220 results about "Bud growth" patented technology

The invention discloses a double-cropping sophora japonica cultivation and management method. The double-cropping sophora japonica cultivation and management method comprises the steps that after double-cropping sophora japonica seeds are selected and placed into a potassium permanganate solution with the mass concentration of 0.005 percent for soaking treatment, control over bed planting is conducted; then, nursery stock reproduction, garden construction, top grafting for breed changing, fertilizer application, shaping pruning, pest control and sophora japonica flower bud harvesting are managed. According to traditional sophora japonica, the flower bud growth period is long, and the yield is low; by the adoption of the double-cropping sophora japonica cultivation and management method, the flower buds grow in a double-cropping mode and are harvested in the double-cropping mode, so that the yield is increased, and remarkable economic benefit and social benefit are created.

A method for interplanting finger citron along with balsam pear and notoginseng in the North includes such steps as promoting bud growth in the later winter or early spring, breeding, planting in hothouse or hotshed, then cultivating on open field, guiding the vine of balsam pear to the uppon frame, further guiding in due time the vine of finger citron to the lower frame, shading finger citron by balsam pear, spraying water in the morning and at night when the temp. reaches 30-35 deg.C, convering plastic film in the autumn, and planting notoginseng in February of next year.

The invention relates to a tobacco field management method for improving quality of upper tobacco leaves. By the adoption of a cultivation method that the tops of tobacco are sleeved with plastic bags instead of topping, from the first blooming stage to the full blooming stage of the tobacco, all inflorescences together with three to five small tobacco leaves at the topmost portion are sleeved with shading materials instead of traditional manual topping, and therefore flowers and the leaves at the tops of the tobacco gradually die under the high-temperature, high-humidity and dark environment in the bags. Compared with the prior art, the tobacco field management method has the advantages that the apical dominance of the tobacco fades away, nutrients of the tobacco and coordination of chemical components of the tobacco leaves are guaranteed, and the effect of improving the quality of the upper tobacco leaves is obvious; the apical dominance of tobacco plants fades away, growth and development of axillary buds are restrained, the use quantity of suckercides is reduced, and quality safety of the tobacco leaves is improved; if bud picking is carried out manually, the frequency of bud picking is reduced, and labor is relieved; the bags are made of blue or black plastic materials convenient to obtain, cost is low, the bags are cleared away together with tobacco stems later, and no environment pollution is caused.

The invention discloses an induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery, which comprises a bud starting culture medium, a test-tube seedling enrichment medium, a first somatic embryo and / or adventive bud formation culture medium, a second somatic embryo and / or adventive bud formation culture medium, a third somatic embryo and / or adventive bud formation culture medium, a first somatic embryo and / or adventive bud growth and differential medium, a second somatic embryo and / or adventive bud growth and differential medium, and a sound seedling rooting medium. By using the induced rapid propagation culture medium for the somatic embryos of the leaves in vitro of the photinia x frasery, a basal culture medium and content are improved, and the culture condition is adjusted to ensure that the photinia x frasery which is difficult to propagate propagates rapidly through the occurring pathway of the somatic embryos, the plant transplantation survival rate is over 95 percent, a seedling grows healthily, so that the problem of quality reduction of a good seed can be effectively solved, a large amount of high-quality purified and rejuvenated virus-free nursery stocks can be provided for production, and an ideal acceptor system can be provided for genetic transformation or mutation breeding seed selection of the photinia x frasery.

