Propagation method for rice normcore sterile line

Abstract

The invention provides a propagation method for a rice normcore sterile line. The propagation method comprises the following steps of establishing an expression vector containing a color marker gene C, a normcore sterile fertility recovery gene S and a pollen inactivation gene K; converting the expression vector into rice normcore sterile mutant strains to obtain T0 generation transgenic plants; selecting fertile plants, having the three genes of the color marker gene C, the normcore sterile fertility recovery gene S and the pollen inactivation gene K, from the T0 generation transgenic plants; performing self-fertilized propagation on the fertile plants, and separating out engineering maintainer line seeds and normcore sterile line seeds from the seeds obtained from self-fertilized propagation offspring; and taking the normcore sterile line seeds as a female parent while taking the engineering maintainer line seeds as a male parent, performing hybridization on the female parent and the male parent, and obtaining seeds from female parent plants, namely normcore sterile line seeds without transgenic components. The propagation method has the advantages of high occupation proportion of the sterile line seeds, low production cost and the like.

Description

technical field [0001] The invention relates to the technical field of breeding of rice sterile lines, in particular to a breeding method of common nuclear sterile lines of rice. Background technique [0002] Ordinary nuclear sterility is controlled by a pair of recessive nuclear genes, and is generally not affected by external environmental factors. No matter how the environmental conditions change, it always shows infertility. This type of sterility is relatively common in nature. In production and application, common GMS has the following advantages: 1) It is easy to select and breed common GMS lines with 100% sterile rate and sterile plants from common GMS materials; The varieties are all its restorer lines, the restoration spectrum is extremely wide, and the freedom of combination greatly increases the probability of breeding excellent combinations; 3) It can keep up with the pace of conventional rice breeding and open up a new field of heterosis between indica and japo...

AI Technical Summary

Problems solved by technology

[0004] The above two methods can obtain 50% of the sterile line, which breaks through the reproduction problem of common genetic sterile lines and lays the foundation for promoting the large-scale application of common genetic sterile lines. However, the common genetic sterile lines produced by the above methods The breeding line seeds are only half of the current season's production, which increases the production cost of ordinary GMS line seeds, which leads to higher production costs of final hybrid seeds

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