13 results about "Cell growth rate" patented technology

A method of facilitating a growth assessment for a cell line includes generating an image of a well that contains a medium that was inoculated with at least one cell of the cell line. The method also includes generating a down-sampled segmentation map comprising pixels that indicate an inferred presence or absence of a cell colony in corresponding portions of the well image. Generating the down-sampled segmentation map includes inputting the well image to a fully convolutional neural network having a plurality of convolutional layers. The method also includes (i) determining, by inputting colony size information (including the down-sampled segmentation map and / or a pixel count derived therefrom) to a cell growth assessment algorithm, a growth classification or score for the cell line, and causing a display of the growth classification or score, and / or (ii) causing a display of the colony size information to facilitate a manual cell growth assessment.

The present technique pertains to a method of preparation of a succinylated collagen-fibrinogen hydrogel and a succinylated collagen-fibrinogen hydrogel prepared thereby. The method of preparation of a succinylated collagen-fibrinogen hydrogel according to the present technique can produce a collagen-based bio-substance in a simple process, wherein collagen can be prevented from being entangled and an improvement can be brought about in hydrophilicity, cell growth rate, cell compatibility, and cell diffusion capability, whereby the collagen-based bio-substance exhibits excellent biocompatibility in vivo.

This invention relates to a laser irradiation device for accelerating cell growth in fermentation broth and its application method in a fermenter. The laser irradiation device includes a laser source module, a light energy transmission system, and an integrated control and synchronization system. The laser source module is located outside the fermenter and generates one or more laser beams with preset parameters. The light energy transmission system guides the laser generated by the laser source module into the fermenter. The integrated control and synchronization system enables intelligent coordinated control of the laser irradiation and fermentation process. Through a rational optical path design and intelligent control system, this device uniformly, safely, and controllably introduces laser energy with specific parameters into a sealed fermenter, thereby effectively regulating cell growth rate and metabolic activity, achieving the goal of shortening the fermentation cycle and increasing biomass or the yield of the target product.

An apparatus for monitoring cell cultures includes a resonant sensor having a cell transduction membrane for contact with cell culture media, a biocompatible sheet overlaid by the cell transduction membrane, a resonant sheet defining a coil pattern, the resonant sheet overlayed by the biocompatible sheet, a plurality of channels formed in the biocompatible sheet, the plurality channels of having a kerf. The kerf for the plurality of channels is selected such that the resonant sensor is configured to capture exponential phase of cell growth including late exponential cell growth and further capture stationary phases of cell growth for the cell cultures.

The invention discloses a polymer gel for organoid culture and a preparation method thereof, and belongs to the technical field of biomedical materials, the gel is formed by cross-linking a phenylboronic acid grafted oxidized sodium alginate solution and a dopamine modified polyvinyl alcohol and cysteine modified gelatin mixed solution. A dynamic boric acid ester bond and enzymolysis network dual-crosslinking system is constructed, the pH-mechanical self-adaptive function is achieved, and the physical limitation of organoid growth is effectively relieved; by utilizing a phenol-sulfydryl synergistic dual anti-oxidation mechanism, active oxygen is removed, and organoid core necrosis is inhibited; a biological enzymolysis mechanism is introduced, so that the problem that gel degradation is mismatched with the cell growth rate is solved. The invention provides an excellent matrix material for standard and large-scale culture of organoids.

An apparatus for monitoring cell cultures includes a resonant sensor having a cell transduction membrane for contact with cell culture media, a biocompatible sheet overlaid by the cell transduction membrane, a resonant sheet defining a coil pattern, the resonant sheet overlayed by the biocompatible sheet, a plurality of channels formed in the biocompatible sheet, the plurality channels of having a kerf. The kerf for the plurality of channels is selected such that the resonant sensor is configured to capture exponential phase of cell growth including late exponential cell growth and further capture stationary phases of cell growth for the cell cultures.

This invention discloses a spatiotemporal tissue dynamics modeling method based on graph neural networks and imbalanced optimal transport. Belonging to the field of bioinformatics, this invention acquires spatial transcriptome data from multiple time points, constructs a low-dimensional latent space for gene expression, and aligns the spatial coordinates. It models the continuous evolution of gene expression states using graph neural differential equations; calculates cell growth rate using imbalanced optimal transport to obtain information on cell number changes; and constructs a spatiotemporal vector field integrating gene expression, cell number, and spatial coordinates to predict tissue state at target time points. This invention achieves collaborative and continuous modeling of cell state, spatial location, and population size during tissue development and regeneration, enabling generative prediction of tissue conformations at future unobserved time points. Furthermore, it is robust to spatial transcriptome data at different resolutions, providing an efficient and reliable computational analysis tool for developmental biology and regenerative medicine research.

The application provides a method for improving the fermentation yield of lysine and pentanediamine, which is a method for dynamically regulating the content of NADH and NADPH in cells, the expression of a gapA gene in a corynebacterium glutamicum is controlled by using a growth-regulated promoter Pald, and the expression of an additionally introduced gapN gene is controlled by using a Psod promoter, so that the expression of gapA is strong in the early growth stage, thereby maintaining the normal NADH concentration in the cells and the cell growth rate, and in the middle and late exponential growth stage and the cell growth stable stage, due to the sharp decrease in the expression strength of Pald and the sharp increase in the expression strength of Psod, gapN is highly expressed, so that the NADPH concentration in the cells is significantly increased, thereby significantly improving the yield and the rate of lysine, and the strategy is also applicable to significantly improving the yield of pentanediamine.

This invention provides a non-human vertebrate exhibiting a higher human liver cell growth rate, a higher human liver cell replacement rate, and higher histological-physiological human reproducibility than existing non-human vertebrates comprising the human liver transplanted therein and a method for producing such non-human vertebrate. Specifically, the method for producing a transgenic non-human vertebrate comprising human liver cells transplanted therein comprises transplanting human liver cells in a non-human vertebrate that has impaired or lowered immune reactions against humans in the presence of human IL-6 in vivo.

The invention discloses an HDAC8 gene knockout MDBK cell line as well as a construction method and application thereof. According to the method, a CRISPR / Cas9 technology is utilized, specific gRNA is designed aiming at a second exon of a cattle HDAC8 gene, CRISPR expression plasmids are constructed, adherent MDBK cells are transfected, and HDAC8 homozygous frame-shift mutant cell lines KOHDAC8-1 (4bp deletion) and KOHDAC8-2 (1bp insertion) are obtained through puromycin screening and monoclonal separation. An identification result shows that HDAC8 knockout obviously promotes replication of the bovine viral diarrhea virus (BVDV), the expression level of NS3 mRNA and protein of the virus is increased, the virus titer is obviously improved, and the cell growth rate is not obviously influenced. The cell line provided by the invention can be used as an efficient proliferation matrix of the BVDV, is used for producing inactivated vaccines for bovine viral diarrhea, and effectively improves the yield and quality of the vaccines.

The invention belongs to the technical field of biology, and discloses construction and application of an artificially synthesized starch recombinant strain. According to the invention, starch synthase is over-expressed in the bacterial cellulose high-yield strain, a competitive pathway of starch synthesis is blocked, and the bacterial cellulose high-yield strain is transformed into an artificial starch synthesis strain. Compared with an artificial starch synthesis path in the prior art, the method has the advantages of low cost, high starch yield, high chassis cell growth rate, sufficient starch synthesis precursor substances and the like.