The invention discloses a cherry rootstock tissue culture medium, which is prepared from 2 to 60 ml / L of optimized MS base mother solution, 2545 g / L of sucrose, 6.5 to 7.0 g / L of agar, 0.4 to 0.6 mg / L of 6-Benzylaminopurine, 0.1 to 0.3 mg / L of indolebutyric acid, 0.061 to 0.067 mg / L of VC, 0.05 to1.00 mg / L of indoleacetic acid and 1000 to1200 mg / L of active carbon; the culture medium is specifically applied to the growth and cultivation of the bud of the dwarf cherry rootstock, the cultivation of the strong stock and the cultivation of the induced root; the proliferation and root-growing effect is excellent so that the root-growing rate of the dwarf cherry rootstock can reach 95% to 98%; the root is rough and tidy; the culture bud is healthy; the leaf is green, therefore, the labor and material are effectively saved; the survival rate of the transplant can reach 95%, and the foundation is established for the large-scale production. The tissue culture medium method disclosed by the invention is simple and easy, thereby simplifying the program, improving the efficiency, and reducing the cost. In the invention, the problems of brown stain and vitrification in the culture process of tissue culture medium can be solved; and therefore, the invention can be applied to the fast reproduction of the Gisela sweet dwarf cherry rootstock in the large-scale production.

The invention provides a rapid reproduction method of konjac. In the method, rhizome without flower buds in those years or annual-triennial konjac is induced for flowering by induction of gibberellin (GA3) on the flower buds of the konjac, thus lowering cost of obtaining female parent; and meanwhile the flowering season of male parent is regulated and controlled to meet the flowering season of the induced female parent for creating the chance of pollination by inhibition of low temperature on growth of the flower buds of the konjac. Compared with a natural condition, the method can help rapidly increase the female parent material in quantity with low cost; the male parent can be successively cultivated after low-temperature storage, thus ensuring that the male parent meets the flowering season of the induced female parent; and a great quantity of low-cost konjac seeds can be obtained by hybridization of a small amount of the male parent and a great amount of the induced female parent.

The invention discloses a method for inhibiting tobacco leaf buds, and relates to a method for inhibiting tobacco leaf auxiliary bud growth. The method is as follows: after the tobacco is topped, salt and water are prepared into an aqueous solution according to the mass ratio of 1:8, the aqueous solution is sprayed downwards from the tops of the tobaccos along the tobacco stems, flows to the lower parts of the tobaccos and contacts with auxiliary buds, so that the auxiliary bud young tissues are killed, and the bud inhibiting purpose is realized. According to the method for inhibiting tobacco leaf buds, not only can a bud inhibitor, which is efficient, safe and non-toxic, is not left, is prepared from easily-obtained raw materials and does not pollute, be provided, but also the preparation and use methods are simple and easy to operate, the effect is obvious, the tobaccos are not damaged, and a function of promoting the tobacco leaf production development is obtained. The method is suitable for tobacco leaf cultivation.

The invention belongs to the field of plant biotechnologies and particularly discloses a culture medium and culture method for promoting the growth of regeneration buds of echinacea purpurea. According to the culture medium, DA-6 (diethyl aminoethyl hexanoate) of corresponding concentration is particularly added to an MS formula in accordance with that the regeneration buds of different ploidies have different sensitivities to DA-6, besides adding 15-60g / L of saccharose, 3-9g / L of agar and 0.01-0.2mg / L of NAA (naphthalene acetic acid). The culture medium has the advantage that the height, weight, the weight, diameter and number of roots, the length of primary roots, total root length, root cap ratio and transplanting survival rate of the regeneration buds all can be increased remarkably. By applying the culture medium and the culture method, the development of the scientific research work related to the biotechnological breeding of echinacea purpurea can be promoted.

The invention belongs to a method for genetic transformation and regeneration of a eucalyptus, and particularly relates to the method for genetic transformation and regeneration by taking hypocotyledonary axis of an aseptic seedling of a eucalyptus urophylla as an explant. The hypocotyledonary axis of the seedling of the eucalyptus urophylla is taken as the explant, calluses are pre-cultured and induced, co-culture transformation is performed with agrobacterium tumefactions carrying exogenous genes, kanamycin is used for selection, and adventitious bud induction, bud proliferation, adventitious bud growth, rootage and transplantation are performed for getting a plant to be transformed. PCR (polymerase chain reaction) detection and RT-PCR (reverse transcription-polymerase chain reaction) detection prove that, as for the obtained plant of the eucalyptus urophylla to be transformed, the exogenous genes are introduced into a genome of the eucalyptus urophylla and expressed; the transformed plant having better resistance to phytophthora nicotianae is found in the anti-disease detection of in vitro blades of the eucalyptus urophylla of a reverse radish-anti-fungal protein (RS-AFP2); and the relevant enzyme activity determination result of the resistance shows that, after inoculation of the transformed plant, the activities of L-phenylalanin ammo-nialyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) are enhanced.

