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82results about How to "Solve the survival rate" patented technology

Method for inducing blueberries to root by using liquid culturing media and permeable matrix

The invention discloses a method for inducing blueberries to root by using liquid culture media and permeable matrix, comprising the following steps: (1) preparing rooting culturing media; (2) transferring the healthy and strong blueberry seedlings which are subcultured for 40d and are 3-4 cm high into the rooting culturing media, culturing the blueberry seedlings for 7-10d in the dark and for 12h/d for about 20d under intermitted illumination condition until the blueberry seedlings have 3-7 root systems of 0.5-2 cm long; and (3) transplanting the test-tube blueberry seedlings which are cultured for 30d to root, are 3-6 cm high and have the root length of 0.5-2 cm into the media in which the proportion of turfy soil to moss is 1:1, and managing the blueberry seedlings. The rooting culturing media is prepared according to the following substeps: 1) preparing 0.8 cm*1.2 cm filtering paper balls or defatted cotton balls or 0.2 cm*0.6 cm vermiculites which function as the matrix of the rooting culturing media; 2) preparing rooting culturing solution which has the WPM of 1/2, the IBA of 1.0-5.0 mg.L-1, the edible white sugar of 2 percent and the pH value of 5.0-5.2; and 3) adding 6-10 filtering paper balls or defatted cotton balls or 20-30 vermiculites which are soaked by the rooting culturing solution and adding 3-5 ml rooting culturing solution to each culturing bottle and capping the culturing to sterilize according to the routine method. The invention has short rooting period and low production cost, can ensure that 100 percent of the blueberry seedlings can root and over 95 percent of the transplanted blueberry seedlings can survive and realize the rapid industrialized propagation of the blueberry seedlings on a large scale.
Owner:LUDONG UNIVERSITY

Coilia ectenes step type breeding method

A coilia ectenes step type breeding method comprises the nine steps of reserved parent pool simulated ecological cultivation, parent pool intensive culture, parent selection for induced spawning, parent induced spawning, natural insemination, germ cell collection, germ cell incubation, indoor intensive fry rearing in a cement pool and large-scale fry culture in an outdoor pool. The method is characterized in that filial-generation coilia ectenes adult fishes which are more than 2 years old and cultured by manpower are used as reserved parents for artificial propagation, and a natural ecology cultivation mode is simulated; the selected parents are transported and placed in an induced spawning pool, after parent induced spawning is conducted, the germ cells are collected, counted and transferred to an incubation pool, after the germ cells are collected, the germ cells are placed in the incubation pool with water, fries open the mouths 5-6 days after membrane rupture is conducted, yolks and freshwater rotifers which are filtered through a 80-mesh silk net are fed to the fries, after the fries are cultivated for 15 days, the fries are divided to different pools, and when the fries are as long as 20-30 mm, the fries are placed to outdoor pools to be cultivated.
Owner:上海市水产研究所(上海市水产技术推广站)

Wild jujube stock immature stem grafting seedling culturing method for jujube tree in solar greenhouse

The invention provides a wild jujube stock immature stem grafting seedling culturing method for a jujube tree in a solar greenhouse. The method includes the following steps of A, scion preparation; B, seedling culturing, wherein wild jujube seeds are sown in the first ten-day period of January for seedling culturing, a wild jujube stock is stumped in early of middle of May, seedlings are grafted at the rootstock position of the wild jujube stock, the seedlings are cultured in mid-to-late October, and solar greenhouse nutrition bags are adopted for seedling culturing; C, manufacturing of the nutrition bags; D, manufacturing of a seedbed; E, seed treatment; F, sowing, wherein when white parts of 30% of the wild jujube seeds in the step E are exposed, sowing is conducted, sowing is selected to be conducted on sunny days, bottom water is sufficiently irrigated to the seedbed before sowing, the bed surface is slicked before the water completely seeps, three or four seeds are dibbled in each nutrition bag after the water completely seeps, the nutrition bags are covered with nutrient soil of 2 cm to 3 cm, and heat and moisture preservation is conducted; G, seedling management; H, grafting, wherein jujube tree scions are grafted on the wild jujube stock immature stem through a split grafting method; I, grafted seedling management; J, disease and pest prevention and control; K, nursery stock outplanting. The jujube tree adult seedling outplanting time is shortened, and the survival rate is high.
Owner:郝哲

