154results about How to "Reduce the cost of seed production" patented technology

The invention provides a propagation method for a rice normcore sterile line. The propagation method comprises the following steps of establishing an expression vector containing a color marker gene C, a normcore sterile fertility recovery gene S and a pollen inactivation gene K; converting the expression vector into rice normcore sterile mutant strains to obtain T0 generation transgenic plants; selecting fertile plants, having the three genes of the color marker gene C, the normcore sterile fertility recovery gene S and the pollen inactivation gene K, from the T0 generation transgenic plants; performing self-fertilized propagation on the fertile plants, and separating out engineering maintainer line seeds and normcore sterile line seeds from the seeds obtained from self-fertilized propagation offspring; and taking the normcore sterile line seeds as a female parent while taking the engineering maintainer line seeds as a male parent, performing hybridization on the female parent and the male parent, and obtaining seeds from female parent plants, namely normcore sterile line seeds without transgenic components. The propagation method has the advantages of high occupation proportion of the sterile line seeds, low production cost and the like.

The invention provides a method of crossbreed seed producing which performs rhizocarpous regeneration by use of the cotton sterile male line and restorer line, and the steps are that: a. first year, in the region where the cotton is able to live through the winter, the cotton male sterile line and the restorer line are respectively planted, the restorer line is used as the male parent to crossbreed with the sterile line to produce the F1 crossbreed cotton seeds so as to being used for the large-area production; b. after gaining the seeds, the sterile line and the restorer line plant live through the winter in natural environment; c. next year, the sterile line and the restorer line plant through the winter are pruned and trained, subsequently, the restorer line is used as the male parent to crossbreed with the sterile line during the flowering period, again the F1 crossbreed cotton seeds are produced so as to being used for the large-area production; d. the two steps of b and c are repeated for 1 to 3 times. The invention promises one year planting and continuously gaining the crossbreed seeds for many years, the invention provides labor and time saving for crossbreed seed production with low cost; moreover, the advantages of the crossbreed can be kept for a long time and the planting output and the quality of the crossbreed cotton can be assured, which is convenient for the commercialized application and has extremely important significance in the popularization of the utilization technology of the cotton crossbreed advantages.

The invention discloses an Agaricus Bisporus liquid spawn and a preparation method thereof. The Agaricus Bisporus liquid spawn is produced by means of three-level propagation through slant and shake-flask media and a fermenter medium. The slant and shake-flask media comprise components including 100-200g of potato, 50-150g of cow dung, 10-30g of glucose, 1-2.5g of mono potassium phosphate, 0.5-2g of magnesium sulfate and 1-4g of peptone; the fermenter medium of every 50 liters of water is composed of 2-4kg of potato, 1-3kg of cow dung, 0.5-1kg of bran, 0.2-0.4 kg of corn powder, 0.5-1kg of sucrose, 30-60g of mono potassium phosphate, 20-30g of magnesium sulfate, 120-150 mg of vitamin B and 40-50g of defoamer. By application of the Agaricus Bisporus liquid spawn and the preparation method thereof, the problem that mycelia are prone to aging and degradation during growth is solved; the liquid spawn is of globular mycelia with uniform age, high activity and fast running; a high-running spawn center is formed after the liquid spawn is inoculated to solid media, with uniform fruiting achieved; the preparation cost is only one-tenth of that of solid spawn; the growth cycle of fermenter spawn is 7-9 days, and thus spawns on a large scale can be cultured within a short time.

The breeding method for Yebai cytoplasmic sterile line includes adopting the filial generation of wild wild kenaf variety UG93 or its filial generation as female parent and cultivated kenaf variet as male parent for saturated backcrossing to selecting Yebai type regular cytoplasmic male sterile line with character similar to that of the male parent; and backcrossing to obtain male parent male sterile line. The selected male sterile line may be further naturally crossed with other kenaf variety to obtain cross combination with powerful heterotic vigor.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The invention provides a rapid reproduction method of konjac. In the method, rhizome without flower buds in those years or annual-triennial konjac is induced for flowering by induction of gibberellin (GA3) on the flower buds of the konjac, thus lowering cost of obtaining female parent; and meanwhile the flowering season of male parent is regulated and controlled to meet the flowering season of the induced female parent for creating the chance of pollination by inhibition of low temperature on growth of the flower buds of the konjac. Compared with a natural condition, the method can help rapidly increase the female parent material in quantity with low cost; the male parent can be successively cultivated after low-temperature storage, thus ensuring that the male parent meets the flowering season of the induced female parent; and a great quantity of low-cost konjac seeds can be obtained by hybridization of a small amount of the male parent and a great amount of the induced female parent.