The invention belongs to the field of plant biological technologies, and particularly discloses a method for promoting growth of echinacea purpurea regeneration buds. According to the method, according to the biomass of the regeneration buds, the regeneration buds are placed in a growth medium of different DA-6 concentrations to be cultivated, adventitious buds of different biomasses can root well, and when the echinacea purpurea regeneration buds are diploid, the content of DA-6 in the growth medium is 0.08-0.16 mg / L, wherein the adhesion leaf height is 1.5 cm-2.5 cm; when the echinacea purpurea regeneration buds are diploid or triploid, the content of DA-6 in the growth medium is 0.32 mg / L-0.64 mg / L, wherein the adhesion leaf height is 4 cm; when the echinacea purpurea regeneration buds are tetraploid, the content of DA-6 in the growth medium is 0.64 mg / L-1.28 mg / L, wherein the adhesion leaf height of the echinacea purpurea regeneration buds is 4 cm. The height and the root number of the regeneration buds can be increased obviously, and development of related scientific research in echinacea biotechnology seed breeding can be promoted.

The invention discloses an in vitro rapid propagation method for peanuts. On the basis of the prior tissue culture in vitro peanut propagation which comprises the following steps of (1) disinfecting the surface of an explant, (2) inducing adventitious bud formation, (3) elongating and culturing adventitious buds and (4) performing rooting culture, the method optimizes and improves a culture medium for adventitious bud formation and a culture strip for adventitious bud induction in the step (2), as well as a culture medium for bud elongation and culture conditions in the step (3). The method adopts 4-forchlorfenuron as a main component to promote the adventitious bud formation of plant tissue. Compared with the prior matter for promoting the adventitious bud formation, the 4-forchlorfenuron is low in action concentration and remarkable in effects, and explant tissue close to the surface of the culture medium during adventitious bud formation can not appear black, so as to promote bud growth. Compared with the prior method for promoting adventitious bud elongation, the method of the invention has the advantages that the method does not need to increase special equipment, and is low in the using concentration of gibberellin and cytokinin, low in cost, short in needed time for elongation culture and remarkable in effects.

The invention discloses a method applied to tissue culture and rapid propagation of chimonanthus nitens. The method sequentially comprises the following steps: obtaining aseptic seedlings; taking mature chimonanthus nitens fruits and peeling seeds with complete seed coat; inducing callus and inducing adventitious buds; cutting off cotyledon, cutting the cotyledon into small slices with area of 1 square centimeter by a scalpel, inoculating the small slices in a minimal medium; proliferating and seedling the adventitious buds; cutting off the differentiated buds from the callus, and transferring the differentiated buds into a proliferation culture medium to carry out proliferation culture; rooting rootless seedlings; cutting off strong adventitious buds when the adventitious buds grow to be 3-4cm high, and inoculating the strong adventitious buds in a rooting culture medium; hardening and transplanting seedlings of tissue culture regeneration plants; hardening the seedlings when the seedlings of to-be-rooted tissue culture regeneration plants are 5cm high, the root number is greater than 3 and the root length is greater than 5cm, pounding the culture medium and taking out the plants after three days and burying the roots of the regeneration plants in the culture soil. The chimonanthus nitens cultured by the method is capable of quickly rooting and is low in vitrifying and browning rate and high in survival rate.

The invention discloses a method of breeding hills-of-snow Forever Summer quickly by using leaves of a tissue culture seedling. The method comprises the following steps: A) scissoring the third to sixth acrogenous leaves of the sterile tissue culture seedling of Forever Summer and cutting the leaves vertical to the main vein and removing petiole and the tip; B) putting the blades onto a callus induction culture medium for illuminating culture after dark culture; C) after illuminating culture, transferring the blades with callus to a callus proliferation and adventitious bud induction culture medium for subculture to form multiple shoots; D) slitting the multiple shoots, and transferring the multiple shoots to an adventitious bud growth culture medium for elongation growth to form a seedling; and E), cutting the seeding down, and transferring the seedling to a seedling growing and rooting culture medium, wherein the seedling develops an intact regenerated plant with roots, stem and leaves. By adopting the Forever Summer sterile tissue culture seedling leaves to induce callus and differentiating the adventitious bud by the callus, the problems that an explant needs to be sterilized,the pollution is high and the like in the Forever Summer tissue culture process are solved.

