21 results about "Plant stem cell" patented technology

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

The invention discloses a plant stem cell nutrition synergist, and belongs to the technical field of plant stem cell nutrient solutions. The nutrient synergist can significantly improve the proliferation rate and survival rate of stem cells, promotes differentiation of the stem cells to target cell types, plays an important role in stem cell culture, can significantly improve the culture efficiency of the stem cells, provides comprehensive nutrient support and a suitable growth environment for stem cell culture, and has good application prospects. Proliferation and differentiation of the stem cells are promoted, and further research on the plant stem cells is facilitated.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging cell metabolism accelerating cosmetic and a preparation method thereof, and the technical scheme is that the anti-aging cell metabolism accelerating cosmetic comprises the following components: 0.0001%-0.005% of a cell growth factor compound, 0.0001%-0.003% of a small molecule peptide compound, 0.1%-5% of a plant stem cell extract, 1%-10% of grease, and the balance of water. 1%-10% of a surfactant, 1%-15% of a humectant, 0.1%-1% of an antioxidant, 0.1%-3% of a pH regulator, 0.05%-0.2% of a preservative and the balance of deionized water; epidermal and dermal layer cell proliferation is promoted through the cell growth factor, cell metabolism is regulated and controlled and collagen synthesis is promoted through the small molecule peptide, the plant stem cell extract is rich in polyphenol active ingredients and can activate the activity of skin endogenous stem cells, and the three components have a synergistic effect through different action pathways; the metabolism rate of skin cells and the anti-aging effect are obviously improved.

The invention discloses a whitening and anti-aging mask formula based on dogwood. The whitening and anti-aging mask formula based on dogwood is prepared from the following raw materials in parts by mass: 5 to 15 parts of dogwood extract, 2 to 8 parts of vitamin C powder, 1 to 3 parts of polyphenol compound, 1 to 5 parts of glycerinum, 2 to 4 parts of hyaluronic acid, 3 to 9 parts of plant essence, 2 to 6 parts of vegetable oil, 4 to 12 parts of seaweed extract and 1 to 3 parts of polyether-201. 1-3 parts of nerve growth factors, 2-6 parts of DNA (deoxyribonucleic acid) repair enzyme, 0.1-0.3 part of snake venom essence, 1-3 parts of plant stem cells and 20-30 parts of deionized water, components in the snake venom essence can stimulate synthesis of collagen and increase skin elasticity, so that wrinkles and fine wrinkles are reduced, the skin looks younger and tighter, certain antioxidant capacity is achieved, and the skin care effect is good. Free radicals can be neutralized, the damage of environmental pollution and ultraviolet radiation to the skin is reduced, and the aging process of the skin is delayed.

The application discloses a kind of Pterocarpus stem cell culture methods.The culture method includes the following steps: (1) stem cell maintenance factor composition and temperature-sensitive liposome are added to hydrogel precursor solution to be frozen into shape and prepare porous three-dimensional scaffold precursor;After the porous three-dimensional scaffold precursor is crosslinked and solidified, three-dimensional biomimetic niche scaffold is prepared;(2) the explant of Pterocarpus is inoculated in three-dimensional biomimetic niche scaffold and is induced to culture and obtains the stem cell of Pterocarpus plant induced to form;(3) the stem cell of Pterocarpus plant induced to form is taken out, then undifferentiated Pterocarpus plant stem cell mass is filtered and screened by flow cytometry;(4) Pterocarpus plant stem cell mass is placed in liquid proliferation medium and is suspended in culture on shaker, and obtains the stem cell of Pterocarpus plant after proliferation;(5) the stem cell of Pterocarpus plant after proliferation is transferred to production culture medium and is dark culture, and secondary metabolite accumulation is induced.

The invention particularly relates to a deep moisturizing skin moisturizer based on compound plant essence and a preparation method of the deep moisturizing skin moisturizer. The skin moisturizer is prepared from water, dipropylene glycol, glycerol, betaine, panthenol, trehalose, acetylchitosamine, tetrahydromethylpyrimidine carboxylic acid (Ectoin), crithmum maritimum stem cell extract, aloe extract, witch hazel extract, dipotassium glycyrrhizinate, nicotinamide, a thickening agent, a preservative and a pH regulator. According to the skin moisturizer disclosed by the invention, through innovative component compounding and component synergism, the integrated effect of'moisturizing, water locking, repairing and protecting 'is realized, instant moisturizing is realized, long-acting water locking, skin barrier repairing, anti-inflammatory and anti-photoaging effects can be integrated, and the skin state can be improved for a long time. Components obtained by adopting rare plant stem cells and a plant extraction technology are green and sustainable, and the efficacy density is far higher than that of a traditional product with a single function; the traditional Chinese medicine composition is remarkable in effect, mild and safe and has great market competitiveness.

