively.

[0028] Table 1 MS macroelement mother liquor formula

[0029]

[0030] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0031] Table 2 MS trace element mother liquor formula

[0032]

[0033] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0034] Table 3 Formula of MS inositol mother liquor

[0035]

[0036] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0037] Table 4 Formula of MS iron salt mother liquor

[0038]

[0039] After weighing, dissolve thoroughly with a small amount of distilled water and mix, and finally make up to 1L.

[0040] 2) MS medium configuration ...

Embodiment 1

[0058] Present embodiment provides a kind of onion bulb tissue culture method, comprises the steps:

[0059] (1) Select the underground bulb of green onion as an explant, remove the outer two layers of bulbs, rinse with tap water for 15 minutes, rinse with alcohol with a volume percentage concentration of 75% for 15 minutes, and then soak in 0.1% HgCl 2 After being disinfected in the solution for 10 minutes, take it out and rinse it with sterile water 5 times for 30 seconds each time, and then remove the surface moisture with sterile paper;

[0060] (2) Inoculate the explants after the disinfection treatment in the MS medium with a pH of 6.2, and the MS medium used contains 2,4-dichlorophenoxyacetic acid with a concentration of 0.3mg / L and a concentration of 0.5mg / L thiadizuron and vitamin B at a concentration of 1.0mg / L 6 , and 3% sucrose, cultured in the dark at 35°C for 4 days, then cultured in the dark at 25°C for 15 days, and then cultivated for 15 days under the conditi...

Embodiment 2

[0064] Present embodiment provides a kind of leek bulb tissue culture method, comprises the steps:

[0065] (1) Select the underground bulbs of Chinese chives as explants, remove the outer layer of bulbs, rinse with tap water for 10 minutes, rinse with alcohol with a concentration of 70% by volume for 20 minutes, and then soak in 0.1% HgCl 2 After being sterilized in the solution for 8 minutes, take it out and rinse it with sterile water 4 times for 40 seconds each time, and then remove the surface moisture with sterile paper;

[0066] (2) Inoculate the explants after the disinfection treatment in the MS medium with a pH of 6.5, and the MS medium used contains 2,4-dichlorophenoxyacetic acid with a concentration of 0.5mg / L and a concentration of 0.1mg / L thiadizuron and vitamin B at a concentration of 1.5mg / L 6 , and 2% sucrose, cultured at 30°C in the dark for 5 days, then at 28°C in the dark for 10 days, then under the condition of light intensity of 1500Lx and temperature of...

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Abstract

The invention discloses a method for cultivating bulb tissues of alliums. The method comprises the following steps: selecting the underground bulbs of the alliums as explants and performing disinfection treatment; inoculating the explants to an MS culture medium containing 2,4-dichlorphenoxyacetic acid with the concentration of 0.1 to 0.5 mg / L, thidiazuron with the concentration of 0.1 to 1.0 mg / Land vitamin B6 with the concentration of 0.5 to 1.5 mg / L, and sequentially performing heat shock treatment, lucifugal cultivation and illumination cultivation; and transplanting the explants to a White culture medium containing indolebutyric acid with the concentration of 0.1 to 0.5 mg / L, thidiazuron with the concentration of 0.1 to 1.0 mg / L and vitamin B1 with the concentration of 8 to 10 mg / L and performing rooting cultivation. Different culture mediums are used according to the nutritional requirements in different cultivation stages, the corresponding cultivation method is assisted, and synergistic effect is achieved, so that the adventitious bud induction efficiency and rooting rate are effectively improved, the proliferation rate is increased, the proliferation time is short and thebreeding speed is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for culturing bulb tissue of Allium plants. Background technique [0002] Allium is an important vegetable and condiment in my country, and it is also widely used in Japan, South Korea and Southeast Asian countries. The traditional propagation method is mostly by dividing bulbs or seeds, but it takes 3-4 years to produce bulbs or seeds, sowing production is limited by season or climate, and the number of reproduction is limited, and allium plants are mostly cross-pollinated crops, multi-generational The self-breeding recession is serious, and the reproductive quality cannot be guaranteed. In addition, some plants of the Allium genus are underground for a long time and are susceptible to virus infection. Once the bulbs or seeds are infected with the virus, they cannot be eradicated, resulting in a significant drop in the quality of the reproducti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨平张边江王立科唐宁陈全战
Owner NANJING XIAOZHUANG UNIV
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