Tissue culture rapid propagation system of European hornbeam
A technique for tissue culture fast propagation and hornbeam, which is applied in the field of plant tissue culture, can solve the problems of low rooting rate, difficult rooting, gaps and the like, and achieves the effect of high rooting rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0017] Select well-grown shoot tips and inoculate them on the induction medium supplemented with 0.5mg / L BA after surface disinfection; the basic medium is WPM, and the basic medium also contains 6.5g / L agar powder and 30.0g / L sucrose. After adventitious buds grow out, cut 1.5-2.0cm stem segments for proliferation, the basic medium is MS; the additional plant hormones are 0.1mg / L of BA, 0.5mg / L of KT and 0.01mg / L of IBA; Contains 6.0g / L of agar powder and 30.0g / L of sucrose. 60 days to count the multiplication factor. Cut out healthy single seedlings with a length of 3~4cm for rooting culture, the basic medium is 1 / 2WPM; the additional plant hormones are 0.01mg / L NAA, 0.5mg / L IBA; also contains 6.5g / L Agar powder and 15.0g / L sucrose, pH 5.8~6.0, light 12h / d, light intensity 2000 lx. After 60 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that each explant had an average of 2.8...
Embodiment 2
[0019] Select well-grown shoot tips and inoculate them on the induction medium supplemented with 2.0mg / L BA after surface disinfection; the basic medium is WPM, and the basic medium also contains 6.5g / L agar powder and 30.0g / L sucrose. After adventitious buds grow out, cut 1.5-2.0cm stem segments for proliferation, the basic medium is MS; the additional plant hormones are 1.0mg / L of BA, 2.0mg / L of KT and 0.01mg / L of IBA; Contains 6.0g / L of agar powder and 30.0g / L of sucrose. 60 days to count the multiplication factor. Cut out healthy single seedlings with a length of 3~4cm for rooting culture, the basic medium is 1 / 2WPM; the additional plant hormones are 0.5mg / L NAA and 0.5mg / L IBA; it also contains 6.5g / L Agar powder and 15.0g / L sucrose, pH 5.8~6.0, light 12h / d, light intensity 2000 lx. After 60 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that each explant had an average o...
Embodiment 3
[0021] Select well-grown shoot tips and inoculate them on the induction medium supplemented with 1.0mg / L BA after surface disinfection; the basic medium is WPM, and the basic medium also contains 6.5g / L agar powder and 30.0g / L sucrose. After adventitious buds grow out, cut 1.5~2.0cm stem segments for proliferation, the basic medium is MS; the additional plant hormones are 1.0 mg / L of BA, 0.5 mg / L of KT and 0.5 mg / L of IBA; Contains 6.0g / L of agar powder and 30.0g / L of sucrose. 60 days to count the multiplication factor. Cut out healthy single seedlings with a length of 3~4cm for rooting culture, the basic medium is 1 / 2WPM; the additional plant hormone is 0.01mg / L NAA; it also contains 6.5g / L agar powder and 15.0g / L sucrose, pH 5.8~6.0, light 12h / d, light intensity 2000 lx. After 60 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that each explant had an average of 4.06 adventit...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com