100 results about "Plant stem cell" patented technology

The invention relates to the technical field of cosmetics, in particular to a natural plant extract composition and application thereof in the cosmetics. The natural plant extract composition is prepared from the following raw materials in parts by weight: 0.1 to 10 parts of plant stem cells, 2 to 10 parts of grapefruit extracts, 1 to 10 parts of radix asparagi extracting solution, 1 to 10 parts of gingko extracts, 5 to 10 parts of lucid ganoderma extracting solution, and 1 to 5 parts of burdock extracting solution. The natural plant extract composition provided by the invention not only has remarkable moisturizing and anti-aging effect, but also has the effects in removing acnes, adjusting grease secretion, resisting inflammation and relieving, and can be used for preparing moisturizing, anti-aging or acne-removing cosmetics.

The invention discloses a stem cell tissue culture seedling raising technique. The technique comprises the steps of 1, taking a fresh plant, conducting sterilization on the surface and cutting the plant; 2, conducting tissue differentiation and reserving a cambium; 3, culturing the cambium on an isolation medium. According to the technique, existing methods are improved based on a traditional tissue culture technique, plant stem cells are taken as a target, induction, separation and explant culture are conducted, and a corresponding stem cell culture system is established. By the adoption of the plant stem cell culture technique, the plant culture technique is enriched, and a novel research direction and opportunity is provided for commercialized production of natural products and development of the plant biotechnology.

The invention relates to the technical field of plant stem cell culture, particularly an apple stem cell culture method and apple stem cells cultured by the method. The method comprises the following steps: by using an apple new branch as a raw material, inducing to form callus, and carrying out enrichment culture and amplification culture to obtain the apple stem cells. The method provided by the invention has the advantages of shorter period and high yield. The method can obtain abundant apple stem cells within about 48 days. The induction culture medium used in the apple stem cell culture process and the variety of the phytohormones contained in the enrichment culture medium are proper and suitable in proportion, and can ensure fast dedifferentiation and abundant reproduction of apple branch cells. The apple stem cells can be freeze-dried or extracted and used for preparing food, drugs, health products or cosmetics. The apple stem cell extraction method provided by the invention is simple and easy to implement and has higher extraction efficiency. The prepared extract contains abundant flavones and polyphenols.

The invention discloses an anti-ageing skincare product. The anti-ageing skincare product comprises 0.001 to 3 weight percent of a plant stem cell active matter and 0.01 to 3 weight percent of Dragosine carnosine, wherein the plant stem cell active matter is at least one of a Phytocelltec<TM> Malus Domestica apple stem cell, a Phytocelltec <TM> Solar Vitis grape stem cell, a Phytocelltec <TM> Alp Rose rose leave stem cell, a Phytocelltec <TM> Argan nut stem cell and a Regenistem Rice Ap red rice stem cell. The preparation method of the anti-ageing skincare product comprises a step of adding the plant stem cell active matter and the Dragosine carnosine into a skincare product. The anti-ageing skincare product comprises the plant stem cell active matter and the Dragosine carnosine at the same time, and under the synergistic action of the two components, the anti-ageing skincare product can obviously improve skin quality, skin elasticity and proliferative capacity of skin, and the effect is obviously higher than that of a skincare product in which the plant stem cell active matter or the Dragosine carnosine is added singly.

The invention discloses an anti-aging cosmetic skin care product, which contains 0.001-3wt% of plant stem cell actives and 0.01-3wt% of Dragosine carnosine. The plant stem cell actives are PhytocelltecTM MalusDomestica apple stem cells, PhytocelltecTMSolarVitis grape stem cells, PhytocelltecTMAlpRose rose leaf stem cells, PhytocelltecTMArgan At least one of nut stem cells and RegenistemRiceAp red rice stem cells, and its preparation method: adding plant stem cell active substances and Dragosine carnosine to beauty and skin care products. The product of the present invention contains plant stem cell active substance and Dragosine carnosine at the same time. Under the synergistic effect of these two components, the product can obviously improve skin quality, improve skin elasticity, and improve skin proliferation ability, and the effect is obviously higher than adding plant stem cell active substance alone or Dragosine Carnosine.

