A kind of rooting medium for dendrobium officinale tissue culture seedling cultivation

Abstract

The invention discloses a rooting culture medium for culturing dendrobium officinale plantlets and belonging to the field of the plant tissue culturing technology. The rooting culture medium for culturing the dendrobium officinale plantlets uses a self-made mother liquor as a basic culture medium; 5-9 g / L of agar, 20-22 g / L of sugar, 0.5-0.55 g / L of active carbon, 50-55 g / L of banana, 1.5-1.8 g / L of self-made natural organic liquid fertilizer and 1.5-2.5 ml / L of sterilizing agent are added to the basic culture medium; and the pH value in the rooting culture medium is 5.4-5.8. The rooting culture medium plays a role in promoting roots well and solves problems of slow rooting and lower survival rate in the traditional culturing method of dendrobium officinale; compared with the seedlings cultured by the culture mediums without natural organic matters, the thickness, height and leaf quantity of the cultured dendrobium officinale seedlings are increased and the roots are promoted well.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a rooting medium for dendrobium officinale tissue culture seedling culture. Background technique [0002] Dendrobium candidum is a perennial herb belonging to epiphytic orchids. It is commonly known as Tiepi Fengdou. Because of its black outer stems, it is also known as black knot grass. Dendrobium is a commonly used precious traditional Chinese medicine with a long history of application. In "Shen Nong's Herbal Classic" It has been listed as top grade, and it has been proven that it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, and prolonging life. However, due to the special growth environment and limited...

AI Technical Summary

Problems solved by technology

In the above two applications: the technical scheme disclosed in Chinese Patent Application No. 201310173395.0 is to sow the pollen of mature Dendrobium officinale seeds on the above-mentioned medium, after 3 months the mother seedlings will grow out, and then the seedlings will be artificially inoculated. In actual application, the survival rate cannot be guaranteed, and it is difficult to promote and apply

The technical scheme disclosed in Chinese Patent Application No. 201210102034.2 mainly adopts hormone-free tissue culture technology, but the formulation of its rooting medium is not reasonable enough and needs further improvement