Method for promoting growth of echinacea purpurea regeneration buds

A technology of echinacea and regenerated buds, which is applied in the field of plant biology and can solve the problems of uneven growth

Inactive Publication Date: 2015-11-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the problem in the prior art that the regeneration buds of Echinacea purpurea with different biomass grow differently in the medium containing the same concentration of DA-6, the present invention provides a method for promoting the growth of regeneration buds of Echinacea purpurea

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  • Method for promoting growth of echinacea purpurea regeneration buds
  • Method for promoting growth of echinacea purpurea regeneration buds
  • Method for promoting growth of echinacea purpurea regeneration buds

Examples

Experimental program
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Embodiment 1

[0026] This example investigates the effect of different DA-6 concentrations on the growth of diploid regenerated shoots of smaller and medium biomass:

[0027] S1. Preparation of regenerated buds: first obtain the petiole explant of Echinacea purpurea, the petiole explant comes from the diploid aseptic seedling of Echinacea purpurea under tissue culture conditions (the preparation of the explant can refer to the conventional technical method in the art), and then the petiole The explants were inoculated on the regeneration medium (the regeneration medium contained 30g / L sucrose, 0.3mg / LBA, 0.01mg / LNAA, solidified with 4.5g / L agar, in 1.4kgcm -2 Sterilized MS medium for 20 min). When the regenerated bud grows to about 1.5 and 2.5 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated bud.

[0028] S2. Cultivation of regenerated buds: cut out the buds with leaf heights of about 1.5 and 2....

Embodiment 2

[0034] This embodiment has studied the impact of different DA-6 concentrations on the growth of diploid, triploid and tetraploid regenerated buds of larger biomass:

[0035] S1. Preparation of regenerated buds: first obtain the petiole explants of Echinacea purpurea, and the petiole explants are from diploid, triploid and tetraploid aseptic seedlings of Echinacea purpurea under tissue culture conditions (the preparation of explants can refer to this field Conventional technical method), and then inoculate the petiole explants on the regeneration medium (the regeneration medium contains 30g / L sucrose, 0.3mg / LBA, 0.01mg / LNAA, solidified with 4.5g / L agar, in 1.4kgcm -2Sterilized MS medium for 20 min). When the regenerated bud grows to about 4 cm in height with the leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated bud.

[0036] S2. Cultivation of regenerated buds: cut out the buds with a leaf height...

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Abstract

The invention belongs to the field of plant biological technologies, and particularly discloses a method for promoting growth of echinacea purpurea regeneration buds. According to the method, according to the biomass of the regeneration buds, the regeneration buds are placed in a growth medium of different DA-6 concentrations to be cultivated, adventitious buds of different biomasses can root well, and when the echinacea purpurea regeneration buds are diploid, the content of DA-6 in the growth medium is 0.08-0.16 mg/L, wherein the adhesion leaf height is 1.5 cm-2.5 cm; when the echinacea purpurea regeneration buds are diploid or triploid, the content of DA-6 in the growth medium is 0.32 mg/L-0.64 mg/L, wherein the adhesion leaf height is 4 cm; when the echinacea purpurea regeneration buds are tetraploid, the content of DA-6 in the growth medium is 0.64 mg/L-1.28 mg/L, wherein the adhesion leaf height of the echinacea purpurea regeneration buds is 4 cm. The height and the root number of the regeneration buds can be increased obviously, and development of related scientific research in echinacea biotechnology seed breeding can be promoted.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for promoting the growth of regenerated buds of Echinacea purpurea. Background technique [0002] Echinacea ( Echinacea purpurea L.) is a perennial herb of Compositae, which has a good immune regulation effect, and its extract is an immune enhancer and immune regulator that has received widespread attention in the world in recent years. Echinacea has become a world-renowned medicinal plant due to its precise efficacy. In order to obtain multiple varieties of Echinacea purpurea, people usually use tissue culture technology for regeneration or regenerative cloning. Regeneration or regenerative cloning of plants using tissue culture techniques usually involves two steps. The first step is to induce adventitious shoots from explants. The second step is to grow the regenerated shoots into complete plants ready for transplanting. For Echinacea purpurea, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨跃生陈晓鹭李栋梁吴鸿姜伟珍
Owner SOUTH CHINA AGRI UNIV
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