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Culture medium for culturing hoya tissue and culture method

A tissue culture and culture medium technology, which is applied in the field of plant tissue culture, can solve the problems of low survival rate, poor use effect, and restriction of the development of the propagation technology of Orchid spp. The effect of high quality, germination and rooting rate guarantee

Inactive Publication Date: 2014-06-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And when adopting existing ball blue tissue culture medium formula to carry out tissue culture, the survival rate of ball seedling of tissue culture bottle seedling is low, and use effect is poor, has limited the further development of ball blue propagation technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Medium formula:

[0023] Explant induction medium: agar 7g L -1 +basic medium MS+kinetin KT 0.5mg·L -1 +6-Benzylpurine 6-BA 0.5mg·L -1 +NAA 0.5mg·L -1 ;

[0024] Proliferation subculture medium: agar 7g L -1 +basic medium MS+6-benzylpurine 6-BA 1.5mg·L -1 +NAA 0.2mg·L -1 +Gibberellin GA 0.5mg·L -1 +cerium nitrate Ce(NO 3 ) 3 0.20mg·L -1 ;

[0025] Bud differentiation medium: agar 7g L -1 +basic medium MS+kinetin KT 0.5mg·L -1 +NAA 0.5mg·L -1 +Gibberellin GA 1.0mg·L -1 + Lanthanum nitrate La(NO 3 ) 3 0.15mg·L -1 +cerium nitrate Ce(NO 3 ) 3 0.20mg·L -1 ;

[0026] Rooting medium: agar 7g L -1 +basic medium White+gibberellin GA 0.5mg·L -1 + Lanthanum Chloride LaCl 3 10mg·L -1 ;

[0027] Rooting soaking solution, including naphthaleneacetic acid NAA 0.2mg·L -1 , Gibberellin GA 0.5mg·L -1 , cerium nitrate Ce(NO 3 ) 3 0.15mg·L -1 + Lanthanum Chloride LaCl 3 15mg·L -1 .

[0028] The method of using the above medium for tissue culture is as fo...

Embodiment 2

[0044] Medium formula:

[0045] Explant induction medium: agar 10g L -1 +Basic Medium MS+Kinetin KT 1.0mg·L -1 +6-Benzylpurine 6-BA 1.0mg·L -1 +Naphthylacetic acid NAA 1.0mg·L -1 ;

[0046] Proliferation subculture medium: agar 10g L -1 +basic medium MS+6-benzylpurine 6-BA 2.0mg·L -1 +NAA 0.2mg·L -1 +Gibberellin GA 0.8mg·L -1 +cerium nitrate Ce(NO 3 ) 3 0.30mg·L -1 ;

[0047] Bud differentiation medium: agar 10g L -1 +Basic Medium MS+Kinetin KT 1.0mg·L -1 +NAA 0.5mg·L -1 +Gibberellin GA 1.0mg·L -1 + Lanthanum nitrate La(NO 3 ) 3 0.20mg·L -1 +cerium nitrate Ce(NO 3 ) 3 0.30mg·L -1 ;

[0048] Rooting medium: agar 10g L -1 +basic medium White+gibberellin GA 1.0mg·L -1 + Lanthanum Chloride LaCl 3 20mg·L -1 ;

[0049] Rooting soaking solution, including naphthaleneacetic acid NAA 1.0mg·L -1 , Gibberellin GA 0.5mg·L -1 , cerium nitrate Ce(NO 3 ) 3 0.25mg·L -1 + Lanthanum Chloride LaCl 3 30mg·L -1 .

[0050] The method of using the above medium ...

Embodiment 3

[0066] Medium formula:

[0067] Explant induction medium: agar 6g L -1 +Basic Medium MS+Kinetin KT 0.80mg·L -1 +6-Benzylpurine 6-BA 0.8mg·L -1 +NAA 0.75mg·L -1 ;

[0068] Proliferation subculture medium: agar 6g L -1 +basic medium MS+6-benzylpurine 6-BA 1.7mg·L -1 +NAA 0.2mg·L -1 +Gibberellin GA 0.6mg·L -1 +cerium nitrate Ce(NO 3 ) 3 0.25mg·L -1 ;

[0069] Bud differentiation medium: agar 6g L -1 +Basic Medium MS+Kinetin KT 0.7mg·L -1 +NAA 0.5mg·L -1 +Gibberellin GA 1.0mg·L -1 + Lanthanum nitrate La(NO 3 ) 3 0.18mg·L -1 +cerium nitrate Ce(NO 3 ) 3 0.25mg·L -1 ;

[0070] Rooting medium: agar 6g L -1 +basic medium White+gibberellin GA 0.75mg·L -1 + Lanthanum Chloride LaCl 3 15mg·L -1 ;

[0071] Rooting soaking solution, including naphthaleneacetic acid NAA 0.6mg·L -1 , Gibberellin GA 0.5mg·L -1 , cerium nitrate Ce(NO 3 ) 3 0.20mg·L -1 + Lanthanum Chloride LaCl 3 23mg·L -1 .

[0072] The method of using the above medium for tissue culture is...

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Abstract

The invention discloses a culture medium for culturing hoya tissue and a culture method. The culture medium comprises an explant induction culture medium, a proliferation subculture culture medium, a bud differentiation culture medium, a rooting culture medium and a rooting soak solution, wherein the explant induction culture medium is composed of agar, minimal culture medium MS, kinetin KT, 6-benzyladenine 6-BA and naphthylacetic acid NAA; the proliferation subculture culture medium is composed of the agar, the minimal culture medium MS, the 6-benzyladenine 6-BA, the naphthylacetic acid NAA, gibberellin GA and cerous nitrate; the bud differentiation culture medium is composed of the agar, the minimal culture medium MS, the kinetin KT, the naphthylacetic acid NAA, the gibberellin GA, lanthanum nitrate La(NO3)3 and the cerous nitrate Ce(NO3)3; and the rooting culture medium is composed of the agar, minimal culture medium White, the gibberellin GA and lanthanum chloride LaCl3. By utilizing the culture medium, the quality of tissue culture seedling of hoya is greatly improved, and propagation of the tissue culture seedling of the hoya is effectively improved so that industrial production of the hoya is further promoted.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium and a culture method for plant tissue culture. Background technique [0002] With the development of plant tissue culture technology, it has become a trend to carry out commercial batch cultivation and production of ball orchids through tissue culture technology. Traditional ball orchids are almost always cultivated by cutting propagation method, but this propagation method takes root slowly, and after survival and transplantation, the seedlings are not vigorous and the growth is relatively slow. Therefore, it is an important technical means to solve the above breeding problems by tissue culture technology to propagate the bulbous orchid. At present, the tissue culture technology of ball blue has been reported in the laboratory stage experiment, and there is a precedent of establishing a sterile line and being able to multiply. A means ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 叶萌唐敏段晓宇汪维双
Owner SICHUAN AGRI UNIV
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