38 results about "Plant stem cell" patented technology

The present invention belongs to the field of daily chemicals, and discloses a mitochondrial energy improvement composition, application and cosmetic based on plant stem cells. The composition comprises the following components in parts by weight: 0.0005 - 1 part of sunflower bud extract; 0.0005 - 1 part of lemon balm extract; 0.0005 - 1 part of epigallocatechin gallate or epigallocatechin gallate. The composition of the present invention combines sunflower bud extract, lemon balm extract, epigallocatechin gallate or epigallocatechin gallate, and it is found that the combination of the three has obvious ability to improve cell mitochondrial dysfunction, enhance mitochondrial energy and increase the content of type I collagen, and can be used as a potential alternative for anti-wrinkle active ingredients in cosmetics.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

The invention relates to the technical field of biology, in particular to a method for cloning medicinal plant stem cells and obtaining a high-yield cell line, and the cell line with high yield of specific active substances is obtained through establishment of a suspension cell line, obtaining of single cells, cell cloning culture and screening of cloned cells.

The invention discloses a plant combination extract with whitening and anti-aging effects and a preparation method and application thereof, and particularly relates to the field of application of natural active substances, and the plant combination extract comprises a sterile tissue culture method of plant stem cells, a functional component enrichment strategy under regulation and control of an inducer, and a cell wall enzymolysis and grinding centrifugation synergistic extraction process. The invention also provides a synergistic application path of the obtained composite extract in multiple skin care mechanisms such as whitening, oxidation resistance, inflammation resistance, collagen metabolism regulation and the like. A compound active extract system is constructed by combining a multi-source induction enrichment mechanism of ginseng stem cells, centella stem cells and schizonepeta stem cells based on tissue culture with a plant cell wall enzymolysis extraction process, so that a natural, safe and efficient multi-channel synergistic whitening and anti-aging function is realized; therefore, the problems of single source of functional components, strong irritation, poor safety, insufficient long-term efficacy and the like in the existing product are effectively solved.

The invention discloses a composition containing stem cells and collagen and application of the composition in anti-aging products, and relates to the technical field of cosmetics, and the composition is prepared from 0.1-5 parts of umbilical cord mesenchymal stem cells, 1-20 parts of type I collagen, 3-15 parts of chitosan-hyaluronic acid microspheres, 1-10 parts of plant stem cell extract and 1-5 parts of exosome wrapped by liposome. Through component bionics, delivery system innovation and multi-target cooperation, the composition gives consideration to safety, effectiveness and use experience, and is expected to form differentiated competitiveness in a high-end anti-aging cosmetic market.

The invention discloses a plant stem cell nutrition synergist, and belongs to the technical field of plant stem cell nutrient solutions. The nutrient synergist can significantly improve the proliferation rate and survival rate of stem cells, promotes differentiation of the stem cells to target cell types, plays an important role in stem cell culture, can significantly improve the culture efficiency of the stem cells, provides comprehensive nutrient support and a suitable growth environment for stem cell culture, and has good application prospects. Proliferation and differentiation of the stem cells are promoted, and further research on the plant stem cells is facilitated.