The invention relates to the field of plant tissue culture technology, wherein curly pondweed embryo cultivation technique and karren fast breeding method are employed to realize curly pondweed high grade sprout cultivation by means of mass production, so as to recover the fine water ecological condition.

The invention relates to a hyacinthus orientalis L. in-vitro rapid propagation method, and belongs to the field of biotechnology. The hyacinthus orientalis L. in-vitro rapid propagation method comprises the following steps of 1, selecting hyacinthus orientalis L. seed bulbs growing in an open field for 2 to 3 months, carrying out disinfection, and removing outer scales, 2, taking floret petals, flatwise putting the floret petals on a MS solid medium, and carrying out adventitious bud growth induction culture to obtain tissue blocks containing adventitious buds, 3, longitudinally dividing the tissue blocks containing adventitious buds, and carrying out bud elongation culture by a bud elongation medium, and 4, when length values of the adventitious buds obtained by the step 3 are in a range of 2 to 4 centimeters, cutting the adventitious buds, putting the cut adventitious buds into a rooting medium, and carrying out rooting induction culture to obtain hyacinthus orientalis L. seedlings. The young floret petals of hyacinthus orientalis L. are utilized as explants and directly regenerate a mass of adventitious buds and the adventitious buds further develop into regenerated seedlings. Compared with the existing tissue culture method utilizing scales as explants, the hyacinthus orientalis L. in-vitro rapid propagation method has high regeneration efficiency, a high budding rate of about 100% and a large average amount of buds regenerated by induction of each one explant, wherein the large average amount is about 20.

A tissue culture process for mountain tung, essentially comprising the following steps: 1. sterilizing the culture medium under high temperature and pressure, 2. taking the auxiliary bud stem of good seed quality and plant quality as explant, sterilizing with alcohol and corrosive mercury chloride solution, washing with sterilized water, 3. pitching the sterilized explant into culture medium, and putting under artificial daylight lamp for differentiation and bud growth, 4. cutting bud and pitching it into enrichment culture medium, 5. cutting the tube bud and pitching it into sprout-strengthening culture medium, 6. removing the base callus and part of leaves of the tube strengthened sprout, leaving 3-5 leaves and pitching them into root-growing culture medium, 7. taking out the sprout with root for washing, replanting it to culture medium with chaff ash and brown soil, watering thoroughly, shading for 70% in the first 10 days, and then lighting gradually, 20 days later the sprout survivals with root growing out rate being above 85%, and totally lighting 35-45 days later.

The invention relates to a method for producing germinated brown rice by adding a trace amount of nutrient solution. The method is characterized by comprising the following steps: spraying the nutrient solution which contains components such as malic acid, chlorogenic acid and zinc lactate in an atomizing manner until the water content of brown rice is 21-23 percent; then treating the brown rice under a pulse electric field; continuously supplementing the nutrient solution twice, and continuously treating the brown rice under the pulse electric field twice until the water content of rice grains is 13-15 percent to obtain the germinated brown rice with high nutritive value and stable quality. By adding the trace amount of nutrient solution and treating the brown rice under the pulse electric field, the bud growth can be quickly accelerated, and meanwhile, the bacterial growth is suppressed. The method is compact, continuous, and simple and easy to operate.

The invention discloses a method for extracting histocytes from large Michelia alba lateral buds, and relates to the technical field of biological histocyte extraction. The method comprises the following steps: 1, plant culturing; 2, bud cultivation; 3, lateral bud cutting; 4, lateral bud propagation growth; 5, lateral bud growing point embryonic tissue extraction; 6, extraction and purification;and 7, extraction component analysis. Large Michelia alba V3 variety flowered plant segment stalk buds undergo tissue culture, and newborn cells cut from the lateral buds undergo high frequency extraction at 50-60 DEG C in an ultrasonic device with pure water as a solvent.

