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Tissue culture rapid propagation system of European hornbeam

A technique for tissue culture fast propagation and hornbeam, which is applied in the field of plant tissue culture, can solve the problems of low rooting rate, difficult rooting, gaps and the like, and achieves the effect of high rooting rate

Inactive Publication Date: 2013-07-03
JIANGSU ZITENG HORTICULTURAL GREENING ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

European hornbeam cuttings are difficult to root, yellowing, blanching, and binding treatments can improve the rooting rate of the cuttings, but the rooting rate is still low
There were few reports on the tissue culture and rapid propagation of European hornbeam in the past. Only a few scholars used stem segments and terminal buds as explants to obtain complete plants in vitro and rapidly propagated European hornbeam. The industrialized seedling cultivation has laid a certain theoretical foundation, but there is still a certain gap from the industrialized seedling cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Select well-grown shoot tips and inoculate them on the induction medium supplemented with 0.5mg / L BA after surface disinfection; the basic medium is WPM, and the basic medium also contains 6.5g / L agar powder and 30.0g / L sucrose. After adventitious buds grow out, cut 1.5-2.0cm stem segments for proliferation, the basic medium is MS; the additional plant hormones are 0.1mg / L of BA, 0.5mg / L of KT and 0.01mg / L of IBA; Contains 6.0g / L of agar powder and 30.0g / L of sucrose. 60 days to count the multiplication factor. Cut out healthy single seedlings with a length of 3~4cm for rooting culture, the basic medium is 1 / 2WPM; the additional plant hormones are 0.01mg / L NAA, 0.5mg / L IBA; also contains 6.5g / L Agar powder and 15.0g / L sucrose, pH 5.8~6.0, light 12h / d, light intensity 2000 lx. After 60 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that each explant had an average of 2.8...

Embodiment 2

[0019] Select well-grown shoot tips and inoculate them on the induction medium supplemented with 2.0mg / L BA after surface disinfection; the basic medium is WPM, and the basic medium also contains 6.5g / L agar powder and 30.0g / L sucrose. After adventitious buds grow out, cut 1.5-2.0cm stem segments for proliferation, the basic medium is MS; the additional plant hormones are 1.0mg / L of BA, 2.0mg / L of KT and 0.01mg / L of IBA; Contains 6.0g / L of agar powder and 30.0g / L of sucrose. 60 days to count the multiplication factor. Cut out healthy single seedlings with a length of 3~4cm for rooting culture, the basic medium is 1 / 2WPM; the additional plant hormones are 0.5mg / L NAA and 0.5mg / L IBA; it also contains 6.5g / L Agar powder and 15.0g / L sucrose, pH 5.8~6.0, light 12h / d, light intensity 2000 lx. After 60 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that each explant had an average o...

Embodiment 3

[0021] Select well-grown shoot tips and inoculate them on the induction medium supplemented with 1.0mg / L BA after surface disinfection; the basic medium is WPM, and the basic medium also contains 6.5g / L agar powder and 30.0g / L sucrose. After adventitious buds grow out, cut 1.5~2.0cm stem segments for proliferation, the basic medium is MS; the additional plant hormones are 1.0 mg / L of BA, 0.5 mg / L of KT and 0.5 mg / L of IBA; Contains 6.0g / L of agar powder and 30.0g / L of sucrose. 60 days to count the multiplication factor. Cut out healthy single seedlings with a length of 3~4cm for rooting culture, the basic medium is 1 / 2WPM; the additional plant hormone is 0.01mg / L NAA; it also contains 6.5g / L agar powder and 15.0g / L sucrose, pH 5.8~6.0, light 12h / d, light intensity 2000 lx. After 60 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that each explant had an average of 4.06 adventit...

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Abstract

The invention relates to a tissue culture rapid propagation system of European hornbeam, which is characterized by comprising the following process steps of: selecting a stem tip which fully grows and inoculating onto an induction medium after surface sterilization; culturing for 4 weeks and systemically calculating the induction rate; shearing a 1.5-2.0-cm stem segment, inoculating onto a proliferation medium and systemically calculating the proliferation times after 60 days; shearing a 3-4-cm long single seedling which grows strongly, inoculating onto a rooting medium and inducing rooting; culturing for 50 days and systemically calculating the rooting rate, the average root number and the average root length; and when the root length is 3-5 cm, hardening a seedling and transplanting, and investigating the transplanting survival rate of the seedling after 30 days. The tissue culture rapid propagation system has the advantages that a European hornbeam test tube seedling can be induced to quickly proliferate and generate a large amount of adventitious buds, the survival rate is up to 85 percent, and an effective way is provided for industrial seedling culture of the European hornbeam.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of European hornbeam, belonging to the field of plant tissue culture. Background technique [0002] European hornbeam ( Carpinus betulus ) Also known as Western Elm, a deciduous tree belonging to the genus Betulaceae (Betulaceae) ( Carpinus L.), mainly distributed in Europe, Iran, Turkey. It has dense branches and leaves, compact plant type, beautiful leaf shape, golden leaves in autumn, and strange fruit ears, which are quite beautiful. It is a good tree species for roads, urban green spaces and greening around. In addition, it has many branches and is extremely resistant to pruning. It can be artificially shaped to form various shapes to enrich the plant landscape. For example, it was often pruned into hedges in early British and American gardens. And because of its strong cold resistance, strong wind resistance, wide adaptability, and less pests and diseases, it is an ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 祝遵崚金建邦徐惠群仲秀林
Owner JIANGSU ZITENG HORTICULTURAL GREENING ENG CO LTD
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