24 results about "Cell growth rate" patented technology

An automatic material supplementing method based on a real-time dynamic measurement result, the method comprising: collecting a real-time dynamic measurement result, and calculating a change rate of each component within a unit time, wherein the change rate is a consumption rate or a generation rate; selecting a component having the highest change rate as a marker within a current time period; collecting historical measurement data, and calculating a cell growth rate in a logarithmic growth phase and a target product expression rate in a cultivation and harvesting stage; taking, as a set component concentration value within the current time period of the logarithmic growth phase, a concentration level of the marker when the cell growth rate in the logarithmic growth phase is the highest; taking, as a set component concentration value within the current time period of the cultivation and harvesting stage, a concentration level of the marker when the target product expression rate in the cultivation and harvesting stage is the highest; and calculating an addition dosage of the marker within the current time period on the basis of the change rate of the marker and / or the difference between a real-time measured concentration of the marker within the current time period and the set component concentration value within the current time period, and calculating an addition dosage of a culture medium on the basis of the addition dosage of the marker, so as to obtain a material supplementing strategy.

The invention relates to sgRNAs for demethylation editing of an fgf2 gene of oryzias melastigma, an editing system and application, and belongs to the technical field of gene editing, and the sgRNAs are sgRNA1 + sgRNA2 + sgRNA3 or sgRNA2 + sgRNA3 + sgRNA4 + sgRNA5; the system comprises a Fuw-UbC-dCas9-tet1 plasmid and a pGL3-U6-sgRNAs plasmid, and the sequence of a conserved structural domain of a tet1 gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). Through co-transfection of Mix1 or Mix2, the DNA methylation level of the fgf2 gene promoter can be effectively reduced by about 20.41% at most, the off-target efficiency is extremely low, the cell growth speed is remarkably increased, and stable inheritance is achieved.

A method of facilitating a growth assessment for a cell line includes generating an image of a well that contains a medium that was inoculated with at least one cell of the cell line. The method also includes generating a down-sampled segmentation map comprising pixels that indicate an inferred presence or absence of a cell colony in corresponding portions of the well image. Generating the down-sampled segmentation map includes inputting the well image to a fully convolutional neural network having a plurality of convolutional layers. The method also includes (i) determining, by inputting colony size information (including the down-sampled segmentation map and / or a pixel count derived therefrom) to a cell growth assessment algorithm, a growth classification or score for the cell line, and causing a display of the growth classification or score, and / or (ii) causing a display of the colony size information to facilitate a manual cell growth assessment.

The invention relates to the technical field of bioinformatics, in particular to a cell growth state recognition system, which comprises a growth data time sequence construction module for acquiring original cell population growth data under different drug disturbance conditions, sequentially arranging timestamps and cell observation values in the original cell population growth data, and constructing a cell growth state recognition module; and converting to obtain a cell growth curve calibration result. The method comprises the following steps of: sequencing timestamps and cell observation values of original cell population growth data, establishing a cell growth curve calibration result, further extracting an instantaneous growth rate sequence in a continuous time interval on the basis, positioning the maximum cell growth rate, and capturing a microscopic change trend in a cell dynamic proliferation process; and quantifying a cell saturation density result by using the maximum cell growth rate and the curve end point trend, thereby reflecting the real state of the cell growth in the saturation stable stage.

The present technique pertains to a method of preparation of a succinylated collagen-fibrinogen hydrogel and a succinylated collagen-fibrinogen hydrogel prepared thereby. The method of preparation of a succinylated collagen-fibrinogen hydrogel according to the present technique can produce a collagen-based bio-substance in a simple process, wherein collagen can be prevented from being entangled and an improvement can be brought about in hydrophilicity, cell growth rate, cell compatibility, and cell diffusion capability, whereby the collagen-based bio-substance exhibits excellent biocompatibility in vivo.

This invention relates to a laser irradiation device for accelerating cell growth in fermentation broth and its application method in a fermenter. The laser irradiation device includes a laser source module, a light energy transmission system, and an integrated control and synchronization system. The laser source module is located outside the fermenter and generates one or more laser beams with preset parameters. The light energy transmission system guides the laser generated by the laser source module into the fermenter. The integrated control and synchronization system enables intelligent coordinated control of the laser irradiation and fermentation process. Through a rational optical path design and intelligent control system, this device uniformly, safely, and controllably introduces laser energy with specific parameters into a sealed fermenter, thereby effectively regulating cell growth rate and metabolic activity, achieving the goal of shortening the fermentation cycle and increasing biomass or the yield of the target product.