The invention relate to a method for tissue culture of a pigment marigold in the field of biotechnology, which comprises the following steps: sterilizing marigold leaves and inducing the same, wherein a differential medium used in the induction is specifically MS basic culture medium, an additive comprises 3 mg / L 6-BA, 3 mg / L IAA, 30 g / L sucrose and 6 g / L plant gel, and the culture condition comprises that the temperature is between 23 and 27 DEG C, the illumination time is between 10 and 20 hours per day, the illumination intensity is between 2,000 and 3,000 lux, and the induction time of an adventitious bud is between 9 and 14 days; performing secondary culture on the deferential adventitious bud, wherein the induction bud growth medium is same as the differential medium in the step one, and the culture condition comprises that the temperature is between 23 and 27 DEG C, the illumination time is between 10 and 20 hours per day, the illumination intensity is between 2,000 and 3,000 lux, and the culture time is between 30 and 50 days; and performing rooting culture on the deferential stretched-out bud for 30 days to obtain a marigold regeneration plant. The method has the advantages that materials are convenient to obtain, a large amount of marigold regeneration plants can be induced at a high frequency, the survival rate of the regeneration plants is high, the variation of the regeneration rate is small, and the genetic stability is higher.

The invention relates to a method for increasing the budding and growing consistency of bud grafting seedlings of rubber seeds. The method is characterized in that bud grafting is carried out by taking rubber seedlings which are germinated for1-2 weeks, the top parts of root stocks are cut when second cluster leaves are stable, and water and fertilizer are applied to the root parts of the root stocks before the top parts of the root stocks are cut; after the top parts of the root stocks are cut and before the bud grafting is carried out, culture substrate in a seedling culturing container is maintained to be wet, the water and the fertilizer are applied to root parts of the bud grafting seedlings until the bud grafting seedlings are budded, seedling-strengthening water and fertilizer are applied to the root parts of the bud grafting seedlings after the bud grafting seedlings are budded and leaves are unfolded, and glycine solution is sprayed and applied to the leaf surfaces; after grafted first cluster leaves are stable, and the water and the fertilizer are applied to the root parts of the root stocks until the bud grafting seedlings can be selectively taken out from a nursery after the second cluster leaves are stable; disease and insect damage prevention management is carried out during the period. According to the method for increasing the budding and growing consistency of the bud grafting seedlings of the rubber seeds, disclosed by the invention, the operation is simple and convenient, the efficiency is high, a top advantage removing measure is combined with a tube stroking measure, the problems that the bud grafting seedlings are withered and the budding growth is irregular are effectively solved, the budding and growing consistency of rubber bud grafting seedlings is promoted, convenience is provided for a follow-up growth management, the seedling culturing production cycle is shortened, the seedling culturing production efficiency is increased, and the quality of a nursery stock is increased.

The invention provides a whole-canopy transplantation method of field seedlings of megaphanerophyte. The field seedlings are maintained in situ after primary root breaking is conducted, the broken roots are subjected to treatment of covering of soil balls with sunshade nets, bundling and fixing by means of a rope-form material, sheltering and root protection by means of geotechnical cloth, water absorption and water retention by means of water-absorbing resin, blocking and prevention of excessive evaporation of moisture by means of films, and root promotion and water retention by means of cococoir, interaction is promoted, fast healing of root systems of the seedlings and growth of new buds are promoted, and root system balls with enhanced hardness and compactness and inner-side root systems protected by outer-side root systems are formed on the soil balls of the seedlings, so that sufficient water absorption of young roots is ensured to meet the balance of supply and demand, effectsof reducing the size of the transplantation soil balls, reducing the transportation and maintenance costs and increasing a transplantation survival rate are achieved, and the whole-canopy transplantation method of the field seedlings of the megaphanerophyte is suitable for whole-canopy transplantation of the field seedlings of the megaphanerophyte.

