Using with cotton sterile male line and recovery line to processing ratoon regenerating hybrid seed production method

A male sterile line and hybrid seed production technology, which is applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve the problem that the yield and quality of hybrid cotton cannot be guaranteed, and it is impossible to sow and harvest seeds multiple times at one time. Survival rate is not equal to the purity of the parents, etc., to achieve the effect of long heterosis maintenance time, easy control of seed production scale, and convenient commercial application

Inactive Publication Date: 2008-02-20
四川省农业科学院经济作物育种栽培研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Production of hybrid cotton seeds by artificial detasseling is labor-intensive and costly
The use of sterile lines to produce hybrid cotton seeds is relatively labor-saving and time-saving, and the cost is reduced, but there are still the following problems: 1. When preparing hybrids every year, it is necessary to sow sterile lines and restorer line parents, and it is not possible to sow multiple harvests at one time. At the same time, it is necessary to remove the hybrid plants of the parents in the field every year, and the seed production is still troublesome. Because the sowing survival rate and the purity of the parents vary from year to year, the scale of seed production is not easy to control.
2. CMS lines and restorer lines also need to be bred every year and provided for hybrid preparation. The annual breeding of the parents themselves is labor-intensive and time-consuming, which further increases the cost and expense of seed production
3. The parents will inevitably produce gene variation and degeneration during their own reproduction process, which will change the special combining ability of the hybrid combination, reduce the heterosis, and the yield and quality of hybrid cotton cannot be guaranteed. Generally, it will be popularized for about ten years. species are severely degraded and must be replaced by new hybrids
The utilization time of heterosis is short, the benefit of hybrid breeding is low, and the cost of hybrid cotton production is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A hybrid seed production method utilizing the regeneration of the perennial root of the complete genital sterile line of cotton. Include the following steps:

[0022] a. In the first year in a seed production area that can survive the winter, that is, where the lowest temperature in winter is ≥10°C, the annual sunshine hours are 1700-2100 hours, the sunshine percentage is 50-60%, and the annual rainfall is 800-1200mm In different areas, the female parent is the cotton genital sterile line, and the male parent is the insect-resistant or non-insect-resistant white cotton line. After flowering, remove the hybrid plants of the parents, and carry out hybridization through artificial assisted pollination or insect pollination, that is, to prepare F1 generation seeds of high-dominant white cotton hybrids for large-area use.

[0023] The seed production fields for preparing hybrids should be separated from the large-scale cotton production fields by more than 100 meters, so as...

Embodiment 2

[0029] A method for producing white cotton hybrids by using two lines of perennial genic male sterile.

[0030] This example is basically the same as Example 1, except that the sterile line used in this example is a nuclear male and female line, and the sterile line plants planted in the first year in step a contain 50% fertile plants , therefore, in step a, also need to increase following operations: from bud stage to present flowering stage, namely after bud stage, before flowering, pull out wherein about 50% of the fertile plants with flower buds, and then use the remaining not The breeding plant is crossed with the male parent through artificial assisted pollination, that is, the F1 generation seed of the highly dominant white cotton hybrid is prepared for large-scale use.

[0031] In the second year and later, there will be no more fertile plants in the sterile line, so there is no need to carry out the above operations.

Embodiment 3

[0033] A method for producing colored cotton hybrids perennially using complete genital sterile lines.

[0034] This example is basically the same as Example 1, except that the restorer line (male parent) is replaced by colored cotton lines such as brown and green. And only repeat steps b and c once, that is, the continuous production of hybrid cotton F1 seeds is a total of 3 years.

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PUM

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Abstract

The invention provides a method of crossbreed seed producing which performs rhizocarpous regeneration by use of the cotton sterile male line and restorer line, and the steps are that: a. first year, in the region where the cotton is able to live through the winter, the cotton male sterile line and the restorer line are respectively planted, the restorer line is used as the male parent to crossbreed with the sterile line to produce the F1 crossbreed cotton seeds so as to being used for the large-area production; b. after gaining the seeds, the sterile line and the restorer line plant live through the winter in natural environment; c. next year, the sterile line and the restorer line plant through the winter are pruned and trained, subsequently, the restorer line is used as the male parent to crossbreed with the sterile line during the flowering period, again the F1 crossbreed cotton seeds are produced so as to being used for the large-area production; d. the two steps of b and c are repeated for 1 to 3 times. The invention promises one year planting and continuously gaining the crossbreed seeds for many years, the invention provides labor and time saving for crossbreed seed production with low cost; moreover, the advantages of the crossbreed can be kept for a long time and the planting output and the quality of the crossbreed cotton can be assured, which is convenient for the commercialized application and has extremely important significance in the popularization of the utilization technology of the cotton crossbreed advantages.

Description

technical field [0001] The invention relates to a hybrid seed production method using cotton male sterility. Background technique [0002] The utilization of heterosis in cotton is one of the effective ways to increase yield, improve quality and increase stress resistance. Research at home and abroad shows that the yield increase of cotton hybrids is generally 5-35%. However, cotton is an allotetraploid often outcrossed crop with a complex genetic background; it also has infinite inflorescences with long flowering periods and scattered flowers; therefore, it is more difficult to study and utilize heterosis than diploid concentrated flowering crops such as rice and corn. Much bigger. Two determinants of heterosis utilization, one is the strength of heterosis, and the other is efficient and simplified hybrid production method. [0003] At present, the production methods of cotton hybrids used in production mainly include: artificial detasseling to produce hybrid cotton seed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 牟方生张小军毛正轩岳福良张超张相琼龚一耘张小红杨泽湖杨益品周洪俊
Owner 四川省农业科学院经济作物育种栽培研究所
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