The invention discloses an induction and in-vitro culture method of plant stem cells derived from apical meristem of catharanthus roseus roots. The induction method comprises the following steps: cutting off a root cap from the root tip of the catharanthus roseus, performing inoculating to an induction culture medium, performing culturing in dark, and proliferating and growing a stem cell layer inan explant to form a cell cluster; transferring the layered stem cell layer to a subculture medium for culturing for 10-15 days, and performing complete culturing in dark; and performing transferringto a new subculture medium, and performing culturing for 10-15 days under the same condition to obtain a catharanthus roseus stem cell line with stable cell morphology. A catharanthus roseus stem cell culture system is established by inducing apical meristem of the catharanthus roseus roots, stable subculture can be performed under certain conditions, and epigenetic variation is avoided. The catharanthus roseus stem cell culture system established by the invention has the characteristics of being high in growth speed, high in genetic stability and the like, and culture with a 10L bioreactor is preliminarily realized.

The present invention relates to a stem cell derived from cambium of family Gingkoaceae and to method for the isolated culturing thereof. The stem cell derived from cambium of family Gingkoaceae according to the present invention is advantageous as it is isolated at a non-differentiated state without passing through a dedifferentiation process and stably maintained without causing a variation in the cellular growth rate and growth pattern even during a long-term culture, thereby enabling a mass culture. In addition, the stem cell derived from cambium of family Gingkoaceae according to the present invention showed an antioxidant effect similar to or higher than that of existing synthetic antioxidants when the radical-scavenging activity for scavenging radicals generated by the treatment of an oxidant is measured, and thus can be valuably used as an excellent anti-inflammatory composition.

The invention provides plant extracts which are prepared by the following method: (1) cultivating plant cells in vivo and collecting the culture; (2) crushing the culture obtained in the step (1) and collecting a supernatant; and (3) colleting components with a molecular weight of less than 4500Da (preferably less than 3500Da, and most preferably less than 3000Da) in the supernatant, and optionally freezing and drying. The invention further provides foods or health products containing the plant extracts, and an application thereof.

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof. The cell line, the lysate, the extract, and the culture medium has minimized side effects compared to the conventional therapeutic drugs, is safe to the human body, is involved directly in the growth of cancer to induce cancer cell death, and shows anticancer activity of inhibiting angiogenesis occurring in carcinogenesis. Accordingly, the cell line, the lysate, the extract and the culture medium is useful for the prevention, treatment and alleviation of cancer.

The present invention relates to a cell line derived from the quiescent center of a plant and a method for isolating the same, more specifically, relates to a quiescent center-derived homogeneous cell line of single-cell origin, which is obtained from the quiescent center of a plant without needing a separate de-differentiation process, and a method for isolating the same.

The invention provides a milky mask based on viable yeast and plant stem cells. The milky mask is prepared from yeast extract, plant stem cell extract, nano particles, a conditioner, a thickening agent and an assistant and is specifically prepared from lactic acid bacillus / eriodictyon californicum fermentation product extract, bifida ferment dissolved cell extract, beer saccharomycete extract, grape fruit cell extract, chondrus crispus extract, centella extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea extract, glycyrrhiza glabra root extract, chamomilla recutita flower extract, rosemary leaf extract, purslane herb extract and nanometer titania. The mask has the effects of whitening skin and preserving moisture, restoring skin and delaying senescence, is free of irritation, is easy to absorb, enables the skin to be fresh and cool, tighter, finer and smoother, and is suitable for being used for people with skin sensitivity.

The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. Accordingly, the cell line derived from the cambium of an herbaceous plant having a storage root makes it possible to produce large amounts of useful plants which are difficult to cultivate outdoor due to various problems associated with the period of cultivation, the selection of cultivation land, cultivation cost and the like.