The invention discloses an SSR4 gene edited CHO (Chinese Hamster Ovary) cell line as well as a preparation method and application thereof. The preservation number of the cell line is CCTCC (China Center For Type Culture Collection) NO: C2025176. An endogenous strong promoter (CMV) and a resistance selection marker are carried by a lentiviral vector, stable high expression (greater than 5 times) of SSR4 in CHO-S cells is realized, the cell growth rate is not influenced, the processing efficiency of glycoprotein in endoplasmic reticulum is further optimized, the proportion of HM glycotypes is remarkably reduced, excessive intervention of a traditional method on end modification of a carbohydrate chain is avoided, and the method has a good application prospect. Finally, accurate control of antibody drug glycosylation modification and stability improvement among batches are achieved, scientific innovation and industrial practicability are achieved, and a technical foundation is laid for high-quality production of next-generation antibody drugs.

An apparatus for monitoring cell cultures includes a resonant sensor having a cell transduction membrane for contact with cell culture media, a biocompatible sheet overlaid by the cell transduction membrane, a resonant sheet defining a coil pattern, the resonant sheet overlayed by the biocompatible sheet, a plurality of channels formed in the biocompatible sheet, the plurality channels of having a kerf. The kerf for the plurality of channels is selected such that the resonant sensor is configured to capture exponential phase of cell growth including late exponential cell growth and further capture stationary phases of cell growth for the cell cultures.

The invention discloses a polymer gel for organoid culture and a preparation method thereof, and belongs to the technical field of biomedical materials, the gel is formed by cross-linking a phenylboronic acid grafted oxidized sodium alginate solution and a dopamine modified polyvinyl alcohol and cysteine modified gelatin mixed solution. A dynamic boric acid ester bond and enzymolysis network dual-crosslinking system is constructed, the pH-mechanical self-adaptive function is achieved, and the physical limitation of organoid growth is effectively relieved; by utilizing a phenol-sulfydryl synergistic dual anti-oxidation mechanism, active oxygen is removed, and organoid core necrosis is inhibited; a biological enzymolysis mechanism is introduced, so that the problem that gel degradation is mismatched with the cell growth rate is solved. The invention provides an excellent matrix material for standard and large-scale culture of organoids.

An apparatus for monitoring cell cultures includes a resonant sensor having a cell transduction membrane for contact with cell culture media, a biocompatible sheet overlaid by the cell transduction membrane, a resonant sheet defining a coil pattern, the resonant sheet overlayed by the biocompatible sheet, a plurality of channels formed in the biocompatible sheet, the plurality channels of having a kerf. The kerf for the plurality of channels is selected such that the resonant sensor is configured to capture exponential phase of cell growth including late exponential cell growth and further capture stationary phases of cell growth for the cell cultures.

This invention discloses a spatiotemporal tissue dynamics modeling method based on graph neural networks and imbalanced optimal transport. Belonging to the field of bioinformatics, this invention acquires spatial transcriptome data from multiple time points, constructs a low-dimensional latent space for gene expression, and aligns the spatial coordinates. It models the continuous evolution of gene expression states using graph neural differential equations; calculates cell growth rate using imbalanced optimal transport to obtain information on cell number changes; and constructs a spatiotemporal vector field integrating gene expression, cell number, and spatial coordinates to predict tissue state at target time points. This invention achieves collaborative and continuous modeling of cell state, spatial location, and population size during tissue development and regeneration, enabling generative prediction of tissue conformations at future unobserved time points. Furthermore, it is robust to spatial transcriptome data at different resolutions, providing an efficient and reliable computational analysis tool for developmental biology and regenerative medicine research.

The invention provides application of myricetin-3-o-galactoside in induction of casein expression in mammary epithelial cells, and belongs to the technical field of cell culture. The invention provides an application of myricetin-3-o-galactoside in at least one of the following aspects: promoting cell growth, inducing casein expression in cells and constructing a casein expression dominant cell model. The myricetin-3-o-galactoside can be used for inducing the expression of casein in cells and promoting the growth of the cells. The embodiment of the invention shows that after myricetin-3-o-galactoside is added into the culture medium, the casein expression quantity in mammary epithelial cells is improved by 5.1 times, and the cell growth rate is also obviously improved. In addition, the method is high in repeatability and stable in induction effect, and a new path is provided for improving the yield and quality of dairy products.

