Schizothorax waltoni anesthesia method

A technology of schizothorax and anesthesia solution, which is applied in the directions of anesthetics, fish farming, pharmaceutical formulations, etc., can solve the problems of low survival rate, decreased disease resistance, etc., and achieves the effects of easy operation, simple operation and stress relief.

Inactive Publication Date: 2018-07-27
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of Lhasa Schizothorax anesthesia method, the method is easy to implement, simple and convenient to operate, the anesthesia effect is good, recovers quickly after anesthesia, solves the disease resistance of Lhasa Schizothorax due to stress response in the artificial manipulation process The problem of decreased strength and low survival rate

Method used

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  • Schizothorax waltoni anesthesia method
  • Schizothorax waltoni anesthesia method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] according to figure 1 As can be seen, a kind of Lhasa Schizothorax anesthesia method, its step is:

[0039] 1) Preparation of anesthesia water source A: Add water source at the same temperature (15-20°C) as the Lhasa Schizothorax breeding pond in the anesthesia container, dissolved oxygen 5 or 6 or 7 or 8 or 10 mg / L; the anesthesia container is smooth on the inner wall The rectangular plastic box has a length of 60 or 70 or 80 cm, a height of 40 or 45 or 50 cm, and an anesthesia liquid water depth of 25 or 28 or 30 or 33 or 35 cm.

[0040] 2) Preparation B of MS-222 anesthesia solution: add MS-222 powder to the water source obtained in step 1) to prepare an anesthesia solution; the anesthetic is MS-222 (powdered fish diazepam) with a mass percentage of 99.0%; the The prepared concentration of the anesthesia solution is 30 or 34 or 38 or 40 mg MS-222 per liter of water, that is, the concentration of the anesthesia solution is 30 or 34 or 38 or 40 mg / L.

[0041] 3) Anes...

Embodiment 2

[0045] The application of a Lhasa Schizothorax anesthesia method in Lhasa Schizothorax simulated transportation, the steps are as follows:

[0046] 1) Preparation of anesthesia water source A: After cleaning and disinfecting the anesthesia water tank, add water at the same temperature (16-17°C) as the Schizothorax culture pond in Lhasa. The dissolved oxygen in the water source is 5.3 mg / L, and the pH is controlled at 7.68. The anesthesia water tank is a plastic box with a smooth inner wall, 70cm long, 45cm wide, and 50cm high;

[0047] 2) Add anesthetic agent to the water source obtained in step 1), and obtain anesthetic solution evenly, wherein, the anesthetic agent is MS-222 (powdered fish diazepam) with a mass percentage of 99.0%, add 40mg MS-222 in every liter of water, mix Homogenize the anesthetic solution with a concentration of 40mg / L;

[0048] 3) In order to simulate the transportation conditions, first put the anesthesia solution into double-layer nylon bags, and fi...

Embodiment 3

[0052] The application of a Lhasa Schizothorax anesthesia method in the practice of Lhasa Schizothorax short-term out-of-water manipulation, the steps are as follows:

[0053] 1) After cleaning and disinfecting the anesthesia water tank, add water at the same temperature (16-17°C) as the Schizothorax culture pond in Lhasa. The dissolved oxygen in the water source is 5-6 mg / L, and the pH is controlled at 7.7 or 7.9 or 8.0. The water tank is a smooth plastic box with a length of 70cm, a width of 45cm and a height of 50cm;

[0054] 2) Add anesthetic agent to the water source obtained in step 1), stir well to obtain anesthetic solution, wherein the anesthetic agent is MS-222 (powdered fish diazepam) with a mass percentage of 99.0%, add 40mg MS-222 in every liter of water, Mixed into an anesthetic solution with a concentration of 40mg / L;

[0055] 3) Put the Lhasa Schizothorax with an average weight of 100g into the anesthesia box for anesthesia. After 2-4 minutes, the Lhasa Schizo...

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Abstract

The invention discloses a schizothorax waltoni anesthesia method and relates to application of the method in schizothorax waltoni long-distance transport and short-time water-leaving practice of schizothorax waltoni. The method includes steps: 1) anesthesia water source preparation A, to be more specific, adding a water source at the same temperature with water in a schizothorax waltoni aquaculture pond into an anesthesia container, wherein the temperature is 15-20 DEG C, and dissolved oxygen content is larger than or equal to 5mg/L; 2) MS-222 anesthesia liquid preparation B, to be more specific, adding MS-222 powder into the water source obtained in the step 1) to prepare anesthesia liquid; 3) schizothorax waltoni anesthesia C, to be more specific, subjecting schizothorax waltoni to anesthesia in the anesthesia liquid; 4) after-anesthesia recovery, to be more specific, enabling schizothorax waltoni to recover to normally swim for 3-6min in clear water after anesthesia. The method is feasible, simple and convenient to operate and effective in anesthesia effect, and problems of disease resistance decline and low survival rate due to stress reaction of schizothorax waltoni in transport and water-leaving process are solved.

Description

technical field [0001] The invention belongs to the technical field of fish anesthesia, in particular to an anesthesia method for Schizothorax Lhasa, which is mainly suitable for short-term out-of-water manipulation and medium-to-long-distance transportation of Schizothorax Lhasa. Background technique [0002] Lhasa Schizothorax (Schizothorax waltoni Regan, 1905) belongs to Cyprinidae Schizothorax, and its local name is "Jianzui". Historically, Lhasa Schizothorax has always been the dominant fish and economic fish in the producing area. However, in recent years, with the intensification of human activities such as tourism and fishing, the resources of Lhasa Schizothorax have declined significantly. For this reason, many domestic units have carried out research on protection technologies such as artificial domestication and breeding, multiplication and release of Lhasa Schizothorax. In the process of carrying out various research practices, it is inevitable to carry out ope...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/245A61P23/00A01K61/10
CPCA61K31/245A01K61/10Y02A40/81
Inventor 朱挺兵龚进玲吴兴兵杨德国
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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