67 results about "Root cap" patented technology

The invention belongs to the field of plant biotechnologies and particularly discloses a culture medium and culture method for promoting the growth of regeneration buds of echinacea purpurea. According to the culture medium, DA-6 (diethyl aminoethyl hexanoate) of corresponding concentration is particularly added to an MS formula in accordance with that the regeneration buds of different ploidies have different sensitivities to DA-6, besides adding 15-60g/L of saccharose, 3-9g/L of agar and 0.01-0.2mg/L of NAA (naphthalene acetic acid). The culture medium has the advantage that the height, weight, the weight, diameter and number of roots, the length of primary roots, total root length, root cap ratio and transplanting survival rate of the regeneration buds all can be increased remarkably. By applying the culture medium and the culture method, the development of the scientific research work related to the biotechnological breeding of echinacea purpurea can be promoted.

The invention discloses a method for improving chromium resistance and cadmium resistance of Festuca arundinace with compost fermentation liquid, which comprises the following steps: adding 200g of oven-dried soil into disposable plastic cups of a diameter 7cm and seeding 0.8g of Festuca arundinace seeds, respectively adding microbial inoculums to lawn plants after seeding for 10 days, respectively adding cadmium chloride solution and potassium dichromate solution to soil, repeating the experiment for three times (60 samples in all), continuing culturing at room temperature while feeding water at a fixed amount every day during the experiment to ensure uniform environment among treatment chambers, and measuring indicators after growing for 30 days. The experimental results shows that after the Bacillus thuringiensis preparation is added, the underground dry weight and the root-top ratio of Festuca arundinace are increased by 137.5% and 102.9% respectively compared with the underground dry weight and the root-top ratio of the control, and the lawn plant growth is promoted, and the chromium resistance and cadmium resistance of Festuca arundinace are remarkably improved.

The invention discloses a peach tree green stock-green branch cut grafting rapid seedling growing method. A half-lignification green branch is used as a scion to be grafted to a green stock of a nutrition bag in a cut grafting mode, leaves below the grafted port of the green stock are kept, the grafted port and the scion are bound by plastic strips in a sealed mode, only bud eyes are exposed, as the lignification degree of the green stock and the scion is low, tissue cells are active, the leaves left on the green stock can continue to conduct photosynthesis to manufacture nutrient substances which are supplied to calluses, coalescence of the grafted port is achieved rapidly in a growing period, and therefore the rate of survival of grafting of the lignification scion in a resting period is improved by 20%-30% compared with annual rootstock seedlings . Grafted seedlings can be conveyed out of a flower nursery for garden building from August to September, the seedlings of the nutrition bag do not need pruning, leaf cutting and watering, the root caps are not damaged after the seedlings are planted, a seedling buffering period is avoided after planting, and the leaves fall down and dormancy occurs after the seedlings sprout and slightly mature. The rate of survival of planting of garden building is high, the seedlings grow rapidly, fructification is achieved early, the yield is high, and the peach tree green stock-green branch cut grafting rapid seedling growing method is particularly suitable for garden building of mountainous regions where are dry and have no irrigation conditions in winter and spring.

The invention discloses a method of using a three dimensional reconstruction imaging technology to analyze the structure of root tip cells of arabidopis thaliana, and belongs to the field of cell structure analysis. According to the method, roots of arabidopis thaliana are subjected to a horizontal freezing slicing treatment to obtain root cross sections, which only contain the root caps, the thickness of the slice is only 10 [mu]m, and thus the influence of the resolution ratio of the Z axis of a microscope on the imaging reality is greatly reduced; by optimizing the conditions of microscopic photography, the shooting stepping is minimized to less than 0.4 [mu]m, the Pinhole value is reduced so as to ensure that the thickness of a calculated optical slice is less than 0.8 [mu]m, the fineness of the photos is guaranteed; the scanning range comprises the whole structure of a stele cell from top to bottom; a finer microscopic imaging picture can be obtained, the influence of stray light of non-focal planes on the images is effectively reduced, the image resolution ratio is improved, the structure of a studied object can be realistically represented; and by using the provided three dimensional reconstruction imaging technology, the intra-cell endoplasmic reticulum structure can be determined more directly and accurately.

