4 results about "Cell growth rate" patented technology

This invention relates to a laser irradiation device for accelerating cell growth in fermentation broth and its application method in a fermenter. The laser irradiation device includes a laser source module, a light energy transmission system, and an integrated control and synchronization system. The laser source module is located outside the fermenter and generates one or more laser beams with preset parameters. The light energy transmission system guides the laser generated by the laser source module into the fermenter. The integrated control and synchronization system enables intelligent coordinated control of the laser irradiation and fermentation process. Through a rational optical path design and intelligent control system, this device uniformly, safely, and controllably introduces laser energy with specific parameters into a sealed fermenter, thereby effectively regulating cell growth rate and metabolic activity, achieving the goal of shortening the fermentation cycle and increasing biomass or the yield of the target product.

This invention discloses a spatiotemporal tissue dynamics modeling method based on graph neural networks and imbalanced optimal transport. Belonging to the field of bioinformatics, this invention acquires spatial transcriptome data from multiple time points, constructs a low-dimensional latent space for gene expression, and aligns the spatial coordinates. It models the continuous evolution of gene expression states using graph neural differential equations; calculates cell growth rate using imbalanced optimal transport to obtain information on cell number changes; and constructs a spatiotemporal vector field integrating gene expression, cell number, and spatial coordinates to predict tissue state at target time points. This invention achieves collaborative and continuous modeling of cell state, spatial location, and population size during tissue development and regeneration, enabling generative prediction of tissue conformations at future unobserved time points. Furthermore, it is robust to spatial transcriptome data at different resolutions, providing an efficient and reliable computational analysis tool for developmental biology and regenerative medicine research.

The application provides a method for improving the fermentation yield of lysine and pentanediamine, which is a method for dynamically regulating the content of NADH and NADPH in cells, the expression of a gapA gene in a corynebacterium glutamicum is controlled by using a growth-regulated promoter Pald, and the expression of an additionally introduced gapN gene is controlled by using a Psod promoter, so that the expression of gapA is strong in the early growth stage, thereby maintaining the normal NADH concentration in the cells and the cell growth rate, and in the middle and late exponential growth stage and the cell growth stable stage, due to the sharp decrease in the expression strength of Pald and the sharp increase in the expression strength of Psod, gapN is highly expressed, so that the NADPH concentration in the cells is significantly increased, thereby significantly improving the yield and the rate of lysine, and the strategy is also applicable to significantly improving the yield of pentanediamine.

This invention provides a non-human vertebrate exhibiting a higher human liver cell growth rate, a higher human liver cell replacement rate, and higher histological-physiological human reproducibility than existing non-human vertebrates comprising the human liver transplanted therein and a method for producing such non-human vertebrate. Specifically, the method for producing a transgenic non-human vertebrate comprising human liver cells transplanted therein comprises transplanting human liver cells in a non-human vertebrate that has impaired or lowered immune reactions against humans in the presence of human IL-6 in vivo.