The invention relates to an anti-aging skin-care matrix capable of repairing cell activity and a preparation method of the anti-aging skin-care matrix. The composition is specifically prepared from the following raw materials in parts by mass: 10 to 30 percent of nicotinamide adenine dinucleotide NAD < + > precursor, 5 to 15 percent of phospholipid modified fullerene, 1 to 5 percent of oligopeptide-3 solution, 3 to 8 percent of vitamin C magnesium phosphate, 2 to 6 percent of vitamin E acetate, 2 to 6 percent of plant stem cell extract prepared from apple tooth powder, 0.5 to 2 percent of sodium hyaluronate, 5 to 10 percent of glycerinum and the balance of water. 1-5% of microcrystalline cellulose, 0.5-2% of sodium carboxymethyl starch, 0.1-1% of magnesium stearate, 10-19% of vegetable fat, 0.1-1% of perfume and the balance of deionized water. Through the synergistic effect of all the components, the effects of accelerating metabolism, helping to homogenize the skin color, reducing darkness, resisting aging and wrinkles, fading fine wrinkles and improving skin elasticity are achieved.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging cell metabolism accelerating cosmetic and a preparation method thereof, and the technical scheme is that the anti-aging cell metabolism accelerating cosmetic comprises the following components: 0.0001%-0.005% of a cell growth factor compound, 0.0001%-0.003% of a small molecule peptide compound, 0.1%-5% of a plant stem cell extract, 1%-10% of grease, and the balance of water. 1%-10% of a surfactant, 1%-15% of a humectant, 0.1%-1% of an antioxidant, 0.1%-3% of a pH regulator, 0.05%-0.2% of a preservative and the balance of deionized water; epidermal and dermal layer cell proliferation is promoted through the cell growth factor, cell metabolism is regulated and controlled and collagen synthesis is promoted through the small molecule peptide, the plant stem cell extract is rich in polyphenol active ingredients and can activate the activity of skin endogenous stem cells, and the three components have a synergistic effect through different action pathways; the metabolism rate of skin cells and the anti-aging effect are obviously improved.

The invention discloses a whitening and anti-aging mask formula based on dogwood. The whitening and anti-aging mask formula based on dogwood is prepared from the following raw materials in parts by mass: 5 to 15 parts of dogwood extract, 2 to 8 parts of vitamin C powder, 1 to 3 parts of polyphenol compound, 1 to 5 parts of glycerinum, 2 to 4 parts of hyaluronic acid, 3 to 9 parts of plant essence, 2 to 6 parts of vegetable oil, 4 to 12 parts of seaweed extract and 1 to 3 parts of polyether-201. 1-3 parts of nerve growth factors, 2-6 parts of DNA (deoxyribonucleic acid) repair enzyme, 0.1-0.3 part of snake venom essence, 1-3 parts of plant stem cells and 20-30 parts of deionized water, components in the snake venom essence can stimulate synthesis of collagen and increase skin elasticity, so that wrinkles and fine wrinkles are reduced, the skin looks younger and tighter, certain antioxidant capacity is achieved, and the skin care effect is good. Free radicals can be neutralized, the damage of environmental pollution and ultraviolet radiation to the skin is reduced, and the aging process of the skin is delayed.

The utility model belongs to the field of plant cell culture, and particularly discloses a bioreactor for efficient suspension culture of plant stem cells, which comprises a main body, a base, a culture container arranged at the top of the base, an exhaust port arranged at the top of the culture container, and an air inlet arranged at the bottom of the culture container, the air stone is arranged in the air inlet; the sampling mechanism comprises a sampling pipe arranged on one side of the air inlet, a fixing ring arranged on the periphery of the sampling pipe, a sleeve rotationally arranged on the fixing ring, a discharging opening formed in the bottom of the sampling pipe and a discharging opening formed in the sleeve; according to the utility model, by rotating the sleeve, the discharge port is aligned with the discharge port, so that a culture solution can flow into an external container to complete sampling work, and by arranging the three discharge ports with different sizes, overflow waste is avoided while the sampling speed is ensured, and personnel can conveniently and accurately control the sampling amount.

The application discloses a kind of Pterocarpus stem cell culture methods.The culture method includes the following steps: (1) stem cell maintenance factor composition and temperature-sensitive liposome are added to hydrogel precursor solution to be frozen into shape and prepare porous three-dimensional scaffold precursor;After the porous three-dimensional scaffold precursor is crosslinked and solidified, three-dimensional biomimetic niche scaffold is prepared;(2) the explant of Pterocarpus is inoculated in three-dimensional biomimetic niche scaffold and is induced to culture and obtains the stem cell of Pterocarpus plant induced to form;(3) the stem cell of Pterocarpus plant induced to form is taken out, then undifferentiated Pterocarpus plant stem cell mass is filtered and screened by flow cytometry;(4) Pterocarpus plant stem cell mass is placed in liquid proliferation medium and is suspended in culture on shaker, and obtains the stem cell of Pterocarpus plant after proliferation;(5) the stem cell of Pterocarpus plant after proliferation is transferred to production culture medium and is dark culture, and secondary metabolite accumulation is induced.