Biological compound soil conditioner and preparation method thereof

The invention discloses a biological compound soil conditioner and a preparation method thereof. The soil conditioner is prepared from the following raw materials: 5-20% of urea, 5-20% of ammonium dihydrogen phosphate, 1-4% of mycorrhizal fungi and the balance of active lignite. The preparation method comprises the steps of (1) crushing the active lignite into solids with wide range particles; (2) preparing a urea saturation solution; (3) preparing an ammonium dihydrogen phosphate saturation solution; (4) mixing and stirring active lignite particles with the urea saturation solution and the ammonium dihydrogen phosphate saturation solution; (5) ventilating and drying the evenly stirred mixture at a room temperature; (6) stirring and mixing the dried mixture and the mycorrhizal fungi evenly to obtain the biological compound soil conditioner; and (7) hermetically packaging the biological compound soil conditioner. The preparation method is simple; the produced biological compound soil conditioner has the characteristics of high biological activity, excellent adsorptive property and low cost, multiple nutrients for plant growth can be provided and the technical problems of serious water and fertilizer losses and a low plant survival rate in the soil conditioning process are solved.
Owner:HUANENG CLEAN ENERGY RES INST +1

Feed additive capable of improving survival rate of hybrid snakehead fingerlings as well as feed and preparation method of feed additive

The invention discloses a feed additive capable of improving survival rate of hybrid snakehead fingerlings as well as a feed and a preparation method of the feed additive. The additive is prepared from a functional additive A and a functional additive B, wherein the functional additive A is mainly prepared from the following raw materials in parts by weight: 20 to 40 parts of compound Chinese medicinal herb extract, 15 to 30 parts of compound amino acid, 8 to 16 parts of taurine, 10 to 20 parts of beer yeast extract, 5 to 15 parts of betaine and 5 to 10 parts of mannan oligosaccharides; the functional additive B is a micro-ecological preparation and accounts for 10 percent to 20 percent of the total weight of the additive. The additive disclosed by the invention has a feeding attraction effect and is used for accelerating conversion of feeding habits of the hybrid snakehead fingerlings, promoting digestive tract development of the fingerlings and improving the digestion and absorption rate of nutrient substances of the feed so as to promote the growth of the fingerlings; furthermore, the additive can also be used for improving the organism immunity of the fingerlings and reducing the bursting of diseases. When the additive is added into the feed, the survival rate and the weight gain rate of the fingerlings at a feeding habit conversion phase can be improved; a preparation process is simple and the cost is low.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY

Method for planting forage grass on sunward side of hillside land with slope greater than 15 degrees in alpine mountainous area

The invention relates to a method for planting forage grass on a sunward side of hillside land with the slope greater than 15 degrees in an alpine mountainous area. The method comprises the steps thatthe forage grass is planted in a rotation place in the alpine mountainous area with the altitude of 2,000-2,500 m in the southwestern Yunnan province of China, the sunward-side hillside land with theslope greater than 15 degrees is selected as the rotation place, and after former crops are harvested, the forage grass is planted; forage grass planting is conducted by adopting the mode that 9-11%of annual ryegrass seeds, 18-22% of perennial ryegrass seeds, 9-11% of hybrid ryegrass seeds, 28-32% of dactylis glomerata seeds, 28-32% of trifolium repens seeds and base fertilizer are mixed and sowed, and grazing can be conducted when the forage grass grows till the height of a grass layer exceeds 5 cm; when the forage grass is surplus, the harvested forage grass is dried and prepared into green hay or silage, and the stubble height during grass harvesting is 5 cm. The method for planting the forage grass in the alpine mountainous area has the advantages that the method is suitable for being implemented in the southwestern Yunnan province of China, and the forage grass is quick in propagation, good in growth and significant in ecological restoration effect.
Owner:开远市畜牧技术推广站