The invention relates to a myriophyllum aquaticum tissue culture and a rapid propagation method thereof. The method comprises the following steps: A) selecting an explant, namely selecting a plant stem with robust growth, bright green color and no plant diseases or insect pests as the explant; B) sterilzing the explant, namely performing treatment on the explant with alcohol and mercury chloride; C) performing bud induction, namely selecting optimal hormone mixture ratio to induce bud growth; D) performing proliferation culture, namely adjusting the hormone mixture ratio and the concentration of a culture medium to perform proliferation culture; E) rooting, namely selecting an appropriate cell auxin to perform rooting induction; and F) hardening and transplanting, namely opening a bottle mouth in a culture box, culturing for two days and then transferring into a natural water body for culture. The technology can not subject to seasonal and environmental restrictions, and a large number of sterile seedlings can be cultured for restoring an aquatic ecosystem.

The invention relates to a regeneration culture method of the whole cotyledonary joint of a hyacinth bean, belonging to the field of modern biotechnology. The method is characterized by comprising the following steps of: (1) sterilizing, soaking and inoculating a hyacinth bean seed to 0-0.5 mg / l of MSB5+6-BA as a germination culture medium to be cultured and germinating an aseptic seedling; (2) removing original leaves, an upper hypocotyl and a radicle from the aseptic seedling, keeping two cotyledons and 3cm of lower hypocotyl, making a micro wound at the joint of the two cotyledons and transferring to 0.6-2.0 mg / l of MSB5+6-BA and 0.2 mg / l of IBA as an adventitious bud inducement culture medium to be induced and germinating; (3) transferring an explant obtained in the step (2) to the adventitious bud inducement culture medium to lengthen; and (4) transferring to 0.1-0.2 mg / l of MSB5+IBA as a rooting culture medium to root and then transplanting to obtain a regeneration plant. The invention has the advantages of large generated adventitious bud quantity, less capping buds, high bud growth speed, short regeneration period, vigorous regeneration plant growth, less tissue culture difference among various seeds and good repetitiveness. The invention is suitable for the tissue culture of various hyacinth bean varieties.

The invention mainly relates to the technical field of planting, and discloses a method for prolonging the Chinese-redbud flowering period. The method includes the steps of topdressing in winter, before-flowering clipping, nutrition spraying and illumination adjusting. The method is simple, operation is convenient, the flowering period of Chinese redbud is kept for 23 days to 25 days, flowers of young and tender branches are flowered by 8 days to 9 days in advance compared with leaves, the flowers extend to the tops of the branches, and are dense and beautiful in color, and the ornamental value of the Chinese redbud is improved; in winter, organic fertilizer is additionally applied to roots of the Chinese redbud, the soil structure is improved, the content of organic matter and the content of oxygen of soil are increased, absorption and storage of nutritional ingredients of the Chinese redbud are promoted, and nutrition preparation is provided for flowering and branches in next spring; in February mid of spring, the Chinese redbud is clipped, old branches are removed, and new branch germination is promoted; meanwhile, nutrients are fully supplied to growth and flowering of the tender branches, the tender branches are girdled, nutrition storage is increased, flower bud growth is promoted, the flower color is bright, the quantity of the Chinese-redbud flowers is increased, and the quality of the Chinese-redbud flowers is improved.

The invention relates to applications of pesticide active ingredient dicamba in inhibiting tobacco axillalry bud growth or in preparing a tobacco bud inhibitor. The invention also discloses a composition comprising another pesticide active component, wherein the pesticide active component is selected from uniconazole, trifluralin, prodiamine, flumetralin, pendimethalin, and butralin; and the weight ratio of dicamba to the pesticide active component ranges from 1:60 to 60:1. The pesticide active component can be made into a soluble liquid preparation, a micron emulsion, a water emulsion preparation, a suspending agent, a water dispersible particle preparation, a microcapsule preparation, a wettable powder, a missible oil, and the like. It is confirmed by experiments that the composition is used for inhibiting bud growth, and bud growth inhibiting effect is excellent, wherein dicamba is taken as the main active ingredient, and the composition is prepared from dicamba, the pesticide active component, and an auxiliary agent; no adverse influence on tobacco leaf chemical composition is caused by application of 40% of dicamba soluble liquid preparation, potassium content is increased, and tobacco leaf quality is improved.