The embodiment of the invention provides a plant root cap integrated monitoring system and a method thereof. The system comprises a data acquisition device and a data processing control device. The data acquisition device comprises a cap data acquisition device and a root data acquisition device. The cap data acquisition device is arranged on the periphery of the cap of plants to be monitored and is used for acquiring cap data of the plants to be monitored. The root data acquisition device comprises minirhizotron and a root data acquisition module arranged inside the minirhizotron. The minirhizotron is arranged on the periphery of the plants to be monitored and is used for acquiring root data of the plants to be monitored. The data processing control device processes received cap data and root data and acquires a root cap data set of a same plant at the same time. The method is an application method of the system. According to the embodiment of the invention, plant root cap monitoring accuracy is raised, and plant root cap data synchronous acquisition and root cap monitoring integration are realized.

A novel method of raising seedlings by cutting with electromagnetic pulse for Taxus media is the method of raising the seedlings by cutting through the utilization of the electromagnetic pulse for assisting. The method is realized by the following technological processes: preparing a seedbed-burying a cathode screen and paving matrix-arranging an anode screen-clipping and cutting cutting slips-processing by an electric field- hardening seedling and transplanting, wherein in the electric field action environment, the phenomena of maturing and browning callus are restrained, and the formation time of root primordial is shortened to grow roots in 25 days; and under the condition of free of the electric field action environment, due to the maturing of the callus, the root primordium can break through the epidermis in about 45 days; and additionally, as the photosynthetic efficiency is improved, the number of root caps is obviously improved compared with that of the roots, so as to facilitate the survival of strong seedlings and transplanting. Compared with the prior art, both the rooting rate of cutting and the survival rate of transplanting reach to more than 95%.

The invention relates to the field of plant culture, in particular to a plant seedling growing method and a device thereof. The device special for plant root casing pipe seedling growing is provided with a casing pipe made of plastic, resin, rubber, paper or other thin substances or degradable composite materials. Through the casing pipe, roots of planted seedlings are limited within the casing pipe, curling or extending to grow with the pipe wall as the boundary, a protective layer is formed from pipe wall film, and during carrying, film tearing, transplanting, field planting and the like, roots or root caps or root hairs of seedlings are not injured; meanwhile, the casing pipe after transplanting is conveniently recycled, resources are saved, the seedlings are conveniently transplanted through a tearing line, the survival rate of seedling transplanting is increased, and the growth cycle of the seedlings is shortened.

The invention discloses a method for increasing the watermelon grafting survival rate. According to the method, abscisic acid solution is sprayed to watermelon seedlings and rootstock seedlings before grafting, and small sheds which can preserve heat and humidity and is subjected to bactericide treatment are set up in advance; grafting is conducted through the root-cutting cleft grafting method, and bactericide is sprayed after grafting is completed. The plant stress resistance is improved, the grafting method is efficient, the suitable environment is beneficial to fusion of the watermelon seedlings and the rootstock seedlings, the high survival rate and strong seedlings are guaranteed, seedling recovering is quick after the watermelon seedlings are transplanted, and a basis is laid for achieving high yield and high quality. Test results show that by adopting the method, the grafting survival rate reaches 98.7%, and the repeat deviation is small; compared with conventionally grafted seedlings, the stem diameter, the maximum leaf area, the root cap ratio, the chlorophyll content and the root vigor are increased.

The invention discloses a promoter miR390 and applications thereof. The promoter is a deoxyribonucleic acid (DNA) fragment shown as in (1), (2) or (3) as follows: (1) a DNA fragment shown in a sequence 1 in a sequence table; (2) a DNA fragment which is hybridized with a DNA sequence limited by the DNA fragment shown in the sequence 1 in the sequence table under strict conditions and has same functions as the DNA sequence; and (3) a DNA fragment which has at least 90 percent of homology with the DNA sequence limited by the DNA fragment shown in the sequence 1 in the sequence table and has the same functions as the DNA sequence. Proved by experiments, the miR390 DNA fragment disclosed by the invention is a promoter, and moreover, the promoter has very strong specific expression activity, and is specifically expressed at the root cap of a plant. The promoter disclosed by the invention is capable of providing a facilitating device for function researches of specifically expressed genes and transgenic breeding of plants and has an important meaning.