A method for manufacturing a dressing that promotes wound healing comprises at least the following steps: providing a container; adding purified water to the container; adding a first active ingredient to the container, wherein the first active ingredient is low-molecular-weight hyaluronic acid having a molecular weight of not more than 500,000 Daltons, and which accounts for 0.01% to 1% by weight of the entire dressing; and adding a second active ingredient to the container, wherein the second active ingredient is β-nicotinamide mononucleotide and accounts for 0.01% to 1% by weight of the entire dressing; or the second active ingredient is a plant stem cell extract and accounts for 0.1% to 5% by weight of the entire dressing.

The invention discloses an ovary and gonad axis function repairing preparation and a preparation process thereof, and relates to the technical field of biology, the preparation comprises the following components by weight: 22-38 parts of a marine organism polysaccharide composite raw material; 12-28 parts of a plant stem cell extract; 4 to 10 parts of deep sea sponge terpenoids; 3-7 parts of coral polysaccharide; 0.008 to 0.018 part of vitamin C palmitate; 0.4 to 0.6 part of xanthan gum; the marine organism polysaccharide composite raw material consists of fucoidan and fucoidan in a weight ratio of (1: 1)-(3: 1). The plant stem cell extract consists of a ginseng stem cell extract and an angelica sinensis stem cell extract in a weight ratio of (3: 1)-(4: 1); through compatibility of the composite marine polysaccharide, the plant stem cell extract and other components, a repair-activation-protection closed loop is formed, and the limitation of a single component is broken through; by adopting processes such as low-temperature extraction and supercritical technology, the activity is retained to the maximum extent, the curative effect and safety of the preparation are improved, and an efficient treatment choice is provided for ovarian function decline.

The invention relates to cosmetic and dermocosmetic compositions comprising stem cell-derived callus extracts from Punica granatum obtained via standardized in vitro plant stem cell cultures. The extracts are rich in polyphenols, flavonoids, ellagic acid, anthocyanins, tannins, vitamins C, E, and K, and amino acids, providing potent antioxidant, anti-inflammatory, regenerative, moisturizing, and brightening effects. These compositions stimulate collagen synthesis, enhance skin elasticity and firmness, protect against oxidative stress, reduce redness and irritation, and promote overall skin renewal. Formulations include aqueous-based ampoules, essences, or serums containing 0.5-5% by weight of the extract, optionally combined with humectants, skin-soothing agents, antioxidants, and stabilizers. The invention further discloses optimized methods for callus induction, extraction, and formulation, ensuring reproducibility, high bioactive yield, stability, scalability, and sustainable production. This provides a scientifically validated, eco-friendly cosmetic solution delivering comprehensive skin benefits, addressing anti-aging, regeneration, hydration, and brightening needs.

This invention provides a method for culturing embryonic stem cells from Orobanchaceae plants. The steps include: obtaining explants from Orobanchaceae plants; inducing culture of the explants to obtain embryonic stem cells; and inoculating the embryonic stem cells into an expansion medium containing Artemisia annua extract for further culture. This invention provides, for the first time, a method for the isolation and rapid culture of embryonic stem cells from Orobanchaceae plants. This method is independent of host plants and can rapidly and massively obtain Orobanchaceae plant stem cells under artificial conditions, fundamentally solving the problem of ecological damage caused by traditional production methods. For the first time, Artemisia annua extract is applied to the culture of Orobanchaceae plant stem cells. Utilizing its natural antibacterial and bioactivity, it not only effectively inhibits microbial contamination during the culture process but also unexpectedly shows that it can significantly promote the growth of Orobanchaceae plant stem cells, shorten the culture cycle, and reduce production costs.

A novel nanocomposite spray formulation developed for advanced wound healing by combining the therapeutic potential of plant stem cell extract with innovative polymeric film-forming technology, the novel nanocomposite spray formulation comprising the following: at least one plant stem cell extract, selected from Centella asiatica stem cell extract and Curcuma longa stem cell extract; and a polymeric film-forming matrix comprising ethylcellulose and Eudragit, wherein the formulation, when applied to a wound surface, forms a thin, transparent and adhesive film, thereby promoting accelerated wound healing.

Provided is a method for manufacturing a dressing for promoting wound healing, comprising at least the following steps: providing a container; adding purified water to the container; adding a first active ingredient to the container, wherein the first active ingredient is low-molecular-weight hyaluronic acid (HA), which has a molecular weight of 500,000 daltons or less and accounts for 0.01 to 1 weight percentage of the entire dressing; and adding a second active ingredient to the container, wherein the second active ingredient is β-nicotinamide mononucleotide (β-NMN), which accounts for 0.01 to 1 weight percentage of the entire dressing, or a plant stem cell extract, which accounts for 0.1 to 5 weight percentage of the entire dressing. (FIG. 1)

The present application relates to the technical field of biology, in particular to a method for obtaining a medicinal plant stem cell clone and a high-yield cell line, which adopts the establishment of a suspension cell line, the acquisition of single cells, cell clone culture and the screening of clone cells to obtain a cell line with high yield of specific active substances.

