Method for inducing blueberries to root by using liquid culturing media and permeable matrix

Abstract

The invention discloses a method for inducing blueberries to root by using liquid culture media and permeable matrix, comprising the following steps: (1) preparing rooting culturing media; (2) transferring the healthy and strong blueberry seedlings which are subcultured for 40d and are 3-4 cm high into the rooting culturing media, culturing the blueberry seedlings for 7-10d in the dark and for 12h/d for about 20d under intermitted illumination condition until the blueberry seedlings have 3-7 root systems of 0.5-2 cm long; and (3) transplanting the test-tube blueberry seedlings which are cultured for 30d to root, are 3-6 cm high and have the root length of 0.5-2 cm into the media in which the proportion of turfy soil to moss is 1:1, and managing the blueberry seedlings. The rooting culturing media is prepared according to the following substeps: 1) preparing 0.8 cm*1.2 cm filtering paper balls or defatted cotton balls or 0.2 cm*0.6 cm vermiculites which function as the matrix of the rooting culturing media; 2) preparing rooting culturing solution which has the WPM of 1/2, the IBA of 1.0-5.0 mg.L-1, the edible white sugar of 2 percent and the pH value of 5.0-5.2; and 3) adding 6-10 filtering paper balls or defatted cotton balls or 20-30 vermiculites which are soaked by the rooting culturing solution and adding 3-5 ml rooting culturing solution to each culturing bottle and capping the culturing to sterilize according to the routine method. The invention has short rooting period and low production cost, can ensure that 100 percent of the blueberry seedlings can root and over 95 percent of the transplanted blueberry seedlings can survive and realize the rapid industrialized propagation of the blueberry seedlings on a large scale.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture rapid propagation, in particular to a method for inducing rooting of bilberry test tubes by means of a liquid medium attached with a permeable matrix. Background technique [0002] Bilberry has another name called blueberry and blue berry, and it is a small berry fruit tree of the genus Vaccinium in the Rhododendronaceae (Ericaceae). Bilberry fruit has delicate pulp, very small seeds, sweet and sour taste, and refreshing and pleasant aroma. The unique bilberry anthocyanins, arbutin, SOD and other substances in the fruit have the effects of improving eyesight, anti-aging, eliminating fatigue and preventing cancer. Both Japan and the United States list bilberry in the forefront of anti-cancer foods, known as "21st Century Functional Health Berries" and "Queen of Fruits". Bilberry is not only a good product for fresh food, but also can be further processed into high value-added product...

AI Technical Summary

Problems solved by technology

[0003] Bilberry has been improved, cultivated and planted in the United States and Canada for nearly a hundred years. my country began to introduce excellent varieties of bilberry from the United States and Canada in the 1990s, and carried out research on rapid propagation of seedlings, but the expansion and promotion of conventional breeding methods is slow. , the research on rapid propagation of tissue culture in vitro, most authors report: Vaccinium test tube rooting difficulties, low rooting rate, long rooting cycle, low transplanting survival rate (Rowland et al., 1992; Dweikate et al., 1998; Wang Xiali, etc. , 2003; Liu Shuying et al., 2004; Tao Jianmin et al., 2006), restricting the industrialized seedling raising process of good varieties, therefore, it is very important to select a kind of efficient and fast induced rooting method for the rapid propagation and popularization of bilberry