The invention particularly relates to a deep moisturizing skin moisturizer based on compound plant essence and a preparation method of the deep moisturizing skin moisturizer. The skin moisturizer is prepared from water, dipropylene glycol, glycerol, betaine, panthenol, trehalose, acetylchitosamine, tetrahydromethylpyrimidine carboxylic acid (Ectoin), crithmum maritimum stem cell extract, aloe extract, witch hazel extract, dipotassium glycyrrhizinate, nicotinamide, a thickening agent, a preservative and a pH regulator. According to the skin moisturizer disclosed by the invention, through innovative component compounding and component synergism, the integrated effect of'moisturizing, water locking, repairing and protecting 'is realized, instant moisturizing is realized, long-acting water locking, skin barrier repairing, anti-inflammatory and anti-photoaging effects can be integrated, and the skin state can be improved for a long time. Components obtained by adopting rare plant stem cells and a plant extraction technology are green and sustainable, and the efficacy density is far higher than that of a traditional product with a single function; the traditional Chinese medicine composition is remarkable in effect, mild and safe and has great market competitiveness.

A method for manufacturing a dressing that promotes wound healing comprises at least the following steps: providing a container; adding purified water to the container; adding a first active ingredient to the container, wherein the first active ingredient is low-molecular-weight hyaluronic acid having a molecular weight of not more than 500,000 Daltons, and which accounts for 0.01% to 1% by weight of the entire dressing; and adding a second active ingredient to the container, wherein the second active ingredient is β-nicotinamide mononucleotide and accounts for 0.01% to 1% by weight of the entire dressing; or the second active ingredient is a plant stem cell extract and accounts for 0.1% to 5% by weight of the entire dressing.

The invention discloses a culture medium and a culture method of orchid stem cells. The culture medium comprises a basic culture medium, and 100-300 mg / L of lanthanum nitrate is added into the basic culture medium; the basic culture medium is selected from one of an MS culture medium and a B5 culture medium. The basic culture medium is an MS culture medium, 0.9-1.2 mg / L of amphotericin B and a double antibody with the volume concentration of 0.8%-1.3% are further added, and the double antibody is a 100X mycillin mixed solution, 0.9-1.1 mg / L of 6-BA and 0.9-1.2 mg / L of NAA; or the basic culture medium is a B5 culture medium, and 0.099 mg / L to 0.130 mg / L of 6-BA, 12 mg / L to 17 mg / L of cane sugar, 0.49 mg / L to 0.54 mg / L of methyl jasmonate and 0.098 mg / L to 0.120 mg / L of triacontanol are also added into the basic culture medium. Lanthanum nitrate with the concentration of 100-300 mg / L is added into the culture medium for culturing the orchid stem cells, and experiments show that a large number of stem cells can be cultured within a short time by adding lanthanum nitrate with the concentration range, the cell dispersion degree is increased, the number of the cells is increased, and a foundation is provided for subculture multiplication culture in the next step.