The invention provides a cratevareligiosa G. Forster root cutting seedling culture method which includes the steps: (1) selecting seminal roots according to germination ability and richness of seminalroot sources; (2) cutting and disinfecting the seminal roots; (3) controlling illumination, moisture and temperature of root cuttings and matching substrates; (4) controlling hormone treating kinds,hormone treating concentration and hormone treating time; (5) selecting a cutting mode and managing the moisture after planting; (6) dividing roots and forming seedlings. According to the method, rootsections with fibrous roots of the edges of cratevareligiosa G. Forster root caps are selected as root cutting seminal roots, seed tree cap shapes are not affected, the method is simple and convenient to operate, a propagation coefficient is high, the method optimizes the thickness of seminal roots suit suitable for the root cuttings, cutting modes, matching of root cutting substrates, hormone kinds, hormone concentration and hormone treating time, bud germination time is shortened, bud germination rate, rooting rate and seedling survival rate are effectively increased, cratevareligiosa G. Forster seedlings are rapidly cultured in a short time, and the foundation is laid for development and protection of cratevareligiosa G. Forster resources.

The invention discloses a method for purifying a water body by planting submerged plants with different root-shoot ratios together. According to the method, at least two submerge plants with different root-shoot ratios are used. The method comprises the following steps: (1) choosing a water body to be purified, and flattening and clearing the substrate of the water body; (2) manufacturing field-planting grids; (3) choosing cultured submerge plants with different root-shoot ratios, and screening and trimming the submerge plants; (4) determining the planting positions for the submerge plants with different root-shoot ratios; (5) transplanting the submerge plants. By planting different submerge plants with different root-shoot ratios, the nutritive salt loads of water body and precipitates can be reduced, moreover, the different submerge plants can inhibit each other, the excess growth can be avoided, thus the whole water ecological system will not be influenced by the excessive submerge plants, and the method can finally purify the water body.

The invention belongs to the technical field of soil conditioners, and particularly relates to a soil conditioner for promoting acer truncatum root growth and preparation and application thereof. Thesoil conditioner for promoting acer truncatum root growth comprises biochar and bacterial manure, wherein the mass ratio of the biochar to the bacterial manure is 20-35:20-30. The soil conditioner provided by the invention not only can promote acer truncatum root growth , but also can significantly improve the growth amount of acer truncatum seedling plant height and stem diameter, improve the root-crown ratio and promote the growth and development of root system forms.

There is provided a hydroponics device including a cover forming an upper part of a cultivation planter and a cover support supporting the cover from beneath the cover and detachably connected to a pan. The cover supports plants, which are a predetermined number of different strains of the same species, so that tips (portions respectively including rootcaps and apical meristems) of taproots of the plants are immersed in a nutrient solution in the pan.

The invention discloses a tea tree root stock grafting and seedling raising method. The method comprises the steps of stock selection and preparation, stock preparation, camellia variety matching, scion selection, scion elimination, root stock grafting opening cutting, grafting treatment and grafting management. For stock selection, tea trees which grow robustly, are free of diseases and insect pests and bear few fruits are selected, the best tree form is a single stem, the ground diameter is 5-10 cm, and the best trunk branches are 1 cm or above; for stock preparation, trunks and branches, 0.8-1m away from the ground, of stocks are cut off, and tea seedling roots with root crowns removed are used as the stocks, so that germination and growth of adventitious buds of tea tree root crowns are avoided, a large amount of sprout removal work in the tea tree seedling raising process is reduced, and the defects that tea tree short-spike cutting seedlings have no main roots, and tea seedlings are poor in drought and cold resistance are overcome; and in addition, the labor intensity of grafting, replanting and plant switching in an old tea garden is reduced, the grafting efficiency of tea trees is improved, and the purity of the seedlings is improved.