The present invention discloses a dipeptide hydrogel and its application as a cell culture matrix, belonging to the field of materials science. The present invention designs and assembles a dipeptide hydrogel, which is co-assembled by self-assembly of phenylalanine dipeptide modified with 9-fluorenylmethoxycarbonyl protecting group (Fmoc), and then adding tetracarboxyphenyl porphyrin (TCPP) and distearoyl phosphatidylethanolamine (DSPE). When the dipeptide hydrogel prepared by the present invention is used as a cell culture matrix, it can improve the proliferation activity of cultured cells, thereby increasing the cell growth rate and density, and has good biosafety. It provides the possibility for promoting the development of the cell culture field to a higher level.

The application provides a method for improving the fermentation yield of lysine and pentanediamine, which is a method for dynamically regulating the content of NADH and NADPH in cells, the expression of a gapA gene in a corynebacterium glutamicum is controlled by using a growth-regulated promoter Pald, and the expression of an additionally introduced gapN gene is controlled by using a Psod promoter, so that the expression of gapA is strong in the early growth stage, thereby maintaining the normal NADH concentration in the cells and the cell growth rate, and in the middle and late exponential growth stage and the cell growth stable stage, due to the sharp decrease in the expression strength of Pald and the sharp increase in the expression strength of Psod, gapN is highly expressed, so that the NADPH concentration in the cells is significantly increased, thereby significantly improving the yield and the rate of lysine, and the strategy is also applicable to significantly improving the yield of pentanediamine.

The invention discloses a multi-chamber automatic adjustment environment control method and system for cell culture, which are characterized in that each cell culture chamber can be monitored in real time by adjusting the position of a sensor, so that the data accuracy of subsequent model modeling is improved, and the problem that part of regions are difficult to monitor is solved; besides, an optimization algorithm is fused on the basis of model establishment, so that environmental parameters can be automatically adjusted and optimized, and the cell growth rate is increased; the growth environment parameters of each cell culture room are ensured to be in an optimal state, and the stability and repeatability of cell culture are remarkably improved.

This invention provides a non-human vertebrate exhibiting a higher human liver cell growth rate, a higher human liver cell replacement rate, and higher histological-physiological human reproducibility than existing non-human vertebrates comprising the human liver transplanted therein and a method for producing such non-human vertebrate. Specifically, the method for producing a transgenic non-human vertebrate comprising human liver cells transplanted therein comprises transplanting human liver cells in a non-human vertebrate that has impaired or lowered immune reactions against humans in the presence of human IL-6 in vivo.

The invention discloses an HDAC8 gene knockout MDBK cell line as well as a construction method and application thereof. According to the method, a CRISPR / Cas9 technology is utilized, specific gRNA is designed aiming at a second exon of a cattle HDAC8 gene, CRISPR expression plasmids are constructed, adherent MDBK cells are transfected, and HDAC8 homozygous frame-shift mutant cell lines KOHDAC8-1 (4bp deletion) and KOHDAC8-2 (1bp insertion) are obtained through puromycin screening and monoclonal separation. An identification result shows that HDAC8 knockout obviously promotes replication of the bovine viral diarrhea virus (BVDV), the expression level of NS3 mRNA and protein of the virus is increased, the virus titer is obviously improved, and the cell growth rate is not obviously influenced. The cell line provided by the invention can be used as an efficient proliferation matrix of the BVDV, is used for producing inactivated vaccines for bovine viral diarrhea, and effectively improves the yield and quality of the vaccines.

A method of facilitating a growth assessment for a cell line includes generating an image of a well that contains a medium that was inoculated with at least one cell of the cell line. The method also includes generating a down-sampled segmentation map comprising pixels that indicate an inferred presence or absence of a cell colony in corresponding portions of the well image. Generating the down-sampled segmentation map includes inputting the well image to a fully convolutional neural network having a plurality of convolutional layers. The method also includes (i) determining, by inputting colony size information (including the down-sampled segmentation map and / or a pixel count derived therefrom) to a cell growth assessment algorithm, a growth classification or score for the cell line, and causing a display of the growth classification or score, and / or (II) causing a display of the colony size information to facilitate a manual cell growth assessment.

The invention discloses an application of plant-derived miRNA in preparation of antitumor drugs, and belongs to the technical field of biological medicines and functional nucleic acid molecules. The miRNA is mul-miRNA-n16 from mulberry (Morus alba), and the nucleotide sequence of the miRNA is as shown in SEQ ID No: 1; in-vitro experiments prove that overexpression of mul-miRNA-n16 in a liver cancer cell line (such as HepG2) can significantly inhibit cell proliferation and reduce the cell growth rate; meanwhile, the migration and invasion ability of liver cancer cells can be remarkably inhibited, and it is prompted that the polypeptide possibly participates in regulation and control of diffusion and metastasis of tumors through a plurality of signal channels; in a HepG2 tumor-bearing mouse model, local delivery of the mul-miRNA-n16 simulant can also effectively inhibit tumor volume increase, and it is verified that the mul-miRNA-n16 has antitumor activity in vivo; the invention discloses the functional application of mul-miRNA-n16 and the mimic thereof in inhibiting the tumor process for the first time, and provides a new research thought and application basis for developing plant-derived anti-tumor molecules with a novel action mechanism, low toxic and side effects and high targeting property.

