Rooting culture medium for culturing dendrobium officinale plantlets

A rooting medium and technology of Dendrobium candidum, applied in the field of plant tissue culture, can solve problems such as difficulty in popularization and application, unreasonable formula of rooting medium, failure to guarantee survival rate, etc., and achieve the effect of improving reproduction and promoting industrial production

Active Publication Date: 2015-03-25
ANHUI TONGCHUANG MODERN AGRI INVESTMENT DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above two applications: the technical scheme disclosed in Chinese Patent Application No. 201310173395.0 is to sow the pollen of mature Dendrobium officinale seeds on the above-mentioned medium, after 3 months the mother seedlings will grow out, and then the seedlings will be artificially inoculated. In actual application, the survival rate cannot be guaranteed, and it is difficult to promote and apply
The technical scheme disclosed in Chinese Patent Application No. 201210102034.2 mainly adopts hormone-free tissue culture technology, but the formulation of its rooting medium is not reasonable enough and needs further improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A rooting medium for the cultivation of Dendrobium officinale tissue culture seedlings of the present embodiment, the rooting medium uses self-made mother liquor as the basic medium, adds agar 6g / L in the basic medium, white sugar 20g / L, gac 0.5g / L L, banana 50g / L, self-made natural organic liquid fertilizer 1.5g / L, sterilant 2.5ml / L. The preparation process of the rooting medium in the present embodiment is specifically as follows: (1), each component is weighed according to the composition and dosage of self-made mother liquor, makes it fully dissolve, and takes in order according to corresponding concentration. (2), add various substances as required in the self-made mother liquor, which are respectively: agar 6g / L, white sugar 20g / L, activated carbon 0.5g / L, banana 50g / L, self-made natural organic liquid fertilizer 1.5g / L , sterilizing agent 2.5ml / L, wherein: the banana in the present embodiment refers to banana puree, and this banana puree is that banana is peeled,...

Embodiment 2

[0031] A kind of rooting culture medium of Dendrobium officinale tissue culture seedling of the present embodiment is used, and rooting culture medium uses self-made mother liquor as basic culture medium, adds agar 5g / L in basic culture medium, white sugar 20g / L, gac 0.5g / L , banana 53g / L, self-made natural organic liquid fertilizer 1.6g / L. The rooting medium of this embodiment was used for cultivation, and the specific steps of the cultivation method of Dendrobium candidum tissue-cultured seedlings were the same as in Example 1, and the specific conditions of the cultured Dendrobium candidum tissue-cultured seedlings were shown in Table 1.

Embodiment 3

[0033] A rooting medium for the cultivation of Dendrobium officinale tissue culture seedlings of the present embodiment, the rooting medium uses self-made mother liquor as the basic medium, adds agar 9g / L in the basic medium, white sugar 22g / L, gac 0.52g / L , banana 55g / L, self-made natural organic liquid fertilizer 1.5g / L, sterilizer 1.5ml / L. The rooting medium of this embodiment was used for cultivation, and the specific steps of the cultivation method of Dendrobium candidum tissue-cultured seedlings were the same as in Example 1, and the specific conditions of the cultured Dendrobium candidum tissue-cultured seedlings were shown in Table 1.

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PUM

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Abstract

The invention discloses a rooting culture medium for culturing dendrobium officinale plantlets and belonging to the field of the plant tissue culturing technology. The rooting culture medium for culturing the dendrobium officinale plantlets uses a self-made mother liquor as a basic culture medium; 5-9 g/L of agar, 20-22 g/L of sugar, 0.5-0.55 g/L of active carbon, 50-55 g/L of banana, 1.5-1.8 g/L of self-made natural organic liquid fertilizer and 1.5-2.5 ml/L of sterilizing agent are added to the basic culture medium; and the pH value in the rooting culture medium is 5.4-5.8. The rooting culture medium plays a role in promoting roots well and solves problems of slow rooting and lower survival rate in the traditional culturing method of dendrobium officinale; compared with the seedlings cultured by the culture mediums without natural organic matters, the thickness, height and leaf quantity of the cultured dendrobium officinale seedlings are increased and the roots are promoted well.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a rooting medium for dendrobium officinale tissue culture seedling culture. Background technique [0002] Dendrobium candidum is a perennial herb belonging to epiphytic orchids. It is commonly known as Tiepi Fengdou. Because of its black outer stems, it is also known as black knot grass. Dendrobium is a commonly used precious traditional Chinese medicine with a long history of application. In "Shen Nong's Herbal Classic" It has been listed as top grade, and it has been proven that it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, and prolonging life. However, due to the special growth environment and limited...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑毓华于太和周道阳
Owner ANHUI TONGCHUANG MODERN AGRI INVESTMENT DEV
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