The invention relates to a method for producing seeds of hybrid rice. The method has the advantages of high production efficiency, low cost and capability of controlling the growth period of male parents. The method for producing the seeds of the hybrid rice comprises the following steps of: 1) selecting a region and time for seed production; 2) raising seedlings by using the male parents: putting nutritional soil into each seedling raising tray, soaking seeds to accelerate germination of endosperm exposed male parent seeds, placing the seedling raising trays filled with the nutritional soil and the male parent seeds for 48 hours, then transferring to a seedling raising field, and spreading single trays; 3) transplanting the male parent seedlings: transplanting the male parent seedlings obtained in the step 2 to a seed production field by using a rice transplanter; 4) directly seeding and managing female parents: when the male and female parent seeding time difference is within 2 weeks, directly seeding female parent seeds to the seed production field where the male parent seedlings are transplanted; when the male and female parent seeding time difference is more than 2 weeks, conventionally accelerating germination of the female parent seeds and then seeding the female parent seeds to the seed production field where the male parent seedlings are transplanted; 5) monitoring differentiation of young ears and regulating the flowering period; 6) managing the heading and flowering periods; and 7) harvesting.

The invention relates to a hybrid round eggplant seed production method and belongs to the technical field of vegetable seed breeding. The method comprises the steps of sowing, planting, pruning, pollinating and harvesting and is characterized in that: during the pruning, four-branch pruning is performed; door eggplants and opposite eggplants are removed; and pollination and fruits reservation are performed after buds of bucket-shaped eggplants are matured. The method has the advantages of simpleness, easy fruit setting, unified nodal region, nutrition insurance, uniform fruits, low seed production cost, high plant stress resistance, low fruit morbidity, many seeds in a single plant, high seed production yield and the like.

The invention discloses a breeding method for black and white sweet glutinous hybrid maize. The breeding method for black and white sweet glutinous hybrid maize comprises the steps of selecting ultra-sweet maize, glutinous maize, a common high yield multi-resistance maize-inbred line and the like from different regions to be equivalently mixed, openly pollinating the maize, establishing a basic group A, selecting black glutinous maize germplasm from different regions to be equivalently mixed, openly pollinating the maize, and establishing a basic group B; then carrying out recurrent selection to the properties of seeds of the maize to respectively form a white sweet glutinous maize recurrent selection group A and a black sweet glutinous maize recurrent selection group B, obtaining a serious inbred line through multi-generation inbreeding, and screening a white sweet glutinous maize inbred line 4587 and a black sweet glutinous maize inbred line 3303 through combination ability measurement and cross combination quality identification to obtain 4587 X black 3303 hybrid. The breeding method can ensure both the glutinous and sweet genes of a parent inbred line and the hybrid of the parent inbred line, and also ensure the appearance quality, the favorable edible quality, the stability and the consistency of the hybrid. Moreover, the seeds of the maize are black and white.

The invention discloses a method for producing edible sunflower hybrid seeds. The method comprises the following steps of: (1) culturing parents a, namely planting an edible sunflower variety a which has high combining ability and good economic characteristic in a production site; and through 5 to 8 generations of sister hybridization, selecting strains which have strong adaptability and high purity without degeneration after the sister hybridization as the parents a; (2) culturing parents b, namely planting an edible sunflower variety b which has the same flowering phase, maturation phase and strain height as those of the parents a, and has disease resistance and seed size complementary for those of the parents a; and after 5 to 8 generations of self-copulation, selecting the strains which have the high combining ability as the parents b; and (3) producing hybrid seeds, namely planting the parents a and the parents b at the same time; and mutually pollinating the parents a and the parents b in the flowering phase in order to culture the hybrid seeds. The method for producing the edible sunflower hybrid seeds has the characteristics of simple processes, low cost, randomly selected hybrid combination and strong combination advantages. The hybrid seeds have the advantages of disease resistance, high yield, good quality, strong growth potential and the like.