The present invention relates to a plant stem cell derived from the cambium of family Solanaceae, and to a method for isolating and culturing the same. A stem cell derived from the cambium of family Solanaceae, according to the present invention, is useful as it can be isolated in an undifferentiated state without undergoing a dedifferentiation process and stably maintained without causing any variation in the cell growth rate and growth pattern even during long-term culture, thereby enabling large-scale culture. Moreover, the stem cell derived from the cambium of family Solanaceae and the culture thereof, according to the present invention, are useful for preventing and delaying aging as they have the effects of inducing biosynthesis of procollagen and more efficiently inhibiting the generation of enzymes related to aging than retinoic acid, which is known as having an excellent effect in skin wrinkle improvement.

The present invention relates to an anti-aging or antioxidant composition which contains, as an active ingredient, a plant stem cell line derived from the cambium of Panax ginseng, including wild ginseng and ginseng, or an extract thereof, a lysate thereof and a culture thereof. The composition has minimized side effects compared to existing anti-aging agents and antioxidants, and thus is safe for the skin. Also, the composition of the present invention shows an antioxidant effect of inhibiting reactive oxygen species caused by exposure to UV radiation that is the major cause of skin aging, and it can effectively reduce or inhibit aging-related factors. Thus, the composition is useful for the prevention and inhibition of aging.

The present invention relates to a composition for enhancing immunity containing a cell line derived from the cambium of Panax ginseng including wild ginseng or ginseng, or the grounded substance, extract, or culture medium of the cell line as an active ingredient. The cell line and ground substance, extract, and culture medium of the cell line according to the present invention compose a natural product-derived composition. The cell line, ground substance, extract, and culture medium are safe to a human body by minimizing side effects of a conventional immunity booster, and also effectively increase activity of NK cells charging innate immunity. As well, the cell line, ground substance, extract, and culture medium appear to enhance acquired immunity by increasing proliferation rate of lymph node cells when re-exposing the cells to an antigen in a specific immune reaction, thus are useful as an immunity booster. Especially, the cell line, ground substance, extract, and culture medium also effectively increase the number of bone marrow cells, thus are not only used as an immune reaction adjuvant, but also used in preventing and treating anemia caused by hematopoiesis.

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Panax ginseng cambium-derived cell line including wild ginseng or ginseng; a lysate thereof; an extract thereof; or a culture medium thereof.The cell line according to the present invention, a lysate thereof, an extract thereof and a culture medium thereof are derived from a natural and have minimized side effects compared to the conventional therapeutic drugs, and thus are safe for the human body. Also, they are involved directly in the growth of cancer to induce cancer cell death effectively, and show anticancer activity of inhibiting or reducing the formation of tumor or the growth of tumor, Accordingly, the cell line, the lysate thereof, the extract thereof and the culture medium thereof are useful for the prevention, treatment and alleviation of cancer.

The invention belongs to the technical field of anti-aging and moisturizing cosmetics, and particularly relates to a stem cell complex factor anti-aging moisturizing cosmetic which consists of the following raw materials: mixed fresh petals, mint leaves, mulberry leaves, mixed plant stem cell active matter, xanthan gum, phospholipid, protamine, deionized water, carbomer, benzyl alcohol and cetostearyl alcohol. The preparation method of the stem cell complex factor anti-aging moisturizing cosmetic comprises the following steps: S1, uniformly spreading mixed fresh petals, mint leaves and mulberry leaves on a grid plate of a distillation device, then pouring deionized water into distillation boxes of the distillation device, and switching on a power supply for distillation to obtain a mixtureof hydrolat and essential oil for later use. Through arrangement of the multiple layers of distillation boxes, petals can be fully spread, the heating area of mixed fresh petals is increased, and distillation efficiency is improved. The petals on the upper layer can be heated and distilled by steam distilled out from the fresh petals at the bottom in the upward moving process, and working efficiency can be further improved.

