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Novel method of raising seedlings by cutting with electromagnetic pulse for Taxus media

A Mandia yew, electromagnetic pulse technology, applied in horticultural methods, botanical equipment and methods, agricultural machinery and equipment, etc., can solve the problems of low rooting rate, low value-added coefficient, difficult organ differentiation, etc., and achieve root The effect of increasing the ratio of shoots and the number of roots, promoting rapid growth, and enhancing stress resistance

Inactive Publication Date: 2013-09-18
HUNAN ACAD OF FORESTRY
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Problems solved by technology

From the first related literature report in 2004 to now, the main problems in the tissue culture of Taxus mandia are: it is difficult for the callus to undergo organ differentiation after subculture, the value-added coefficient is not high, and the rooting rate is not high. Among them, the highest is 27.78% inside the bottle, and the highest is 58.33% outside the bottle

Method used

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Embodiment Construction

[0017] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the following examples are used to describe the present invention in detail.

[0018] The new method of electromagnetic pulse cutting seedling cultivation of Taxus Mandia is realized through the process of seedbed preparation→cathode net burying and substrate laying→anode net erection→cutting pruning and cutting→electric field treatment→seeding hardening and transplanting.

[0019] First, use bricks and cement to build a seedbed with a height of 15cm and a width of 1.2m. Several such seedbeds can be built in the greenhouse according to the needs, in a vertical and horizontal layout, and the beds and walkways are all smoothed with cement; First lay a layer of 5cm thick pebbles on the bottom of the bed as a drainage layer, then lay a cathode net of an electric field net horizontally, then spread a layer of carbon powder about 3-5cm thick on the m...

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Abstract

A novel method of raising seedlings by cutting with electromagnetic pulse for Taxus media is the method of raising the seedlings by cutting through the utilization of the electromagnetic pulse for assisting. The method is realized by the following technological processes: preparing a seedbed-burying a cathode screen and paving matrix-arranging an anode screen-clipping and cutting cutting slips-processing by an electric field- hardening seedling and transplanting, wherein in the electric field action environment, the phenomena of maturing and browning callus are restrained, and the formation time of root primordial is shortened to grow roots in 25 days; and under the condition of free of the electric field action environment, due to the maturing of the callus, the root primordium can break through the epidermis in about 45 days; and additionally, as the photosynthetic efficiency is improved, the number of root caps is obviously improved compared with that of the roots, so as to facilitate the survival of strong seedlings and transplanting. Compared with the prior art, both the rooting rate of cutting and the survival rate of transplanting reach to more than 95%.

Description

technical field [0001] The invention relates to the technical field of forestry, in particular to a method for using electromagnetic pulses to assist cutting seedling cultivation. Background technique [0002] Taxus Mandia is currently the fastest growing yew hybrid variety with the highest paclitaxel content in the world. Its growth rate and biomass are 3 to 5 times that of other yew varieties, and the paclitaxel content is 4 to 10 times that of other varieties. It has strong adaptability, wide application range, strong resistance to diseases and insect pests, and can withstand low temperatures of -25°C. It can grow well on slightly acidic and neutral soils with an altitude of 200 to 2000, and has a fast growth rate and annual growth. The volume can reach 50-80cm. Taxus Mandia has high paclitaxel content, and the content of each part of the whole plant is higher than that of other varieties of Taxus. Its comprehensive utilization value is very significant, and it is calle...

Claims

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Application Information

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IPC IPC(8): A01G7/04A01G1/00
CPCY02P60/14
Inventor 童方平李贵刘振华陈瑞杨莉邝鼎
Owner HUNAN ACAD OF FORESTRY
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