The invention discloses an ovary and gonad axis function repairing preparation and a preparation process thereof, and relates to the technical field of biology, the preparation comprises the following components by weight: 22-38 parts of a marine organism polysaccharide composite raw material; 12-28 parts of a plant stem cell extract; 4 to 10 parts of deep sea sponge terpenoids; 3-7 parts of coral polysaccharide; 0.008 to 0.018 part of vitamin C palmitate; 0.4 to 0.6 part of xanthan gum; the marine organism polysaccharide composite raw material consists of fucoidan and fucoidan in a weight ratio of (1: 1)-(3: 1). The plant stem cell extract consists of a ginseng stem cell extract and an angelica sinensis stem cell extract in a weight ratio of (3: 1)-(4: 1); through compatibility of the composite marine polysaccharide, the plant stem cell extract and other components, a repair-activation-protection closed loop is formed, and the limitation of a single component is broken through; by adopting processes such as low-temperature extraction and supercritical technology, the activity is retained to the maximum extent, the curative effect and safety of the preparation are improved, and an efficient treatment choice is provided for ovarian function decline.

The invention relates to cosmetic and dermocosmetic compositions comprising stem cell-derived callus extracts from Punica granatum obtained via standardized in vitro plant stem cell cultures. The extracts are rich in polyphenols, flavonoids, ellagic acid, anthocyanins, tannins, vitamins C, E, and K, and amino acids, providing potent antioxidant, anti-inflammatory, regenerative, moisturizing, and brightening effects. These compositions stimulate collagen synthesis, enhance skin elasticity and firmness, protect against oxidative stress, reduce redness and irritation, and promote overall skin renewal. Formulations include aqueous-based ampoules, essences, or serums containing 0.5-5% by weight of the extract, optionally combined with humectants, skin-soothing agents, antioxidants, and stabilizers. The invention further discloses optimized methods for callus induction, extraction, and formulation, ensuring reproducibility, high bioactive yield, stability, scalability, and sustainable production. This provides a scientifically validated, eco-friendly cosmetic solution delivering comprehensive skin benefits, addressing anti-aging, regeneration, hydration, and brightening needs.

This invention provides a method for culturing embryonic stem cells from Orobanchaceae plants. The steps include: obtaining explants from Orobanchaceae plants; inducing culture of the explants to obtain embryonic stem cells; and inoculating the embryonic stem cells into an expansion medium containing Artemisia annua extract for further culture. This invention provides, for the first time, a method for the isolation and rapid culture of embryonic stem cells from Orobanchaceae plants. This method is independent of host plants and can rapidly and massively obtain Orobanchaceae plant stem cells under artificial conditions, fundamentally solving the problem of ecological damage caused by traditional production methods. For the first time, Artemisia annua extract is applied to the culture of Orobanchaceae plant stem cells. Utilizing its natural antibacterial and bioactivity, it not only effectively inhibits microbial contamination during the culture process but also unexpectedly shows that it can significantly promote the growth of Orobanchaceae plant stem cells, shorten the culture cycle, and reduce production costs.

A novel nanocomposite spray formulation developed for advanced wound healing by combining the therapeutic potential of plant stem cell extract with innovative polymeric film-forming technology, the novel nanocomposite spray formulation comprising the following: at least one plant stem cell extract, selected from Centella asiatica stem cell extract and Curcuma longa stem cell extract; and a polymeric film-forming matrix comprising ethylcellulose and Eudragit, wherein the formulation, when applied to a wound surface, forms a thin, transparent and adhesive film, thereby promoting accelerated wound healing.

The invention discloses a freezing positioning culture method of azaleaceae plant stem cells and application of the freezing positioning culture method, and belongs to the technical field of plant stem cells. In order to solve the problems of inaccurate stem cell positioning, low induction efficiency and pollution in the prior art, paraffin embedding is combined with a liquid nitrogen freezing slicing technology, the multi-vacuole stem cells in young fruits are accurately positioned through neutral red staining, and the extraction success rate exceeds 85%. Inducing medium components (containing 80-120 mg / L of ascorbic acid, 1-3 mg / L of IAA and 2-6 mg / L of glutamic acid / arginine / cysteine combination) are optimized, so that the callus formation time is shortened to 2-7 days, and the efficiency is improved by 4 times compared with that of a traditional method. In the amplification stage, amphotericin B (2-6 mg / L) is added, liquid is supplemented in stages for culture, and the pollution rate is lower than 5%. The obtained stem cells are directly used for eye protection chewable tablets (containing 20-30% of stem cells) after being dried, and clinical tests show that the asthenopia score can be reduced by 19.3% (Plt; 0.01) after the eye protection chewable tablets are continuously taken for 2 weeks. The method is suitable for blueberries, rhododendron, bilberries and the like, breaks through the limitation of fresh fruit processing, and provides a new way for functional food development.