The invention discloses plant stem cells for angelica plant storage root cambia as well as a preparation method and a culture method thereof. The preparation method comprises the following steps: slicing sterilized storage roots, placing sliced storage roots on a screening culture medium, processing so that the non-cambial cells in the root slices partially die or are damaged or stop growing; placing the slices into a separation culture medium for culturing so that the division of cambial cells is promoted and no dedifferentiation is caused, and preliminarily separating to obtain the cambial cells; transferring the cambial cells into a subculture medium for carrying out subculture, transferring the subcultured cambial cells into the separation culture medium for alternately and repeatedly culturing until a stable cell containing a large amount of small vacuoles is obtained. The method for culturing the cambial stem cells comprises a suspension culture method and a fermenter culture method. By adopting the preparation method, the separation of the angelica cambial stem cells is achieved, a new method is provided for the expanding culture of the angelica cambial stem cells and the preparation method lays the foundation for a large amount of industrial production of angelica plant storage root cambia stem cells and also provides a new source of cells for angelica plant storage root cambia stem cells.

The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. Accordingly, the cell line derived from the cambium of an herbaceous plant having a storage root makes it possible to produce large amounts of useful plants which are difficult to cultivate outdoor due to various problems associated with the period of cultivation, the selection of cultivation land, cultivation cost and the like.

The invention relates to the technical field of skin care products, in particular to quick-acting wrinkle repairing emulsion and a preparation method thereof. The quick-acting wrinkle repairing emulsion comprises azone, plant stem cell active matters, emulsifying agents, stabilizing agents, softening agents, wetting agents, preservatives, carbomer, arginine, octylene glycol and water. The emulsion has the advantages that injured cell tissues can be effectively repaired and supplemented, wrinkles are reduced, human aging is relieved, and effects are fine.

The invention discloses a method of promoting generation of lateral buds of a Heveabrasiliensis by a trans-AtWUS (Arabidopsisthaliana WUSCHEL) gene. Genetic transformation is carried out on a fragile embryonic callus of the Heveabrasiliensis by adopting the AtWUS gene to obtain a transgenetic material to promote generation of the lateral buds of the Heveabrasiliensis and to form lateral branches, so that the function of the AtWUS gene is verified, which shows that the WUS gene has an important effect on generation of the lateral buds and formation of the lateral branches of the Heveabrasiliensis. The lateral buds and lateral branches obtained have actual application potential. By means of the agrobacterium-mediated method, a plant expression vector is established to transfer the AtWUS gene to the callus of the Heveabrasiliensis to obtain the transgenetic material so as to promote somatic embryogenesis or organogenesis of the Heveabrasiliensis and promote the ability of forming a regeneration plant or a budling according to the property that the plant stem cell determinant gene WUS has a key effect on the meristem of a plant and promotes somatic embryogenesis and / or organogenesis. The method serves application and production from a novel path of genetic engineering of the Heveabrasiliensis.

The present invention relates to a cell line derived from the quiescent center of a plant and a method for isolating the same, more specifically, relates to a quiescent center-derived homogeneous cell line of single-cell origin, which is obtained from the quiescent center of a plant without needing a separate de-differentiation process, and a method for isolating the same.

The invention discloses a culture method of plant stem cells. The culture method of plant stem cells specifically comprises the following steps: S1, raw material selection; S2, seed cultivation; S3, seed treatment; S4, inductive separation; and S5, cultural proliferation. According to the culture method of plant stem cells disclosed by the invention, the method comprises the following steps: by taking fresh corns as a raw material, culturing the raw material to obtain germinated seeds; then selecting root tissues with static centers; and inducing to obtain static central stem cells. Compared with a conventional plant stem cell culture method, the culture method plant stem cells disclosed by the invention is simple and feasible, high in extraction efficiency and short in cultural period.

The invention provides isolated culture and extraction of plant stem cells of tomato and a preparation method of oral liquids, beverages and white spirit. As stem cells from a plant cambium of tomato is in an undifferentiated state, during amplified culture cells are high in hereditary stability, strong in activity, great in harvest rate and the like. The oral liquids, the beverages and the plant stem cell wine prepared by ethanol ultrasonic extraction have better use to resist cellular oxidation, prevent differentiated cells from turning to tumor cells, and reduce blood fat, blood pressure, cholesterol and e blood sugar.