The invention provides an automatic material supplementing method based on a real-time dynamic detection result, which comprises the following steps: (1) collecting the real-time dynamic detection result, and calculating the consumption rate and the generation rate of each component in unit time; selecting the component with the fastest consumption rate as a marker of the current time period; (2) collecting historical detection data, and calculating a cell growth rate in a logarithmic phase and a target product expression rate in a culture harvesting stage; taking the concentration level of the marker when the cell growth rate parameter in the logarithmic phase is maximum as a component concentration set value of the current time period of the logarithmic phase; taking the concentration level of the marker when the target product expression rate parameter of the culture harvesting stage is maximum as a component concentration set value of the current time period of the culture harvesting stage; and (3) calculating the additive amount of the marker in the current time period according to the consumption rate and the generation rate of the marker, and calculating the additive amount of the culture medium according to the additive amount of the marker to obtain a feeding strategy.

The invention provides a culture method for producing Eptinezumab in a mammalian cell culture, by comprising culturing mammalian cells, e.g., CHO cells in a bioreactor, e.g., a fed-batch bioreactor, wherein said mammalian cells comprise nucleic acid sequences which provide for the expression of Eptinezumab and wherein culturing is effected at a temperature between 33° C to 38º C and wherein the pH of about 7 (e.g., between 6.85 and 7.05), e.g., wherein the pH is maintained at about 7 for up to 14 days, or at 6.95. The inventive culturing method controls or enhances the cell growth rate, cell viability, viable cell density and / or antibody titer (Eptinezumab) of the mammalian cells.

To provide a cell culture process search method by which optimum culture conditions can be searched, a cell culture process search program, and a cell culture process search device.SOLUTION: The cell culture process search method includes a process condition generation step of generating a plurality of process conditions, a culture result prediction step of acquiring a culture prediction result of cells for each of the generated process conditions, the step including a simulation method including a metabolic flux analysis or a flux balance analysis using a mechanism of cells or a genome-scale metabolic model, and a step of finding an optimal process condition from the culture prediction result, and an optimized process condition acquisition step of selecting an optimal medium composition and culture condition on the basis of a proliferation amount of cells or a bioproduction generation amount of an antibody, and in the optimized process condition acquisition step, the generated process condition and the culture prediction result are input, and an optimal process is calculated using a regression model which is a learned model by machine learning.SELECTED DRAWING: Figure 7

The invention belongs to the technical field of biology, and discloses construction and application of an artificially synthesized starch recombinant strain. According to the invention, starch synthase is over-expressed in the bacterial cellulose high-yield strain, a competitive pathway of starch synthesis is blocked, and the bacterial cellulose high-yield strain is transformed into an artificial starch synthesis strain. Compared with an artificial starch synthesis path in the prior art, the method has the advantages of low cost, high starch yield, high chassis cell growth rate, sufficient starch synthesis precursor substances and the like.

The present invention discloses a method for producing erythritol through continuous fermentation. It relates to the field of fermentation engineering technology. The method comprises placing a seed liquid in a culture medium and establishing a continuous fermentation mechanism with CO2 feedback control. The present invention produces erythritol by continuous fermentation, and in the late fermentation stage, feeds are added and discharged simultaneously to maintain a constant glucose and volume of the fermentation liquid. In the continuous fermentation stage, the feed is discharged at the same rate as the feed. On the one hand, by releasing part of the bacterial cells, the growth rate of the bacterial cells is reduced, the consumption of the substrate glucose by bacterial growth is reduced, and the conversion rate of erythritol is improved. On the other hand, the bacterial cells can be reused multiple times, shortening the fermentation cycle of new tanks. In addition, combined with tail gas analysis, the yeast growth status is timely judged. Based on the tail carbon value as the control parameter, the respiratory status of the bacterial cells during the fermentation process is analyzed. By adjusting the feed flow acceleration rate, the bacterial cell growth rate and respiratory frequency are effectively controlled, thereby promoting an increase in the conversion rate of glucose to erythritol.