Provided is a method for manufacturing a dressing for promoting wound healing, comprising at least the following steps: providing a container; adding purified water to the container; adding a first active ingredient to the container, wherein the first active ingredient is low-molecular-weight hyaluronic acid (HA), which has a molecular weight of 500,000 daltons or less and accounts for 0.01 to 1 weight percentage of the entire dressing; and adding a second active ingredient to the container, wherein the second active ingredient is β-nicotinamide mononucleotide (β-NMN), which accounts for 0.01 to 1 weight percentage of the entire dressing, or a plant stem cell extract, which accounts for 0.1 to 5 weight percentage of the entire dressing. (FIG. 1)

The present application relates to the technical field of biology, in particular to a method for obtaining a medicinal plant stem cell clone and a high-yield cell line, which adopts the establishment of a suspension cell line, the acquisition of single cells, cell clone culture and the screening of clone cells to obtain a cell line with high yield of specific active substances.

The utility model discloses a plant stem cell exosome extraction liquid supplementing mechanism, which relates to the technical field of plant stem cell exosome extraction, and is characterized in that a motor rotates by a certain angle to drive a rotating roller to rotate, so that a connecting rod slides in a wave-shaped sliding chute, and a sliding rod is driven to drive a piston to lift; the highest points of the wave-shaped sliding groove are located on the same horizontal line, and the lowest points of the wave-shaped sliding groove are located on the same horizontal line, so that the motor rotates by the same angle, the lifting distance of the piston is the same, accurate control over the supplementing amount can be achieved, automatic operation is achieved, manual operation is not needed, and the working efficiency is improved. The liquid supplementing time is shortened, so that the working efficiency of extracting and supplementing the liquid for the exosome of the plant stem cells is improved.

The invention belongs to the technical field of plant stem cell culture, and particularly relates to an isolation and differentiation culture method of salvia miltiorrhiza root cambium stem cells, which comprises the following steps: cutting the root of a wild salvia miltiorrhiza plant into sections, washing and sterilizing, cutting into small sections, sterilizing for the second time, and washing to obtain a sterile explant. Or carrying out pretreatment, surface sterilization and culture on salvia miltiorrhiza seeds to obtain sterile seedlings, carrying out expanding propagation and rooting and seedling strengthening culture to obtain sterile plants, and cutting the roots of the sterile plants into sections to obtain the sterile explants. And soaking the sterile explant in a preserving fluid, and then carrying out induction culture, subculture and suspension culture to obtain the salvia miltiorrhiza root cambium stem cells. Wherein the preserving fluid, the induction culture medium, the subculture medium and the suspension culture medium all contain plant hormones. And the induction culture medium also contains REF1. According to the separation and differentiation culture method of the salvia miltiorrhiza root cambium stem cells, cell browning can be effectively prevented, and the cultured tissue cells are stable in gene expression and growth.