The invention discloses a method for cultivating grain-saving black-feathered green-shell hens, which specifically comprises the cultivation of high-yield black-feathered green-shell hens and the cultivation of grain-saving black-feathered green-shell hens. The present invention combines the knowledge of genetics to give full play to the genetic effects of dw genetic linkage, black feather and green shell genes, and uses the cultivated high-yielding green-shell hens as the female parent and the green-footed dwarf laying hens as the male parent to cultivate a grain-saving Type black-feathered green-shell layer hens, commercial chickens have good appearance, less material consumption, high egg production rate, and 100% green shell rate.

The invention discloses a breeding method of a yellow bud marker photosensitive male sterile line. The breeding method provided by the invention comprises the following step: performing autocopuation on the yellow bud marker photosensitive male sterile line under the condition of being within 11 hours and 12 hours and 20 minutes so as to get seeds of the yellow bud marker photosensitive male sterile line. The yellow bud marker photosensitive male sterile line is the sterile line, is also a maintainer line of the sterile line, and further has the characteristics of a wide range of restoration sources, free assembly, simple seed production procedure and low seed production cost. The yellow bud marker photosensitive male sterile line comprises a yellow bud marker, hybrid seeds can be effectively removed by screening markers during the seedling stage and photosensitive sterile strains can be further determined. The yellow bud marker photosensitive male sterile line can provide seed resources for breeding of the new photosensitive sterile lines, greatly reduce the cost of artificial emasculation, assemble a large number of hybrid combinations, breed the hybrid seeds and accelerate the breeding process.

The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN250, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN250 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN250 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.

The invention relates to the field of genetic breeding of domestic fowls and particularly relates to a selective breeding method for high-uniformity slaughter type high-quality spotted-brown chickens.The method comprises the steps: establishing three specialized lines, wherein a line A is a rapid-big spotted-brown chicken assorted terminal paternal line, is a normal type spotted chicken and is medium or relatively high in growth speed; a line B is a DW-linked-gene spotted-brown chicken pure line, is a dwarf type spotted chicken, is medium or relatively low in growth speed and contains an ultrarapid-big AA chicken blood line; and a line C is a normal type brown chicken and is medium or relatively high in growth speed; producing a parent-generation BC line by taking a cultured line C as a first female parent and taking a cultured line B as a first male parent; and producing commodity chickens ABC by taking a cultured line AA as a terminal male parent and taking selected and reserved hens of the parent-generation BC line as a terminal female parent. Through the method, the high-quality chickens, which are suitable for being subjected to factory slaughter processing and are obvious inslaughter property advantages, can be cultured; and through a mode of matching of three lines, a new variety of high-uniformity slaughter type high-quality cold-fresh chickens, which are stable in genetics, meet requirements of a live chicken market and can be directly applied to product, is bred.

The invention discloses a feather color automatic sexing hen mating strain and a hen mating strain four-strain-three-purpose mating using method, and belongs to the technical field of poultry breed production. According to the hen mating strain, a dwarf hen strain with the white eggshell and the yellow appearance feather color serves as a male parent, a normal brown laying hen strain with the white appearance feather color and the wing tips, the tail, the shoulders and the neck with the black feather color serves as a female parent, a pink shell soil specie laying hen mating strain is obtained through hybridization, the appearance feather color of a cock is mainly white, the feather color of the hen is jute feather, and automatic sexing can be carried out according to the feather colors. According to the using method, the white eggshell hen strain, the brown shell hen strain, the middle-speed yellow feather hen strain and the slow-speed yellow feather hen strain are used for breed production, a pink shell soil specie laying hen, a middle-speed type broiler hen and a low-speed type broiler hen are produced, organic fusion of the three kinds of hens is achieved, the high-quality hen four-strain-three-purpose breed production is achieved, the product diversification is achieved, the product structure can be flexibly adjusted according conditions, the risk resisting capacity is strengthened, and the economic benefit is guaranteed.

The invention relates to a breeding method of crops, and particularly to a breeding method of broccolini, belonging to the technical field of plant breeding. The breeding method has the advantages that 1. the male sterility line belongs to cytoplasmic male sterility and maternal inheritance, and is stable in sterility, the sterile plant rate and the sterility are 100 percent, nectary is developed, more nectars grow, stigma is normal, flowering and fruiting characters are good, and a seedling does not yellow under the condition of low temperature and can not deteriorate after multi-generation backcross, transform is easy, growth is vigorous, and combining ability is strong; 2. a transformation early generation sterile line is stronger in growth vigor in comparison with a maintainer line and has the advantage of interspecies cross; 3. a later producing shoot of the transformation early generation sterile line is stronger than that of the maintainer line, and thus the transformation early generation sterile line material is early planted by 5 days in comparison with a backcross male parent; 4. F1 is prepared by using the sterile line, the seed yield is high, the seed producing cost is low and the labor is saved; and 5. a new variety prepared by using the sterile line is strong in disease resistance, and is remarkable in production increasing.