Seed introduction method of submerged plant vallisneria

The invention discloses a seed introduction method of submerged plant vallisneria, which comprises the steps of collecting ripening fruits of the vallisneria, removing epicarp, obtaining seeds, washing the seeds with clean water within 0-24h to keep the seeds moist, transporting the collected seeds to a destination by a water-culture method, mixing the seeds transported to the destination with moist river sand, refrigerating the seeds at 0-10 DEG C, preserving the seeds by shading above 95% till complete darkness, ventilating and replenishing water frequently, maintaining a moisture content of each seed to be 35-50%, sowing the preserved seeds in a mixed matrix, a soil matrix or a yellow clay matrix, controlling a temperature to be 10-30 DEG C, the illumination intensity to be 800-3500LUX, and the everyday illumination time to be 10-12h, allowing the seeds to germinate and grow, obtaining germinated seedlings, planting the germinated seedlings in the mixed matrix with a 10-15cm water level for culturing, managing other conditions according to a convention, planting the vallisneria, and allowing the vallisneria to grow. The mixed matrix comprises the following components by total mass fraction: 20-40% of sand and 60-80% of soil. The seed introduction method is long in seed preservation time, high in germination rate, and high in emergence rate and planting survival rate.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

Water and fertilizer management method for rubber tree container seedling

InactiveCN104351002ASolve the problem of labor-intensive seedling cultivationSolve the waste of water and fertilizerClimate change adaptationWatering devicesDrip irrigationSlurry
The invention relates to the technical field of cultivation of forest tree seedlings, in particular to a water and fertilizer management method for a rubber tree container seedling. The method comprises the following steps: uniformly mixing coco coir, farmland topsoil and farmyard manure as a cultivation substrate to be put into a seedling raising container; vertically placing the seedling raising container on a bracket, getting the bottom of the seedling raising container away from the ground, and transplanting the rubber tree seedling; irrigating the rubber tree seedling with clear water once through drip irrigation to keep the water mass fraction of the cultivation substrate above 80 percent; irrigating water and a fertilizer once every 2-15 days through drip irrigation, wherein the amount of the fertilizer applied for each plant is 30-60 ml of farmyard biogas slurry manure or 0.5-1 g of a compound fertilizer, and the nutrition supply of the plant is guaranteed until to out-planting of the rubber tree seedling. The method is suitable for cultivating full stands with complete developed root systems and longer main roots; the problems of more labor for seedling raising of the container seedling, waste of water and fertilizer, low planting survival rate and the like for a long time are solved.
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI

Staphylea bumalda tender branch cutting seedling cultivation method

The invention discloses a Staphylea bumalda tender branch cutting seedling cultivation method. The method includes the first step of cutting seedling base facilities, the second step of a cutting matrix, the third step of cutting selection and cutting shoot preparation, the fourth step of cutting, the fifth step of post-cutting management, the sixth step of disease and pest prevention, and the seventh step of seedling selling. By means of seedlings cultivated by conducting root promotion on cutting shoots and processing ends of cutting shoots, the quality and resistance of cutting seedlings are improved, and the plantation period of Staphylea bumalda can be greatly shortened by planting young seedlings of cutting seedling cultivation. Meanwhile, by means of the tender branch cutting seedling cultivation technology, excellent characteristics of mothers are easily ensured, local material utilization, local seedling cultivation and local plantation can be easily conducted, and the advantages of being rapid in growth, high in yield, high in adaptability and the like of seedlings are fully shown after plantation. In addition, high-quality strong seedlings are cultivated through scientific management of cutting seedling cultivation, young seedlings subjected to cutting grow neatly, the seedling disease rate is decreased, the economic value of seedlings and the survival rate of seedling culture can be increased, and social and economic benefits are improved.
Owner:徐华龙

