Using with cotton sterile male line and recovery line to processing ratoon regenerating hybrid seed production method
A male sterile line and hybrid seed production technology, which is applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve the problem that the yield and quality of hybrid cotton cannot be guaranteed, and it is impossible to sow and harvest seeds multiple times at one time. Survival rate is not equal to the purity of the parents, etc., to achieve the effect of long heterosis maintenance time, easy control of seed production scale, and convenient commercial application
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Embodiment 1
[0021] A hybrid seed production method utilizing the regeneration of the perennial root of the complete genital sterile line of cotton. Include the following steps:
[0022] a. In the first year in a seed production area that can survive the winter, that is, where the lowest temperature in winter is ≥10°C, the annual sunshine hours are 1700-2100 hours, the sunshine percentage is 50-60%, and the annual rainfall is 800-1200mm In different areas, the female parent is the cotton genital sterile line, and the male parent is the insect-resistant or non-insect-resistant white cotton line. After flowering, remove the hybrid plants of the parents, and carry out hybridization through artificial assisted pollination or insect pollination, that is, to prepare F1 generation seeds of high-dominant white cotton hybrids for large-area use.
[0023] The seed production fields for preparing hybrids should be separated from the large-scale cotton production fields by more than 100 meters, so as...
Embodiment 2
[0029] A method for producing white cotton hybrids by using two lines of perennial genic male sterile.
[0030] This example is basically the same as Example 1, except that the sterile line used in this example is a nuclear male and female line, and the sterile line plants planted in the first year in step a contain 50% fertile plants , therefore, in step a, also need to increase following operations: from bud stage to present flowering stage, namely after bud stage, before flowering, pull out wherein about 50% of the fertile plants with flower buds, and then use the remaining not The breeding plant is crossed with the male parent through artificial assisted pollination, that is, the F1 generation seed of the highly dominant white cotton hybrid is prepared for large-scale use.
[0031] In the second year and later, there will be no more fertile plants in the sterile line, so there is no need to carry out the above operations.
Embodiment 3
[0033] A method for producing colored cotton hybrids perennially using complete genital sterile lines.
[0034] This example is basically the same as Example 1, except that the restorer line (male parent) is replaced by colored cotton lines such as brown and green. And only repeat steps b and c once, that is, the continuous production of hybrid cotton F1 seeds is a total of 3 years.
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