The invention discloses a method for breeding a male sterile line by use of the sterile cytoplasm of hibiscus cannabinus KN142, which specifically comprises the following steps: performing saturation backcross by taking a breeding material containing the sterile cytoplasm of hibiscus cannabinus KN142 as a female parent and the cultivated variety of hibiscus cannabinus as a male parent, and selecting a uniform wild abortive type cytoplasmic male sterile line similar to the male parent in character and showing male sterility, wherein the male parent for backcross is a corresponding male sterility maintainer line. The method disclosed by the invention has the beneficial effects that the hybrid matched with the KN142 cytoplasmic male sterile line and the hybrid matched with the UG93 or H040 cytoplasmic male sterile line are popularized and utilized at the same time, and the occurrence risk of certain disease can be greatly reduced.

The present invention relates to plant breeding technology, and is especially anther cultivation technological method of breeding unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. Extracorporeal anther cultivation technology is used in selective breeding of unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. That is, the dual purpose line is obtained through test cross of the fertile plant and sterile plant in contemporary anther cultivation offspring of dual purpose line hybrid, F1 selfing, test cross of similar fertile plant and sterile plant in the offspring lines and continuous two generation selection; and the recovery line is obtained through the test cross of the fertile plant and sterile plant in the contemporary anther cultivation offspring of three line hybrid with available sterile line, selectively breeding the F1 with fertility recovering rate of 100 % and stable fertility to obtain fertile plant as the corresponding recovering plant and final selfing.

The invention discloses a herbicide-resistance-based rape hybrid seed producing method and applications thereof, and relates to the field of plant seed production. The method includes (1) introducing a wide-spectrum type herbicide-resistant gene into a rape male parent line to obtain a herbicide-resistant rape male parent line that is an imidazolidinone-resistant line 12WH381 or a glufosinate-resistant line Z7B10, (2) pollinating a female parent that is sterile after a chemical androcidal action by utilizing pollen of the herbicide-resistant rape male parent line, and harvesting hybrid seeds from male parent plants, and (3) coating the hybrid seeds with a herbicide, or spraying a corresponding herbicide to the hybrid seeds when the hybrid seeds are planted to remove false hybrid seeds at the same time of removing weeds so as to obtain high-purity rape hybrid seeds or group. The applications include (1) hybrid seed production for rape, (2) improvement on rape three-line seed production methods at present, (3) improvement on rape nucleus-sterility two-line seed production methods at present and (4) improvement on rape chemical androcidal seed production systems at present.

The invention belongs to the technical field of vegetable molecular breeding, and particularly relates to an SNP molecular marker related to infertility of capsicum and application thereof. The SNP molecular marker related to the infertility of the capsicum is SNP2, SNP3, SNP4, SNP7 or SNP8, the nucleotide sequence of an SNP site is shown in SEQ ID NO.1-5, and the molecular marker is co-separatedor closely linked to pepper fertility phenotype. The invention also provides a KASP primer for identifying the above SNP molecular marker; the primer can be used for rapidly identifying the pepper fertility phenotype, and can greatly accelerate the transfer process of a novel capsicum male infertility line. The SNP molecular marker related to the infertility of the capsicum developed belongs to acosegregation SNP marker of a nuclear infertility gene, and the plant fertility can be determined in the seedling stage of a pepper, thereby reducing the seed production cost. The marker can also accelerate the transfer process of the novel capsicum male infertility line.