The invention provides two flavonoid compounds, namely baicalein and scutellarein, of promoting a asymmetric cell division (ACD) regulation pathway of a root tip stem cell of a plant. The two compounds have effects of promoting SCR-SHR (scarecrow-short root) combination of an ACD active regulation complex of a root tip stem cell, does not influence RBR-SHR (retinoblastoma-related-short root) combination of a non-active complex and can activate NUC (nutcraker) expression of an ACD downstream key gene. At the same time, the invention provides an application of baicalein and scutellarein in preparing an SHR-SCR protein binding promoter and an NUC gene expression promoter. A secondary metabolism product of promoting ACD regulation of the root tip stem cell of the plant is found for the first time; a new biological effect of baicalein and scutellarein is found; an important tool is provided for an in-depth study of the stem cell regulation of the plant; and a new clue is provided for the development and application of a plant stem cell technology in industries of modern agriculture, biological medicine, hairdressing, health and the like.

The present invention relates to a composition for preventing or treating acquired immunodeficiency syndrome (AIDS), comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof, which are derived from a natural product, minimize adverse side effects of prior therapeutic agents and safe for the human body. Further, they effectively increase the count of T cells such as CD4+ T cells and decrease the number of HIVs, thereby preventing opportunistic infection, dysneuria, and neoplasia caused by immune incompetence, and ultimately the risk of death. Therefore, they are useful in preventing and treating AIDS, and relieving symptoms of AIDS.

The invention discloses a preparation method of a composition with anti-glycation and anti-skin aging functions. Characterized in that it is characterized in that it comprises, the feed additive is prepared from the following raw materials in parts by weight. 4-8 parts of astragalus membranaceus, 4-6 parts of radix ophiopogonis, 2-4 parts of radix angelicae, 6-10 parts of ligusticum wallichii, 4-8parts of liquorice, 2-4 parts of radix puerariae, 2-4 parts of dendrobe, 4-6 parts of polygonum multiflorum, 2-4 parts of poria cocos, 6-8 parts of atractylodes macrocephala, 8-10 parts of saffron crocus, 6-8 parts of rhodiola. The composition is simple to prepare, can effectively utilize epigenetic factors of plant stem cells to maintain skin efficacy, effectively enables epidermis to be in a young state, and eliminates glycoprotein.

he invention discloses antiaging cosmetics containing oligopeptide, capable of enabling skin to be compact. The antiaging cosmetics comprise freeze-dried powder and solvent liquid, wherein the freeze-dried powder consists of the following raw materials in percentage by mass of 0.3-5% of composite oligopeptide, 1.5-3% of a freeze-drying protective agent, 0.1-0.8% of disodium hydrogen phosphate, 0.2-1% of sodium dihydrogen phosphate and the balance water; and the solvent liquid consists of the following raw materials in percentage by mass of 0.8-8% of a natural plant composition, 0.2-0.5% of octanohydroxamic acid, 2-6% of glycerol caprylate, 1-3% of hydroxyacetophenone, 0.1-0.5% of sodium hyaluronate, 1-5% of butanediol and the balance water. According to the antiaging cosmetics containing oligopeptide, capable of enabling skin to be compact disclosed by the invention, oligopeptide-1, oligopeptide-3 and plant stem cells are compounded to a freeze-dried powder system, so that clearance rate for free radicals DPPH is high, and is as high as 98.1%, the skin aging can be effectively delayed, collagen protein synthesis can be promoted, the moisturizing function of skin can be improved, the skin moisture can be effectively maintained, the skin and muscle MMV value can be increased, the moisturizing system of the skin can be strengthened, the skin and muscle elasticity can be improved,the skin and muscle are compact and the skin and muscle fine lines can be smoothed.

Embodiments of the invention include plant stem cell products and treatment methods using the products. In some embodiments, the products may include extracts of plant stem cells, such as apple stem cells, and an aerosolizing device configured to create an aerosol from the plant stem cell products and to deliver the aerosol to the lung via inhalation. Treatment methods include aerosol inhalation of the plant stem cell products.