Plant stem cell derived from cambium of family solanaceae, and method for isolating and culturing same

A cambium and stem cell technology, applied in plant cells, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as high production costs, limited content of physiologically active substances, and variation

Inactive Publication Date: 2012-05-16
UNHWA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the content of physiologically active substances in plants is extremely limited
Second, plants grow more slowly than usual
Fourth, it involves the environmental problem known as destruction of nature
Fifth, in the case of plant-derived physiologically active substances, the chemical structure is very complicated, requiring a multi-stage polymerization process and there is an economical problem that the production cost is very high
Callus (Callus) is a tissue formed by dedifferentiation, for which the registered patent still has the problem of variation caused by dedifferentiation

Method used

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  • Plant stem cell derived from cambium of family solanaceae, and method for isolating and culturing same
  • Plant stem cell derived from cambium of family solanaceae, and method for isolating and culturing same
  • Plant stem cell derived from cambium of family solanaceae, and method for isolating and culturing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] [Example 1: Preparation of cambium-derived stem cells of Solanaceae]

[0049] 【1-1: Preparation of plant materials】

[0050] To harvest cambium-derived stem cells from tomato (Lycopericum esculentum, purchased from Sejong Seedlings Co., Ltd.) of the genus Solanaceae, the stem ( figure 1 A) Immediately thereafter, it was immersed in an antioxidant 100 mg / L ascorbic acid (L-ascorbic acid, DUCHEFA, TheNetherlands) solution for transport-preservation. At this time, to harvest the same stem cells for utilizing the procambium, the residual branches were collected instead of the stems.

[0051] Thereafter, in 0.1% benomyl (benomyl, Dongbu Hannong Chemical, Korea), 0.1% chlorothalonil (daconil, Dongbu Hannong Chemical, Korea), 0.1% streptomycin sulphate (sterptomycin sulphate, DUCHEFA, The Netherlands), 0.01% After pretreatment in a mixed solution of cefotaxime sodium DUCHEFA, The Netherlands for 10 minutes, they were washed with tap water for 5 minutes to remove phenolic com...

Embodiment 2

[0062] [Example 2: Proliferation and characteristic observation of stem cells derived from cambium of Solanaceae]

[0063] The cambium-derived stem cells isolated in Example 1 were put into bottles containing the following liquid medium and cultured in a shaker at 100 rpm at 25±1° C. under dark conditions. The cycle of subculture is fixed at 2 weeks, so that the cultured cells can always maintain high viability in the logarithmic growth phase.

[0064] Table 2: Suspension medium (medium 2)

[0065]

[0066] When observing the degree of cell aggregation (biological microscope C × 31, Olympus, Japan), the stem cells according to the present invention are as follows: figure 2 As shown in A, it was confirmed that a large number of single cells were included in the suspension culture, and some of them existed as cell aggregates of very small size. That is, as a result of culturing the stem cells according to the present invention, the maximum aggregate size was only 500 μm. ...

Embodiment 3

[0079] [Example 3: Preparation of extracts from cambium-derived stem cells of Solanaceae]

[0080] After harvesting the stem cells cultured in suspension for 14 days as in Example 2 and removing the culture medium, 500 ml of DMSO was added to 500 g of harvested stem cells and stirred at 50° C. for 6 hours to dissolve them. Thereafter, the supernatant was collected by centrifugation at 3,000 g for 10 minutes to obtain a DMSO-soluble substance. The obtained DMSO-soluble matter was concentrated with a rotary vacuum concentrator, and the concentrated sample was dried with a freeze dryer to obtain a DMSO extract and use it.

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Abstract

The present invention relates to a plant stem cell derived from the cambium of family Solanaceae, and to a method for isolating and culturing the same. A stem cell derived from the cambium of family Solanaceae, according to the present invention, is useful as it can be isolated in an undifferentiated state without undergoing a dedifferentiation process and stably maintained without causing any variation in the cell growth rate and growth pattern even during long-term culture, thereby enabling large-scale culture. Moreover, the stem cell derived from the cambium of family Solanaceae and the culture thereof, according to the present invention, are useful for preventing and delaying aging as they have the effects of inducing biosynthesis of procollagen and more efficiently inhibiting the generation of enzymes related to aging than retinoic acid, which is known as having an excellent effect in skin wrinkle improvement.

Description

【Technical field】 [0001] The present invention relates to cambium-derived stem cells of the family Solanaceae and a method for their isolation and culture. 【Background technique】 [0002] Plants used to mean food resources, but now they are sources of a wide spectrum of chemical substances including medicines, fragrances, pigments, pesticides, pigments, etc., and their meaning has been expanded. In particular, most of the useful substances derived from plants have physiological activities such as anti-virus, anti-bacteria, anti-cancer, and anti-oxidation ability, and are attracting attention as ideal resources that can be developed into new drugs. Research on the relationship between the chemical structure and activity of substances is actively being carried out. [0003] However, it is true that the development of physiologically active substances into pharmaceuticals is difficult, and the main reasons for this are as follows. First, the content of physiologically active ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12N5/02
CPCA01H4/005A01H1/02C12N5/04
Inventor 刘永美金昭软陈荣雨李银庆洪宣美
Owner UNHWA CORP
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