The invention relates to a low-cost matched breeding method for white feather broilers. According to the method, recessive white Plymouth rocks serve as a specialized strain female parent paternal line, high-yield laying hens serve as a specialized strain female parent maternal line, the recessive white Plymouth rocks and the high-yield laying hens are hybridized to generate female parents, and aCornish variety serves as a male parent to be hybridized with the female parents to generate a commercial generation of a specialized strain. Leukemia and pullorum disease full-group general test is preferably carried out on specialized strain progenitor chicken flocks, regular spot test is carried out on the parents, and leukemia individuals and pullorum disease positive individuals are eliminated. Pure line breeding is preferably carried out on the specialized strain paternal line, the female parent paternal line and the female parent maternal line. The breeding cost of the commercial generation is reduced, the growth speed of the commercial generation can be ensured, and the production cost is reduced.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for brassica oleracea, and belongs to the technical field of plant cultivation. Compared with a previous sterile line, a cytoplasmic male sterile line adopted in the breeding and cultivating method has the advantages that the male sterility is thorough, the sterile plant rate and the sterility are respectively 100 percent, the phenomena of low-temperature yellowing and abnormal development of flower organs in a seedling stage are avoided, and the resulting rate is high, and the early-matured and disease-resistant brassica oleracea also has the characteristics of high stress resistance and disease resistance; when a vegetative mass harvested from the brassica oleracea is used for preparing hybrid seeds through the cytoplasmic male sterile line, a recovery line is not required, and only a parent line is required. The method for obtaining the early-matured and disease-resistant brassica oleracea is simple, low in cost and convenient to popularize and has a large market prospect.

The invention relates to a breeding and seed producing method for a recessive gene male sterile line of cauliflower. The invention adopts the technical scheme that a recessive gene male sterile mutant strain of the cauliflower is subjected to sister hybrid and progeny selfing separation to breed a sterile strain with the sterile rate of 100 percent and the sterile degree reaching or approximating to 100 percent; the sterile line used as a female parent is hybridized and combined with an excellent selfing line; and a combined hybrid seed is produced by applying a honeybee pollination method. The invention has the advantages that: firstly, a new sterile source for heterosis advantage utilization of the cauliflower is provided and the defects of cytoplasmic sterility are overcome; secondly, the recessive gene male sterile line is maintained and reproduced by using a tissue culture technology and various works for filed isolation and preproduction of the sterile line and a maintenance line are omitted, so that the influence on preproduction of the sterile line caused by weather, season, land and isolating conditions is avoided; and thirdly, a hybrid strain is produced by using the recessive gene male sterile line and honey can be used for pollination, and thus the seed production cost is reduced and the seed production efficiency is improved.

The invention discloses a tobacco compound pollen medium and a preparation method thereof. The tobacco compound pollen medium is prepared by respectively smashing and evenly mixing the following components in parts by weight: 25-30 parts of glucose, 25-30 parts of cane sugar, 40-49 parts of soluble starch, 0.5 part of sodium borate and 0.5 part of light calcium carbonate. The tobacco compound pollen medium can promote the germination of the pollen and the elongation of pollen tubes, improves the fertilization success rate, improves the tobacco seed yield at per unit, and lowers the seed production cost of the tobacco seeds. The method of the invention has simple operation, low cost, popularization and application values and good economic and social benefits.

The invention relates to the technical field of agriculture and in particular relates to a transgenic element and an application thereof, a method for differentiating a male sterility line and a fertile maintainer line, and an expanding propagation method of the male sterile line of maize. A functional nucleotide sequence controlling the male fertility of the maize and a nucleotide sequence controlling starch synthesis are transferred into the maize together, and then backcross transformation is performed to obtain a hybrid maintainer line. As the nucleotide sequence controlling starch synthesis in the maintainer line inhibits the starch synthesis and increase the sugar content to lead to kernel shrinking, the sterility line and the maintainer line can be doubly differentiated from quality and appearance, and the accuracy is improved. The male sterility line and the fertile maintainer line of the hybrid female parent of the maize can be differentiated accurately and efficiently by use of the method, and then the male sterility line of the hybrid female parent can be applied to expanding propagation and applied to the production of hybrid seeds. Meanwhile, the difference in quality and appearance is more prone to mechanical identification, and therefore, the working efficiency can be greatly improved.

The invention provides a breeding method of entire silo corn hybrids. The breeding method comprises the steps of breeding a parent selfing line, isolating spatially, artificially hybridizing, bagging for isolation and selfing for achieving homozygosity; performing test mating on a selfing line A and a selfing line B to obtain a single cross hybrid F1; and finally, carrying out test mating on the single cross hybrid F1 used as a female parent and a third selfing line C used as a male parent to breed the entire silo corn hybrid (three-way cross hybrid). The breeding method adopts three selfing lines to breed the entire silo corn hybrid and opens up a new method for breeding of the silo corn hybrid; the bred three-way cross hybrid has the characteristics of high quality, high biological yield and high seed production, contains more than 10% of crude protein, lower than 45% of neutral detergent fibers and lower than 25% of acid detergent fibers; two selfing lines are subjected to test mating to obtain the single cross hybrid, then the single cross hybrid used as the female parent and another one selfing line used as the male parent are used for seed production, and therefore, the seed production yield is ensured and the seed production cost can be reduced.