The invention discloses an acidic hydrogen peroxide alcohol hand disinfectant and a preparation method thereof, and relates to the technical field of disinfectants. The cleaning agent is prepared from the following components in percentage by mass: 0.1-3% of hydrogen peroxide, 0.05-1% of an acidity enhancer, 50-80% of an alcohol substance, 0.1-3% of glycerol, 0.1-5% of a film-forming agent, 0.1-2% of a repairing component, 0.02-1% of a stabilizer and the balance of deionized water, wherein the acidity enhancer is citric acid, lactic acid or malic acid, the film-forming agent is tara gum, tamarind gum or a sodium alginate derivative, and the repairing component is a plant stem cell extract, an acid algae extract or a sargassum muticum extract. According to the invention, three problems that a low-concentration hydrogen peroxide hand disinfectant, an alcohol hand disinfectant and a hydrogen peroxide quick-drying hand disinfectant cannot achieve an inactivation effect on non-enveloped viruses in the presence of organic pollutants, skin irritation is easily caused, and hydrogen peroxide is easily decomposed and fails in a system are solved.

The invention provides application of silicon dioxide nanoparticles in promoting moss leaf incision stem cell development and a method, and relates to the technical field of plant regeneration. Depending on the totipotency of the plant stem cells, the silicon dioxide nanoparticles are used for promoting the development of the stem cells of the in-vitro moss leaves subjected to mechanical damage, promoting the rapid growth of leaf incisions, generating more new growth points, remarkably increasing the number of the epiphytic points of the newly born protonema of the leaves, shortening the cultivation time and improving the cultivation efficiency; and an effective nanometer strategy is provided for rapid propagation of moss.

The invention discloses a biological enzyme decomposition agent and a culture method. The biological enzyme decomposition agent is prepared from the following components in parts by weight: 20 to 30 parts of plant stem cell extract, 30 to 40 parts of nattokinase, 10 to 15 parts of papain, 5 to 10 parts of cellulase, 5 to 10 parts of lipase, 5 to 1 part of penetrant and 5 to 10 parts of plant extract essence. The compound enzyme preparation is composed of a plurality of components in a synergistic manner, has strong degradation capability, can efficiently treat a plurality of complex mixed organic wastes, and overcomes the function limitation of a single enzyme preparation; the plant extract essence endows the composition with good environmental adaptability, can keep high activity under different extreme conditions, and is wide in application scene; a matched efficient culture process is adopted, links from raw material pretreatment to extraction and purification are strictly controlled, high quality and stability of the product are ensured, and the yield and the purity are improved; by means of the characteristics, the method has wide application prospects in the fields of environmental protection, industry and the like, and the whole degradation process is green and pollution-free.

Disclosed herein is a method of isolating a stem cell line derived from a tissue from a primary meristem in a leaf of a plant, including the steps of sterilizing at least a part of the leaf containing the central or other vein; cutting through the central or other vein of the leaf to expose primary meristematic cells from the central or other vein inner vascular tissue; and culturing cells from said exposed cells, which circumvents the difficulties of surface sterilization when other types of explants are used. Also disclosed herein are stem cell lines obtained using the method.

The invention belongs to the field of plant stem cell regeneration, and discloses a method for inducing tetrasynthum mongolicum stem cells to form regenerated plants.The method comprises the steps that tetrasynthum mongolicum stem cells are obtained after tetrasynthum mongolicum explants are cultured through an induction culture medium and a subculture medium, and after the tetrasynthum mongolicum stem cells are subjected to suspension culture, callus tissue is obtained through induction of a callus culture medium; inoculating the calluses into a culture medium containing a regeneration factor REF1, differentiating into embryogenic calluses of the tetrasyna mongolica, and transferring the embryogenic calluses of the tetrasyna mongolica into an embryoid induction culture medium to obtain embryoids of the tetrasyna mongolica; inoculating the embryoid into a bud proliferation culture medium to obtain clumpy buds; and inoculating the clumpy buds into an elongation rooting culture medium for subculture to obtain the tetracalamus mongolicus seedlings. Compared with a traditional method for performing tissue culture by using sterile seedlings, the method disclosed by the invention has the advantages that stem cells are utilized for performing regeneration on the tetrasyna mongolica plant for the first time, excellent cell division and differentiation capabilities are shown, the characters of a female parent can be kept, and long-term growth can be maintained.