The invention relates to an adjusting and controlling method for concentrated flowering in spring for German chamomile, and the method comprises four processing stages: (1) seed pelleting processing, raising the germinative force, the sprout is neat and consistent; (2) having foliage application in plantlet culture period, applying ammonium nitrogen fertilizer for promoting vegetative growth, applying gibberellins for promoting lateral bud growth; (3) having foliage spray of naphthylacetic acid in big seedling stage for adjusting and controlling the flower bud differentiation; (4) having foliage application of phosphorus potassium fertilizer and ethephon in flowering phase. The ammonium nitrogen fertilizer and the growth regulating agent are applied in the seedling stage for promoting growth, the phosphorus potassium fertilizer and growth regulating agent are applied in flowering phase for promoting the centralized flowering, the flowering phase is 7 to 10 days and the flower yield is 550 kilograms per mu.

The invention provides a plug substrate rainproof strawberry seedling culturing method and a special facility, belonging to the strawberry breeding technology. The method is characterized by comprising the following steps: A. setting up a special shed frame facility, arranging a seedling platform, appressing and covering a permeable nonwoven on substrates in plugs and horizontally arranging a weeping pipe and control valves on the nonwoven at intervals; B. planting parent seedlings and controlling the water content of the substrates at the roots; C. when stem buds on creeping stems of the parent seedlings grow to have two leaves and one core, cutting the permeable nonwoven which is close to the stem bud part and is appressed and covered on the substrates in the plugs, planting the stem buds in each plug by passing through the cut and controlling the water content of the substrates at the roots; and D. when the seedlings continuously grow to the seedling age, namely 30-40 days, controlling the water content of the substrates at the roots to be 10-15% and obtaining annual seedlings when the seedlings grow to have four complete leaves and the external diameters of the dwarf stems are not less than seven millimeters. The method and the special facility have the following beneficial effects that: the annual seedlings are prevented from being infected with diseases and suffering from weeds; water is uniformly supplied and saved; and the water content of the seedlings is effectively controlled and the seedlings are promoted to be ripened more than one month earlier.

The invention relates to an augustine myriophyllum tissue culture and rapid propagation method thereof. The method comprises the following steps: A) selecting an explant, namely selecting a plant stem with robust growth, bright green color and no plant diseases or insect pests as the explant; B) sterilzing the explant, namely performing treatment on the explant with alcohol and mercury chloride; C) performing bud induction, namely selecting optimal hormone mixture ratio to induce bud growth; D) performing proliferation culture, namely adjusting the hormone mixture ratio and the concentration of a culture medium to perform proliferation culture; E) rooting, namely selecting an appropriate cell auxin to perform rooting induction; and F) hardening and transplanting, namely opening a bottle mouth in a culture box, culturing for two days and then transferring into a natural water body for culture. The technology can not subject to seasonal and environmental restrictions, and a large number of sterile seedlings can be cultured for restoring an aquatic ecosystem.

The invention discloses a ceratophyllum demersum L. tissue culture and rapid propagation method. The ceratophyllum demersum L. tissue culture and rapid propagation method includes A, selecting explants, namely, selecting robust clear green plant stem segments without diseases and insect pests as explants; B, sterilizing the explants, namely, treating the explants by alcohol and plant tissue germicide to prepare germfree explants; C, inducing buds, namely, selecting hormone types and hormone concentration combination to induce bud growth; D, performing propagation culture, namely, adjusting hormone combination for propagation culture; E, hardening seedlings and transplanting, namely, opening bottle openings in an incubator, culturing for two days and transferring into natural water for culture. The ceratophyllum demersum L. tissue culture and rapid propagation method has the advantages that the method cannot be limited by seasons and environments, and a great number of aseptic seedlings are cultured for aquatic ecosystem restoration.