The invention relates to a plant breeding and cultivating method and in particular relates to a stem broccoli breeding and cultivating method, belonging to the technical field of plant cultivation. The method comprises the following steps: performing artificial emasculation to prepare hybrid first generation seeds F1 during flowering phase by utilizing a broccoli breeding material and a cabbage mustard breeding material, and performing selfing on a selected individual plant with excellent comprehensive agronomic traits to obtain F2; screening excellent individual plants, performing microspore culture on flower buds suitable for microspore culture from the late uninucleate stage to early dikaryophase stage, creating lots of normal regeneration plants, screening excellent DH plants for performing selfing, obtaining lots of DH strains with stable botanical traits, high combining ability and large pollen quantity, hybridizing one DH strain serving as a recurrent parent with a cytoplasmic male-sterile source material, breeding to obtain a cytoplasmic male-sterile line CMS091 with excellent comprehensive characters through five-generation backcross transformation, performing trial hybridized combination on the DH strain serving as a male parent and the cytoplasmic male-sterile line CMS091, and obtaining the early ripening disease-resistant stem broccoli.

The invention discloses a seed production method of hybrid rice, which comprises the following steps of: (1) sowing of seeds and culture of seedlings: determining the sowing time according to the sowing periods of a male parent and a female parent to enable the flowering periods of the male parent and the female parent to be synchronized; (2) field management: carrying out water draining and field drying management based on shallow water mainly, covering the water in time after field drying to prevent and eliminate plant diseases and insect pests, and spraying '920' with power-operated spraying and dusting equipment to expel male parent pollen; (3) impurity prevention and preservation of pure state: ensuring that no sticky rice exists in the range of 200 meters beyond a seed production region and only the male parents of the seed production combination can be planted in the range of 20 meters beyond the seed production region, and killing off abnormal plants before flowering of cereal crops and loosing of powder to ensure the seed purity; and (4) harvesting and storing: cutting off the male parents in the seed farm to ensure that no male parent exists in the seed farm, and clearing a harvesting machine in the processes of harvesting and going to the field to ensure that no other seeds and impurities exist in the harvesting machine. The invention has the characteristics of high working efficiency, less labor demands, low cost, high purity, high quality, high yield and the like.

The invention discloses a breeding method for high-resistance and grain-saving high-quality laying hens. A local breed and a dwarf breed of our country are taken as materials, and DW (Dwarf Gene) is introduced into the local breed of our country. A new multiline cross system for high-quality laying hens is established by using the conventional breeding methods combined with molecular assisted marker selection and through strain selection, cross matching and the like, and finally, a new high-resistance and grain-saving high-quality laying hens breed is bred. The technical problem that the cultivation method in the prior art can not realize grain saving and high yield simultaneously is solved.

The invention belongs to the technical field of poultry genetic breeding, and in particular to a method for seed production of automatic sexing high-yield type Luhua feather green shell laying hens. The method for seed production of the automatic sexing high-yield type Luhua feather green shell laying hens adopts non-Luhua feather high-yield laying hens A and non-Luhua feather high-yield laying hens B to form a first paternal line A and a first maternal line B; high-yield, slow feather, non-Luhua feather and non-green shell hens are remainedas a second maternal line A B after the first paternal line A and the first maternal line B are hybridized for pure breeding; fast feather and non-green shell cocks are selected from Luhua chicken varieties and used as male parents, fast feather and green shell hens are selected from non-Luhua chicken varieties and used as female parents, the male parents and the female parents are hybridized for the pure breeding, the fast feather, Luhua feather and green shell gene isozygoty are selected as a final paternal line C; the final paternal line C and the second maternal line A B are hybridized to obtain a commercial generation, wherein the slow feather is a cock and the fast feather is a hen. The automatic sexing high-yield type Luhua feather green shell laying hens obtained from the seed production method has the advantages of high yield, automatic sexing and green eggshells.