The invention belongs to the technical field of plant stem cell culture, and particularly relates to an isolation and differentiation culture method of salvia miltiorrhiza root cambium stem cells, which comprises the following steps: cutting the root of a wild salvia miltiorrhiza plant into sections, washing and sterilizing, cutting into small sections, sterilizing for the second time, and washing to obtain a sterile explant. Or carrying out pretreatment, surface sterilization and culture on salvia miltiorrhiza seeds to obtain sterile seedlings, carrying out expanding propagation and rooting and seedling strengthening culture to obtain sterile plants, and cutting the roots of the sterile plants into sections to obtain the sterile explants. And soaking the sterile explant in a preserving fluid, and then carrying out induction culture, subculture and suspension culture to obtain the salvia miltiorrhiza root cambium stem cells. Wherein the preserving fluid, the induction culture medium, the subculture medium and the suspension culture medium all contain plant hormones. And the induction culture medium also contains REF1. According to the separation and differentiation culture method of the salvia miltiorrhiza root cambium stem cells, cell browning can be effectively prevented, and the cultured tissue cells are stable in gene expression and growth.

The invention provides application of silicon dioxide nanoparticles in promoting moss leaf incision stem cell development and a method, and relates to the technical field of plant regeneration. Depending on the totipotency of the plant stem cells, the silicon dioxide nanoparticles are used for promoting the development of the stem cells of the in-vitro moss leaves subjected to mechanical damage, promoting the rapid growth of leaf incisions, generating more new growth points, remarkably increasing the number of the epiphytic points of the newly born protonema of the leaves, shortening the cultivation time and improving the cultivation efficiency; and an effective nanometer strategy is provided for rapid propagation of moss.

The invention discloses a biological enzyme decomposition agent and a culture method. The biological enzyme decomposition agent is prepared from the following components in parts by weight: 20 to 30 parts of plant stem cell extract, 30 to 40 parts of nattokinase, 10 to 15 parts of papain, 5 to 10 parts of cellulase, 5 to 10 parts of lipase, 5 to 1 part of penetrant and 5 to 10 parts of plant extract essence. The compound enzyme preparation is composed of a plurality of components in a synergistic manner, has strong degradation capability, can efficiently treat a plurality of complex mixed organic wastes, and overcomes the function limitation of a single enzyme preparation; the plant extract essence endows the composition with good environmental adaptability, can keep high activity under different extreme conditions, and is wide in application scene; a matched efficient culture process is adopted, links from raw material pretreatment to extraction and purification are strictly controlled, high quality and stability of the product are ensured, and the yield and the purity are improved; by means of the characteristics, the method has wide application prospects in the fields of environmental protection, industry and the like, and the whole degradation process is green and pollution-free.

Disclosed herein is a method of isolating a stem cell line derived from a tissue from a primary meristem in a leaf of a plant, including the steps of sterilizing at least a part of the leaf containing the central or other vein; cutting through the central or other vein of the leaf to expose primary meristematic cells from the central or other vein inner vascular tissue; and culturing cells from said exposed cells, which circumvents the difficulties of surface sterilization when other types of explants are used. Also disclosed herein are stem cell lines obtained using the method.

The invention belongs to a plant stem cell culture technology, and particularly discloses a tissue culture radix paeoniae alba root stem cell separation and culture method. The method comprises the following steps: carrying out low-temperature and blue light pretreatment on an explant; a WPM culture medium of a specific additive composition (containing seaweed oligosaccharide, methyl jasmonate, glutathione, salicylic acid, ascorbic acid, nitric oxide donor SNP and the like) is used for induction culture; and carrying out liquid proliferation, purification and multiplication culture to obtain the high-purity radix paeoniae alba root stem cells. According to the method, the technical bottlenecks of low separation efficiency, easy browning, high pollution rate, immature system and the like of the traditional method are effectively solved, an efficient, stable and repeatable industrial production scheme of the radix paeoniae alba stem cells is provided, and a new technical approach is provided for standardized preparation and development of medicinal components of radix paeoniae alba.

The invention relates to a hair composition for cutaneous and follicular penetration, characterized in that it comprises: (A) 45% to 95% by weight of exosomes derived from plant stem cells, (B) 2% to 40% by weight of a fruit extract from the Serenoa palm, (C) 1% to 15% by weight of a hair root culture extract from Ocimum Basilicum, (D) 0.05% to 10% by weight of Niacinamide, (E) 0.05% to 10% by weight of Rosemary essential oil, the weight percentages being expressed as a percentage of the sum of the weights of (A), (B), (C), (D), and (E), as well as its use in the prevention or reduction of hair loss, the cosmetic use of the composition to increase hair density or hair diameter, or a cosmetic hair treatment method comprising the application of the composition to the scalp scalp massage, possibly followed by a scalp massage.

The present invention relates to a cosmetic composition containing a plant stem cell culture extract and an osmocell encapsulating lactic acid bacteria exosomes. Specifically, the present invention relates to a cosmetic composition for preventing skin aging, which is prepared by forming a lipid complex paste from cell membrane lipid components and water using a high-pressure emulsifier, and then adding the lipid complex to the aqueous solution of plant stem cell culture extract and lactic acid bacteria exosomes at room temperature to phase-invert the reverse micelle emulsion and stabilize it.