Schizothorax waltoni anesthesia method

InactiveCN108324708AFix the injurySolve the technical problems of sickness and even deathOrganic active ingredientsClimate change adaptationDiseaseWater source
The invention discloses a schizothorax waltoni anesthesia method and relates to application of the method in schizothorax waltoni long-distance transport and short-time water-leaving practice of schizothorax waltoni. The method includes steps: 1) anesthesia water source preparation A, to be more specific, adding a water source at the same temperature with water in a schizothorax waltoni aquaculture pond into an anesthesia container, wherein the temperature is 15-20 DEG C, and dissolved oxygen content is larger than or equal to 5mg/L; 2) MS-222 anesthesia liquid preparation B, to be more specific, adding MS-222 powder into the water source obtained in the step 1) to prepare anesthesia liquid; 3) schizothorax waltoni anesthesia C, to be more specific, subjecting schizothorax waltoni to anesthesia in the anesthesia liquid; 4) after-anesthesia recovery, to be more specific, enabling schizothorax waltoni to recover to normally swim for 3-6min in clear water after anesthesia. The method is feasible, simple and convenient to operate and effective in anesthesia effect, and problems of disease resistance decline and low survival rate due to stress reaction of schizothorax waltoni in transport and water-leaving process are solved.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Method for repairing contaminated soil by using natural pore soil-conserving and planting device

The invention discloses a method for repairing contaminated soil by using a natural pore soil-conserving and planting device. The method comprises the following steps: (1) selecting natural porous medium after airing, including sundried loofah sponge; (2) taking a section of sundried loofah sponge, filling by nutrient soil after taking a cavity, and planting the plant or seeds for repairing petroleum- or heavy metal-polluted soil into the natural pore soil-conserving and planting device formed by the loofah sponge, so as to ensure that the fibrous root can pass through the outside of the planting device and freely grow towards the periphery, and the length of the section of loofah sponge is 6-15cm. The plant root can absorb harmful substances such as petroleum hydrocarbon in soil, meanwhile, microorganisms on the fibrous root can degrade petroleum hydrocarbon and the like, and the planting device is planted in the repair region and prompted to grow, so that the petroleum-contaminated soil is quickly repaired. By adopting the method, the problems of low efficiency and low survival rate in the traditional repair of the petroleum-contaminated soil by the organisms are solved, the used material is a natural plant material, and secondary pollution to the repaired environment is avoided.
Owner:湖南晨露环保科技发展有限公司

Method for transplanting and hardening tissue culture seedlings of rabdosia nervosa

The invention belongs to the technical field of plant tissue culture, and particularly discloses a method for transplanting and hardening tissue culture seedlings of rabdosia nervosa. The method for transplanting and hardening the tissue culture seedlings of the rabdosia nervosa comprises the following steps of (1) selecting seedlings with roots; (2) performing pretreatment before transplanting; (3) proportioning transplanting mediums; and (4) controlling a seedling-hardening environment: putting the transplanted rabdosia nervosa into a greenhouse for seedling hardening, and adjusting and controlling light, temperature and humidity. The method can effectively solves the phenomena that water losses are severe, the survival rate is low and the commodity rate is low during transplanting and seedling hardening of the rabdosia nervosa; by proportioning the different mediums, cost can be reduced, the seedling transplanting flow is simplified, and the survival rate of seedling transplanting reaches up to 95%; by controlling external conditions of seedling hardening after transplanting, the problem that the water losses of the tissue culture seedlings of the rabdosia nervosa are severe canbe effectively solved, and thereby, commodity rate of the seedlings is increased; the market requirements can be effectively met; and a plenty of high-quality rabdosia nervosa seedlings are rapidly supplied for markets.
Owner:WUXI MISHO ECOLOGY LANDSCAPE
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